KEGG ENZYME: 2.1.1.46

ENZYME: 2.1.1.46

Entry

EC 2.1.1.46                 Enzyme

Name

isoflavone 4'-O-methyltransferase;
4'-hydroxyisoflavone methyltransferase;
isoflavone methyltransferase;
isoflavone O-methyltransferase

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase

Reaction(IUBMB)

S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]

Reaction(KEGG)

R07331 > R02931 R06564

Substrate

S-adenosyl-L-methionine [CPD:C00019];
4'-hydroxyisoflavone [CPD:C22375]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
4'-methoxyisoflavone [CPD:C03074]

Comment

Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.

History

EC 2.1.1.46 created 1976, modified 2011

Pathway

ec00943

Isoflavonoid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K13259

2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase / isoflavone 4'-O-methyltransferase

Genes

GMX:	100807589(IOMT1) 106795493(IOMT2)

GSJ:	114374101 114374126

PVU:	PHAVU_003G051600g

VRA:

106780380

VAR:

108341659

VUN:

114175643

VUM:	124837707 124845747

CCAJ:

109816528

APRC:

113858334

MTR:

25493072

TPRA:

123881701 123913950 123913951

CAM:	101494507 101494823 101495151

PSAT:

127108027

LJA:	130711100 130711801

ADU:

107480758

AIP:

107631191

AHF:	112733553 112791519

LANG:

109349376

» show all

Reference

1 [PMID:4452353]

Authors

Wengenmayer H, Ebel J, Grisebach H.

Title

Purification and properties of a S-adenosylmethionine: isoflavone 4'-O-methyltransferase from cell suspension cultures of Cicer arietinum L.

Journal

Eur J Biochem 50:135-43 (1974)
DOI:10.1111/j.1432-1033.1974.tb03881.x

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.46

IUBMB Enzyme Nomenclature:

2.1.1.46

ExPASy - ENZYME nomenclature database:

2.1.1.46

BRENDA, the Enzyme Database:

2.1.1.46

CAS:	55071-80-2

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