Entry
Name
tRNA 5-(aminomethyl)-2-thiouridylate-methyltransferase;
transfer ribonucleate 5-methylaminomethyl-2-thiouridylate 5-methyltransferase;
tRNA 5-methylaminomethyl-2-thiouridylate 5'-methyltransferase;
S-adenosyl-L-methionine:tRNA (5-methylaminomethyl-2-thio-uridylate)-methyltransferase;
tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:tRNA 5-(aminomethyl)-2-thiouridylate N-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + tRNA containing 5-(aminomethyl)-2-thiouridine = S-adenosyl-L-homocysteine + tRNA containing 5-[(methylamino)methyl]-2-thiouridylate [RN:
R00601 ]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:
C00019 ];
tRNA containing 5-(aminomethyl)-2-thiouridine [CPD:
C11478 ]
Product
S-adenosyl-L-homocysteine [CPD:
C00021 ];
tRNA containing 5-[(methylamino)methyl]-2-thiouridylate [CPD:
C04728 ]
Comment
This enzyme specifically adds the terminal methyl group of 5-[(methylamino)methyl]-2-thiouridylate.
History
EC 2.1.1.61 created 1982, modified 2012, modified 2021
Orthology
K15461 tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein
Genes
ECOO : ECRM13514_3083(mnmC)
ECOH : ECRM13516_3026(mnmC)
ELR : ECO55CA74_14180(mnmC)
ECW : EcE24377A_2619(mnmC)
ECOA : APECO78_15440(mnmC)
EDJ : ECDH1ME8569_2262(mnmC)
ECOI : ECOPMV1_02483(mnmC)
SEM : STMDT12_C24010(mnmC)
SETC : CFSAN001921_04890(mnmC)
SEEN : SE451236_18100(mnmC)
SENH : CFSAN002069_19950(mnmC)
SEEH : SEEH1578_21170(mnmC)
SENJ : CFSAN001992_21695(mnmC)
SEEC : CFSAN002050_18825(mnmC)
SEEB : SEEB0189_007675(mnmC)
SENC : SEET0819_18995(mnmC)
REE : electrica_01426(mnmC)
CLAP : NCTC11466_01261(mnmC)
KIE : NCTC12125_00346(mnmC_1)
AHN : NCTC12129_01482(mnmC_1)
METY : MRY16398_15750(mnmC)
PSHI : SAMEA2665130_0918(mnmC)
SMW : SMWW4_v1c34760(mnmC)
SERS : SERRSCBI_16875(mnmC)
SFJ : SAMEA4384070_3486(mnmC)
SOF : NCTC11214_03310(mnmC_2)
SGRI : SGBXF1_03432(mnmC_2)
SSAR : SSARUM_003343(mnmC)
PAQU : DMB82_0007270(mnmC)
PARL : PEC302110_26470(mnmC)
DIC : Dpoa569_001109(mnmC)
TPTY : NCTC11468_02539(mnmC)
PHEI : NCTC12003_01520(mnmC)
PRJ : NCTC6933_02382(mnmC)
LRI : NCTC12151_01160(mnmC)
HIC : NTHIC486_01171(mnmC)
HPIT : NCTC13334_01882(mnmC)
HHZ : NCTC10839_01325(mnmC)
HAEG : NCTC8502_01544(mnmC)
PDAG : 4362423_00206(mnmC)
ALIG : NCTC10568_00438(mnmC)
ASEG : NCTC10977_01029(mnmC)
AVT : NCTC3438_01056(mnmC)
PAET : NCTC13378_00439(mnmC)
VAU : VANGNB10_cI1918(mnmC)
VPL : SA104470976_00706(mnmC)
VIB : VspSTUT11_07670(mnmC)
VIS : VspSTUT16_08670(mnmC)
PAEB : NCGM1900_2813(mnmC)
PPB : PPUBIRD1_3862(mnmC_2)
PVD : CFBP1590__3846(mnmC)
PPRC : PFLCHA0_c44040(mnmC)
PMUD : NCTC8068_01742(mnmC)
PFW : PF1751_v1c38170(mnmC)
PPUU : PputUW4_01339(mnmC)
PTRT : HU722_0020135(mnmC)
PTW : TUM18999_42950(mnmC)
PTAE : NCTC10697_01545(mnmC)
MSHE : MAALD49_21670(mnmC)
PSYC : DABAL43B_2271(mnmC)
MCAT : MC25239_00878(mnmC)
MCUN : NCTC10297_01429(mnmC)
SCAA : TUM17387_16810(mnmC)
SALH : HMF8227_01958(mnmC)
LLG : 44548918_01504(mnmC)
LJR : NCTC11533_01465(mnmC)
LWA : SAMEA4504053_1764(mnmC)
LSS : NCTC12082_00609(mnmC)
LADL : NCTC12735_00659(mnmC)
LANT : TUM19329_19000(mnmC)
TSE : THMIRHAS_01360(mnmC)
TZO : THMIRHAT_20960(mnmC)
TNI : TVNIR_1531(mnmC_[H])
GAI : IMCC3135_25155(mnmC_3)
HAAH : HALA3H3_180022(mnmC)
ALCA : ASALC70_04015(mnmC)
AEL : NCTC12917_01816(mnmC)
CHJ : NCTC10426_02320(mnmC)
CDIZ : CEDIAZO_02084(mnmC_2)
REH : H16_A1481(h16_A1481)
BUE : BRPE67_ACDS27350(mnmC)
PLG : NCTC10937_01662(mnmC)
LMIR : NCTC12852_01904(mnmC_1) NCTC12852_01930(mnmC_2)
BTRM : SAMEA390648700524(mnmC_1)
AXX : ERS451415_00831(mnmC)
LIM : L103DPR2_01892(mnmC_1)
LIH : L63ED372_01714(mnmC_2)
SMIO : CATMQ487_25220(mnmC)
URU : DSM104443_00463(mnmC)
UPL : DSM104440_00531(mnmC)
THAG : CKCBHOJB_01616(mnmC)
FPHO : SHINM1_005120(mnmC)
RBS : RHODOSMS8_00341(mnmC)
BVY : NCTC9239_02746(mnmC)
TSV : DSM104635_00968(mnmC)
SULG : FJR48_05355 FJR48_11280
CJV : MTVDSCj20_1273(mnmC)
CFV : CFVI03293_1205(mnmC)
CAMP : CFT03427_1179(mnmC)
CCOC : CCON33237_1383(mnmC)
CLR : UPTC16701_0621(mnmC)
CLM : UPTC16712_0629(mnmC)
CAMR : CAQ16704_0657(mnmC)
CHV : CHELV3228_0504(mnmC)
CPIN : CPIN18020_1408(mnmC)
CAMZ : CVIC12175_0582(mnmC)
» show all
Taxonomy
Reference
Authors
Taya Y, Nishimura S.
Title
Biosynthesis of 5-methylaminomethyl-2-thiouridylate. I. Isolation of a new tRNA-methylase specific for 5-methylaminomethyl-2-thiouridylate.
Journal
Reference
2
Authors
Taya Y, Nishimura S.
Title
In: Salvatore F, Borek E, Zappia V, Williams-Ashman HG, Schlenk F (eds). The Biochemistry of Adenosylmethionine. New York: Columbia University Press; 1977. p. 251.
Reference
Authors
Bujnicki JM, Oudjama Y, Roovers M, Owczarek S, Caillet J, Droogmans L
Title
Identification of a bifunctional enzyme MnmC involved in the biosynthesis of a hypermodified uridine in the wobble position of tRNA.
Journal
Reference
Authors
Kim J, Almo SC.
Title
Structural basis for hypermodification of the wobble uridine in tRNA by bifunctional enzyme MnmC.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.61
ExPASy - ENZYME nomenclature database: 2.1.1.61