Entry
Name
methylenetetrahydrofolate---tRNA-(uracil54-C5)-methyltransferase [NAD(P)H-oxidizing];
folate-dependent ribothymidyl synthase;
methylenetetrahydrofolate-transfer ribonucleate uracil 5-methyltransferase;
5,10-methylenetetrahydrofolate:tRNA-UPsiC (uracil-5-)-methyl-transferase;
5,10-methylenetetrahydrofolate:tRNA (uracil-5-)-methyl-transferase;
TrmFO;
folate/FAD-dependent tRNA T54 methyltransferase;
methylenetetrahydrofolate---tRNA-(uracil54-C5)-methyltransferase (FADH2-oxidizing)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
5,10-methylenetetrahydrofolate:tRNA (uracil54-C5)-methyltransferase
Reaction(IUBMB)
5,10-methylenetetrahydrofolate + uracil54 in tRNA + NAD(P)H + H+ = tetrahydrofolate + 5-methyluracil54 in tRNA + NAD(P)+ [RN:
R12548 ]
Reaction(KEGG)
Substrate
Product
tetrahydrofolate [CPD:
C00101 ];
5-methyluracil54 in tRNA;
NAD+ [CPD:
C00003 ];
NADP+ [CPD:
C00006 ]
Comment
A flavoprotein (FAD). Up to 25% of the bases in mature tRNA are post-translationally modified or hypermodified. One almost universal post-translational modification is the conversion of U54 into ribothymidine in the TPsiC loop, and this modification is found in most species studied to date [2]. Unlike this enzyme, which uses 5,10-methylenetetrahydrofolate and NAD(P)H to supply the atoms for methylation of U54, EC
2.1.1.35 , tRNA (uracil54-C5)-methyltransferase, uses S-adenosyl-L-methionine.
History
EC 2.1.1.74 created 1983 as EC 2.1.2.12, transferred 1984 to EC 2.1.1.74, modified 2011, modified 2019
Orthology
K04094 methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase
Genes
RBS : RHODOSMS8_01077(trmFO)
SMEL : SM2011_c02053(trmFO)
SFD : USDA257_c35830(trmFO)
SAME : SAMCFNEI73_Ch1677(trmF)
AGR : AGROH133_06540(gidA)
AVV : RvVAT039_01310(trmFO)
AVF : RvVAR031_22460(trmFO)
REC : RHECIAT_CH0001922(gidA1)
REL : REMIM1_CH01885(trmFO)
REP : IE4803_CH01868(trmFO)
REI : IE4771_CH01920(trmFO)
RTR : RTCIAT899_CH07985(trmFO)
RGA : RGR602_CH01708(trmFO)
RHN : AMJ98_CH01884(trmFO)
RPHA : AMC79_CH01963(trmFO)
RHX : AMK02_CH01889(trmFO)
REZ : AMJ99_CH01962(trmFO)
RHID : FFM81_011865(trmFO)
RLW : RlegWSM1455_08450(trmFO)
NEN : NCHU2750_21320(trmFO)
PDES : FE840_012575(trmFO)
BSW : IY71_04460 IY71_12610
BSG : IY72_04190 IY72_12150
BVZ : BRAD3257_4322(trmFO)
BSEP : HAP48_0039040(trmFO)
BQB : J4P68_0038755(trmFO)
BBAN : J4G43_028540(trmFO)
BCAN : BcanWSM471_21155(trmFO)
VGO : GJW-30_1_02900(trmFO)
BHS : BM1374165_00579(gidA1)
BBK : BARBAKC583_0551(gid)
BTX : BM1374166_00819(gidA1)
BEZ : NCTC12898_00528(trmFO)
MTUN : MTUNDRAET4_2471(trmFO)
FIL : BN1229_v1_3297(trmFO)
FIY : BN1229_v1_2624(trmFO)
BVY : NCTC9239_01422(trmFO)
TSV : DSM104635_01690(trmFO)
RLI : RLO149_c019490(trmFO)
PHP : PhaeoP97_01441(trmFO)
PPIC : PhaeoP14_01347(trmFO)
SINL : DSM14862_01358(trmFO)
SPSE : SULPSESMR1_01666(trmFO)
RMM : ROSMUCSMR3_03976(trmFO)
AHT : ANTHELSMS3_03306(trmFO)
LVS : LOKVESSMR4R_01470(trmFO)
AALK : LGT41_0012130(trmFO)
SBIN : SBA_ch1_30080(trmFO)
SFLA : SPHFLASMR4Y_01016(trmFO)
ABS : AZOBR_p120086(trmFO)
SMUC : JL100_016790(trmFO)
SROE : JL101_015645(trmFO)
MGY : MGMSRv2__1351(trmFO)
GBN : GEOBRER4_15200(trmFO)
PSEF : PSDVSF_02740(trmFO)
PPOR : JCM14722_00890(trmFO)
MFB : MFUL124B02_18760(gid)
PAUU : E8A73_025675(trmFO)
BSH : BSU6051_16130(trmFO)
BAML : BAM5036_1533(trmFO)
BAZ : BAMTA208_09085(trmFO)
BMOB : MLA2C4_20030(trmFO)
BMET : BMMGA3_06075(trmFO)
BKW : BkAM31D_14815(trmFO)
PARG : PspKH34_26220(trmFO)
LYC : FH508_0005425(trmFO)
NTM : BTDUT50_07305(trmFO)
MLIT : KDJ21_021260(trmFO)
BLEN : NCTC4824_02678(trmFO)
SUJ : SAA6159_01106(trmFO)
SUQ : HMPREF0772_11979(trmFO)
SUF : SARLGA251_11620(trmFO)
SAUT : SAI1T1_2008940(gid)
SAUJ : SAI2T2_1008960(gid)
SAUK : SAI3T3_1008950(gid)
SAUQ : SAI4T8_1008940(gid)
SAUV : SAI7S6_1008950(trmFO)
SAUW : SAI5S5_1008910(trmFO)
SAUX : SAI6T6_1008920(trmFO)
SAUY : SAI8T7_1008950(trmFO)
SDP : NCTC12225_01765(trmFO)
SCAP : AYP1020_0560(trmFO)
SPIC : SAMEA4384060_1685(gid)
SSH : NCTC13712_01196(trmFO)
SSIM : SAMEA4384339_1135(gid)
SSTE : SAMEA4384403_1560(gid)
LMOC : LMOSLCC5850_1334(gid)
LMOZ : LM1816_15037(trmFO)
LMW : LMOSLCC2755_1279(gid)
LMX : LMOSLCC2372_1337(gid)
LMZ : LMOSLCC2482_1325(gid)
LMON : LMOSLCC2376_1227(gid)
LMOS : LMOSLCC7179_1246(gid)
LMOO : LMOSLCC2378_1291(gid)
LMOY : LMOSLCC2479_1336(gid)
LMOT : LMOSLCC2540_1327(gid)
LMOA : LMOATCC19117_1285(gid)
LGZ : NCTC10812_01686(trmFO)
LMAR : LAX80_006030(trmFO)
GMO : NCTC11323_00831(trmFO)
GHA : NCTC10459_00820(trmFO)
BAGR : BA6348_13170(trmFO)
PCHI : PC41400_21485(trmFO)
COHN : KCTCHS21_38480(gidA)
KZO : NCTC404_00068(trmFO)
SPOR : SporoP33_14215(gid)
SURE : SporoP32a_14835(gid)
PLAY : DNR44_010510(trmFO)
PABS : JIR001_18650(trmFO)
LLX : NCDO2118_1242(trmFO)
SPH : MGAS10270_Spy1007(gid)
SPI : MGAS10750_Spy1042(gid)
SPJ : MGAS2096_Spy0966(gid)
SPK : MGAS9429_Spy1010(gid)
STG : MGAS15252_0891(gidA.1)
STX : MGAS1882_0886(gidA.1)
STZ : SPYALAB49_000884(trmFO)
SNC : HMPREF0837_11547(trmFO)
SGG : SGGBAA2069_c10780(gid)
SCP : HMPREF0833_10518(trmFO)
SVB : NCTC12167_00899(gid)
SMEN : SAMEA4412692_0646(trmFO)
SFER : NCTC12278_00934(trmFO)
SCAI : NCTC12191_01604(gid)
SPSU : NCTC13786_00225(gid)
SPOC : NCTC10925_00943(gid)
SAUP : NCTC3168_01722(gidA_2)
SURN : NCTC13766_01113(trmFO)
SVF : NCTC3166_01025(gidA_1)
LPW : LpgJCM5343_10580(gid)
EFU : HMPREF0351_11138(gidA)
ECEC : NCTC12421_01370(trmFO)
CLO : HMPREF0868_0861(trmFO)
RHER : EHE19_008250(trmFO)
SMAN : C12CBH8_08820(trmFO)
PFAA : MM59RIKEN_23700(gidA)
VFA : MM35RIKEN_21720(trmFO)
THEP : DYI95_004825(trmFO)
MTHO : MOTHE_c09860(trmFO2)
MTHZ : MOTHA_c10750(trmFO2)
CDIA : CaldiYA01_04120(trmFO)
CNAG : OTJ99_000597(trmFO)
CMOR : OTK00_001961(trmFO)
KME : H0A61_02252(trmFO_1)
VRM : 44547418_00986(trmFO)
VDN : NCTC11831_00978(trmFO)
DHO : Dia5BBH33_07200(trmFO)
MANA : MAMMFC1_03906(trmFO)
STED : SPTER_18070(trmFO_2)
PFAC : PFJ30894_01627(trmFO)
AARG : Aargi30884_06960(trmFO)
ABSI : A9CBEGH2_08800(trmFO)
FIT : Fi14EGH31_15600(trmFO)
LCG : L3BBH23_13430(trmFO)
MMY : MSC_0375(gidA) MSC_0494(gid)
MMYM : MMS_A0413(gidA_1) MMS_A0543(trmFO)
MMYI : mycmycITA_00404(gid_1) mycmycITA_00532(gid_2)
MML : MLC_3470(trmFO) MLC_4740(trmFO)
MCP : MCAP_0476(gid) MCAP_0613
MCAC : MCCP01_0560(trmFO) MCCP01_0687(trmFO)
MCAP : MCCPF38_00568(trmFO_1) MCCPF38_00699(trmFO_2)
MCAR : MCCPILRI181_00566(trmFO_1) MCCPILRI181_00701(trmFO_4)
MCAI : MCCG_0559 MCCG_0691
MLC : MSB_A0489(trmFO) MSB_A0618(gidA_1)
MLH : MLEA_002900(trmFO1) MLEA_005830(trmFO2)
MFER : D500_00474(rlmFO) D500_00594(trmFO2)
MPF : MPUT_0257 MPUT_0352(trmFO) MPUT_0540(trmFO)
MPUT : MPUT9231_1810(trmFO) MPUT9231_3940(trmFO2) MPUT9231_4900(trmFO1)
MYT : MYE_01660(trmFO) MYE_02150(trmFO-2) MYE_02640(rlmFO)
MCOT : NX779_01880(trmFO) NX779_02530(trmFO) NX779_03200(trmFO)
MBV : MBOVPG45_0161(gidA-1)
AHK : NCTC10172_01268(gid)
AAXA : NCTC10138_00610(gid)
MFW : mflW37_2500 mflW37_5610
MCHC : CK556_01350 CK556_03025
MENT : CS528_01300 CS528_03045
MSYR : CXP39_01195 CXP39_02435
MTAB : MTABA_v1c02570(gid) MTABA_v1c05820(gid)
MCOL : MCOLE_v1c02630(gid) MCOLE_v1c05550(gid)
ELJ : ELUMI_v1c03790(gid) ELUMI_v1c06950(gid)
ESX : ESOMN_v1c02380(gid) ESOMN_v1c04360(gid)
SCR : SCHRY_v1c01480(gid1) SCHRY_v1c03570(gid2)
SSYR : SSYRP_v1c01680(gid1) SSYRP_v1c03650(gid2)
SDI : SDIMI_v3c02760(gid) SDIMI_v3c06630(gid)
STAI : STAIW_v1c03180(gid) STAIW_v1c09150(gid)
SAPI : SAPIS_v1c01550(gid1) SAPIS_v1c03290(gid2)
SCQ : SCULI_v1c03510(gid1) SCULI_v1c08170(gid2)
SSAB : SSABA_v1c02780(gid1) SSABA_v1c06390(gid2)
SERI : SERIO_v1c02160(gid) SERIO_v1c04500(gid)
SCJ : SCANT_v1c02850(gid) SCANT_v1c08140(gid)
SHJ : SHELI_v1c02870(gid) SHELI_v1c07510(trmFO)
SFZ : SFLOR_v1c03450(gid) SFLOR_v1c08710(gid)
SCOU : SCORR_v1c07550(gid)
SPRN : S100390_v1c01530(trmFO) S100390_v1c03690(gid)
SPIT : STU14_v1c01530(trmFO) STU14_v1c03690(gid)
STAB : STABA_v1c03910(gid) STABA_v1c08490(gid)
SMOO : SMONO_v1c02670(gid) SMONO_v1c06330(gid)
SALX : SALLE_v1c03910(gid) SALLE_v1c06580(gid)
SGQ : SGLAD_v1c03340(gid) SGLAD_v1c07930(trmFO)
SCHI : SCHIN_v1c04590(trmFO) SCHIN_v1c09550(trmFO)
SMEI : SRED_001915 SRED_002124
EYY : EGYY_16810(Gid_(BS))
RTS : CE91St31_16070(trmFO)
SYP : SYNPCC7002_A2394(gidA)
SYNW : SynWH8103_01030(trmFO)
SYNC : CB0101_12330(trmFO)
NSPH : BDGGKGIB_00285(trmFO)
ABAT : CFX1CAM_1251(trmFO)
PBF : CFX0092_A3645(trmFO)
PPIO : CE91St28_16730(trmFO)
» show all
Taxonomy
Reference
Authors
Delk AS, Nagle DP Jr, Rabinowitz JC.
Title
Methylenetetrahydrofolate-dependent biosynthesis of ribothymidine in transfer RNA of Streptococcus faecalis. Evidence for reduction of the 1-carbon unit by FADH2.
Journal
J Biol Chem 255:4387-90 (1980)
Reference
Authors
Becker HF, Motorin Y, Sissler M, Florentz C, Grosjean H.
Title
Major identity determinants for enzymatic formation of ribothymidine and pseudouridine in the T psi-loop of yeast tRNAs.
Journal
Reference
Authors
Nishimasu H, Ishitani R, Yamashita K, Iwashita C, Hirata A, Hori H, Nureki O
Title
Atomic structure of a folate/FAD-dependent tRNA T54 methyltransferase.
Journal
Reference
Authors
Yamagami R, Yamashita K, Nishimasu H, Tomikawa C, Ochi A, Iwashita C, Hirata A, Ishitani R, Nureki O, Hori H
Title
The tRNA recognition mechanism of folate/FAD-dependent tRNA methyltransferase (TrmFO).
Journal
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.74
ExPASy - ENZYME nomenclature database: 2.1.1.74