KEGG ENZYME: 2.1.1.95

ENZYME: 2.1.1.95

Entry

EC 2.1.1.95                 Enzyme

Name

tocopherol C-methyltransferase;
gamma-tocopherol methyltransferase;
VTE4 (gene name);
S-adenosyl-L-methionine:gamma-tocopherol 5-O-methyltransferase (incorrect);
tocopherol O-methyltransferase (incorrect)

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:gamma-tocopherol 5-C-methyltransferase

Reaction(IUBMB)

(1) S-adenosyl-L-methionine + gamma-tocopherol = S-adenosyl-L-homocysteine + alpha-tocopherol  [RN:R07236];
(2) S-adenosyl-L-methionine + delta-tocopherol = S-adenosyl-L-homocysteine + beta-tocopherol  [RN:R07504];
(3) S-adenosyl-L-methionine + gamma-tocotrienol = S-adenosyl-L-homocysteine + alpha-tocotrienol  [RN:R10491];
(4) S-adenosyl-L-methionine + delta-tocotrienol = S-adenosyl-L-homocysteine + beta-tocotrienol [RN:R10492]

Reaction(KEGG)

R07236 R07504 R10491 R10492

Substrate

S-adenosyl-L-methionine [CPD:C00019];
gamma-tocopherol [CPD:C02483];
delta-tocopherol [CPD:C14151];
gamma-tocotrienol [CPD:C14155];
delta-tocotrienol [CPD:C14156]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
alpha-tocopherol [CPD:C02477];
beta-tocopherol [CPD:C14152];
alpha-tocotrienol [CPD:C14153];
beta-tocotrienol [CPD:C14154]

Comment

The enzymes from plants and photosynthetic bacteria have similar efficiency with the gamma and delta isomers of tocopherols and tocotrienols.

History

EC 2.1.1.95 created 1989, modified 2013, modified 2019

Pathway

ec00130

Ubiquinone and other terpenoid-quinone biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K05928

tocopherol O-methyltransferase

Genes

ATH:	AT1G64970(G-TMT)

ALY:

9322850

CRB:

17896175

CSAT:

104722834 104725296 104751124 104760795

EUS:	EUTSA_v10018768mg

BRP:	103852208 103853294

BNA:	106388189 106390439 106406285 125574762 125604823

BOE:	106318945 106322543

RSZ:

108816273

THJ:	104805718 104821707

CPAP:

110815941

CIT:

102628754

CIC:	CICLE_v10005360mg CICLE_v10032026mg

PVY:	116113145 116128697

MINC:

123220265

TCC:

18599602

GRA:	105783674 105787986

GHI:	107920515 107951798 121215348

GAB:	108461158 108463606

HSYR:

120133705 120161646 120208181

DZI:	111276925 111277748 111296546

EGR:

104424262

RARG:

115728394

GMX:	100778509 100792443(GAMMA-TMT) 732543

GSJ:	114378746 114378747 114425831

PVU:	137817856 137818558

VRA:	106779509 106779693

VAR:	108339045 108339528

VUN:	114168461 114168600

VUM:

124827030

CCAJ:

109798298 109798841

APRC:

113864367 113864369

MTR:

25481971

TPRA:

123916282

CAM:

101513488

PSAT:

127091611

VVO:	131596358 131630921

LJA:

130747841

ADU:	107488489 107488490

AIP:	107643212 107643213

AHF:	112749831 112749832 112801308

LANG:

109348558

PCIN:

129312705

QSA:	O6P43_002336 O6P43_002337

FVE:

101307130

AANS:

126797753

RCN:

112192792

PPER:

18769165

PMUM:

103339737

PAVI:

110753538

PDUL:

117633880

MDM:	103414721 103449390

MSYL:

126600160

PXB:	103942764 103944307 103955170

PCOX:

137713323 137744597

ZJU:

107414726

MNT:

21402584

CSAV:

115716613

CSV:

101205148

CMO:

103501078

BHJ:

120087680

MCHA:

111017272

CMAX:

111484262

CMOS:

111449074

CPEP:

111777902

RCU:

8284310

JCU:

105632484

HBR:

110659616

MESC:

110619928

POP:

7465624

PEU:

105139626

PALZ:

118053559

PNZ:

133669837

JRE:

109003796

CILL:

122311772

CAVE:

132167650 132182424

AGLU:

133855630 133855646

QSU:	111988756 112012460

QLO:	115960404 115960524

TWL:

120016222

VVI:

100242641

VRI:	117931601 117932870

SLY:	778248(TMT)

SPEN:

107028652

SOT:	102577815(GAMMA-TMT)

SSTN:

125842189

SDUL:

129889656

CANN:

107870898

LBB:

132635354

NTA:	107793279 107804296 107809106

NSY:	104241965 104247874

NTO:

104111647

NAU:

109211043

INI:

109179117

ITR:	116012093 116013898

SIND:

105174312

OEU:

111381589

EGT:

105961226

SSPL:

121792242 121799119

SMIL:

130997728

SHIS:

125217603

APAN:

127250980

HAN:	110872346 110872347 110890669

ECAD:

122603529

LSV:	111900764 111916272

CCAV:

112512163 112524460 112528192

DCR:

108210384

CSIN:

114270934 114270949 114316857

RVL:	131300026 131301495

AEW:	130767209 130789869

DLT:	127804134 127804138

BVG:

104888087

SOE:

110793113

CQI:

110693052

ATRI:

130808217 130823885 130825775

MOF:

131159274

NNU:	104590834 104590835 104590913 104611563

MING:

122081156

TSS:

122646314

PSOM:

113347964 113349396

OSA:	4330437(G-TMT)

DOSA:

Os02g0701600

OBR:

102706446

OGL:

127764584

BDI:

100836877

ATS:

109744847

TDC:	119317549 119323408

TAES:

123128104 123137891 780621

TUA:

125514295

LPER:

127306535

LRD:

124692933

SBI:	8067143 8073167 8079111

ZMA:

732837

MFLO:

136464230 136474631

SITA:

101778567

SVS:

117834521

PVIR:

120656021

PHAI:

112881382

PAUA:

133919600 133921443

PDA:

103716652

EGU:

105033221

MUS:

104000155

ZOF:	121975977 122011815 122017426 122034823

DCT:

110115783

PEQ:

110031053

AOF:	109837030 109838387

MSIN:

131247894

ACAK:

137933949

NCOL:

116253313 116265400 116266046

ATR:	110006333 18443978

CJF:

131055531

SMO:	SELMODRAFT_145351 SELMODRAFT_167817 SELMODRAFT_173430 SELMODRAFT_175008 SELMODRAFT_235738 SELMODRAFT_451558 SELMODRAFT_89835 SELMODRAFT_93926

PPP:

112288826

CRE:	CHLRE_09g393400v5

VCN:	VOLCADRAFT_105162

MNG:

MNEG_4528

CSL:	COCSUDRAFT_33371

CVR:	CHLNCDRAFT_26131

APRO:

F751_6137

OLU:	OSTLU_40586

OTA:	OT_ostta16g00380

BPG:	Bathy07g04690

MIS:	MICPUN_59038

MPP:	MICPUCDRAFT_34805

CME:	CYME_CMT560C

GSL:	Gasu_24450

CCP:	CHC_T00000837001

PTI:	PHATRDRAFT_20470(TMT)

FCY:	FRACYDRAFT_238951(VTE4)

TPS:	THAPSDRAFT_23931

AAF:	AURANDRAFT_3921

EHX:	EMIHUDRAFT_60986

GTT:	GUITHDRAFT_71036

ASIP:

AQUSIP_02080

TMC:

LMI_3088

CCOS:

Pan44_29410(rebM_2)

PVAR:

SH412_005372

ALAC:

JYE50_01160

SABD:

N8I86_30045

KIS:	HUT16_31035

ABRY:

NYE86_02835

PQA:	WHH00_09785

ALO:

CRK55118

MSAG:

GCM10017556_09930

MCAY:

PVK37_30210

CATI:

CS0771_28010

DVC:	Dvina_13845

DFU:	Dfulv_12490

DAUR:

Daura_12980

DMAT:

Dmats_13380

DACT:

KZZ52_12715

CAI:

Caci_3249

SYN:	slr0089(erg6)

SYZ:	MYO_126250(erg6)

SYY:	SYNGTS_2600(slr0089)

SYT:	SYNGTI_2599(slr0089)

SYS:	SYNPCCN_2598(slr0089)

SYQ:	SYNPCCP_2598(slr0089)

SYJ:	D082_21800(erg6)

SYO:	C7I86_13605

SYNH:

HTZ78_03150

CYA:

CYA_2254

CYB:

CYB_2403

SYNE:

Syn6312_1639

SYNP:

Syn7502_02171

WNA:	KA717_38800

CMP:	Cha6605_5217

CYN:	Cyan7425_3207

CEP:	Cri9333_1617

AMR:

AM1_4540

ACAM:

HRE53_20135

LEN:	LEP3755_11130

LET:	O77CONTIG1_00395(rebM_1)

LBO:	LBWT_42280

LEPT:

JVX88_14965

LEPB:

GFS31_05820

LSC:	KIK02_03895

LSK:	J5X98_20985

SFRI:

RBJ76_22925

HHG:	XM38_016580

DSL:	Dacsa_1481

PSEU:

Pse7367_2506

THEU:

HPC62_08070

TOG:	HNI00_19845

TSIN:

OXH18_01975

GLP:	Glo7428_4760

CHON:

NIES4102_02280

GDU:	P0S91_08870

SYP:	SYNPCC7002_A1791

SYV:	AWQ23_09130

SYL:	AWQ21_09475

LEP:	Lepto7376_4312

SYNN:

NIES970_17770

PIP:	FEK30_13405

MAR:

MAE_24880

MPK:

VL20_4041

MIQ:	B5D77_14925

MVZ:	myaer102_01730

HAO:	PCC7418_1397

ENN:	FRE64_10070

CYT:

cce_3547

CWA:	CwatDRAFT_0798

CYP:	PCC8801_0328

CYH:	Cyan8802_0335

CYC:	PCC7424_1808

CYJ:	Cyan7822_0007

TER:

Tery_3884

ARP:	NIES39_M01550

PAGH:

NIES204_01290

PPSU:

NO713_03545(VTE4)

PRUN:

PCC7821_04187(VTE4)

MVAG:

D0A34_10570

LFS:	HFV01_13475

LIX:	H2674_12695

OXY:	HCG48_01380

OAC:	Oscil6304_3680

PHOR:

JWS08_01935

PYH:	NEA10_11615

ONI:	Osc7112_4375

MIC:	Mic7113_5161

MPRO:

BJP34_07905

GEI:	GEI7407_0067

GVI:

glr3090

GLJ:	GKIL_0804(ubiE)

GML:	ISF26_14310

ANA:

alr1803

NPU:	Npun_R6023

NOS:	Nos7107_1174

NOP:	Nos7524_4363

NON:	NOS3756_12040

NFL:	COO91_06862

NOE:	CLI64_01445

NSH:

GXM_00587

NED:	HUN01_03345

NPZ:	ACX27_23190

NLK:	NIES25_21560

NCW:	NIES2107_67610

NCF:	ANSO36C_40450

NOST:

GJB62_10145

NOSU:

JYQ62_20350

NOSQ:

PQG02_19355

AVA:

Ava_4806

NAZ:

Aazo_3463

ANB:	ANA_C20681

ACY:	Anacy_5288

AWA:	AA650_08025

ANN:	EH233_12140

CSG:	Cylst_2832

RSIN:

B6N60_00407

HBQ:	QI031_08810

NSPH:

BDGGKGIB_04206

AEE:	IM676_14730

CALO:

Cal7507_0600

CALT:

Cal6303_2077

CALH:

IJ00_11010

CALN:

NIES2098_40970

CALS:

NIES3974_45790

RIV:	Riv7116_6708

MDP:	NIES3275_39270

FIS:	FIS3754_01290

DOU:	BMF77_00886

DFS:	HGD76_15830

DHT:	NG743_19520

DCM:	NIES806_35090

DOT:	EZY12_14640

DOZ:	HEP80_01635

DOC:	HEQ26_21880

CCUR:

IAR63_17070

CRK:	L3I90_10975

AFLO:

HEQ12_05000

STOQ:

K2F26_23350

SKV:	NIES73_45540

RCS:	NIES932_21810

OCF:	WJM97_19080

TOQ:	HCG51_14305

TTQ:	NIES37_61190

ALAX:

NIES50_25380

SCYT:

SAMD_20210

SCYS:

NIES4073_76380

BSEN:

DP114_06380

BOX:	DP113_06330

NCN:	BZZ01_25555

CTHE:

Chro_0307

PLP:	Ple7327_2620

SCS:	Sta7437_3159

STAN:

STA3757_20890

DTB:	BH720_033690

» show all

Reference

1 [PMID:24253640]

Authors

Camara B, d'Harlingue A

Title

Demonstration and solubilization of S-adenosylmethionine: gamma-tocopherol methyltransferase from Capsicum chromoplasts.

Journal

Plant Cell Rep 4:31-2 (1985)
DOI:10.1007/BF00285499

Reference

2 [PMID:12492478]

Authors

Koch M, Lemke R, Heise KP, Mock HP

Title

Characterization of gamma-tocopherol methyltransferases from Capsicum annuum L and Arabidopsis thaliana.

Journal

Eur J Biochem 270:84-92 (2003)
DOI:10.1046/j.1432-1033.2003.03364.x

Reference

3 [PMID:22763462]

Authors

Zhang GY, Liu RR, Xu G, Zhang P, Li Y, Tang KX, Liang GH, Liu QQ

Title

Increased alpha-tocotrienol content in seeds of transgenic rice overexpressing Arabidopsis gamma-tocopherol methyltransferase.

Journal

Transgenic Res 22:89-99 (2013)
DOI:10.1007/s11248-012-9630-2

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.95

IUBMB Enzyme Nomenclature:

2.1.1.95

ExPASy - ENZYME nomenclature database:

2.1.1.95

BRENDA, the Enzyme Database:

2.1.1.95

CAS:	84788-82-9

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Gene (979)   
   MGENES (212)   
   RefGene (767)   
Protein sequence (143)   
   UniProt (136)   
   SWISS-PROT (2)   
   RefSeq(pep) (5)   
DNA sequence (145)   
   RefSeq(nuc) (7)   
   GenBank (73)   
   EMBL (65)   
Protein domain (4)   
   InterPro (3)   
   PROSITE(DOC) (1)   
All databases (1271)   

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