KEGG   ENZYME: 2.1.3.1
Entry
EC 2.1.3.1                  Enzyme                                 
Name
methylmalonyl-CoA carboxytransferase;
transcarboxylase;
methylmalonyl coenzyme A carboxyltransferase;
methylmalonyl-CoA transcarboxylase;
oxalacetic transcarboxylase;
methylmalonyl-CoA carboxyltransferase;
(S)-2-methyl-3-oxopropanoyl-CoA:pyruvate carboxyltransferase;
(S)-2-methyl-3-oxopropanoyl-CoA:pyruvate carboxytransferase carboxytransferase [incorrect]
Class
Transferases;
Transferring one-carbon groups;
Carboxy- and carbamoyltransferases
Sysname
(S)-methylmalonyl-CoA:pyruvate carboxytransferase
Reaction(IUBMB)
(S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate [RN:R00930]
Reaction(KEGG)
R00930;
(other) R00353
Substrate
(S)-methylmalonyl-CoA [CPD:C00683];
pyruvate [CPD:C00022]
Product
propanoyl-CoA [CPD:C00100];
oxaloacetate [CPD:C00036]
Comment
A biotinyl-protein, containing cobalt and zinc. The enzyme, described from the bacterium Propionibacterium shermanii, is unique among the biotin-dependent enzymes in that it catalyses carboxyl transfer between two organic molecules, utilizing two separate carboxyltransferase domains. The enzyme is a very large complex, consisting of a hexameric central core of 12S subunits surrounded by six 5S subunit dimers, each connected to the central core by twelve 1.3S biotin carrier subunits.
History
EC 2.1.3.1 created 1961
Pathway
ec00640  Propanoate metabolism
ec01100  Metabolic pathways
Orthology
K03416  methylmalonyl-CoA carboxyltransferase 5S subunit
K17489  methylmalonyl-CoA carboxyltransferase 12S subunit
K17490  methylmalonyl-CoA carboxyltransferase 1.3S subunit
Genes
DDENKI615_02180 KI615_02185 KI615_08785 KI615_08795 KI615_08800
GEMGM21_0316 GM21_0317 GM21_0318 GM21_0319
GBMGbem_0334 Gbem_0335 Gbem_0336 Gbem_0337
GERKP004_00725 KP004_00730
GSUBKP001_14770
GNTKP003_00415
GPLM1B72_20165
PPDPpro_0033 Ppro_0034 Ppro_0035 Ppro_3082 Ppro_3083 Ppro_3084
BCHSJNE38_23625
SSGSelsp_0507
SRISELR_10340
MHGMHY_07420
MYVG155_29450
MFTXA26_59530
MALVMALV_37140
MAUBMAUB_31710
MMUCC1S78_028595
CEFCE0804 CE0805 CE0806 CE0807
CDIDIP0739(pycB) DIP0740(mmdA) DIP0741 DIP0742
CDPCD241_0674(pycB) CD241_0675(accD2) CD241_0676 CD241_0677
CDHCDB402_0651(pycB) CDB402_0652(accD2) CDB402_0653 CDB402_0654
CDTCDHC01_0674(pycB) CDHC01_0675(accD2) CDHC01_0676 CDHC01_0677
CDECDHC02_0677(pycB) CDHC02_0678(accD2) CDHC02_0679 CDHC02_0680
CDRCDHC03_0660(pycB) CDHC03_0661(accD2) CDHC03_0662 CDHC03_0663
CDACDHC04_0666(pycB) CDHC04_0667(accD2) CDHC04_0668 CDHC04_0669
CDZCD31A_0770(pycB) CD31A_0771(accD2) CD31A_0772 CD31A_0773
CDBCDBH8_0728(pycB) CDBH8_0729(accD2) CDBH8_0730 CDBH8_0731
CDSCDC7B_0689(pycB) CDC7B_0690(accD2) CDC7B_0691 CDC7B_0692
CDDCDCE8392_0687(pycB) CDCE8392_0688(accD2) CDCE8392_0689 CDCE8392_0690
CDWCDPW8_0743(pycB) CDPW8_0744(accD2) CDPW8_0745 CDPW8_0746
CDVCDVA01_0621(pycB) CDVA01_0622(accD2) CDVA01_0623 CDVA01_0624
CDIPERS451417_00670(pycB) ERS451417_00671(accD2) ERS451417_00672 ERS451417_00673
CKPckrop_0042(oadA) ckrop_0043 ckrop_0044 ckrop_0045
CPUCPFRC_02860 CPFRC_02865 CPFRC_02870 CPFRC_02875
CPLCp3995_0577 Cp3995_0578 Cp3995_0579 Cp3995_0580
CPGCP316_02970 CP316_02975 CP316_02980 CP316_02985
CPPCpP54B96_0578 CpP54B96_0579 CpP54B96_0580 CpP54B96_0581
CPKCP1002_02860 CP1002_02865 CP1002_02870 CP1002_02875
CPQCPC231_02860 CPC231_02865 CPC231_02870 CPC231_02875
CPXCPI19_02860 CPI19_02865 CPI19_02870 CPI19_02875
CPZCpPAT10_0570 CpPAT10_0571 CpPAT10_0572 CpPAT10_0573
CORCp267_0594 Cp267_0595 Cp267_0596 Cp267_0597
COPCP31_03200 CP31_03205 CP31_03210 CP31_03215
CODCp106_0552 Cp106_0553 Cp106_0554 Cp106_0555
COSCp4202_0563 Cp4202_0564 Cp4202_0565 Cp4202_0566
COICPCIP5297_02985 CPCIP5297_02990 CPCIP5297_02995 CPCIP5297_03000
COECP258_02985 CP258_02990 CP258_02995 CP258_03000
COUCP162_07510 CP162_07515 CP162_07520 CP162_07525
CPSECPTA_01118 CPTA_01119 CPTA_01120 CPTA_01121
CPSUCPTB_00411 CPTB_00412 CPTB_00413 CPTB_00414
CPSFCPTC_00070 CPTC_00071 CPTC_00072 CPTC_00073
CULCULC22_00619(oadA) CULC22_00620(mmdA) CULC22_00621 CULC22_00622
CUCCULC809_00611(oadA) CULC809_00612(mmdA) CULC809_00613 CULC809_00614
CUECULC0102_0722(oadA) CULC0102_0723(mmdA) CULC0102_0724 CULC0102_0725
CUNCul210932_0641 Cul210932_0642(mmdA) Cul210932_0643 Cul210932_0644
CUSCulFRC11_0614 CulFRC11_0615(mmdA) CulFRC11_0616 CulFRC11_0617
CUQCul210931_0615 Cul210931_0616(mmdA) Cul210931_0617 Cul210931_0618
CUZCul05146_0657 Cul05146_0658(mmdA) Cul05146_0659 Cul05146_0660
CUJCUL131002_0619 CUL131002_0620(mmdA) CUL131002_0621 CUL131002_0622
CAZCARG_02100 CARG_02105 CARG_02110 CARG_02115
CVTB843_03830 B843_03835 B843_03840 B843_03845
COADR71_253 DR71_254 DR71_255 DR71_256
CKUUL82_08890 UL82_08895 UL82_08900 UL82_08905
CMVCMUST_04215 CMUST_04220 CMUST_04225 CMUST_04230
CEICEPID_03475 CEPID_03480 CEPID_03485 CEPID_03490
CLWCLAC_07680 CLAC_07685 CLAC_07690 CLAC_07695
CAQUCAQU_03350 CAQU_03355 CAQU_03360 CAQU_03365
CPEGCPELA_08135 CPELA_08140 CPELA_08145 CPELA_08150
CGKCGERO_02945 CGERO_02950 CGERO_02955 CGERO_02960
CRFFRC0190_00731 FRC0190_00732 FRC0190_00733 FRC0190_00734
CCHOCCHOA_02710 CCHOA_02715 CCHOA_02720 CCHOA_02725
CPSOCPPEL_08365 CPPEL_08370 CPPEL_08375 CPPEL_08380
CMATHBA49_10305 HBA49_10310 HBA49_10315 HBA49_10320
CAMCI6I65_03615 I6I65_03620 I6I65_03625 I6I65_03630
CGLUI6J20_06300 I6J20_06305 I6J20_06310 I6J20_06315
CSILCBE74_03325 CBE74_03330 CBE74_03335 CBE74_03340
CKWCKALI_08895 CKALI_08900 CKALI_08905 CKALI_08910
RHODAOT96_33450
ASUFMNQ99_17180
ARSADJ73_02365 ADJ73_02370 ADJ73_02375 ADJ73_02380
DCOSAMEA4475696_1332 SAMEA4475696_1333 SAMEA4475696_1334 SAMEA4475696_1335
AUSIPK37_14725 IPK37_14730 IPK37_14735 IPK37_14740
PACPPA2005 PPA2006 PPA2007 PPA2008
PAKHMPREF0675_5070 HMPREF0675_5071 HMPREF0675_5072 HMPREF0675_5073
PAVTIA2EST22_09810 TIA2EST22_09815 TIA2EST22_09820 TIA2EST22_09825
PAXTIA2EST36_09790 TIA2EST36_09795 TIA2EST36_09800 TIA2EST36_09805
PAZTIA2EST2_09750 TIA2EST2_09755 TIA2EST2_09760 TIA2EST2_09765
PAWPAZ_c20940(mmdC) PAZ_c20950 PAZ_c20960 PAZ_c20970
PADTIIST44_02805 TIIST44_02810 TIIST44_02815 TIIST44_02820
PCNTIB1ST10_10205 TIB1ST10_10210 TIB1ST10_10215 TIB1ST10_10220
PACCPAC1_10235 PAC1_10240 PAC1_10245 PAC1_10250
PACHPAGK_1918 PAGK_1919 PAGK_1920 PAGK_1921
PACNTIA1EST1_09895 TIA1EST1_09900 TIA1EST1_09905 TIA1EST1_09910
CACNRN83_10340 RN83_10345 RN83_10350 RN83_10355
PRAPALO_00960 PALO_00965 PALO_00970 PALO_00975
PRLBCB70_08380 BCB70_08385 BCB70_08390 BCB70_08395
CGRN4412665_00693 4412665_00694 4412665_00695 4412665_00696
PFRPFREUD_18840(bccp) PFREUD_18850 PFREUD_18860(mmdA) PFREUD_18870
PFRERM25_1792 RM25_1793 RM25_1794 RM25_1795
PACDEGX94_04685 EGX94_04690 EGX94_04695 EGX94_04700
PAUSNCTC13651_02414 NCTC13651_02415 NCTC13651_02416 NCTC13651_02417
PPCHMPREF9154_0665 HMPREF9154_0667 HMPREF9154_0669
ARUBJ5A65_11605 J5A65_11610 J5A65_11615 J5A65_11620
PBOPACID_07970 PACID_07980 PACID_07990 PACID_08000(bccP)
AACIASQ49_00810 ASQ49_00815 ASQ49_00820 ASQ49_00825
ACIJJS278_02521 JS278_02522 JS278_02523 JS278_02524
AJIC0Z10_10440 C0Z10_10445 C0Z10_10450 C0Z10_10455
TFLRPIT_00055 RPIT_00060 RPIT_11355 RPIT_11360 RPIT_11365 RPIT_11370
TFABW733_11045 BW733_11050 BW733_11055 BW733_11060
TESBW730_11920 BW730_11925 BW730_11930 BW730_11935
TEZBKM78_05350 BKM78_05355 BKM78_05360 BKM78_05365
TDFH9L22_11645 H9L22_11650
TLATLA_TLA_01085 TLA_TLA_01086 TLA_TLA_01087 TLA_TLA_01088
TEJKDB89_02260 KDB89_02265 KDB89_02275
RAINRai3103_10160 Rai3103_10165 Rai3103_10170
PRVG7070_04830 G7070_04835 G7070_04840 G7070_04845
BRTJ4N02_13890 J4N02_13895 J4N02_13900 J4N02_13905
NCANoca_1247 Noca_1248 Noca_1249 Noca_1250
NBEBack2_17330
MGGMPLG2_3743 MPLG2_3744(yqjD) MPLG2_3745 MPLG2_3746
TALXFOF52_14945
SBAEDSM104329_02823
DFCDFI_07755 DFI_07760 DFI_07765 DFI_07770
AMUCPan181_03350 Pan181_03360 Pan181_03370 Pan181_03380
PLHVT85_16875 VT85_16880 VT85_16885 VT85_16890
PBORBSF38_02050 BSF38_02051 BSF38_02052 BSF38_02053
AGVOJF2_03070 OJF2_03080 OJF2_03100
PCORKS4_34690
ABAAcid345_3023 Acid345_3024 Acid345_3025 Acid345_3026
SUSAcid_2261 Acid_6958 Acid_6959 Acid_6960 Acid_6961
PFERIRI77_05250 IRI77_05255 IRI77_05260 IRI77_05265
CLZBIU88_04850 BIU88_04855 BIU88_04860 BIU88_04865
CPHCpha266_1591 Cpha266_1592 Cpha266_1593 Cpha266_1594
 » show all
Reference
1  [PMID:16590594]
  Authors
Swick RW, Wood HG.
  Title
THE ROLE OF TRANSCARBOXYLATION IN PROPIONIC ACID FERMENTATION.
  Journal
Proc Natl Acad Sci U S A 46:28-41 (1960)
DOI:10.1073/pnas.46.1.28
Reference
2  [PMID:3893281]
  Authors
Wood HG, Kumar GK.
  Title
Transcarboxylase: its quaternary structure and the role of the biotinyl subunit in the assembly of the enzyme and in catalysis.
  Journal
Ann N Y Acad Sci 447:1-22 (1985)
DOI:10.1111/j.1749-6632.1985.tb18422.x
Reference
3  [PMID:15188046]
  Authors
Peikert C, Seeger K, Bhat RK, Berger S.
  Title
Determination of the binding specificity of the 12S subunit of the transcarboxylase by saturation transfer difference NMR.
  Journal
Org Biomol Chem 2:1777-81 (2004)
DOI:10.1039/b404238g
Reference
4  [PMID:17134979]
  Authors
Kumar Bhat R, Berger S.
  Title
New and easy strategy for cloning, expression, purification, and characterization of the 5S subunit of transcarboxylase from Propionibacterium f. shermanii.
  Journal
Prep Biochem Biotechnol 37:13-26 (2007)
DOI:10.1080/10826060601039394
Reference
5  [PMID:15814455]
  Authors
Carey PR, Sonnichsen FD, Yee VC.
  Title
Transcarboxylase: one of nature's early nanomachines.
  Journal
IUBMB Life 56:575-83 (2004)
DOI:10.1080/15216540400022417
Other DBs
ExplorEnz - The Enzyme Database: 2.1.3.1
IUBMB Enzyme Nomenclature: 2.1.3.1
ExPASy - ENZYME nomenclature database: 2.1.3.1
UM-BBD (Biocatalysis/Biodegradation Database): 2.1.3.1
BRENDA, the Enzyme Database: 2.1.3.1
CAS: 9029-86-1

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