KEGG   ENZYME: 2.1.3.12Help
Entry
EC 2.1.3.12                 Enzyme                                 

Name
decarbamoylnovobiocin carbamoyltransferase;
novN (gene name)
Class
Transferases;
Transferring one-carbon groups;
Carboxy- and carbamoyltransferases
BRITE hierarchy
Sysname
carbamoyl phosphate:decarbamoylnovobiocin 3''-O-carbamoyltransferase
Reaction(IUBMB)
carbamoyl phosphate + decarbamoylnovobiocin = phosphate + novobiocin [RN:R06772]
Reaction(KEGG)
Substrate
carbamoyl phosphate [CPD:C00169];
decarbamoylnovobiocin [CPD:C12476]
Product
phosphate [CPD:C00009];
novobiocin [CPD:C05080]
Comment
The enzyme catalyses the last step in the biosynthesis of the aminocoumarin antibiotic novobiocin. The reaction is activated by ATP [1].
History
EC 2.1.3.12 created 2013
Pathway
ec00401  Novobiocin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01130  Biosynthesis of antibiotics
Orthology
K12713  decarbamoylnovobiocin carbamoyltransferase
Genes
NGV: CDO52_02210
Taxonomy
Reference
1  [PMID:14694473]
  Authors
Freel Meyers CL, Oberthur M, Xu H, Heide L, Kahne D, Walsh CT
  Title
Characterization of NovP and NovN: completion of novobiocin biosynthesis by sequential tailoring of the noviosyl ring.
  Journal
Angew Chem Int Ed Engl 43:67-70 (2004)
DOI:10.1002/anie.200352626
  Sequence
Reference
2  [PMID:18997325]
  Authors
Gomez Garcia I, Freel Meyers CL, Walsh CT, Lawson DM
  Title
Crystallization and preliminary X-ray analysis of the O-carbamoyltransferase NovN from the novobiocin-biosynthetic cluster of Streptomyces spheroides.
  Journal
Acta Crystallogr Sect F Struct Biol Cryst Commun 64:1000-2 (2008)
DOI:10.1107/S1744309108030145
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.3.12
IUBMB Enzyme Nomenclature: 2.1.3.12
ExPASy - ENZYME nomenclature database: 2.1.3.12
BRENDA, the Enzyme Database: 2.1.3.12

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