KEGG ENZYME: 2.3.1.165

ENZYME: 2.3.1.165

Entry

EC 2.3.1.165                Enzyme

Name

6-methylsalicylic-acid synthase;
MSAS;
6-methylsalicylic acid synthase

Class

Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups

Sysname

acyl-CoA:malonyl-CoA C-acyltransferase (decarboxylating, oxoacyl-reducing, thioester-hydrolysing and cyclizing)

Reaction(IUBMB)

acetyl-CoA + 3 malonyl-CoA + NADPH + H+ = 6-methylsalicylate + 4 CoA + 3 CO2 + NADP+ + H2O [RN:R07253]

Reaction(KEGG)

R07253

Substrate

acetyl-CoA [CPD:C00024];
malonyl-CoA [CPD:C00083];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Product

6-methylsalicylate [CPD:C02657];
CoA [CPD:C00010];
CO2 [CPD:C00011];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Comment

A multienzyme complex with a tightly-bound 4'-phosphopantetheine cofactor on the acyl carrier protein. It has a similar sequence to vertebrate type I fatty acid synthase. Acetoacetyl-CoA can also act as a starter molecule.

History

EC 2.3.1.165 created 2002

Pathway

ec01059

Biosynthesis of enediyne antibiotics

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K15320

6-methylsalicylic acid synthase

Genes

TATV:

25784653(TrAtP1_010563)

ELA:	UCREL1_5691

PFY:	PFICI_12928

GLZ:	GLAREA_12216

ACT:	ACLA_093660

AOR:	AO090206000074

ANG:	An10g00140

AFV:	AFLA_012951

ACHE:

ACHE_30894A

APUU:

APUU_30248A

PCS:	N7525_005421 N7525_011590

PDP:	PDIP_42600

ABE:

ARB_05854

TVE:

TRV_06867

PNO:	SNOG_00477

ARAB:

EKO05_0002750

NPA:	UCRNP2_4356 UCRNP2_6207 UCRNP2_8850

TML:	GSTUM_00011480001

MMC:

Mmcs_3472

MKM:

Mkms_3535

MJL:

Mjls_3485

MYV:	G155_19265

MFJ:	MFLOJ_23440(ppsA)

MSTO:

MSTO_27400

MLW:	MJO58_05505

MNM:	MNVM_03640(ppsA_1)

MGAU:

MGALJ_22330(ppsA_2)

MSPG:

F6B93_15055

MADI:

A7U43_12650

MYW:	BVC93_21310

MYJ:	BCA37_21480

MVA:

Mvan_3852

MGI:

Mflv_2690

MSP:	Mspyr1_21270

MNE:	D174_18820

MYN:	MyAD_18460

MFT:	XA26_39310

MPHL:

MPHLCCUG_03492(ppsA_2)

MVQ:

MYVA_3673

MRH:	MycrhN_4318

MTHN:

4412656_02813(ppsA_3)

MHAS:

MHAS_01350(aziB)

MDU:	MDUV_07090(ppsA)

MCHT:

MCHIJ_31530(ppsA)

MAUU:

NCTC10437_03653(ppsA_4)

MMOR:

MMOR_27500(ppsA)

MALV:

MALV_00750

MTY:	MTOK_59180(ppsA)

MARZ:

MARA_60360(ppsA)

MGAD:

MGAD_01510(ppsA_1)

MHEV:

MHEL_20690(ppsA)

MPOF:

MPOR_40430(ppsA_2)

MBOK:

MBOE_34580(ppsA)

MFG:	K6L26_12495

MSEI:

MSEDJ_48250(ppsA_2)

MFLV:

NCTC10271_01760(ppsA_2)

MCEE:

MCEL_02350(ppsA)

MMON:

EWR22_17970

MHOL:

K3U96_09850

MSEN:

K3U95_16650

MDF:	K0O62_18905

MGRO:

FZ046_23135

MAUS:

JN090_17155

MFRE:

EXE63_25980

MBUG:

MU0053_001753

MYH:	QU592_19995

MPAT:

MPNTM1_03286(ppsA_1)

MJD:	JDM601_1527(ppsD)

MKK:	MU0083_001353

SGRF:

SGFS_036880

SPRI:

SPRI_0139 SPRI_7214

STRT:

A8713_24070

SGM:	GCM10017557_73720(ppsA)

SSIA:

A7J05_07935

SNK:	CP967_12660

SPAD:

DVK44_32350

SROI:

IAG44_01185

SCOA:

QU709_16605

SARM:

DVA86_12585

SCAJ:

O1G22_20285

KIT:	CFP65_1234

NOA:	BKM31_19850

FRE:	Franean1_3611

SEN:	SACE_5308(chlB1)

AOI:	AORI_5397(ppsA)

ACOR:

LCL61_17735 LCL61_18495

ANW:	QP939_27870

AMYE:

MJQ72_33520

PSEH:

XF36_29085

SESP:

BN6_39100(pks6-1)

ACTI:

UA75_18040

ACAD:

UA74_17515

ACTA:

C1701_26450

MCAB:

HXZ27_20980

VMA:	VAB18032_24830

PSUU:

Psuf_000690(ppsA)

CCAZ:

COUCH_15990

» show all

Reference

1 [PMID:1471999]

Authors

Spencer JB, Jordan PM.

Title

Purification and properties of 6-methylsalicylic acid synthase from Penicillium patulum.

Journal

Biochem J 288 ( Pt 3):839-46 (1992)
DOI:10.1042/bj2880839

Reference

2 [PMID:8823160]

Authors

Child CJ, Spencer JB, Bhogal P, Shoolingin-Jordan PM.

Title

Structural similarities between 6-methylsalicylic acid synthase from Penicillium patulum and vertebrate type I fatty acid synthase: evidence from thiol modification studies.

Journal

Biochemistry 35:12267-74 (1996)
DOI:10.1021/bi960422e

Reference

3 [PMID:10935930]

Authors

Richardson MT, Pohl NL, Kealey JT, Khosla C.

Title

Tolerance and specificity of recombinant 6-methylsalicyclic acid synthase.

Journal

Metab Eng 1:180-7 (1999)
DOI:10.1006/mben.1999.0113

Other DBs

ExplorEnz - The Enzyme Database:

2.3.1.165

IUBMB Enzyme Nomenclature:

2.3.1.165

ExPASy - ENZYME nomenclature database:

2.3.1.165

BRENDA, the Enzyme Database:

2.3.1.165

CAS:

9045-37-8

All links

Gene (17)   
   MGENES (4)   
   RefGene (13)   
Protein sequence (296)   
   UniProt (282)   
   SWISS-PROT (8)   
   RefSeq(pep) (6)   
DNA sequence (268)   
   RefSeq(nuc) (210)   
   GenBank (30)   
   EMBL (28)   
Protein domain (22)   
   InterPro (22)   
All databases (603)   

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