KEGG   ENZYME: 2.3.1.165
Entry
EC 2.3.1.165                Enzyme                                 
Name
6-methylsalicylic-acid synthase;
MSAS;
6-methylsalicylic acid synthase
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
acyl-CoA:malonyl-CoA C-acyltransferase (decarboxylating, oxoacyl-reducing, thioester-hydrolysing and cyclizing)
Reaction(IUBMB)
acetyl-CoA + 3 malonyl-CoA + NADPH + H+ = 6-methylsalicylate + 4 CoA + 3 CO2 + NADP+ + H2O [RN:R07253]
Reaction(KEGG)
R07253
Substrate
acetyl-CoA [CPD:C00024];
malonyl-CoA [CPD:C00083];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Product
6-methylsalicylate [CPD:C02657];
CoA [CPD:C00010];
CO2 [CPD:C00011];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A multienzyme complex with a 4'-phosphopantetheine prosthetic group on the acyl carrier protein. It has a similar sequence to vertebrate type I fatty acid synthase. Acetoacetyl-CoA can also act as a starter molecule.
History
EC 2.3.1.165 created 2002
Pathway
ec01059  Biosynthesis of enediyne antibiotics
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K15320  6-methylsalicylic acid synthase
Genes
ELAUCREL1_5691
PFYPFICI_12928
GLZGLAREA_12216
ACTACLA_093660
AORAO090206000074
ANGANI_1_136174(An10g00140)
AFVAFLA_012951
ACHEACHE_30894A
APUUAPUU_30248A
PCSPc16g00370 Pc22g08170
PDPPDIP_42600
ABEARB_05854
TVETRV_06867
PNOSNOG_00477
NPAUCRNP2_4356 UCRNP2_6207 UCRNP2_8850
TMLGSTUM_00011480001
MMCMmcs_3472
MKMMkms_3535
MJLMjls_3485
MYVG155_19265
MFJMFLOJ_23440(ppsA)
MSTOMSTO_27400
MGROFZ046_23135
MNMMNVM_03640(ppsA_1)
MGAUMGALJ_22330(ppsA_2)
MDFK0O62_18905
MHOLK3U96_09850
MSENK3U95_16650
MSPGF6B93_15055
MVAMvan_3852
MGIMflv_2690
MSPMspyr1_21270
MNED174_18820
MYNMyAD_18460
MFTXA26_39310
MPHLMPHLCCUG_03492(ppsA_2)
MVQMYVA_3673
MRHMycrhN_4318
MTHN4412656_02813(ppsA_3)
MHASMHAS_01350(aziB)
MDUMDUV_07090(ppsA)
MCHTMCHIJ_31530(ppsA)
MAUUNCTC10437_03653(ppsA_4)
MMORMMOR_27500(ppsA)
MALVMALV_00750
MTYMTOK_59180(ppsA)
MARZMARA_60360(ppsA)
MGADMGAD_01510(ppsA_1)
MHEVMHEL_20690(ppsA)
MPOFMPOR_40430(ppsA_2)
MBOKMBOE_34580(ppsA)
MFGK6L26_12495
MSEIMSEDJ_48250(ppsA_2)
MFLVNCTC10271_01760(ppsA_2)
MCEEMCEL_02350(ppsA)
MJDJDM601_1527(ppsD)
NADNCTC11293_04860(ppsA_4)
SPRISPRI_0139 SPRI_7214
STRTA8713_24070
SGMGCM10017557_73720(ppsA)
SSIAA7J05_07935
SALJSMD11_6411
SNKCP967_12660
SRKFGW37_30650
SPADDVK44_32350
SMOBJ7W19_25580
SROIIAG44_01185
KITCFP65_1234
NOABKM31_19850 BKM31_21690
NOWGBF35_46480
FREFranean1_3611
SENSACE_5308(chlB1)
AOIAORI_5397(ppsA)
PSEHXF36_29085
SESPBN6_39100(pks6-1)
ACTIUA75_18040
ACADUA74_17515
ACTAC1701_26450
MCABHXZ27_04065(evdD3) HXZ27_20980
VMAVAB18032_24830
PSUUPsuf_000690(ppsA)
CCAZCOUCH_15990
 » show all
Reference
1  [PMID:1471999]
  Authors
Spencer JB, Jordan PM.
  Title
Purification and properties of 6-methylsalicylic acid synthase from Penicillium patulum.
  Journal
Biochem J 288 ( Pt 3):839-46 (1992)
DOI:10.1042/bj2880839
Reference
2  [PMID:8823160]
  Authors
Child CJ, Spencer JB, Bhogal P, Shoolingin-Jordan PM.
  Title
Structural similarities between 6-methylsalicylic acid synthase from Penicillium patulum and vertebrate type I fatty acid synthase: evidence from thiol modification studies.
  Journal
Biochemistry 35:12267-74 (1996)
DOI:10.1021/bi960422e
Reference
3  [PMID:10935930]
  Authors
Richardson MT, Pohl NL, Kealey JT, Khosla C.
  Title
Tolerance and specificity of recombinant 6-methylsalicyclic acid synthase.
  Journal
Metab Eng 1:180-7 (1999)
DOI:10.1006/mben.1999.0113
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.165
IUBMB Enzyme Nomenclature: 2.3.1.165
ExPASy - ENZYME nomenclature database: 2.3.1.165
BRENDA, the Enzyme Database: 2.3.1.165
CAS: 9045-37-8

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