This enzyme exhibits absolute specificity for the 3alpha configuration found in tropine (tropan-3alpha-ol). Pseudotropine (tropan-3beta-ol) is not a substrate [3]. (E)-2-methylcrotonoyl-CoA (tigloyl-CoA) and acetyl-CoA are the best substrates.
Robins RJ, Bachmann P, Robinson T, Rhodes MJ, Yamada Y.
Title
The formation of 3 alpha- and 3 beta-acetoxytropanes by Datura stramonium transformed root cultures involves two acetyl-CoA-dependent acyltransferases.