KEGG   ENZYME: 2.3.1.186Help
Entry
EC 2.3.1.186                Enzyme                                 

Name
pseudotropine acyltransferase;
pseudotropine:acyl-CoA transferase;
tigloyl-CoA:pseudotropine acyltransferase;
acetyl-CoA:pseudotropine acyltransferase;
pseudotropine acetyltransferase;
pseudotropine tigloyltransferase;
PAT
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
acyl-CoA:pseudotropine O-acyltransferase
Reaction(IUBMB)
an acyl-CoA + pseudotropine = CoA + an O-acylpseudotropine [RN:R08713]
Reaction(KEGG)
Substrate
acyl-CoA [CPD:C00040];
pseudotropine [CPD:C02066]
Product
CoA [CPD:C00010];
O-acylpseudotropine [CPD:C17378]
Comment
This enzyme exhibits absolute specificity for the exo/3beta configuration found in pseudotropine as tropine (tropan-3alpha-ol; see EC 2.3.1.185, tropine acyltransferase) and nortropine are not substrates [1]. Acts on a wide range of aliphatic acyl-CoA derivatives, including acetyl-CoA, beta-methylcrotonyl-CoA and tigloyl-CoA [1].
History
EC 2.3.1.186 created 2008
Pathway
ec00960  Tropane, piperidine and pyridine alkaloid biosynthesis
Reference
1
  Authors
Rabot, S., Peerless, A.C.J. and Robins, R.J.
  Title
Tigloyl-CoA:pseudotropine acyltransferase - an enzyme of tropane alkaloid biosynthesis.
  Journal
Phytochemistry 39:315-322 (1995)
Reference
2  [PMID:1959620]
  Authors
Robins RJ, Bachmann P, Robinson T, Rhodes MJ, Yamada Y.
  Title
The formation of 3 alpha- and 3 beta-acetoxytropanes by Datura stramonium transformed root cultures involves two acetyl-CoA-dependent acyltransferases.
  Journal
FEBS Lett 292:293-7 (1991)
DOI:10.1016/0014-5793(91)80887-9
Reference
3
  Authors
Robins, R.J., Bachmann,P., Peerless, A.C.J. and Rabot, S.
  Title
Esterification reactions in the biosynthesis of tropane alkaloids in transformed root cultures.
  Journal
Plant Cell, Tissue Organ Cult 38:241-247 (1994)
Reference
4  [PMID:10626376]
  Authors
Boswell HD, Drager B, McLauchlan WR, Portsteffen A, Robins DJ, Robins RJ, Walton NJ.
  Title
Specificities of the enzymes of N-alkyltropane biosynthesis in Brugmansia and Datura.
  Journal
Phytochemistry 52:871-8 (1999)
DOI:10.1016/S0031-9422(99)00293-9
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.186
IUBMB Enzyme Nomenclature: 2.3.1.186
ExPASy - ENZYME nomenclature database: 2.3.1.186
BRENDA, the Enzyme Database: 2.3.1.186
CAS: 138440-78-5 162535-26-4

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