Entry
Name
tRNAMet cytidine acetyltransferase;
YpfI;
TmcA
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
acetyl-CoA:[elongator tRNAMet]-cytidine34 N4-acetyltransferase (ATP-hydrolysing)
Reaction(IUBMB)
[elongator tRNAMet]-cytidine34 + ATP + acetyl-CoA + H2O = CoA + [elongator tRNAMet]-N4-acetylcytidine34 + ADP + phosphate
Substrate
[elongator tRNAMet]-cytidine34;
ATP [CPD:
C00002 ];
acetyl-CoA [CPD:
C00024 ];
H2O [CPD:
C00001 ]
Product
CoA [CPD:
C00010 ];
[elongator tRNAMet]-N4-acetylcytidine34;
ADP [CPD:
C00008 ];
phosphate [CPD:
C00009 ]
Comment
The enzyme acetylates the wobble base cytidine34 of the CAU anticodon of elongation-specific tRNAMet. Escherichia coli TmcA strictly discriminates elongator tRNAMet from tRNAIle, which is structurally similar and has the same anticodon loop, mainly by recognizing the C27-G43 pair in the anticodon stem. The enzyme can use GTP in place of ATP for formation of N4-acetylcytidine [1].
History
EC 2.3.1.193 created 2011
Orthology
K06957 tRNA(Met) cytidine acetyltransferase
Genes
ECOO : ECRM13514_3295(ypfI)
ECOH : ECRM13516_3153(ypfI)
EDJ : ECDH1ME8569_2400(ypfI)
ECOI : ECOPMV1_02666(tmcA)
REE : electrica_01329(tmcA)
RTG : NCTC13098_01815(tmcA_2)
CLAP : NCTC11466_01142(tmcA)
KIE : NCTC12125_00251(tmcA)
AHN : NCTC12129_01351(tmcA)
METY : MRY16398_14430(tmcA)
PSHI : SAMEA2665130_0695(tmcA)
SMW : SMWW4_v1c36160(tmcA)
SFJ : SAMEA4384070_3629(tmcA)
SOF : NCTC11214_03159(tmcA_1)
TPTY : NCTC11468_02722(tmcA)
PHEI : NCTC12003_01819(tmcA)
PRJ : NCTC6933_02107(tmcA)
LRI : NCTC12151_00987(tmcA)
HIC : NTHIC486_01403(tmcA)
HPIT : NCTC13334_01242(tmcA)
HHZ : NCTC10839_01679(tmcA)
HAEG : NCTC8502_01779(tmcA)
PDAG : 4362423_01314(ypfI)
ADP : NCTC12871_00610(tmcA)
ALIG : NCTC10568_00739(tmcA)
ASEG : NCTC10977_01931(tmcA)
AVT : NCTC3438_00969(tmcA)
RPNE : NCTC8284_02844(ypfI_1)
PAET : NCTC13378_01326(ypfI)
VQI : CCZ37_13410 CCZ37_18730
VPL : SA104470976_02869(tmcA)
VIB : VspSTUT11_33960(tmcA)
VIS : VspSTUT16_39460(tmcA)
MSHE : MAALD49_36360(tmcA)
SALH : HMF8227_02013(tmcA)
TSE : THMIRHAS_01340(tmcA)
TZO : THMIRHAT_22150(tmcA)
TFRI : Thiofri_02914(tmcA)
HHC : M911_01745 M911_14920
TNI : TVNIR_3636(tmcA_[H])
GAI : IMCC3135_08030(tmcA)
THIC : TspCOW1_30150(tmcA)
HAF : C8233_09950 C8233_09955
HAAH : HALA3H3_590008(tmcA)
AEL : NCTC12917_03724(tmcA)
CHJ : NCTC10426_00146(tmcA)
HJT : DVR14_18000 DVR14_22130
HBU : Hbut_0612 Hbut_0953 Hbut_1153
PFM : Pyrfu_0563 Pyrfu_1302 Pyrfu_1319
PDL : Pyrde_1233 Pyrde_1684 Pyrde_1964
PABI : PABY_10800 PABY_16810 PABY_19900
PARE : PYJP_03350 PYJP_04850 PYJP_17760
SOH : D1869_10735 D1869_12920
SSOA : SULA_0985 SULA_1412
SSOL : SULB_0987 SULB_1413
SSOF : SULC_0986 SULC_1411
SSHI : J5U23_00279 J5U23_03047
SCAS : SACC_06380 SACC_31530
SACN : SacN8_00600 SacN8_02105
SACR : SacRon12I_00600 SacRon12I_02100
SACS : SUSAZ_00645 SUSAZ_02165
SIS : LS215_1842 LS215_2309
SIA : M1425_1713 M1425_2146
SIM : M1627_1829 M1627_2226
SIY : YG5714_1830 YG5714_2273
SIN : YN1551_0643 YN1551_1096
SULA : BFU36_03865 BFU36_06000
SULE : GFS03_09620 GFS03_11695
SULO : GFS33_01435 GFS33_03505
SULL : EWF20_12215 EWF20_13980
MEMJ : MJ1HA_0332 MJ1HA_0344
MHK : DFR87_05515 DFR87_05570
MPRU : DFR88_05330 DFR88_05385
MTEN : GWK48_06390 GWK48_06440
MJN : MjAS7_0860 MjAS7_0871
AMAN : B6F84_06360 B6F84_09485
ABRI : DFR85_06260 DFR85_06310
ASUL : DFR86_05415 DFR86_08170
AAMB : D1866_00285 D1866_10380
ACIH : HS5_19020 HS5_24780
SAZO : D1868_08610 D1868_09855
CSTY : KN1_04430 KN1_06860
» show all
Taxonomy
Reference
Authors
Ikeuchi Y, Kitahara K, Suzuki T
Title
The RNA acetyltransferase driven by ATP hydrolysis synthesizes N4-acetylcytidine of tRNA anticodon.
Journal
Sequence
Reference
Authors
Chimnaronk S, Suzuki T, Manita T, Ikeuchi Y, Yao M, Suzuki T, Tanaka I
Title
RNA helicase module in an acetyltransferase that modifies a specific tRNA anticodon.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 2.3.1.193