Entry
Name
tRNAPhe {7-[3-amino-3-(methoxycarbonyl)propyl]wyosine37-N}-methoxycarbonyltransferase;
TYW4 (ambiguous);
tRNA-yW synthesizing enzyme-4 (ambiguous)
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:tRNAPhe {7-[(3S)-3-amino-3-(methoxycarbonyl)propyl]wyosine37-N}-methyltransferase (carbon dioxide-adding)
Reaction(IUBMB)
S-adenosyl-L-methionine + 7-[(3S)-3-amino-3-(methoxycarbonyl)propyl]wyosine37 in tRNAPhe + CO2 = S-adenosyl-L-homocysteine + wybutosine37 in tRNAPhe [RN:
R10587 ]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:
C00019 ];
7-[(3S)-3-amino-3-(methoxycarbonyl)propyl]wyosine37 in tRNAPhe;
CO2 [CPD:
C00011 ]
Product
S-adenosyl-L-homocysteine [CPD:
C00021 ];
wybutosine37 in tRNAPhe
Comment
The enzyme is found only in eukaryotes, where it is involved in the biosynthesis of wybutosine, a hypermodified tricyclic base found at position 37 of certain tRNAs. The modification is important for translational reading-frame maintenance. In some species that produce hydroxywybutosine the enzyme uses 7-[2-hydroxy-3-amino-3-(methoxycarbonyl)propyl]wyosine37 in tRNAPhe as substrate. The enzyme also has the activity of EC
2.1.1.290 , tRNAPhe [7-(3-amino-3-carboxypropyl)wyosine37-O]-methyltransferase [2].
History
EC 2.3.1.231 created 2013
Orthology
K15451 tRNA wybutosine-synthesizing protein 4
Genes
FPG : 101916966(LCMT2) 101920282
FCH : 102050993 102058812(LCMT2)
CTIG : 120301990 120310020(LCMT2)
SANH : 107663870 107698535
MASI : 127447568 127448113
SCLV : 120339912 120339923
RPHI : 132749668 132754010
CSAT : 104754364 104758239 104773526
HSYR : 120136904 120170320
CCAJ : 109789033 109789034 109789037
PAVI : 110748622 110750645
MSYL : 126613285 126629985
PXB : 103944865 103944920 103962260 103962267
CMAX : 111498153 111499281
CMOS : 111460080 111460624
CPEP : 111778066 111807455
SSPL : 121762362 121763251
ECAD : 122599229 122599607
PSOM : 113298017 113318042
TAES : 123087935 123150831 123164070
MFLO : 136520882 136527519
SMO : SELMODRAFT_110772 SELMODRAFT_176547
CGR : 2886671(GVI51_B01331)
NCS : NCAS_0C04370(NCAS0C04370)
NDI : NDAI_0G03760(NDAI0G03760)
TPF : TPHA_0P01530(TPHA0P01530)
TBL : TBLA_0H00180(TBLA0H00180)
TDL : TDEL_0A00340(TDEL0A00340)
KAF : KAFR_0I02910(KAFR0I02910)
KNG : KNAG_0A01180(KNAG0A01180)
LBG : 92208212(LODBEIA_P30160)
CAL : CAALFM_C101150CA(CaO19.3303)
ASAU : 88172264(PUMCH_001198)
YLI : 2912958(YALI2_F00439g)
NTE : NEUTE1DRAFT123268(NEUTE1DRAFT_123268)
SMP : 10804172(SMAC4_04141)
TTT : THITE_2109301 THITE_2124154
RTHE : 98128071(VTJ83DRAFT_6689)
FPOA : FPOAC1_006757(PPM2)
TATV : 25779539(TrAtP1_012559)
TASP : 36609523(TrAFT101_010177)
CDET : 87942992(CDEST_06489)
BFU : BCIN_13g04270(Bcppm2)
PTRR : 6347206(PtrM4_033930)
ADAC : 96083039(ACET3X_002717)
ACAN : ACA1_274030 ACA1_347670
PLAG : 39744335(PADL01_1439700)
PBRS : 92372768(MKS88_004681)
TAN : TA09190 TA09195 TA09200
TOT : TOT_040000768 TOT_040000769
TET : TTHERM_000312878 TTHERM_00312870
LMAT : 92511190(LSCM1_01052)
LOI : 92357466(LSCM4_01481)
LEIS : 94176696(GH5_01078)
LENR : 94168348(CUR178_01062)
LEIN : 94184818(JIQ42_01070)
PHET : 94287418(JKF63_01294)
» show all
Taxonomy
Reference
Authors
Noma A, Kirino Y, Ikeuchi Y, Suzuki T
Title
Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA.
Journal
Sequence
Reference
Authors
Suzuki Y, Noma A, Suzuki T, Ishitani R, Nureki O
Title
Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4.
Journal
Sequence
Reference
Authors
Kato M, Araiso Y, Noma A, Nagao A, Suzuki T, Ishitani R, Nureki O
Title
Crystal structure of a novel JmjC-domain-containing protein, TYW5, involved in tRNA modification.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 2.3.1.231