KEGG ENZYME: 2.3.1.235

ENZYME: 2.3.1.235

Entry

EC 2.3.1.235                Enzyme

Name

tetracenomycin F2 synthase;
TCM PKS

Class

Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups

Sysname

malonyl-CoA:acetate malonyltransferase (tetracenomycin-F2-forming)

Reaction(IUBMB)

10 malonyl-CoA = tetracenomycin F2 + 10 CoA + 10 CO2 + 2 H2O [RN:R10960]

Reaction(KEGG)

R10960;
(other) R06643 R09269

Substrate

malonyl-CoA [CPD:C00083]

Product

tetracenomycin F2 [CPD:C12366];
CoA [CPD:C00010];
CO2 [CPD:C00011];
H2O [CPD:C00001]

Comment

A multi-domain polyketide synthase involved in the synthesis of tetracenomycin in the bacterium Streptomyces glaucescens. It involves a ketosynthase complex (TcmKL), an acyl carrier protein (TcmM), a malonyl CoA:ACP acyltransferase (MAT), and a cyclase (TcmN). A malonyl-CoA molecule is initially bound to the acyl carrier protein and decarboxylated to form an acetyl starter unit. Additional two-carbon units are added from nine more malonyl-CoA molecules.

History

EC 2.3.1.235 created 2014

Pathway

ec01056

Biosynthesis of type II polyketide backbone

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K05551

minimal PKS ketosynthase (KS/KS alpha)

K05552

minimal PKS chain-length factor (CLF/KS beta)

K15886

multifunctional cyclase / dehydratase / O-methyltransferase

Genes

MBD:	MEBOL_003040 MEBOL_003041

BMEI:

Spa11_38550

BALU:

QRY64_01340

BHUI:

LOK74_08075

PDY:	QJQ58_17940

PAUN:

MJA45_09960

BWX:	NQ550_10275

MSAO:

MYCSP_12695 MYCSP_12700

MSAL:

DSM43276_02542 DSM43276_02543

NBR:	O3I_007445 O3I_007450 O3I_015110 O3I_015115

NNO:	NONO_c10800 NONO_c10810

NTP:	CRH09_08670 CRH09_08675

NOZ:	DMB37_03465 DMB37_03470

NAH:	F5544_10730 F5544_10735 F5544_17020 F5544_17025 F5544_31060 F5544_31065

NIE:	KV110_14170 KV110_14175

NTC:	KHQ06_30030 KHQ06_30035

NYA:	LTV02_09610 LTV02_09615

NGP:	LTT66_26670 LTT66_26675

NSPU:

IFM12276_11090 IFM12276_11100

SCO:	SCO5087(actIORF1) SCO5088(actIORF2)

SMA:	SAVERM_2375(pks9-3) SAVERM_2376(pks9-4)

SFI:	SFUL_4002 SFUL_4003

SANL:

KZO11_29480 KZO11_29485 KZO11_31415

SGRF:

SGFS_016110 SGFS_016120

SCYE:

R2B67_24745 R2B67_24750

SSX:	SACTE_5202 SACTE_5203 SACTE_6346 SACTE_6347

SHY:	SHJG_1188 SHJG_1189

SHO:	SHJGH_1023 SHJGH_1024

SFA:	Sfla_5317 Sfla_5318

SBH:	SBI_06844 SBI_06845

SVE:	SVEN_5977 SVEN_5981

SDV:	BN159_2470 BN159_2471 BN159_2514(tcmL) BN159_2515(tcmK)

SALS:

SLNWT_5121 SLNWT_5122

STRP:

F750_1305(whiE-KS) F750_1306(whiE-CLF)

SCI:	B446_13320 B446_13325

SLV:	SLIV_12945 SLIV_12950

SGU:	SGLAU_26355(tcmK) SGLAU_26360(tcmL) SGLAU_26370(tcmN) SGLAU_28975 SGLAU_28980

SVT:	SVTN_18775 SVTN_18780 SVTN_28595 SVTN_28600

STRE:

GZL_p00214 GZL_p00215

SCW:	TU94_31825 TU94_31830

SLD:	T261_0018 T261_0019 T261_6830 T261_6831

SXI:	SXIM_35600 SXIM_35610

STRM:

M444_36340

STRC:

AA958_14835 AA958_14840 AA958_30255 AA958_30260

SAMB:

SAM23877_0195(alpB) SAM23877_0196(alpA) SAM23877_0207(alpQ) SAM23877_0208(alpR) SAM23877_7465(alpR) SAM23877_7466(alpQ) SAM23877_7477(alpA) SAM23877_7478(alpB)

SPRI:

SPRI_0266 SPRI_0267 SPRI_0278 SPRI_0280 SPRI_7073 SPRI_7075 SPRI_7086 SPRI_7087

SCZ:	ABE83_16240 ABE83_16245

SCX:	AS200_01090 AS200_01095

SRW:	TUE45_01296 TUE45_01297 TUE45_01569 TUE45_01570

STRF:

ASR50_01170 ASR50_01175 ASR50_34265 ASR50_34270

SPAV:

Spa2297_32720 Spa2297_32725

SGS:	AVL59_46040 AVL59_46045

SGM:	GCM10017557_73750(fabF) GCM10017557_73760

SPHW:

NFX46_12165 NFX46_12170 NFX46_24980 NFX46_24985

SLS:	SLINC_6894 SLINC_6895

SALU:

DC74_6590 DC74_6591

SALL:

SAZ_34270 SAZ_34275

STRD:

NI25_18955 NI25_18960

SVU:	B1H20_09640 B1H20_09645

SPUN:

BFF78_19350 BFF78_19355

SLAU:

SLA_3073 SLA_3074

SALJ:

SMD11_0675(actI1) SMD11_0676(actI2) SMD11_1144(actI2) SMD11_6044(actI1) SMD11_6045(actI2)

SLX:	SLAV_40240 SLAV_40245 SLAV_40510 SLAV_40515

SNZ:	DC008_13175 DC008_13180

SGE:	DWG14_07359 DWG14_07360

SLK:	SLUN_33540 SLUN_33545

SDX:	C4B68_09595 C4B68_09600

SGD:	ELQ87_00815 ELQ87_00820 ELQ87_08170 ELQ87_08175

SCYA:

EJ357_29965 EJ357_29970 EJ357_34775 EJ357_34780

SNQ:	CP978_29745 CP978_29750

STIR:

DDW44_27775 DDW44_27780

SKA:	CP970_02735 CP970_02740 CP970_39675 CP970_39680

SGZ:	C0216_31850 C0216_31855

SNK:	CP967_10255 CP967_10260

SHAW:

CEB94_23570 CEB94_23575

SRK:	FGW37_04255 FGW37_04260 FGW37_16265 FGW37_16270

SFIC:

EIZ62_03845 EIZ62_03850

SGAL:

CP966_29070 CP966_29075

SSPO:

DDQ41_07350 DDQ41_07355

SVR:	CP971_23915 CP971_23920

SFY:	GFH48_20450 GFH48_20455

SAQU:

EJC51_31355 EJC51_31360

SGF:	HEP81_07942 HEP81_07943

SCAV:

CVT27_18210 CVT27_18215

SRIM:

CP984_02375 CP984_02380

SCIN:

CP977_28050 CP977_28055

SPAC:

B1H29_00520 B1H29_00525 B1H29_36535 B1H29_36540

STSU:

B7R87_22035 B7R87_22040

SCHA:

CP983_03645 CP983_03650 CP983_27885 CP983_27890

SGX:	H4W23_11745 H4W23_11750

SCOE:

CP976_32910 CP976_32915

SBAT:

G4Z16_23870 G4Z16_23875

SSPB:

CP982_19000 CP982_19005

SNF:	JYK04_04012 JYK04_04013

SPLA:

CP981_11515 CP981_11520 CP981_34525 CP981_34530

STUI:

GCM10017668_38110(fabF) GCM10017668_38120

SCHF:

IPT68_15425 IPT68_15430

SHUN:

DWB77_05172 DWB77_05173

SHAR:

HUT13_24890 HUT13_24895

SCYG:

S1361_29855 S1361_29860

SFEU:

IM697_37550 IM697_37555

SLIA:

HA039_05715 HA039_05720

SDW:	K7C20_32760 K7C20_32765

SCAL:

I6J39_09855 I6J39_09860

SAUH:

SU9_027635 SU9_027640

SMOB:

J7W19_30835 J7W19_30840

SPEU:

CGZ69_24310 CGZ69_24315

SROI:

IAG44_01160 IAG44_09610 IAG44_09615 IAG44_37955 IAG44_37960 IAG44_41430 IAG44_41435

SXN:	IAG42_35940 IAG42_35945 IAG42_36715 IAG42_36720

SHK:	J2N69_28355 J2N69_28360

SANU:

K7396_04305 K7396_04310

SDD:	D9753_08525 D9753_08530

SACT:

DMT42_11840 DMT42_11845

SMAO:

CAG99_12130 CAG99_12135

SGOB:

test1122_15630 test1122_15635

SINE:

KI385_25170 KI385_25175

SLF:	JEQ17_32810 JEQ17_32815

SDEC:

L3078_20575 L3078_20580

SDUR:

M4V62_10140

SYAN:

NRK68_34875 NRK68_34880

STAA:

LDH80_09580 LDH80_09585

SNIG:

HEK616_47540 HEK616_47550(fabF_4)

SSPN:

LXH13_13940 LXH13_13945

SCIR:

STRCI_006078 STRCI_006079 STRCI_006121 STRCI_006122

SLON:

LGI35_31850 LGI35_31855

SDRZ:

NEH16_30130 NEH16_32490 NEH16_32495

STUD:

STRTU_005094 STRTU_005095

SGK:	PET44_33780

SARG:

HKX69_34105 HKX69_34110

SJN:	RI060_18275 RI060_18280

SCYN:

N8I84_12665 N8I84_12670

SOV:	QZH56_16780 QZH56_16790 QZH56_23785 QZH56_23790

SHAU:

K9S39_40675 K9S39_40680

SKG:	KJK29_16070 KJK29_16075

SLAI:

P8A22_09045

SROC:

RGF97_06495

STTN:

BSL84_24940 BSL84_24945

SINN:

ABB07_18370 ABB07_18375

KBU:	Q4V64_27180 Q4V64_27185

SARM:

DVA86_07640 DVA86_07645

STPB:

QR97_20400 QR97_20405 QR97_38780 QR97_38785

SRPA:

HEP86_12720 HEP86_12725

SMIB:

SMIR_18355 SMIR_18360

SCAJ:

O1G22_43255 O1G22_43260

STRL:

HEP84_26740 HEP84_26745

STSD:

HEP87_27710 HEP87_27715 HEP87_54595 HEP87_54600

SRPZ:

HEP85_21125 HEP85_21130

SCHE:

QRN89_14890 QRN89_14895

SGLY:

P8A20_30985 P8A20_30990 P8A20_33850 P8A20_33855

SCAT:

P8A18_34055 P8A18_34060

STII:

DRB96_06775 DRB96_06780

STIC:

DRB89_06925 DRB89_06930

SABD:

N8I86_35455 N8I86_35460

STRV:

EZV63_29965 EZV63_29970

KAB:	B7C62_31265 B7C62_31270

KAU:	B6264_18490 B6264_18495

KIT:	CFP65_4148 CFP65_4149

KIS:	HUT16_23685 HUT16_23690

KCH:	O1G21_22135 O1G21_22140

YIA:	LO772_34315 LO772_34320

STRH:

GXP74_03140 GXP74_03145

ABRY:

NYE86_15465 NYE86_15470 NYE86_15935 NYE86_15940

AGLA:

OIE69_26820 OIE69_26825

AREV:

RVR_1655 RVR_1656 RVR_2903 RVR_2904 RVR_8893 RVR_8894

RATH:

GSU68_08965

LMOI:

VV02_05815 VV02_05820

TFU:	Tfu_1218 Tfu_1219

TALX:

FOF52_07540 FOF52_07545

TCU:	Tcur_2772 Tcur_2773

AGRA:

AGRA3207_002370 AGRA3207_002371

ACIR:

OG979_01950 OG979_01955 OG979_20965 OG979_20970 OG979_36250 OG979_36255

SPIQ:

OHA34_06340 OHA34_06345

SRO:	Sros_4150 Sros_4151

NOA:	BKM31_22830 BKM31_22870

NCX:	Nocox_12685 Nocox_12790

FRA:	Francci3_4100 Francci3_4101 Francci3_4148

FRI:	FraEuI1c_1317 FraEuI1c_1318

SVI:	Svir_33690 Svir_33700

AMD:	AMED_5434(fabF) AMED_5435(fabF)

AMN:	RAM_27700 RAM_27705

AMM:	AMES_5369(fabF) AMES_5370(fabF)

AMZ:	B737_5369(fabF) B737_5370(fabF)

AORI:

SD37_09325 SD37_09330 SD37_34200 SD37_34300

APRT:

MUY14_22560 MUY14_22565

ACYN:

ORV05_20705

ACOR:

LCL61_29035 LCL61_29070

AMYZ:

HUW46_04698(fabF_2)

PSEQ:

AD006_30015 AD006_30020

PECQ:

AD017_31230 AD017_31235

PHH:	AFB00_31125 AFB00_31130

PSDS:

K1T35_37455 K1T35_37460

SESP:

BN6_54860 BN6_54870

SSYI:

EKG83_16315 EKG83_16320 EKG83_32215 EKG83_32220

SACX:

IOD16_13125

KAL:	KALB_7830 KALB_7831

KUT:	JJ691_15650 JJ691_15660

KPHY:

AOZ06_32110 AOZ06_32115

LED:	BBK82_02450 BBK82_02455

AHM:	TL08_10465 TL08_10470

ACTI:

UA75_11090 UA75_11095

ACAD:

UA74_11000 UA74_11005

AHG:	AHOG_10345 AHOG_10350

ACTA:

C1701_02395 C1701_02400

ALO:	CRK55091 CRK55092

PMAD:

BAY61_07805 BAY61_07810

ACTU:

Actkin_02486 Actkin_02487

CROS:

N8J89_20275 N8J89_20280

STP:	Strop_2223 Strop_2224

MAU:	Micau_4018 Micau_4019

MIL:	ML5_4402 ML5_4403

MICH:

FJK98_03840 FJK98_03845

MSAG:

GCM10017556_44980 GCM10017556_44990(fabF)

VMA:	VAB18032_18170 VAB18032_18175

VER:	HUT12_05335 HUT12_05340

MENO:

Jiend_01490

MCHL:

PVK74_07695

MFEU:

H1D33_23035 H1D33_25695 H1D33_25700

MHAW:

RMN56_24400

MCAY:

PVK37_16755 PVK37_16760 PVK37_21255 PVK37_24375 PVK37_24380

MICF:

QQG74_18955

AMS:	AMIS_50420 AMIS_50430 AMIS_50640 AMIS_50650

ACTN:

L083_3108 L083_3109

AIH:	Aiant_86560(fabF_2) Aiant_86570(fabF_3)

ACTR:

Asp14428_76450(fabF_2) Asp14428_76460(fabF_3)

ACTL:

L3i22_085310 L3i22_085320

PLK:	CIK06_11005 CIK06_11010

CATI:

CS0771_73180(fabF_1) CS0771_73190

DVC:	Dvina_17880 Dvina_17885

DFU:	Dfulv_18390 Dfulv_18395

DROS:

Drose_14695 Drose_14745 Drose_24330 Drose_24335

DAUR:

Daura_28645 Daura_28650 Daura_37850 Daura_37855

DACT:

KZZ52_34425 KZZ52_34430

NHY:	JQS43_06230 JQS43_06235

CCAZ:

COUCH_16055 COUCH_16060

CAI:	Caci_3546 Caci_3547 Caci_6485 Caci_6486

KBB:	ccbrp13_66820(fabF_2) ccbrp13_66830

» show all

Reference

1 [PMID:9609708]

Authors

Bao W, Wendt-Pienkowski E, Hutchinson CR

Title

Reconstitution of the iterative type II polyketide synthase for tetracenomycin F2 biosynthesis.

Journal

Biochemistry 37:8132-8 (1998)
DOI:10.1021/bi980466i

Other DBs

ExplorEnz - The Enzyme Database:

2.3.1.235

IUBMB Enzyme Nomenclature:

2.3.1.235

ExPASy - ENZYME nomenclature database:

2.3.1.235

BRENDA, the Enzyme Database:

2.3.1.235

DBGET integrated database retrieval system