KEGG   ENZYME: 2.3.1.244Help
Entry
EC 2.3.1.244                Enzyme                                 

Name
2-methylbutanoate polyketide synthase;
LovF
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
acyl-CoA:malonyl-CoA C-acyltransferase (2-methylbutanoate-forming)
Reaction(IUBMB)
2 malonyl-CoA + [2-methylbutanoate polyketide synthase] + 2 NADPH + 3 H+ + S-adenosyl-L-methionine = (S)-2-methylbutanoyl-[2-methylbutanoate polyketide synthase] + 2 CoA + 2 CO2 + 2 NADP+ + S-adenosyl-L-homocysteine + H2O [RN:R11445]
Reaction(KEGG)
Substrate
malonyl-CoA [CPD:C00083];
[2-methylbutanoate polyketide synthase];
NADPH [CPD:C00005];
H+ [CPD:C00080];
S-adenosyl-L-methionine [CPD:C00019]
Product
(S)-2-methylbutanoyl-[2-methylbutanoate polyketide synthase];
CoA [CPD:C00010];
CO2 [CPD:C00011];
NADP+ [CPD:C00006];
S-adenosyl-L-homocysteine [CPD:C00021];
H2O [CPD:C00001]
Comment
This polyketide synthase enzyme forms the (S)-2-methylbutanoate side chain during lovastatin biosynthesis by the filamentous fungus Aspergillus terreus. The overall reaction comprises a single condensation reaction followed by alpha-methylation, beta-ketoreduction, dehydration, and alpha,beta-enoyl reduction.
History
EC 2.3.1.244 created 2015, modified 2016
Pathway
ec00999  Biosynthesis of secondary metabolites - unclassified
Orthology
K20984  2-methylbutanoate polyketide synthase
Reference
1  [PMID:10334994]
  Authors
Kennedy J, Auclair K, Kendrew SG, Park C, Vederas JC, Hutchinson CR.
  Title
Modulation of polyketide synthase activity by accessory proteins during lovastatin biosynthesis.
  Journal
Science 284:1368-72 (1999)
DOI:10.1126/science.284.5418.1368
  Sequence
Reference
2  [PMID:21069965]
  Authors
Meehan MJ, Xie X, Zhao X, Xu W, Tang Y, Dorrestein PC
  Title
FT-ICR-MS characterization of intermediates in the biosynthesis of the alpha-methylbutyrate side chain of lovastatin by the 277 kDa polyketide synthase  LovF.
  Journal
Biochemistry 50:287-99 (2011)
DOI:10.1021/bi1014776
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.244
IUBMB Enzyme Nomenclature: 2.3.1.244
ExPASy - ENZYME nomenclature database: 2.3.1.244
BRENDA, the Enzyme Database: 2.3.1.244

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