KEGG ENZYME: 2.3.1.245

ENZYME: 2.3.1.245

Entry

EC 2.3.1.245                Enzyme

Name

3-hydroxy-5-phosphooxypentane-2,4-dione thiolase;
lsrF (gene name);
3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase

Class

Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups

Sysname

acetyl-CoA:glycerone phosphate C-acetyltransferase

Reaction(IUBMB)

glycerone phosphate + acetyl-CoA = 3-hydroxy-2,4-dioxopentyl phosphate + CoA [RN:R11182]

Reaction(KEGG)

R11182

Substrate

glycerone phosphate [CPD:C00111];
acetyl-CoA [CPD:C00024]

Product

3-hydroxy-2,4-dioxopentyl phosphate [CPD:C20960];
CoA [CPD:C00010]

Comment

The enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2.

History

EC 2.3.1.245 created 2015, modified 2021

Orthology

K08321

3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase

Genes

ECO:	b1517(lsrF)

ECJ:	JW1510(lsrF)

ECD:	ECDH10B_1648(lsrF)

EBW:	BWG_1336(lsrF)

ECOK:

ECMDS42_1228(lsrF)

ECOC:

C3026_08770

ECE:	Z2188(yneB)

ECS:	ECs_2124(lsrF)

ECF:	ECH74115_2130(lsrF)

ETW:	ECSP_2002(lsrF)

ELX:	CDCO157_1964

EOI:	ECO111_1913(lsrF)

EOH:	ECO103_1647(lsrF)

ECOO:

ECRM13514_1874(lsrF)

ECOH:

ECRM13516_1882(lsrF)

ESL:

O3K_12895

ESO:

O3O_12740

ESM:

O3M_12855

ECK:	EC55989_1650(lsrF)

EOK:	G2583_1882(lsrF)

ELR:	ECO55CA74_09225

ELH:

ETEC_1587

ECW:	EcE24377A_1717(lsrF)

EUN:	UMNK88_1936

ECOA:

APECO78_11330

ECX:	EcHS_A1599(lsrF)

ECM:	EcSMS35_1655(lsrF)

ECY:

ECSE_1607

ECR:	ECIAI1_1529(lsrF)

EUM:	ECUMN_1785(lsrF)

ECT:	ECIAI39_1780(lsrF)

EOC:	CE10_1706(lsrF)

EBR:	ECB_01474(yneB)

ECOB:

C3029_11955

EBL:	ECD_01474(lsrF)

EBE:	B21_01485(lsrF)

EBD:

ECBD_2125

ELO:	EC042_1650

ECL:	EcolC_2141

EKO:	EKO11_2299

EKF:	KO11_15580(lsrF)

EDH:	EcDH1_2129

EDJ:	ECDH1ME8569_1460(lsrF)

ELW:	ECW_m1646(lsrF)

ELL:	WFL_08060(lsrF)

ELP:	P12B_c1559

EFE:	EFER_1560(lsrF)

ESZ:	FEM44_22690(lsrF)

STY:	STY3792(yneB)

STT:	t3540(yneB)

SEX:	STBHUCCB_37220

SENT:

TY21A_17885

STM:	STM4078(yneB)

SEO:	STM14_4903(yneB)

SEV:	STMMW_40431

SEY:	SL1344_4027(yneB)

SEM:	STMDT12_C42250

SEJ:	STMUK_4063(yneB)

SEB:	STM474_4260(yneB)

SEF:	UMN798_4421(yneB)

SETU:

STU288_20540

SETC:

CFSAN001921_20030

SENR:

STMDT2_39411(yneB)

SEND:

DT104_40871(yneB)

SENI:

CY43_21325

SEEN:

SE451236_00500

SPT:	SPA3921(yneB)

SEK:

SSPA3649

SPQ:	SPAB_05052

SEI:	SPC_4184(yneB)

SEC:	SCH_3967(yneB)

SEH:	SeHA_C4409

SHB:

SU5_0175

SENH:

CFSAN002069_11680

SEEH:

SEEH1578_06655

SEE:	SNSL254_A4407

SENN:

SN31241_46100

SEW:	SeSA_A4294

SEA:	SeAg_B4324

SENS:

Q786_20020

SED:

SeD_A4477

SEG:	SG3342(yneB)

SEL:	SPUL_3585(yneB)

SEGA:

SPUCDC_3571(yneB)

SET:	SEN3868(yneB)

SENA:

AU38_19995

SENO:

AU37_20010

SENV:

AU39_20030

SENQ:

AU40_22335

SENL:

IY59_20490

SENJ:

CFSAN001992_13300

SEEB:

SEEB0189_021955

SEEP:

I137_17225

SENB:

BN855_41540

SENE:

IA1_19840

SENC:

SEET0819_04235

SALZ:

EOS98_21920

SFL:

SF1578

SFX:

S1704

SFV:	SFV_1574(yneB)

SFE:	SFxv_1771(lsrF-1)

SFN:

SFy_2263

SFS:

SFyv_2318

SFT:	NCTC1_01711(yneB)

SDY:	SDY_1623(yneB)

SDZ:	Asd1617_02175

SHQ:	A0259_11630

ENC:

ECL_04478

ENL:	A3UG_19785

ECLG:

EC036_38570

ECLE:

ECNIH2_20265

ECLN:

ECNIH4_03440

ECLI:

ECNIH5_18805

ECLX:

LI66_19510

ECLY:

LI62_21510

ECLZ:

LI64_18650

EHM:	AB284_05860

EXF:	BFV63_19010

ECLA:

ECNIH3_18895

ECLC:

ECR091_18820

EAU:	DI57_21475

EKB:	BFV64_20375

ENO:	ECENHK_19215

EEC:	EcWSU1_03894(lsrF)

ELG:	BH714_20325

ECAN:

CWI88_03010

ERN:	BFV67_19045

ECLS:

LI67_020930

ECHG:

FY206_21375(lsrF)

ESH:	C1N69_19875

EPT:	HWQ17_16550(lsrF)

ENT:	Ent638_3532

EAS:	Entas_3757

ENR:	H650_12045

ENX:	NI40_019300

ENF:	AKI40_0666

LNI:	CWR52_15690

EBG:	FAI37_09820(lsrF)

END:	A4308_03880

ENZ:	G0034_19800(lsrF)

ENS:	HWQ15_04780(lsrF)

ENK:	LOC22_07555(lsrF)

EHU:	D5067_0003200(lsrF)

EMOR:

L6Y89_19085(lsrF)

ENB:	ELK40_19660

EQU:	OM418_19220(lsrF)

EPU:	QVH39_20120(lsrF)

EDY:	F0320_18650(lsrF)

KPN:	KPN_03503(yneB)

KPU:

KP1_4800

KPM:	KPHS_46360

KPP:

A79E_0614

KPH:	KPNIH24_05715

KPZ:	KPNIH27_22135

KPV:	KPNIH29_23030

KPW:	KPNIH30_23355

KPY:	KPNIH31_22540

KPG:	KPNIH32_24205

KPC:	KPNIH10_23085

KPQ:	KPR0928_22605

KPT:	VK055_3965

KPO:	KPN2242_20650

KPR:	KPR_4707(lsrF)

KPJ:

N559_0668

KPI:	D364_18050

KPA:	KPNJ1_00656

KPS:	KPNJ2_00698

KPX:	PMK1_01021(lsrF_1)

KPB:	FH42_13800

KPNE:

KU54_003230

KPNU:

LI86_03235

KPNK:

BN49_0604(lsrF)

KVA:

Kvar_0597

KPE:	KPK_0609(lsrF)

KPK:	A593_07765

KVD:	KR75_05030

KVQ:	SP68_11665

KQU:	AVR78_01360

KQV:	B8P98_03275

KOX:

KOX_03290

KOE:

A225_5106

KOY:	J415_06475

KOM:	HR38_01850

KMI:	VW41_02450

KOK:	KONIH1_25810

KOC:	AB185_10515

EAE:

EAE_03930

EAR:

CCG33206

KLL:	BJF97_25465

KLW:	DA718_03715

KAR:	LGL98_03140(lsrF)

KGR:	JJJ10_03340(lsrF)

KPAS:

LUW96_03185(lsrF)

KLC:	K7H21_03330(lsrF)

KLM:	BWI76_24640

REE:	electrica_00592(lsrF)

KLP:	GUC22_03240(lsrF)

ROR:	RORB6_21840

RON:	TE10_24515

RPLN:

B1209_03110

RAO:	DSD31_03280

RTG:	NCTC13098_00815(lsrF)

CAMA:

F384_16545

CAF:	AL524_06520

CFAR:

CI104_21710

CIR:	C2U53_13405

CTEL:

GBC03_08055(lsrF)

CNT:	JT31_09745

CEM:	LH23_08050

CEN:	LH86_07890

CLAP:

NCTC11466_04016(lsrF)

ESC:	Entcl_0618

KLE:	AO703_18350

KSA:	C813_18610

KOR:	AWR26_03080(lsrF)

KRD:	A3780_21655

KPSE:

IP581_20145(lsrF)

KOO:	O9K67_03280(lsrF)

KOU:	C0557_23045

KCA:	AAEY27_03120(lsrF)

KCY:	RIN60_11165(lsrF)

LAX:	APT61_03135

LEI:	C2U54_01595

LEH:	C3F35_15065

LEE:	DVA44_03130

LER:	GNG29_19880(lsrF)

LEA:	GNG26_19070(lsrF)

LPNU:

KQ929_02575(lsrF)

LEY:	DVA43_21150

LAZ:	A8A57_18275

LEF:	LJPFL01_3634

LEW:	DAI21_07790

BUF:	D8682_18535

BAGE:

BADSM9389_05560

BFT:	MNO13_02860(lsrF)

BSEL:

RHD99_02810(lsrF)

AHN:	NCTC12129_00640(lsrF)

ASUB:

NLZ15_02765(lsrF)

YRE:	HEC60_14930(lsrF)

SGOE:

A8O29_003710(lsrF)

KIN:	AB182_28920

PDZ:	HHA33_05625(lsrF)

METY:

MRY16398_06810(lsrF)

SUPE:

P0H77_00655(lsrF)

TOE:	QMG90_18655(lsrF)

MPHG:

MPHASIOC01_000748(lsrF)

EBC:	C2U52_11810

EBU:	CUC76_18745

YPE:

YPO0408

YPK:

y3773

YPH:

YPC_4195

YPA:

YPA_3877

YPN:

YPN_0278

YPM:	YP_3773(fbaB)

YPP:	YPDSF_3223

YPG:	YpAngola_A0862

YPZ:

YPZ3_0400

YPT:	A1122_03060

YPD:

YPD4_0353

YPX:

YPD8_0354

YPW:

CH59_1453

YPJ:

CH55_2402

YPV:

BZ15_3163

YPL:

CH46_506

YPS:	YPTB0548(yneB)

YPO:

BZ17_2011

YPI:	YpsIP31758_3525

YPY:

YPK_3654

YPB:

YPTS_0572

YPQ:

DJ40_1819

YPU:

BZ21_3928

YPR:

BZ20_1545

YPC:

BZ23_28

YPF:

BZ19_4055

YEN:

YE0524

YEP:	YE105_C0537

YEY:

Y11_37581

YEW:

CH47_2952

YET:

CH48_1074

YEF:	FORC2_3565

YEE:	YE5303_28031

YSI:	BF17_11015

YAL:

AT01_3042

YFR:

AW19_3883

YIN:

CH53_1164

YKR:

CH54_1907

YRO:

CH64_3157

YAK:	ACZ76_11820

YMA:	DA391_19425

YHI:	D5F51_02965

YEL:	LC20_04669

YCA:	F0T03_03235(lsrF)

YMO:	HRD69_08370(lsrF)

YAS:	N0H69_02860(lsrF)

YKI:	HRD70_07580(lsrF)

SFW:	WN53_22860

SFG:	AV650_18690

RAH:	Rahaq_2582

RACE:

JHW33_04275(lsrF)

RAQ:	Rahaq2_2595

RAA:

Q7S_12855

ROX:	BV494_07300

RVC:	J9880_17075(lsrF)

RIU:	I2123_14410(lsrF)

EAME:

GXP68_13165(lsrF)

SERA:

Ser39006_021955

SERQ:

CWC46_21965

PROD:

PCO85_03500(lsrF)

SOD:	Sant_3803(lsrF)

SLIG:

GTU79_26060(lsrF)

KPIE:

N5580_06500(lsrF)

PLU:	plu3147(lsrF)

PLUM:

A4R40_15720

PAY:	PAU_01469(yneB)

PTT:	VY86_00295

PAKH:

B0X70_16215

PLUI:

CE143_16205

XBO:	XBJ1_4104(lsrF)

XBV:	XBW1_0359(lsrF)

XNE:	XNC1_4325(lsrF)

XNM:	XNC2_4174(lsrF)

XDO:	XDD1_3690(lsrF)

XHO:	A9255_16440

XBU:	HGO23_01315(lsrF)

XGR:	QL128_01500(lsrF)

MMK:

MU9_2077

EHO:	A9798_12255

HIP:	CGSHiEE_08225

HIA:	H733_1679(lsrF)

HIC:	NTHIC486_00206(lsrF)

HSO:

HS_0055

HSM:

HSM_1948

PMU:

PM1278

PMV:	PMCN06_1943

PUL:	NT08PM_2187

PMP:

Pmu_19390

PMUL:

DR93_522

PSKY:

A6B44_08110

PATL:

KGI96_03270(lsrF)

PCAI:

K7G93_001867(lsrF)

AAP:	NT05HA_0831

AAT:

D11S_1392

AAO:	ANH9381_1738

AAN:

D7S_00019

AAH:	CF65_02217

AACN:

AANUM_1700

AACT:

ACT75_11355

AVT:	NCTC3438_00374(lsrF)

VAS:	GT360_17435(lsrF)

EMP:	EZMO1_4506(lsrF)

CDIZ:

CEDIAZO_00814(lsrF)

RHZ:	RHPLAN_43670

CBAK:

DA792_14440

TPRO:

Ga0080559_TMP2886

SAGU:

CDO87_22060

RSU:

NHU_00496

RHM:	B5V46_15480

PAMO:

BAR1_07160

RCP:	RCAP_rcc02876 RCAP_rcc03013

PMAS:

NCF86_15930(lsrF)

MGM:

Mmc1_1606

TAMM:

GEAMG1_0848(lsrF)

DOL:

Dole_1897

DESB:

BuS5_03160

DMM:	dnm_003660

DTI:	Desti_0485

SFU:

Sfum_2464

PPHT:

GA004_04405(lsrF)

ROO:	G5S37_14050

LPAV:

PLANPX_2509

LLH:	I41_21800(lsrF)

KST:	KSMBR1_0624(fba_1)

BROC:

IPI25_14150(lsrF)

BPIT:

BPIT_26060

JET:	L3J17_14595(lsrF)

LPRO:

PQO03_04750(lsrF)

TPI:	TREPR_3783

TRM:	JO41_06315

TMED:

DWB79_02670

TSK:	HRI97_08125(lsrF)

SSM:	Spirs_3581

BCEL:

BcellWH2_01197(lsrF)

BIS:	DXK01_020510(lsrF)

BNO:	K8P02_06760(lsrF) K8P02_19830(lsrF)

MICA:

P0L94_15415(lsrF)

PDI:

BDI_0631

PARC:

CI960_07105

PMER:

INE87_00403(lsrF)

PGOL:

K6V26_11705(lsrF)

PJO:	LK449_06480(lsrF)

PFAI:

NXY11_10305(lsrF)

RBC:	BN938_0044

FAX:	FUAX_35260

ATK:	AUTU_49080(lsrF)

CTE:

CT1773

CPC:

Cpar_0433

CLZ:	BIU88_02100

CCH:

Cag_1641

CPH:	Cpha266_0194 Cpha266_2002

CPB:	Cphamn1_2008

CLI:

Clim_1971

PVI:

Cvib_0463

PLT:

Plut_0411

PPH:	Ppha_2335 Ppha_2745

PAA:

Paes_1802

PROC:

Ptc2401_01899(lsrF)

PRS:	B9H02_09055

PROS:

CHL67_09625

PROW:

OO006_02605(lsrF)

PROI:

OO005_02570(lsrF)

BAN:

BA_2982

BAR:

GBAA_2982

BAT:

BAS2771

BAH:	BAMEG_1621

BAI:

BAA_3036

BAX:	H9401_2845

BANT:

A16_30090

BANR:

A16R_30550

BANS:

BAPAT_2860

BANH:

HYU01_14750

BANV:

DJ46_1745

BCE:

BC2967

BCA:

BCE_3019

BCZ:	BCE33L2700(fbaA)

BCR:	BCAH187_A3021

BCB:	BCB4264_A2983

BCU:	BCAH820_2979

BCG:	BCG9842_B2259

BCQ:	BCQ_2803(fbaA)

BCX:

BCA_3053

BAL:	BACI_c29370

BNC:

BCN_2827

BCF:

bcf_14545

BCER:

BCK_20010

BTK:	BT9727_2720(fbaA)

BTL:

BALH_2668

BTB:	BMB171_C2670

BTT:

HD73_3006

BTHR:

YBT1520_14785

BTHI:

BTK_14885

BTC:	CT43_CH2968

BTF:	YBT020_14845

BTM:

MC28_2167

BTG:	BTB_c30960

BTI:

BTG_04500

BTN:	BTF1_12365

BTHT:

H175_ch3019

BTHU:

YBT1518_16400

BTW:

BF38_4135

BTHY:

AQ980_16110

BWE:	BcerKBAB4_2776

BWW:	bwei_2045(lsrF)

BMYO:

BG05_3045

BTY:	Btoyo_0263

BMYC:

DJ92_64

BBY:	CY96_13680

BWD:	CT694_15920

BTRO:

FJR70_04275(lsrF)

BMOB:

MLA2C4_15690

BNT:	GSN03_14370(lsrF)

BPAC:

LMD38_12205(lsrF)

BPAN:

NLJ82_13995(lsrF)

BALU:

QRY64_16965(lsrF)

BARD:

QRY57_14980(lsrF)

CSOA:

LIS82_25950(lsrF)

OCN:	CUC15_15685

VIM:	GWK91_10915(lsrF)

PPSR:

I6J18_09110(lsrF)

NMK:	CHR53_25575

NDT:	L1999_27365(lsrF)

NCM:	QNK12_16645(lsrF)

NNV:	QNH39_14455(lsrF)

MDG:	K8L98_05160(lsrF)

VAO:	FA707_08130(lsrF)

FCT:	NRE15_11660(lsrF)

GAD:	K8O88_08700(lsrF)

CAH:	CAETHG_4004(lsrF)

CPRF:

K7H06_15390(lsrF)

ACOL:

K5I23_11455(lsrF)

LASA:

L9O85_00940(lsrF)

AIM:	PYS61_00860(lsrF)

ANQ:	PYS63_01860(lsrF)

CFEM:

HCR03_10770(lsrF)

ELM:

ELI_1077

EMT:	CPZ25_008810

ELIM:

B2M23_12995

AWI:	CHL1_001462(lsrF)

CBAF:

JS518_10765(lsrF)

LACY:

A4V08_30775

CHYL:

CE91St63_19770

ECLF:

QMP25_16095(lsrF)

LBP:	V1224_14340(lsrF)

ETM:	CE91St48_12490

GFE:	Gferi_19725

GBX:	SANA_11710(lsrF)

PFAC:

PFJ30894_00534(lsrF)

FBS:	N510_001815(lsrF)

PCAT:

Pcatena_00720

STRI:

C7M71_001020

LEIZ:

IT072_18825(lsrF)

MIP:	AXH82_08930

MPAL:

BO218_11910

MOO:	BWL13_02435(lsrF)

MLV:	CVS47_03273(lsrF_2)

MWA:	E4K62_14095(lsrF)

MCAW:

F6J84_12500(lsrF)

MLZ:	F6J85_02870(lsrF) F6J85_13500(lsrF)

MRN:	K8F61_15480(lsrF)

MICS:

C1N74_15145

MARK:

QUC20_15310(lsrF)

MPLN:

VC184_15785(lsrF)

MSUF:

KV394_07575(lsrF)

MVD:	AWU67_00095

ASOI:

MTP13_04785(lsrF)

PSAI:

C3B54_111264

LEU:	Leucomu_13325

LARI:

KI794_12890(lsrF)

AGG:	C1N71_13050

ACIT:

HPK19_06230(lsrF)

OFA:	NF556_16280(lsrF)

PROP:

QQ658_13050(lsrF)

NCA:

Noca_0399

SRO:

Sros_2413

TBI:

Tbis_1197

MHAI:

OHB01_20665(lsrF)

MSAG:

GCM10017556_45150

TPY:	CQ11_07250

TPYO:

X956_00455

DFO:	Dform_00572

DETH:

HX448_04380(lsrF)

OPR:	Ocepr_0365

CPOR:

BED41_12380

PPIO:

CE91St28_10030(lsrF)

MINF:

MESINF_1402(lsrF)

FVA:	FV113G1_19740

IPO:	Ilyop_0449

CSOM:

MKD34_04130(lsrF)

NMR:

Nmar_1078

NIR:	NSED_06280

NKR:	NKOR_06450

NID:	NPIRD3C_1041

NIN:	NADRNF5_0857(lsrF)

NIW:	Nisw_08070

NCL:	C5F47_03100

NOX:	C5F49_06125

NUE:	C5F50_08140

NIP:	NsoK4_02355(lsrF)

NZT:	NZOSNM25_000802

NCT:	NMSP_0543(aroA'_1)

NIC:	DSQ20_06740

NGA:	Ngar_c22370(lsrF)

NVN:	NVIE_008540(lsrF)

NEV:

NTE_02180

TAA:	NMY3_02387(lsrF)

NFN:	NFRAN_3093(lsrF)

NCV:	NCAV_1110(lsrF)

NDV:	NDEV_1169(fba)

CSY:	CENSYa_0624

NBV:

T478_0889

NIU:	DSQ19_06250

NCK:	QVH35_10480(lsrF)

LOKI:

Lokiarch_24430(lsrF_1) Lokiarch_49990(lsrF_2)

» show all

Reference

1 [PMID:19714241]

Authors

Diaz Z, Xavier KB, Miller ST

Title

The crystal structure of the Escherichia coli autoinducer-2 processing protein LsrF.

Journal

PLoS One 4:e6820 (2009)
DOI:10.1371/journal.pone.0006820

Sequence

[eco:b1517]

Reference

2 [PMID:25225400]

Authors

Marques JC, Oh IK, Ly DC, Lamosa P, Ventura MR, Miller ST, Xavier KB

Title

LsrF, a coenzyme A-dependent thiolase, catalyzes the terminal step in processing the quorum sensing signal autoinducer-2.

Journal

Proc Natl Acad Sci U S A 111:14235-40 (2014)
DOI:10.1073/pnas.1408691111

Sequence

[eco:b1517]

Other DBs

ExplorEnz - The Enzyme Database:

2.3.1.245

IUBMB Enzyme Nomenclature:

2.3.1.245

ExPASy - ENZYME nomenclature database:

2.3.1.245

BRENDA, the Enzyme Database:

2.3.1.245

DBGET integrated database retrieval system