KEGG ENZYME: 2.3.1.250

ENZYME: 2.3.1.250

Entry

EC 2.3.1.250                Enzyme

Name

[Wnt protein] O-palmitoleoyl transferase;
porcupine;
PORCN (gene name)

Class

Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups

Sysname

(9Z)-hexadec-9-enoyl-CoA:[Wnt]-L-serine O-hexadecenoyltransferase

Reaction(IUBMB)

(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine = CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine [RN:R11201]

Reaction(KEGG)

R11201

Substrate

(9Z)-hexadec-9-enoyl-CoA [CPD:C21072];
[Wnt]-L-serine [CPD:C21172]

Product

CoA [CPD:C00010];
[Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine [CPD:C21171]

Comment

The enzyme, found in animals, modifies a specific serine residue in Wnt proteins, e.g. Ser209 in human Wnt3a and Ser224 in chicken WNT1 [2,3]. The enzyme can accept C13 to C16 fatty acids in vitro, but only (9Z)-hexadecenoate modification is observed in vivo [1]. cf. EC 3.1.1.98, [Wnt protein]-O-palmitoleoyl-L-serine hydrolase.

History

EC 2.3.1.250 created 2015

Orthology

K00181

O-palmitoleoyl transferase

Genes

HSA:	64840(PORCN)

PTR:	465615(PORCN)

PPS:	100975031(PORCN)

GGO:	101153520(PORCN)

PON:	100447682(PORCN)

PPYG:

129024779(PORCN)

NLE:	100594546(PORCN)

HMH:

116811655

SSYN:

129476238(PORCN)

MCC:	710426(PORCN)

MCF:	102125855(PORCN)

MTHB:

126945573

MNI:	105493894(PORCN)

CSAB:

103231923(PORCN)

CATY:

105590475(PORCN)

PANU:

101004687(PORCN)

TGE:	112615052(PORCN)

MLEU:

105547730(PORCN)

RRO:	104660667(PORCN)

RBB:	108517458(PORCN)

TFN:	117077372(PORCN)

PTEH:

111534356(PORCN)

CANG:

105515021(PORCN)

CJC:	100398110(PORCN)

SBQ:	101049119(PORCN)

CIMI:

108300886(PORCN)

ANAN:

105730438(PORCN)

CSYR:

103250459(PORCN)

MMUR:

105856379(PORCN)

LCAT:

123628352(PORCN)

PCOQ:

105816785(PORCN)

OGA:	100953924(PORCN)

MMU:	53627(Porcn)

MCAL:

110286489(Porcn)

MPAH:

110313314(Porcn)

RNO:	317368(Porcn)

MCOC:

116068943(Porcn)

ANU:	117694367(Porcn)

ASYL:

127675409(Porcn)

MUN:	110565607(Porcn)

CGE:	100765928(Porcn)

MAUA:

101841408(Porcn)

PLEU:

114701312(Porcn)

MORG:

121442545 121442652

MFOT:

126512874

AAMP:

119804796(Porcn)

NGI:	103741637(Porcn)

HGL:	101700667(Porcn)

CPOC:

100729593(Porcn)

CCAN:

109698114(Porcn)

DORD:

105997850(Porcn)

DSP:	122098788(Porcn)

PLOP:

125344006(Porcn)

NCAR:

124971849

MMMA:

107159837(Porcn)

ITI:	101964148(Porcn)

OCU:

100342483

OPI:	101531355(PORCN)

TUP:	102474936(PORCN)

GVR:	103589460(PORCN)

CFA:	480903(PORCN)

CLUD:

112673145(PORCN)

VVP:	112907223(PORCN)

VLG:	121482331(PORCN)

NPO:	129518886(PORCN)

AML:	100473692(PORCN)

UMR:	103669346(PORCN)

UAH:	113265915(PORCN)

UAR:	123787597(PORCN)

ELK:

111150334

LLV:

125091652

MPUF:

101669917(PORCN)

MNP:	132006883(PORCN)

MLK:	131821668(PORCN)

NVS:	122897028(PORCN)

ORO:	101377277(PORCN)

EJU:	114216825(PORCN)

ZCA:	113930376(PORCN)

MLX:	118009997(PORCN)

NSU:	110570946(PORCN)

LWW:	102730963(PORCN)

FCA:	101093667(PORCN)

PYU:	121026162(PORCN)

PCOO:

112870881(PORCN)

PBG:	122477960(PORCN)

PVIV:

125157652(PORCN)

LRUF:

124509703

PTG:	102967780(PORCN)

PPAD:

109248062(PORCN)

PUC:

125931653

AJU:

106989470

HHV:	120221002(PORCN)

BTA:	101905588 540373(PORCN)

BOM:	102273839(PORCN)

BIU:	109555475(PORCN)

BBUB:

102389940 102394073(PORCN)

BBIS:

104996719(PORCN)

CHX:	102177509(PORCN)

OAS:	101112290(PORCN) 105606369

BTAX:

128062889 128069966(PORCN)

CSUM:

138070876(PORCN)

ODA:	120872776 120882086(PORCN)

CCAD:

122420292 122434506(PORCN)

MREE:

136153443(PORCN)

MBEZ:

129535085(PORCN) 129547028

SSC:	100627921(PORCN)

CFR:	102519750(PORCN)

CBAI:

105079386(PORCN)

CDK:	105103314(PORCN)

VPC:	102531973(PORCN)

BACU:

103004140(PORCN)

BMUS:

118884911(CBFB) 118889021(PORCN)

LVE:	103071172(PORCN)

OOR:	101269503(PORCN)

DLE:	111169492(PORCN) 111179658(CBFB)

PCAD:

102976114 102990752

PSIU:

116743988(CBFB) 116747922(PORCN)

NASI:

112391528(CBFB) 112416012(PORCN)

ECB:	100051598(PORCN)

EAI:	106827434(PORCN)

MYB:	102240738(PORCN)

MYD:	102761947(PORCN)

MMYO:

118653961(PORCN)

MLF:	102423004(PORCN)

MDT:	132224902(PORCN)

PKL:	118731770(PORCN)

EFUS:

103301279(PORCN)

MNA:	107541398(PORCN)

DRO:	112322444(PORCN)

SHON:

118998309(PORCN)

AJM:	119059940(PORCN)

PDIC:

114505219(PORCN)

PHAS:

123832748(PORCN)

MMF:	118635768(PORCN)

PPAM:

129065130(PORCN)

HAI:	109374803(PORCN)

RFQ:	117021673(PORCN)

PALE:

102890685(PORCN)

PGIG:

120587108(PORCN)

PVP:	105310018(PORCN)

RAY:	107515035(PORCN)

MJV:	108404728(PORCN)

TOD:	119246179(PORCN)

SARA:

101553356(PORCN)

SFUM:

130036695(PORCN)

SETR:

125999213(PORCN)

LAV:	100654628(PORCN)

TMU:

101349448

ETF:	101644616(PORCN)

DNM:	101435131(PORCN)

MDO:	100018836(PORCN)

GAS:	123254636(PORCN)

SHR:	100930661(PORCN)

AFZ:

127542453

PCW:	110201002(PORCN)

TVP:	118833300(PORCN)

PBRV:

138177160(PORCN)

OAA:	100090726(PORCN)

GGA:	107050792(PORCN)

PCOC:

116241283

MGP:	104915865(PORCN)

CJO:	107307404(PORCN)

ACYG:

136787383(PORCN)

TGU:	115492077(PORCN)

LSR:	110483405(PORCN)

PMOA:

120504132(PORCN)

OTC:	121337227(PORCN)

FAB:	101819915(PORCN)

OMA:	130266121(PORCN)

PHI:	102113345(PORCN)

PMAJ:

107198878(PORCN)

CCW:	120412223(PORCN)

ACOE:

138100418(PORCN)

SHAB:

115602869(PORCN)

AGEN:

126035191

CLV:	102093109(PORCN)

CUCA:

128849699(PORCN)

ASN:	102382175(PORCN)

AMJ:	102566547(PORCN)

PSS:	102461420(PORCN)

CMY:	102938717(PORCN)

CCAY:

125628233(PORCN)

DCC:	119846991(PORCN)

CPIC:

101949555(PORCN)

TST:	117888851(PORCN)

CABI:

116818876(PORCN)

MRV:	120388535(PORCN)

ACS:	100567937(porcn)

ASAO:

132767797(PORCN)

PVT:	110081188(PORCN)

SUND:

121924116(PORCN)

PBI:	103053075(PORCN)

PMUR:

107291924(PORCN)

CTIG:

120306623(PORCN)

TSR:	106543185 106546205(PORCN)

PGUT:

117675425(PORCN)

APRI:

131189918(PORCN)

PTEX:

113443417(PORCN)

NSS:	113417778(PORCN)

VKO:	123024033(PORCN)

PMUA:

114587580(PORCN)

PRAF:

128405042(PORCN)

ZVI:	118075227(PORCN)

HCG:	128346328(PORCN)

GJA:	107120739(PORCN)

STOW:

125429737(PORCN)

EMC:	129346152(PORCN)

XLA:	397828(porcn.L)

XTR:	394996(porcn)

NPR:	108789209(PORCN)

RTEM:

120913901(PORCN)

BBUF:

120977468(PORCN)

BGAR:

122946336(PORCN)

MUO:	115462000 115463453(PORCN)

GSH:	117349751(PORCN)

DRE:	100006275(porcn) 333999(porcnl)

SRX:	107731151 107731263 107745789 107745836

SANH:

107668005 107668076 107695217 107695296

SGH:	107549961 107550497 107585894

CCAR:

109061989 109065659 109095160 109105327

CAUA:

113073436 113076434 113102075 113107221

CGIB:

127963081 127963229 128018710 128018738 128019149

PTET:

122350048(porcnl) 122350760(porcn)

LROH:

127169199(porcn) 127170288(porcnl)

OMC:	131545698(porcnl) 131545732(porcn)

PPRM:

120469045(porcnl) 120483061(porcn)

RKG:	130086196(porcn) 130094689(porcnl)

MAMB:

125279358(porcn) 125279607(porcnl)

CIDE:

127517565 127518169

CERY:

137026349(porcn) 137027032(porcnl)

MASI:

127414821 127423791 127424656

TROS:

130567714(porcnl) 130568256(porcn)

TDW:	130406879(porcn) 130440144(porcnl)

MANU:

129414052(porcnl) 129414202(porcn)

IPU:	108265120(porcn) 108265978(porcnl)

IFU:	128607848(porcnl) 128607956(porcn)

PHYP:

113536477 113542874

SMEO:

124399422(porcn) 124400532(porcnl)

TFD:	113655896(porcnl) 113655968(porcn)

TVC:	132853639(porcn) 132854086(porcnl)

TRN:	134317618(porcnl) 134317684(porcn)

AMEX:

103028991(porcnl) 125806203(porcn)

CMAO:

118812954(porcn) 118813162(porcnl)

EEE:	113581100 113589826

CHAR:

105893660 105898967(porcnl)

TRU:	101068343 101075803(porcn) 115249167

TFS:	130529442 130529943

TNG:	GSTEN00008492G001 GSTEN00023528G001

LCO:	104932129(porcn) 104932803

NCC:	104941482(porcn)

TBEN:

117481496

CGOB:

115010825(porcn) 115021090

PGEO:

117442369 117449932

GACU:

117535475 117546296

EMAC:

134867296 134868583

ELY:	117258359 117258980

EFO:	125891611 125892011

PLEP:

121946928 121947330

SLUC:

116043570 116050880

ECRA:

117947578(porcn) 117948144

ESP:	116692801(porcn) 116693086

PFLV:

114558454 114558943(porcn)

GAT:	120829223 120829918

PPUG:

119222140 119222177 119223717

AFB:	129099465 129099777

CLUM:

117733550 117733641

PSWI:

130199094 130199482

CANA:

137800416 137800992

CVE:	137873411 137889841

MSAM:

119904975

SCHU:

122882377 122882472

CUD:	121516963 121518143

SPUL:

138589860 138589970

ALAT:

119022821 119023139

ASTR:

137605760 137609791

MZE:	101468644(porcn) 101476019

ONL:	100693496 100702239(porcn)

OAU:	116311432 116319534

OLA:	101155172 101170431(porcn)

OML:	112140019 112159828

CSAI:

133440997 133456695

XMA:	102217940 102227294(porcn)

XCO:	114142172 114149109(porcn)

XHE:	116721237 116737533(porcn)

PRET:

103466972 103467706(porcn)

PFOR:

103139050(porcn) 103154223

PLAI:

106954495(porcn) 106957133

PMEI:

106915880(porcn) 106928340

GAF:	122832196 122837021

PPRL:

129370126 129371786

CVG:	107091005 107091484(porcn)

CTUL:

119784972 119786837

GMU:	124870195 124870306

NFU:	107390509(porcn) 107390546

KMR:	108231910 108231935

ALIM:

106519341(porcn) 106527381

NWH:	119416866 119417554

AOCE:

111588285 111588352(porcn)

EJA:	138192101 138193220

BFRE:

138614730 138624279

MCEP:

125015774

CSEM:

103384848 103385376(porcn)

POV:	109630854 109635080(porcn)

SSEN:

122776625

HHIP:

117764921 117765142

HSP:	118110452 118111054

PPLT:

128442241 128443022

SMAU:

118316990 118317438

LCF:

108900495

SDU:	111219005 111235086(porcn)

SLAL:

111654166(porcn) 111670805

XGL:	120804160 120804186

HCQ:	109513006(porcn) 109528448

SSCV:

125988018 125988189

SBIA:

133511818

PEE:	133399582 133408183

PTAO:

133476906 133486495

BPEC:

110156024 110164851(porcn)

MALB:

109966788(porcn)

BSPL:

114859240 114859378

SJO:	128357218 128357919

SSCO:

133987548

SASA:

106566826 106567241 106572297 106572375

STRU:

115165745 115168081 115168256

OTW:	112216182 112221910 112222061

OMY:	110492277 110492779 110532482

OGO:	124032098 124045064 124045924

ONE:	115102223 115102364 115132332

OKI:	109869570 109869636 109908160

OKE:	118359741 118359884 118360844

SALP:

111966642 111977065 111977229

SNH:	120058875 120058959 120060691 120060727

CCLU:

121538206 121538520 121545530

ELS:	105013829 105022369(porcn)

SFM:	108934774(porcn)

PKI:	111847825(porcn)

AANG:

118210576

CCOG:

133110043

LOC:	102684972(porcn)

PSPA:

121328230

ARUT:

117404205

PSEX:

120542054

LCM:	102364055(PORCN)

HOC:

132809909

PMRN:

116949361(PORCN)

LRJ:	133342631(PORCN)

BFO:

118415732

BBEL:

109477032

CIN:

100184764

SCLV:

120347325

SPU:

577959

LPIC:

129256802

APLC:

110988533

ARUN:

117302460

AJC:

117107839

SKO:

100313706

DME:	Dmel_CG6205(por)

DER:

6550117

DSE:

6614853

DSI:	Dsimw501_GD15644(Dsim_GD15644)

DYA:	Dyak_GE15535

DSR:

110175931

DPO:

4814399

DPE:

6601754

DMN:

108162839

DWI:

6641106

DGR:

6569466

DMO:	Dmoj_GI15128

DAZ:

108620003

DNV:

115563932

DHE:

111593036

CCAT:

101448453

BDR:

105228235

AOQ:

129239256

TDA:

119671846

MDE:

101890144

SCAC:

106091266

LCQ:

111682962

LSQ:

119603600

GFS:

119637440

ECOE:

129944727

CLON:

129611769

AGA:

1277989

ACOZ:

120959036

AARA:

120904138

AMER:

121597630

ASTE:

118513906

AFUN:

125766244

AMOU:

128303936

AALI:

118458605

AAG:

5564741

AALB:

109414987 109415134

ASUA:

134215925

CQU:	CpipJ_CPIJ000099

CPII:

120418850

CNS:

116345220

BCOO:

119067887

LLL:

129793725

PPAP:

129801640

AME:

726113

ACER:

108003503

ALAB:

122720734

ADR:

102674318

AFLR:

100871834

BIM:

100740785

BBIF:

117211587

BVK:

117240251

BVAN:

117157118

BHUN:

126869602

BTER:

100648194

BAFF:

126919252

BPYO:

122570759

BPAS:

132910495

FVI:

122529402

OBB:

114879801

OLG:

117600831

MGEN:

117229105

NMEA:

116433156

CGIG:

122399864

SOC:

105193582

MPHA:

105838828

AEC:

105152828

ACEP:

105624957

PBAR:

105424498

VEM:

105566978

HST:

105184742

DQU:

106747608

CFO:

105248892

FEX:

115232774

LHU:	105679336(por)

PGC:

109858919

OBO:

105279498

PCF:

106786776

PFUC:

122522876

VPS:

122630265

VCRB:

124425463

VVE:

124949030

NVI:

100122410

CSOL:

105362089

TPRE:

106659297

LHT:

122510294

LBD:

127283514

MDL:

103574188

CGLO:

123269822

FAS:

105271265

DAM:

107045049

AGIF:

122851534

VCAN:

122414173

CCIN:

107267335

DSM:

124410849

NPT:	124219607(por)

NFB:

124184095

NLO:

107217269

NVG:

124304519

AROA:

105683943

TCA:

659497

DPA:

109541125

SOY:

115882280

AGRG:

126748831

ATD:

109601042

CSET:

123321148

HAXR:

123673584

AGB:

108907390

LDC:

111515703

DVV:

126893221

NVL:	108568744 108568786

APLN:

108739361

PPYR:

116160529

OTU:	111425421 111425422

BMOR:

101737359

BMAN:

114245528

MSEX:

115452376

BANY:

112055417

MJU:

123864139

NIQ:

126775983

VCD:

124539274

MCIX:

123665635

PMAC:

106719603

PPOT:

106107998

PXU:

106117026

BPHI:

137929403(por)

PRAP:

111004230

ZCE:

119829995

LSIN:

126971745

AAGE:

121729795

HAW:

110374464

HZE:

124640324

TNL:

113492516

SLIU:

111354862

OFU:

114364665

ATRA:

106129459

GMW:

116413636

PXY:

105385137

PGW:

126381926

LGLY:

125241904

CCRN:

123302959

API:

100570269

DNX:

107170409

AGS:

114130312

RMD:

113557205

ACOO:

126844941

DVT:

126898431

BTAB:

109032519

DCI:	103514751 103520657

CLEC:

106670556

HHAL:

106678206(por)

NLU:

111048266

HVI:

124369176

MQU:

128998928

PHU:	Phum_PHUM569150

FOC:

113212043

TPAL:

117651741

ZNE:

110831158

CSEC:

111863242

SGRE:

126298607

BROR:

134529451

IEL:

124170835

FCD:

110856733

DPX:	DAPPUDRAFT_303185

DMK:

116924256

DPZ:

124335031

AFRA:

136026977

PVM:

113818886

PJA:

122254583

PCHN:

125028524

PMOO:

119578899

HAME:

121861550

PCLA:

123753984(por)

CQD:	128701575(por)

PTRU:

123504854

ESN:

126996621

MNZ:

135224006

MRJ:	136842697(por)

HAZT:

108672941

EAF:

111701569

LSM:

121127842

PPOI:

119112996 119113011

DSV:

119461453

RSAN:

119396184

RMP:

119174396

VDE:

111250436

VJA:

111258745

TUT:

107363051

DPTE:

113791185

DFR:

124498311

CSCU:

111616324

CVS:	136969197(por)

PTEP:

107439356

ABRU:

129963723

UDV:	129220815 129221351

SDM:

118193395

PMEO:

129581884

CEL:	CELE_T07H6.2(mom-1)

CBR:	CBG_05032(Cbr-mom-1)

CRQ:	GCK72_024553

BMY:	BM_BM5662(Bma-mom-1)

LOA:	LOAG_07740

HRO:	HELRODRAFT_98326

LGI:	LOTGIDRAFT_234449

PVUL:

126828146

PCAN:

112572215

BGT:

106051375

GAE:

121369168

HRF:

124144239

HRJ:

124252948

HASI:

137296644

CRG:

105321139

CANU:

128158895

CVN:

111106534

OED:

125654022

MYI:

110447646

PMAX:

117331615

AIRR:

138336345

MCAF:

127728009

MMER:

128547864

RPHI:

132737663 132747490

DPOL:

127832279

MAEA:

128217537

OBI:

106871820

OSN:

115226104

LJP:

135470124

LAK:

106179299

SMM:	Smp_133180.1

SHX:	MS3_00006239

EGL:

EGR_05657

LLON:

135491500

NVE:

5512182

EPA:

110249538

ATEN:

116297591

AMIL:

114969569

MCAZ:

138015618

MFOO:

138000409

PDAM:

113684790

SPIS:

111327926

DGT:

114531920

XEN:

124445468

HMG:

105848083

HSY:

130655944

RES:

135686010

TAD:	TRIADDRAFT_56916

AQU:

100642144

» show all

Reference

1 [PMID:17141155]

Authors

Takada R, Satomi Y, Kurata T, Ueno N, Norioka S, Kondoh H, Takao T, Takada S

Title

Monounsaturated fatty acid modification of Wnt protein: its role in Wnt secretion.

Journal

Dev Cell 11:791-801 (2006)
DOI:10.1016/j.devcel.2006.10.003

Sequence

[mmu:53627]

Reference

2 [PMID:24292069]

Authors

Gao X, Hannoush RN

Title

Single-cell imaging of Wnt palmitoylation by the acyltransferase porcupine.

Journal

Nat Chem Biol 10:61-8 (2014)
DOI:10.1038/nchembio.1392

Sequence

[hsa:64840]

Reference

3 [PMID:25451226]

Authors

Miranda M, Galli LM, Enriquez M, Szabo LA, Gao X, Hannoush RN, Burrus LW

Title

Identification of the WNT1 residues required for palmitoylation by Porcupine.

Journal

FEBS Lett 588:4815-24 (2014)
DOI:10.1016/j.febslet.2014.11.016

Sequence

[hsa:64840]

Other DBs

ExplorEnz - The Enzyme Database:

2.3.1.250

IUBMB Enzyme Nomenclature:

2.3.1.250

ExPASy - ENZYME nomenclature database:

2.3.1.250

BRENDA, the Enzyme Database:

2.3.1.250

DBGET integrated database retrieval system