Entry |
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Name |
[Wnt protein] O-palmitoleoyl transferase;
porcupine;
PORCN (gene name)
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Class |
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
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Sysname |
(9Z)-hexadec-9-enoyl-CoA:[Wnt]-L-serine O-hexadecenoyltransferase
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Reaction(IUBMB) |
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine = CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine [RN: R11201]
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Reaction(KEGG) |
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Substrate |
(9Z)-hexadec-9-enoyl-CoA [CPD: C21072];
[Wnt]-L-serine [CPD: C21172]
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Product |
CoA [CPD: C00010];
[Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine [CPD: C21171]
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Comment |
The enzyme, found in animals, modifies a specific serine residue in Wnt proteins, e.g. Ser209 in human Wnt3a and Ser224 in chicken WNT1 [2,3]. The enzyme can accept C13 to C16 fatty acids in vitro, but only (9Z)-hexadecenoate modification is observed in vivo [1]. cf. EC 3.1.1.98, [Wnt protein]-O-palmitoleoyl-L-serine hydrolase.
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History |
EC 2.3.1.250 created 2015
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Orthology |
K00181 | O-palmitoleoyl transferase |
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Genes |
» show all
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Reference |
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Authors |
Takada R, Satomi Y, Kurata T, Ueno N, Norioka S, Kondoh H, Takao T, Takada S |
Title |
Monounsaturated fatty acid modification of Wnt protein: its role in Wnt secretion. |
Journal |
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Sequence |
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Reference |
|
Authors |
Gao X, Hannoush RN |
Title |
Single-cell imaging of Wnt palmitoylation by the acyltransferase porcupine. |
Journal |
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Sequence |
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Reference |
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Authors |
Miranda M, Galli LM, Enriquez M, Szabo LA, Gao X, Hannoush RN, Burrus LW |
Title |
Identification of the WNT1 residues required for palmitoylation by Porcupine. |
Journal |
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Sequence |
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Other DBs |
ExPASy - ENZYME nomenclature database: | 2.3.1.250 |
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