Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy

(9Z)-hexadec-9-enoyl-CoA:[Wnt]-L-serine O-hexadecenoyltransferase

(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine = CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine [RN:R11201]

R11201
Reaction

(9Z)-hexadec-9-enoyl-CoA [CPD:C21072];
[Wnt]-L-serine [CPD:C21172]

The enzyme, found in animals, modifies a specific serine residue in Wnt proteins, e.g. Ser209 in human Wnt3a and Ser224 in chicken WNT1 [2,3]. The enzyme can accept C13 to C16 fatty acids in vitro, but only (9Z)-hexadecenoate modification is observed in vivo [1]. cf. EC 3.1.1.98, [Wnt protein]-O-palmitoleoyl-L-serine hydrolase.

EC 2.3.1.250 created 2015

1  [PMID:17141155]

Takada R, Satomi Y, Kurata T, Ueno N, Norioka S, Kondoh H, Takao T, Takada S

Monounsaturated fatty acid modification of Wnt protein: its role in Wnt secretion.

Dev Cell 11:791-801 (2006)
DOI:10.1016/j.devcel.2006.10.003

[mmu:53627]

2  [PMID:24292069]

Gao X, Hannoush RN

Single-cell imaging of Wnt palmitoylation by the acyltransferase porcupine.

Nat Chem Biol 10:61-8 (2014)
DOI:10.1038/nchembio.1392

[hsa:64840]

3  [PMID:25451226]

Miranda M, Galli LM, Enriquez M, Szabo LA, Gao X, Hannoush RN, Burrus LW

Identification of the WNT1 residues required for palmitoylation by Porcupine.

FEBS Lett 588:4815-24 (2014)
DOI:10.1016/j.febslet.2014.11.016

[hsa:64840]