KEGG   ENZYME: 2.3.1.252Help
Entry
EC 2.3.1.252                Enzyme                                 

Name
mycolipanoate synthase;
msl3 (gene name);
Pks3/4;
mycolipanoic acid synthase;
long-chain acyl-CoA:methylmalonyl-CoA C-acyltransferase (mycolipanoate-forming)
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
long-chain acyl-CoA:(S)-methylmalonyl-CoA C-acyltransferase (mycolipanoate-forming)
Reaction(IUBMB)
a long-chain acyl-CoA + 3 (S)-methylmalonyl-CoA + 6 NADPH + 6 H+ + holo-[mycolipanoate synthase] = mycolipanoyl-[mycolipanoate synthase] + 4 CoA + 3 CO2 + 6 NADP+ + 3 H2O
Reaction(KEGG)
(other) R05189 R11451
Show
Substrate
long-chain acyl-CoA [CPD:C02843];
(S)-methylmalonyl-CoA [CPD:C00683];
NADPH [CPD:C00005];
H+ [CPD:C00080];
holo-[mycolipanoate synthase]
Product
mycolipanoyl-[mycolipanoate synthase];
CoA [CPD:C00010];
CO2 [CPD:C00011];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
This mycobacterial enzyme accepts long-chain fatty acyl groups from their CoA esters and extends them by incorporation of three methylmalonyl (but not malonyl) residues, forming trimethyl-branched fatty-acids such as (2S,4S,6S)-2,4,6-trimethyltetracosanoate (C27-mycolipanoate). Since the enzyme lacks a thioesterase domain, the product remains bound to the enzyme and requires additional enzyme(s) for removal.
History
EC 2.3.1.252 created 2016, modified 2019
Orthology
K12432  mycolipanoate synthase
Genes
MTU: Rv1180(pks3) Rv1181(pks4)
MTV: RVBD_1181
MTC: MT1218(mas-1)
MRA: MRA_1191(pks3)
MTF: TBFG_11205
MTB: TBMG_02801
MTK: TBSG_02815
MTZ: TBXG_002781
MTE: CCDC5079_1092
MTUR: CFBS_1259(pks3)
MTL: CCDC5180_1085
MTO: MTCTRI2_1212
MTD: UDA_1180(pks3) UDA_1181(pks4)
MTN: ERDMAN_1323(pks4)
MTJ: J112_06380
MTUB: MT7199_1210(pks3_4)
MTUC: J113_08285
MTUE: J114_06380
MTX: M943_06185
MTUL: TBHG_01164
MBO: BQ2027_MB1213(pks3)
MBB: BCG_1243(pks3)
MBT: JTY_1216(pks3)
MBM: BCGMEX_1215(pks3)
MBK: K60_012730
MAF: MAF_11990(pks3) MAF_12000(pks4)
MCE: MCAN_11921(pks4)
MCQ: BN44_11320(pks)
MCV: BN43_30248(pks)
MCX: BN42_21046(pks)
MCZ: BN45_30243(pks)
MLE: ML1229(pks3)
MLB: MLBr01229(pks3)
MPA: MAP_2603c MAP_2604c
MAO: MAP4_1214
MAV: MAV_1321
MIT: OCO_12290
MIA: OCU_12250
MID: MIP_01963
MYO: OEM_12440
MIR: OCQ_12310
MMM: W7S_06000
 » show all
Taxonomy
Reference
1  [PMID:11278910]
  Authors
Sirakova TD, Thirumala AK, Dubey VS, Sprecher H, Kolattukudy PE
  Title
The Mycobacterium tuberculosis pks2 gene encodes the synthase for the hepta- and  octamethyl-branched fatty acids required for sulfolipid synthesis.
  Journal
J Biol Chem 276:16833-9 (2001)
DOI:10.1074/jbc.M011468200
Reference
2  [PMID:12207710]
  Authors
Dubey VS, Sirakova TD, Kolattukudy PE
  Title
Disruption of msl3 abolishes the synthesis of mycolipanoic and mycolipenic acids  required for polyacyltrehalose synthesis in Mycobacterium tuberculosis H37Rv and  causes cell aggregation.
  Journal
Mol Microbiol 45:1451-9 (2002)
DOI:10.1046/j.1365-2958.2002.03119.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.252
IUBMB Enzyme Nomenclature: 2.3.1.252
ExPASy - ENZYME nomenclature database: 2.3.1.252
BRENDA, the Enzyme Database: 2.3.1.252

  All links  
Chemical substance (12)   
   KEGG COMPOUND (12)   
Chemical reaction (4)   
   KEGG REACTION (2)   
   KEGG RCLASS (2)   
Gene (47)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (46)   
Protein sequence (5)   
   UniProt (4)   
   SWISS-PROT (1)   
DNA sequence (2)   
   GenBank (1)   
   EMBL (1)   
All databases (70)   

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