Entry |
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Name |
mycolipanoate synthase;
msl3 (gene name);
Pks3/4;
mycolipanoic acid synthase;
long-chain acyl-CoA:methylmalonyl-CoA C-acyltransferase (mycolipanoate-forming)
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Class |
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
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Sysname |
long-chain acyl-CoA:(S)-methylmalonyl-CoA C-acyltransferase (mycolipanoate-forming)
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Reaction(IUBMB) |
a long-chain acyl-CoA + 3 (S)-methylmalonyl-CoA + 6 NADPH + 6 H+ + holo-[mycolipanoate synthase] = mycolipanoyl-[mycolipanoate synthase] + 4 CoA + 3 CO2 + 6 NADP+ + 3 H2O
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Reaction(KEGG) |
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Substrate |
long-chain acyl-CoA [CPD: C02843];
(S)-methylmalonyl-CoA [CPD: C00683];
NADPH [CPD: C00005];
H+ [CPD: C00080];
holo-[mycolipanoate synthase]
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Product |
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Comment |
This mycobacterial enzyme accepts long-chain fatty acyl groups from their CoA esters and extends them by incorporation of three methylmalonyl (but not malonyl) residues, forming trimethyl-branched fatty-acids such as (2S,4S,6S)-2,4,6-trimethyltetracosanoate (C27-mycolipanoate). Since the enzyme lacks a thioesterase domain, the product remains bound to the enzyme and requires additional enzyme(s) for removal.
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History |
EC 2.3.1.252 created 2016, modified 2019
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Orthology |
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Genes |
» show all
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Reference |
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Authors |
Sirakova TD, Thirumala AK, Dubey VS, Sprecher H, Kolattukudy PE |
Title |
The Mycobacterium tuberculosis pks2 gene encodes the synthase for the hepta- and octamethyl-branched fatty acids required for sulfolipid synthesis. |
Journal |
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Reference |
|
Authors |
Dubey VS, Sirakova TD, Kolattukudy PE |
Title |
Disruption of msl3 abolishes the synthesis of mycolipanoic and mycolipenic acids required for polyacyltrehalose synthesis in Mycobacterium tuberculosis H37Rv and causes cell aggregation. |
Journal |
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Sequence |
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Other DBs |
ExPASy - ENZYME nomenclature database: | 2.3.1.252 |
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