KEGG   ENZYME: 2.3.1.253Help
Entry
EC 2.3.1.253                Enzyme                                 

Name
phloroglucinol synthase;
phlD (gene name)
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
malonyl-CoA:malonyl-CoA malonyltransferase (decarboxylating, phloroglucinol-forming)
Reaction(IUBMB)
3 malonyl-CoA = phloroglucinol + 3 CO2 + 3 CoA [RN:R10967]
Reaction(KEGG)
Substrate
malonyl-CoA [CPD:C00083]
Product
phloroglucinol [CPD:C02183];
CO2 [CPD:C00011];
CoA [CPD:C00010]
Comment
The enzyme, characterized from the bacterium Pseudomonas protegens Pf-5, is a type III polyketide synthase. The mechanism involves the cyclization of an activated 3,5-dioxoheptanedioate intermediate. The enzyme exhibits broad substrate specificity, and can accept C4-C12 aliphatic acyl-CoAs and phenylacetyl-CoA as the starter molecules, forming 6-(polyoxoalkyl)-alpha-pyrones by sequential condensation with malonyl-CoA.
History
EC 2.3.1.253 created 2016
Orthology
K15431  phloroglucinol synthase
Genes
VNI: VIBNI_A2441(phlD)
VCY: IX92_07225
VCT: JV59_31265
PAG: PLES_25771
PAF: PAM18_2382
PAEL: T223_13170
PFL: PFL_5957(phlD)
PPRC: PFLCHA0_c59110(bcsA)
PPRO: PPC_5904(phlD)
PFE: PSF113_2459(phlD)
PSOS: POS17_5899(phlD)
 » show all
Taxonomy
Reference
1  [PMID:15826166]
  Authors
Achkar J, Xian M, Zhao H, Frost JW.
  Title
Biosynthesis of phloroglucinol.
  Journal
J Am Chem Soc 127:5332-3 (2005)
DOI:10.1021/ja042340g
Reference
2  [PMID:16931521]
  Authors
Zha W, Rubin-Pitel SB, Zhao H.
  Title
Characterization of the substrate specificity of PhlD, a type III polyketide synthase from Pseudomonas fluorescens.
  Journal
J Biol Chem 281:32036-47 (2006)
DOI:10.1074/jbc.M606500200
  Sequence
[pfl:PFL_5957]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.253
IUBMB Enzyme Nomenclature: 2.3.1.253
ExPASy - ENZYME nomenclature database: 2.3.1.253
BRENDA, the Enzyme Database: 2.3.1.253

DBGET integrated database retrieval system