KEGG   ENZYME: 2.3.1.253
Entry
EC 2.3.1.253                Enzyme                                 
Name
phloroglucinol synthase;
phlD (gene name)
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
malonyl-CoA:malonyl-CoA malonyltransferase (decarboxylating, phloroglucinol-forming)
Reaction(IUBMB)
3 malonyl-CoA = phloroglucinol + 3 CO2 + 3 CoA [RN:R10967]
Reaction(KEGG)
R10967
Substrate
malonyl-CoA [CPD:C00083]
Product
phloroglucinol [CPD:C02183];
CO2 [CPD:C00011];
CoA [CPD:C00010]
Comment
The enzyme, characterized from the bacterium Pseudomonas protegens Pf-5, is a type III polyketide synthase. The mechanism involves the cyclization of an activated 3,5-dioxoheptanedioate intermediate. The enzyme exhibits broad substrate specificity, and can accept C4-C12 aliphatic acyl-CoAs and phenylacetyl-CoA as the starter molecules, forming 6-(polyoxoalkyl)-alpha-pyrones by sequential condensation with malonyl-CoA.
History
EC 2.3.1.253 created 2016
Orthology
K15431  phloroglucinol synthase
Genes
YMOHRD69_02465
VNIVIBNI_A2441(phlD)
VCYIX92_07225
VCTJV59_31265
PAGPLES_25771
PAFPAM18_2382
PAELT223_13170
PFLPFL_5957(phlD)
PPRCPFLCHA0_c59110(bcsA)
PPROPPC_5904(phlD)
PFEPSF113_2459(phlD)
PBAPSEBR_a2319
PSOSPOS17_5899(phlD)
POJPtoMrB4_28190
PBZGN234_00500
PTWTUM18999_30760
SPSHFM037_15230
PRRAT705_00165
PLZS4054249_08040
PVBJ5X90_12575
CVCBKX93_21155
 » show all
Reference
1  [PMID:15826166]
  Authors
Achkar J, Xian M, Zhao H, Frost JW.
  Title
Biosynthesis of phloroglucinol.
  Journal
J Am Chem Soc 127:5332-3 (2005)
DOI:10.1021/ja042340g
Reference
2  [PMID:16931521]
  Authors
Zha W, Rubin-Pitel SB, Zhao H
  Title
Characterization of the substrate specificity of PhlD, a type III polyketide synthase from Pseudomonas fluorescens.
  Journal
J Biol Chem 281:32036-47 (2006)
DOI:10.1074/jbc.M606500200
  Sequence
[pfl:PFL_5957]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.253
IUBMB Enzyme Nomenclature: 2.3.1.253
ExPASy - ENZYME nomenclature database: 2.3.1.253
BRENDA, the Enzyme Database: 2.3.1.253

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