KEGG   ENZYME: 2.3.1.258Help
Entry
EC 2.3.1.258                Enzyme                                 

Name
N-terminal methionine Nalpha-acetyltransferase NatE;
NAA50 (gene name);
NAT5;
SAN
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
acetyl-CoA:N-terminal-Met-Ala/Ser/Val/Thr/Lys/Leu/Phe/Tyr-[protein] Met-Nalpha-acetyltransferase
Reaction(IUBMB)
(1) acetyl-CoA + an N-terminal-L-methionyl-L-alanyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-alanyl-[protein] + CoA;
(2) acetyl-CoA + an N-terminal-L-methionyl-L-seryl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-seryl-[protein] + CoA;
(3) acetyl-CoA + an N-terminal-L-methionyl-L-valyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-valyl-[protein] + CoA;
(4) acetyl-CoA + an N-terminal-L-methionyl-L-threonyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-threonyl-[protein] + CoA;
(5) acetyl-CoA + an N-terminal-L-methionyl-L-lysyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-lysyl-[protein] + CoA;
(6) acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[protein] + CoA;
(7) acetyl-CoA + an N-terminal-L-methionyl-L-phenylalanyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-phenylalanyl-[protein] + CoA;
(8) acetyl-CoA + an N-terminal-L-methionyl-L-tyrosyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-tyrosyl-[protein] + CoA
Substrate
acetyl-CoA [CPD:C00024];
N-terminal-L-methionyl-L-alanyl-[protein];
N-terminal-L-methionyl-L-seryl-[protein];
N-terminal-L-methionyl-L-valyl-[protein];
N-terminal-L-methionyl-L-threonyl-[protein];
N-terminal-L-methionyl-L-lysyl-[protein];
N-terminal-L-methionyl-L-leucyl-[protein];
N-terminal-L-methionyl-L-phenylalanyl-[protein];
N-terminal-L-methionyl-L-tyrosyl-[protein]
Product
N-terminal-Nalpha-acetyl-L-methionyl-L-alanyl-[protein];
CoA [CPD:C00010];
N-terminal-Nalpha-acetyl-L-methionyl-L-seryl-[protein];
N-terminal-Nalpha-acetyl-L-methionyl-L-valyl-[protein];
N-terminal-Nalpha-acetyl-L-methionyl-L-threonyl-[protein];
N-terminal-Nalpha-acetyl-L-methionyl-L-lysyl-[protein];
N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[protein];
N-terminal-Nalpha-acetyl-L-methionyl-L-phenylalanyl-[protein];
N-terminal-Nalpha-acetyl-L-methionyl-L-tyrosyl-[protein]
Comment
N-terminal-acetylases (NATs) catalyse the covalent attachment of an acetyl moiety from acetyl-CoA to the free alpha-amino group at the N-terminus of a protein. This irreversible modification neutralizes the positive charge at the N-terminus, makes the N-terminal residue larger and more hydrophobic, and prevents its removal by hydrolysis. It may also play a role in membrane targeting and gene silencing. NatE is found in all eukaryotic organisms and plays an important role in chromosome resolution and segregation. It specifically targets N-terminal L-methionine residues attached to Lys, Val, Ala, Tyr, Phe, Leu, Ser, and Thr. There is some substrate overlap with EC 2.3.1.256, N-terminal methionine Nalpha-acetyltransferase NatC. In addition, the acetylation of Met followed by small residues such as Ser, Thr, Ala, or Val suggests a kinetic competition between NatE and EC 3.4.11.18, methionyl aminopeptidase. The enzyme also has the activity of EC 2.3.1.48, histone acetyltransferase, and autoacetylates several of its own lysine residues.
History
EC 2.3.1.258 created 1989 as EC 2.3.1.88, part transferred 2016 to EC 2.3.1.258
Orthology
K20793  N-alpha-acetyltransferase 50
Genes
HSA: 80218(NAA50)
PTR: 470884(NAA50)
PPS: 100981135(NAA50)
GGO: 101125174(NAA50)
PON: 100171579(NAA50)
NLE: 100590608(NAA50)
MCC: 709764(NAA50)
MCF: 102129158(NAA50)
CSAB: 103228297(NAA50)
RRO: 104660615(NAA50)
RBB: 108519503(NAA50)
CJC: 100410759(NAA50)
SBQ: 101042088(NAA50)
MMU: 72117(Naa50)
MCAL: 110311959(Naa50)
MPAH: 110329584(Naa50)
RNO: 102553099 288108(Naa50)
MUN: 110565145(Naa50)
CGE: 100773295(Naa50)
NGI: 103741923(Naa50)
HGL: 101717688(Naa50)
CCAN: 109698028(Naa50)
OCU: 100347916(NAA50)
TUP: 102480359(NAA50)
VVP: 112913439(NAA50)
AML: 100472027(NAA50)
UMR: 103678813(NAA50)
UAH: 113251480(NAA50)
ORO: 101365472(NAA50)
FCA: 101091471(NAA50)
PTG: 102952994(NAA50)
AJU: 106977758(NAA50)
BTA: 517211(NAA50)
BOM: 102276311(NAA50) 102279372
BIU: 109574855(NAA50)
BBUB: 102396968(NAA50)
PHD: 102338666(NAA50)
CHX: 102185606(NAA50)
OAS: 101115755(NAA50) 114111483
SSC: 100513438(NAA50)
CFR: 102524041(NAA50)
CDK: 105093201(NAA50)
BACU: 103015576 103017541(NAA50)
LVE: 103083185(NAA50)
OOR: 101271269(NAA50)
DLE: 111164119(NAA50)
ECB: 100071372(NAA50)
EPZ: 103552856(NAA50)
EAI: 106829495(NAA50)
MYB: 102251733(NAA50)
MYD: 102758496(NAA50)
MNA: 107527449(NAA50)
HAI: 109387973(NAA50)
RSS: 109448981(NAA50)
DRO: 112320549(NAA50)
PALE: 102898138(NAA50)
LAV: 100664835(NAA50)
TMU: 101355597
MDO: 100013194(NAA50)
SHR: 100934075(NAA50)
PCW: 110222275(NAA50)
OAA: 100086792(NAA50)
GGA: 418319(NAA50)
MGP: 100543284(NAA50)
CJO: 107319460(NAA50)
APLA: 101794683(NAA50)
ACYG: 106045725(NAA50)
TGU: 100226501(NAA50)
LSR: 110480788(NAA50)
SCAN: 103827127(NAA50)
GFR: 102038220(NAA50)
FAB: 101820110(NAA50)
PHI: 102103193(NAA50)
PMAJ: 107211809(NAA50)
CCAE: 111938693(NAA50)
CCW: 104687097(NAA50)
ETL: 114062550(NAA50)
FPG: 101920158(NAA50)
FCH: 102056869(NAA50)
CLV: 102097160(NAA50)
EGZ: 104126577(NAA50)
NNI: 104008654(NAA50)
ACUN: 113480574(NAA50)
AAM: 106497185(NAA50)
ASN: 102374943(NAA50)
AMJ: 102558991(NAA50)
PSS: 102452426(NAA50)
CMY: 102934208(NAA50)
CPIC: 101944196(NAA50)
ACS: 100554642(naa50)
PVT: 110076936(NAA50)
PBI: 103058239(NAA50)
PMUR: 107284114(NAA50)
GJA: 107111860(NAA50)
XLA: 108707810 444177(naa50.L)
XTR: 496547(naa50)
NPR: 108800121(NAA50)
DRE: 445229(naa50)
IPU: 108256434(naa50)
PHYP: 113541272(naa50)
AMEX: 103040640(naa50)
EEE: 113576144(naa50)
TRU: 101062251(naa50)
LCO: 104924969(naa50)
NCC: 104955922(naa50)
MZE: 101464000(naa50)
OLA: 101162486(naa50)
XMA: 102221294(naa50)
XCO: 114137053(naa50)
PRET: 103456643(naa50)
NFU: 107382855(naa50)
KMR: 108230296(naa50)
CSEM: 103377239(naa50)
LCF: 108881741(naa50)
SDU: 111220958(naa50)
HCQ: 109514044(naa50)
BPEC: 110160975(naa50)
MALB: 109960075(naa50)
SASA: 106579447(NAT13) 106590472(NAT13)
ELS: 105021969(naa50)
SFM: 108939472(naa50)
PKI: 111860991(naa50)
LCM: 102350538(NAA50)
CMK: 103177566(naa50)
CIN: 100184886
APLC: 110990398
DME: Dmel_CG12352(san)
DER: 6546984
DPE: 6591038
DSI: Dsimw501_GD15393(Dsim_GD15393)
DWI: 6652327
DAZ: 108617056
DNV: 108654767
DHE: 111605405
MDE: 101892024
AAG: 5566935
AME: 724486
BIM: 100748927
BTER: 100646834
SOC: 105198579
MPHA: 105835096
AEC: 105143574
ACEP: 105623003
PBAR: 105428405
HST: 105188917
DQU: 106743072
CFO: 105248954
LHU: 105676435
PGC: 109856832
OBO: 105286290
PCF: 106788650
NVI: 100120548
MDL: 103568257
TCA: 107397753
DPA: 109543101
NVL: 108569774
BMOR: 732937
PMAC: 106708748
PRAP: 110998311
HAW: 110384515
TNL: 113495549
PXY: 105392948
API: 100163396
AGS: 114124274
CLEC: 106673031
ZNE: 110826825
FCD: 110842120
DPTE: 113799805
CEL: CELE_F40F4.7(F40F4.7)
CBR: CBG14164
BMY: Bm1_06410
TSP: Tsp_01637
PCAN: 112567940
CRG: 105335433
MYI: 110462991
OBI: 106871775
SHX: MS3_01596
EGL: EGR_03101
EPA: 110246078
ADF: 107356298
PDAM: 113665705
SPIS: 111343034
HMG: 100200459
AQU: 100634178
ATH: AT5G11340
CRB: 17882717
CPAP: 110811317
CIT: 102630165
TCC: 18588966
GRA: 105791069
EGR: 104437074
VRA: 106764080
VAR: 108337957
CCAJ: 109814320
CAM: 101508621
LJA: Lj1g3v0948650.1(Lj1g3v0948650.1)
ADU: 107496078
AIP: 107609729
FVE: 101296345
PPER: 18789044
PMUM: 103320050
PAVI: 110769099
PXB: 103927576
ZJU: 107409208
CSV: 101220663
CMO: 103488274
MCHA: 111005202
RCU: 8284851
JCU: 105637684
JRE: 109001803
VVI: 100244109
SOT: 102601173
INI: 109184397
SIND: 105168152
LSV: 111883135
CCAV: 112522058
DCR: 108225330
BVG: 104887712
SOE: 110795837
OSA: 4327733
DOSA: Os01t0610400-01(Os01g0610400)
OBR: 102706887
BDI: 100846813
ATS: 109765512(LOC109765512)
SBI: 8084808
ZMA: 100282487(GNAT26)
SITA: 101753129
DCT: 110093744
PEQ: 110029350
AOF: 109819672
ATR: 18437799
PPP: 112290739
MNG: MNEG_2596
APRO: F751_6581
SCE: YOR253W(NAT5)
ERC: Ecym_2375
KMX: KLMA_30455(NAT5)
NCS: NCAS_0B01180(NCAS0B01180) NCAS_0C01230(NCAS0C01230)
NDI: NDAI_0E01060(NDAI0E01060) NDAI_0K01240(NDAI0K01240)
TPF: TPHA_0G01490(TPHA0G01490)
TBL: TBLA_0B03740(TBLA0B03740)
TDL: TDEL_0A06420(TDEL0A06420)
KAF: KAFR_0A03960(KAFR0A03960)
CAL: CAALFM_CR04070WA(NAT5)
NCR: NCU00576
NTE: NEUTE1DRAFT92946(NEUTE1DRAFT_92946)
MGR: MGG_02993
SSCK: SPSK_09217
MAW: MAC_04823
MAJ: MAA_00808
CMT: CCM_03321
MBE: MBM_05189
ANI: AN8684.2
ANG: ANI_1_1042104(An12g08660)
ABE: ARB_02010
TVE: TRV_05841
PTE: PTT_16098
SPO: SPBC13E7.02(cwf24)
CNE: CNI01640
CNB: CNBH1560
ABP: AGABI1DRAFT112462(AGABI1DRAFT_112462)
ABV: AGABI2DRAFT192431(AGABI2DRAFT_192431)
MGL: MGL_4030
DDI: DDB_G0295721(nat13)
DFA: DFA_03507(nat13)
SMIN: v1.2.008129.t1(symbB.v1.2.008129.t1) v1.2.015199.t1(symbB.v1.2.015199.t1)
SPAR: SPRG_05741
TCR: 504867.40
 » show all
Taxonomy
Reference
1  [PMID:17502424]
  Authors
Hou F, Chu CW, Kong X, Yokomori K, Zou H
  Title
The acetyltransferase activity of San stabilizes the mitotic cohesin at the centromeres in a shugoshin-independent manner.
  Journal
J Cell Biol 177:587-97 (2007)
DOI:10.1083/jcb.200701043
Reference
2  [PMID:18801358]
  Authors
Pimenta-Marques A, Tostoes R, Marty T, Barbosa V, Lehmann R, Martinho RG
  Title
Differential requirements of a mitotic acetyltransferase in somatic and germ line cells.
  Journal
Dev Biol 323:197-206 (2008)
DOI:10.1016/j.ydbio.2008.08.021
Reference
3  [PMID:19744929]
  Authors
Evjenth R, Hole K, Karlsen OA, Ziegler M, Arnesen T, Lillehaug JR
  Title
Human Naa50p (Nat5/San) displays both protein N alpha- and N epsilon-acetyltransferase activity.
  Journal
J Biol Chem 284:31122-9 (2009)
DOI:10.1074/jbc.M109.001347
Reference
4  [PMID:25886145]
  Authors
Van Damme P, Hole K, Gevaert K, Arnesen T
  Title
N-terminal acetylome analysis reveals the specificity of Naa50 (Nat5) and suggests a kinetic competition between N-terminal acetyltransferases and methionine aminopeptidases.
  Journal
Proteomics 15:2436-46 (2015)
DOI:10.1002/pmic.201400575
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.258
IUBMB Enzyme Nomenclature: 2.3.1.258
ExPASy - ENZYME nomenclature database: 2.3.1.258
BRENDA, the Enzyme Database: 2.3.1.258

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