KEGG   ENZYME: 2.3.1.258
Entry
EC 2.3.1.258                Enzyme                                 

Name
N-terminal methionine Nalpha-acetyltransferase NatE;
NAA50 (gene name);
NAT5;
SAN
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
acetyl-CoA:N-terminal-Met-Ala/Ser/Val/Thr/Lys/Leu/Phe/Tyr-[protein] Met-Nalpha-acetyltransferase
Reaction(IUBMB)
(1) acetyl-CoA + an N-terminal-L-methionyl-L-alanyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-alanyl-[protein] + CoA;
(2) acetyl-CoA + an N-terminal-L-methionyl-L-seryl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-seryl-[protein] + CoA;
(3) acetyl-CoA + an N-terminal-L-methionyl-L-valyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-valyl-[protein] + CoA;
(4) acetyl-CoA + an N-terminal-L-methionyl-L-threonyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-threonyl-[protein] + CoA;
(5) acetyl-CoA + an N-terminal-L-methionyl-L-lysyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-lysyl-[protein] + CoA;
(6) acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[protein] + CoA;
(7) acetyl-CoA + an N-terminal-L-methionyl-L-phenylalanyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-phenylalanyl-[protein] + CoA;
(8) acetyl-CoA + an N-terminal-L-methionyl-L-tyrosyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-tyrosyl-[protein] + CoA
Substrate
acetyl-CoA [CPD:C00024];
N-terminal-L-methionyl-L-alanyl-[protein];
N-terminal-L-methionyl-L-seryl-[protein];
N-terminal-L-methionyl-L-valyl-[protein];
N-terminal-L-methionyl-L-threonyl-[protein];
N-terminal-L-methionyl-L-lysyl-[protein];
N-terminal-L-methionyl-L-leucyl-[protein];
N-terminal-L-methionyl-L-phenylalanyl-[protein];
N-terminal-L-methionyl-L-tyrosyl-[protein]
Product
N-terminal-Nalpha-acetyl-L-methionyl-L-alanyl-[protein];
CoA [CPD:C00010];
N-terminal-Nalpha-acetyl-L-methionyl-L-seryl-[protein];
N-terminal-Nalpha-acetyl-L-methionyl-L-valyl-[protein];
N-terminal-Nalpha-acetyl-L-methionyl-L-threonyl-[protein];
N-terminal-Nalpha-acetyl-L-methionyl-L-lysyl-[protein];
N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[protein];
N-terminal-Nalpha-acetyl-L-methionyl-L-phenylalanyl-[protein];
N-terminal-Nalpha-acetyl-L-methionyl-L-tyrosyl-[protein]
Comment
N-terminal-acetylases (NATs) catalyse the covalent attachment of an acetyl moiety from acetyl-CoA to the free alpha-amino group at the N-terminus of a protein. This irreversible modification neutralizes the positive charge at the N-terminus, makes the N-terminal residue larger and more hydrophobic, and prevents its removal by hydrolysis. It may also play a role in membrane targeting and gene silencing. NatE is found in all eukaryotic organisms and plays an important role in chromosome resolution and segregation. It specifically targets N-terminal L-methionine residues attached to Lys, Val, Ala, Tyr, Phe, Leu, Ser, and Thr. There is some substrate overlap with EC 2.3.1.256, N-terminal methionine Nalpha-acetyltransferase NatC. In addition, the acetylation of Met followed by small residues such as Ser, Thr, Ala, or Val suggests a kinetic competition between NatE and EC 3.4.11.18, methionyl aminopeptidase. The enzyme also has the activity of EC 2.3.1.48, histone acetyltransferase, and autoacetylates several of its own lysine residues.
History
EC 2.3.1.258 created 1989 as EC 2.3.1.88, part transferred 2016 to EC 2.3.1.258
Orthology
K20793  N-alpha-acetyltransferase 50
Genes
HSA: 80218(NAA50)
PTR: 470884(NAA50)
PPS: 100981135(NAA50)
GGO: 101125174(NAA50)
PON: 100171579(NAA50)
NLE: 100590608(NAA50)
MCC: 709764(NAA50)
MCF: 102129158(NAA50)
CSAB: 103228297(NAA50)
CATY: 105596280(NAA50)
PANU: 101006365(NAA50) 101009854
RRO: 104660615(NAA50)
RBB: 108519503(NAA50)
TFN: 117083503(NAA50)
PTEH: 111543488(NAA50)
CJC: 100410759(NAA50)
SBQ: 101042088(NAA50)
MMUR: 105883927(NAA50)
MMU: 72117(Naa50)
MCAL: 110311959(Naa50)
MPAH: 110329584(Naa50)
RNO: 288108(Naa50)
MCOC: 116085933(Naa50)
MUN: 110565145(Naa50)
CGE: 100773295(Naa50)
PLEU: 114694972(Naa50)
NGI: 103741923(Naa50)
HGL: 101717688(Naa50)
CCAN: 109698028(Naa50)
OCU: 100347916(NAA50)
TUP: 102480359(NAA50)
CFA: 119867329(NAA50)
VVP: 112913439(NAA50)
VLG: 121484691(NAA50)
AML: 100472027(NAA50)
UMR: 103678813(NAA50)
UAH: 113251480(NAA50)
ORO: 101365472(NAA50)
ELK: 111151391
MPUF: 101694526(NAA50)
EJU: 114214787(NAA50)
MLX: 118004533 118007095(NAA50)
FCA: 101091471(NAA50)
PYU: 121036252(NAA50)
PBG: 122491454(NAA50)
PTG: 102952994(NAA50)
PPAD: 109266407(NAA50)
AJU: 106977758(NAA50)
HHV: 120248334(NAA50)
BTA: 517211(NAA50)
BOM: 102276311(NAA50) 102279372
BIU: 109574855(NAA50)
BBUB: 102396968(NAA50)
CHX: 102185606(NAA50)
OAS: 101115755(NAA50) 114111483
ODA: 120851758(NAA50) 120869153
CCAD: 122423613 122445556(NAA50)
SSC: 100513438(NAA50)
CFR: 102524041(NAA50)
CBAI: 105081571(NAA50)
CDK: 105093201(NAA50)
BACU: 103015576 103017541(NAA50)
LVE: 103083185(NAA50)
OOR: 101271269(NAA50)
DLE: 111164119(NAA50)
PCAD: 102980570 102997042(NAA50)
ECB: 100071372(NAA50)
EPZ: 103552856(NAA50)
EAI: 106829495(NAA50)
MYB: 102251733(NAA50)
MYD: 102758496(NAA50)
MMYO: 118676143(NAA50)
MNA: 107527449(NAA50)
HAI: 109387973(NAA50)
DRO: 112320549(NAA50)
SHON: 118994738(NAA50)
AJM: 119043042(NAA50)
MMF: 118617185(NAA50)
PALE: 102898138(NAA50)
PGIG: 120586638(NAA50)
RAY: 107513704(NAA50)
MJV: 108394402(NAA50)
TOD: 119257513(NAA50)
LAV: 100664835(NAA50)
TMU: 101355597
MDO: 100013194(NAA50)
SHR: 100934075(NAA50)
PCW: 110222275(NAA50)
OAA: 100086792(NAA50)
GGA: 418319(NAA50)
PCOC: 116242337(NAA50)
MGP: 100543284(NAA50)
CJO: 107319460(NAA50)
NMEL: 110399065(NAA50)
APLA: 101794683(NAA50)
ACYG: 106045725(NAA50)
TGU: 100226501(NAA50)
LSR: 110480788(NAA50)
SCAN: 103827127(NAA50)
PMOA: 120506355(NAA50)
GFR: 102038220(NAA50)
FAB: 101820110(NAA50)
PHI: 102103193(NAA50)
PMAJ: 107211809(NAA50)
CCAE: 111938693(NAA50)
CCW: 104687097(NAA50)
ETL: 114062550(NAA50)
FPG: 101920158(NAA50)
FCH: 102056869(NAA50)
CLV: 102097160(NAA50)
EGZ: 104126577(NAA50)
NNI: 104008654(NAA50)
ACUN: 113480574(NAA50)
PADL: 103922122(NAA50)
AAM: 106497185(NAA50)
AROW: 112965952(NAA50)
NPD: 112960088(NAA50)
DNE: 112985086(NAA50)
ASN: 102374943(NAA50)
AMJ: 102558991(NAA50)
CPOO: 109319172(NAA50)
GGN: 109304392(NAA50)
PSS: 102452426(NAA50)
CMY: 102934208(NAA50)
CPIC: 101944196(NAA50)
TST: 117886388(NAA50)
CABI: 116820874(NAA50)
ACS: 100554642(naa50)
PVT: 110076936(NAA50)
PBI: 103058239(NAA50)
PMUR: 107284114(NAA50)
TSR: 106545475(NAA50)
PGUT: 117661873(NAA50)
PMUA: 114587491(NAA50)
ZVI: 118075441(NAA50)
GJA: 107111860(NAA50)
XLA: 108707810(naa50.L) 444177(naa50.S)
XTR: 496547(naa50)
NPR: 108800121(NAA50)
DRE: 445229(naa50)
IPU: 108256434(naa50)
PHYP: 113541272(naa50)
AMEX: 103040640(naa50)
EEE: 113576144(naa50)
TRU: 101062251(naa50)
LCO: 104924969(naa50)
NCC: 104955922(naa50)
CGOB: 115025836(naa50)
ELY: 117264364(naa50)
PLEP: 121960383(naa50)
SLUC: 116037920(naa50)
ECRA: 117938135(naa50)
PFLV: 114549092(naa50)
GAT: 120811497(naa50)
MSAM: 119909948(naa50)
CUD: 121526993(naa50)
MZE: 101464000(naa50)
ONL: 100706388(naa50)
OAU: 116314435(naa50)
OLA: 101162486(naa50)
OML: 112151200(naa50)
XMA: 102221294(naa50)
XCO: 114137053(naa50)
XHE: 116711988(naa50)
PRET: 103456643(naa50)
CVG: 107097946(naa50)
CTUL: 119789011(naa50)
NFU: 107382855(naa50)
KMR: 108230296(naa50)
ALIM: 106513413(naa50)
AOCE: 111583146(naa50)
CSEM: 103377239(naa50)
POV: 109629846(naa50)
LCF: 108881741(naa50)
SDU: 111220958(naa50)
SLAL: 111652286 111662955(naa50)
XGL: 120789659(naa50)
HCQ: 109514044(naa50)
BPEC: 110160975(naa50)
MALB: 109960075(naa50)
SASA: 106579447(NAT13) 106590472(NAT13)
OMY: 110489676 110535359(naa50)
SNH: 120028013(naa50) 120050967
ELS: 105021969(naa50)
SFM: 108939472(naa50)
PKI: 111860991(naa50)
AANG: 118235028(naa50)
LOC: 102695251(naa50)
PSPA: 121302798(naa50)
LCM: 102350538(NAA50)
CMK: 103177566(naa50)
RTP: 109914250(naa50)
BFO: 118413389
BBEL: 109484047
CIN: 100184886
SCLV: 120336569
APLC: 110990398
DME: Dmel_CG12352(san)
DER: 6546984
DSE: 6608658
DSI: Dsimw501_GD15393(Dsim_GD15393)
DAN: 6495399
DSR: 110179303
DPE: 6591038
DWI: 6652327
DGR: 6561147
DAZ: 108617056
DNV: 108654767
DHE: 111605405
DVI: 6625474
CCAT: 101451433
MDE: 101892024
LCQ: 111679621
ACOZ: 120948402
AARA: 120908556
AAG: 5566935
CPII: 120417329
AME: 724486
ACER: 107994509
BIM: 100748927
BBIF: 117209619
BTER: 100646834
CCAL: 108622855
OBB: 114874467
MGEN: 117219968
NMEA: 116426176
CGIG: 122402806
SOC: 105198579
MPHA: 105835096
AEC: 105143574
ACEP: 105623003
PBAR: 105428405
VEM: 105562336
HST: 105188917
DQU: 106743072
CFO: 105248954
FEX: 115237776
LHU: 105676435
PGC: 109856832
OBO: 105286290
PCF: 106788650
NVI: 100120548
CSOL: 105364118
MDL: 103568257
CCIN: 107264151
TCA: 107397753
DPA: 109543101
ATD: 109608892
AGB: 108911223
LDC: 111506684
NVL: 108569774
APLN: 108737985
PPYR: 116182861
OTU: 111423693
BMOR: 732937
BMAN: 114251699
MSEX: 115452643
BANY: 112051305
PMAC: 106708748
PPOT: 106104423
PXU: 106121810
PRAP: 110998311
ZCE: 119832937
HAW: 110384515
TNL: 113495549
PXY: 105392948
API: 100163396
AGS: 114124274
RMD: 113549470
BTAB: 109032264
CLEC: 106673031
HHAL: 106678504
NLU: 111053190
ZNE: 110826825
CSEC: 111861957
FCD: 110842120
DMK: 116919096
PVM: 113801071
HAME: 121874103
EAF: 111694732
DSV: 119455929
RSAN: 119393987
RMP: 119172105
VDE: 111250484
VJA: 111259698
DPTE: 113799805
CSCU: 111634882
PTEP: 107439015
SDM: 118196971
CEL: CELE_F40F4.7(F40F4.7)
BMY: BM_BM3964(Bm3964)
TSP: Tsp_01637
PCAN: 112567940
BGT: 106062755
GAE: 121386348
CRG: 105335433
MYI: 110462991
PMAX: 117341303
OBI: 106871775
EGL: EGR_03101
NVE: 5502899
EPA: 110246078
ATEN: 116291812
ADF: 107356298
AMIL: 114974143
PDAM: 113665705
SPIS: 111343034
DGT: 114526514
HMG: 100200459
AQU: 100634178
ATH: AT5G11340
ALY: 9309576
CRB: 17882717
RSZ: 108818377
CPAP: 110811317
CIT: 102630165
PVY: 116115731
TCC: 18588966
GRA: 105791069
EGR: 104437074
VRA: 106764080
VAR: 108337957
VUN: 114193344
CCAJ: 109814320
APRC: 113874101
CAM: 101508621
LJA: Lj1g3v0948650.1(Lj1g3v0948650.1)
ADU: 107496078
AIP: 107609729
FVE: 101296345
RCN: 112174329
PPER: 18789044
PMUM: 103320050
PAVI: 110769099
PDUL: 117613417
PXB: 103927576
ZJU: 107409208
MNT: 21388158
CSV: 101220663
CMO: 103488274
BHJ: 120075007
MCHA: 111005202
RCU: 8284851
JCU: 105637684
JRE: 109001803
QLO: 115972626
VVI: 100244109
VRI: 117911991
SOT: 102601173
INI: 109184397
ITR: 116022926
SIND: 105168152
EGT: 105967052
LSV: 111883135
CCAV: 112522058
DCR: 108225330
BVG: 104887712
SOE: 110795837
MING: 122086555
NCOL: 116246714
OSA: 4327733
DOSA: Os01t0610400-01(Os01g0610400)
OBR: 102706887
BDI: 100846813
ATS: 109765512
SBI: 8084808
ZMA: 100282487(GNAT26)
SITA: 101753129
PHAI: 112891872
PDA: 103721099
DCT: 110093744
PEQ: 110029350
AOF: 109819672
ATR: 18437799
PPP: 112290739
MNG: MNEG_2596
APRO: F751_6581
SCE: YOR253W(NAT5)
ERC: Ecym_2375
KMX: KLMA_30455(NAT5)
NCS: NCAS_0B01180(NCAS0B01180) NCAS_0C01230(NCAS0C01230)
NDI: NDAI_0E01060(NDAI0E01060) NDAI_0K01240(NDAI0K01240)
TPF: TPHA_0G01490(TPHA0G01490)
TBL: TBLA_0B03740(TBLA0B03740)
TDL: TDEL_0A06420(TDEL0A06420)
KAF: KAFR_0A03960(KAFR0A03960)
CAL: CAALFM_CR04070WA(NAT5)
NCR: NCU00576
NTE: NEUTE1DRAFT92946(NEUTE1DRAFT_92946)
MGR: MGG_02993
SSCK: SPSK_09217
MAW: MAC_04823
MAJ: MAA_00808
CMT: CCM_03321
MBE: MBM_05189
ANI: AN8684.2
ANG: ANI_1_1042104(An12g08660)
ABE: ARB_02010
TVE: TRV_05841
PTE: PTT_16098
SPO: SPBC13E7.02(cwf24)
CNE: CNI01640
CNB: CNBH1560
TASA: A1Q1_06151
ABP: AGABI1DRAFT112462(AGABI1DRAFT_112462)
ABV: AGABI2DRAFT192431(AGABI2DRAFT_192431)
MGL: MGL_4030
MRT: MRET_4123
DDI: DDB_G0295721(nat13)
DFA: DFA_03507(nat13)
SMIN: v1.2.008129.t1(symbB.v1.2.008129.t1) v1.2.015199.t1(symbB.v1.2.015199.t1)
SPAR: SPRG_05741
TCR: 504867.40
MSL: Msil_0706
BMQ: BMQ_2376
BMD: BMD_2338
BMEG: BG04_4749
AMT: Amet_2252
 » show all
Reference
1  [PMID:17502424]
  Authors
Hou F, Chu CW, Kong X, Yokomori K, Zou H
  Title
The acetyltransferase activity of San stabilizes the mitotic cohesin at the centromeres in a shugoshin-independent manner.
  Journal
J Cell Biol 177:587-97 (2007)
DOI:10.1083/jcb.200701043
Reference
2  [PMID:18801358]
  Authors
Pimenta-Marques A, Tostoes R, Marty T, Barbosa V, Lehmann R, Martinho RG
  Title
Differential requirements of a mitotic acetyltransferase in somatic and germ line cells.
  Journal
Dev Biol 323:197-206 (2008)
DOI:10.1016/j.ydbio.2008.08.021
Reference
3  [PMID:19744929]
  Authors
Evjenth R, Hole K, Karlsen OA, Ziegler M, Arnesen T, Lillehaug JR
  Title
Human Naa50p (Nat5/San) displays both protein N alpha- and N epsilon-acetyltransferase activity.
  Journal
J Biol Chem 284:31122-9 (2009)
DOI:10.1074/jbc.M109.001347
Reference
4  [PMID:25886145]
  Authors
Van Damme P, Hole K, Gevaert K, Arnesen T
  Title
N-terminal acetylome analysis reveals the specificity of Naa50 (Nat5) and suggests a kinetic competition between N-terminal acetyltransferases and methionine aminopeptidases.
  Journal
Proteomics 15:2436-46 (2015)
DOI:10.1002/pmic.201400575
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.258
IUBMB Enzyme Nomenclature: 2.3.1.258
ExPASy - ENZYME nomenclature database: 2.3.1.258
BRENDA, the Enzyme Database: 2.3.1.258

DBGET integrated database retrieval system