KEGG   ENZYME: 2.3.1.259
Entry
EC 2.3.1.259                Enzyme                                 
Name
N-terminal methionine Nalpha-acetyltransferase NatF;
NAA60 (gene name)
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
acetyl-CoA:N-terminal-Met-Lys/Ser/Val/Leu/Gln/Ile/Tyr/Thr-[transmembrane protein] Met-Nalpha-acetyltransferase
Reaction(IUBMB)
acetyl-CoA + an N-terminal-L-methionyl-[transmembrane protein] = an N-terminal-Nalpha-acetyl-L-methionyl-[transmembrane protein] + CoA
Substrate
acetyl-CoA [CPD:C00024];
N-terminal-L-methionyl-[transmembrane protein]
Product
N-terminal-Nalpha-acetyl-L-methionyl-[transmembrane protein];
CoA [CPD:C00010]
Comment
N-terminal-acetylases (NATs) catalyse the covalent attachment of an acetyl moiety from acetyl-CoA to the free alpha-amino group at the N-terminus of a protein. This irreversible modification neutralizes the positive charge at the N-terminus, makes the N-terminal residue larger and more hydrophobic, and prevents its removal by hydrolysis. NatF is found only in higher eukaryotes, and is absent from yeast. Unlike other Nat systems the enzyme is located in the Golgi apparatus. It faces the cytosolic side of intracellular membranes, and specifically acetylates transmembrane proteins whose N termini face the cytosol. NatF targets N-terminal L-methionine residues attached to Lys, Ser, Val, Leu, Gln, Ile, Tyr and Thr residues.
History
EC 2.3.1.259 created 1989 as EC 2.3.1.88, part transferred 2016 to EC 2.3.1.259
Orthology
K21121  N-alpha-acetyltransferase 60
Genes
HSA79903(NAA60)
PPS100976892(NAA60)
GGO109025256(NAA60)
PON100442711(NAA60)
NLE100594954(NAA60)
MCC703207(NAA60)
MCF101867007(NAA60)
CSAB103227510(NAA60)
CATY105572081(NAA60)
PANU100997803(NAA60)
TGE112614826(NAA60)
RRO104672297(NAA60)
RBB108538286(NAA60)
TFN117097740(NAA60)
PTEH111551686(NAA60)
CJC100388736(NAA60)
SBQ101047996(NAA60)
CSYR103273673(NAA60)
MMUR105859697(NAA60)
LCAT123632309(NAA60)
OGA100941570(NAA60)
MMU74763(Naa60)
MCAL110311719(Naa60)
MPAH110329609(Naa60)
RNO363545(Naa60)
MCOC116084051(Naa60)
MUN110545966(Naa60)
CGE100765069(Naa60)
MAUA101839658 101842187(Naa60)
PLEU114710626(Naa60)
MORG121463709(Naa60)
AAMP119811353(Naa60)
NGI103744342(Naa60)
HGL101705001(Naa60)
CPOC100715078(Naa60)
CCAN109681688(Naa60) 109702239
DORD105981974(Naa60)
DSP122123751(Naa60)
OCU100339358(NAA60)
OPI101516114(NAA60)
TUP102496365(NAA60)
CFA609456(NAA60)
CLUD112640458(NAA60)
VVP112923257(NAA60)
VLG121487532(NAA60)
AML100475085(NAA60)
UMR103668540(NAA60)
UAH113270868(NAA60)
UAR123783247(NAA60)
ELK111156153
LLV125090762 125107853
MPUF101685377(NAA60)
ORO101368928(NAA60)
EJU114219922(NAA60)
ZCA113933247(NAA60)
MLX118021770(NAA60)
FCA101097199(NAA60)
PYU121019611(NAA60)
PBG122471747(NAA60)
PTG102956593(NAA60)
PPAD109278229(NAA60)
AJU106978800(NAA60)
HHV120236478(NAA60)
BTA514154(NAA60)
BOM102284251(NAA60)
BIU109578595(NAA60)
BBUB102400094(NAA60)
CHX102187019(NAA60)
OAS101120793(NAA60)
ODA120874204(NAA60)
CCAD122433043(NAA60)
SSC100739355(NAA60)
CFR102506796(NAA60)
CBAI105064801(NAA60)
CDK105099669(NAA60)
VPC102533909(NAA60)
BACU103018945(NAA60)
LVE103073215(NAA60)
OOR101283656(NAA60)
DLE111185242(NAA60)
PCAD102993356(NAA60)
PSIU116739524(NAA60)
ECB100065966(NAA60)
EPZ103553021(NAA60)
EAI106826055(NAA60)
MYB102239764 102262291(NAA60)
MYD102754456 102754601(NAA60) 107183288
MMYO118655830(NAA60)
MLF102428072(NAA60) 102437560
MNA107535788(NAA60)
PKL118703176(NAA60)
HAI109376774(NAA60)
DRO112298350(NAA60)
SHON118975761(NAA60)
AJM119056243(NAA60)
PDIC114495534(NAA60)
PHAS123807221(NAA60)
MMF118641634(NAA60)
RFQ117019625(NAA60)
PALE102888501(NAA60)
PGIG120620329(NAA60)
PVP105303104(NAA60)
RAY107521670(NAA60)
MJV108386949(NAA60)
TOD119241990(NAA60)
SARA101536917(NAA60)
LAV100664578(NAA60)
TMU101342825
DNM101412032(NAA60)
MDO100016396(NAA60)
GAS123231664(NAA60)
SHR100927065(NAA60)
PCW110214533(NAA60)
OAA100091367(NAA60)
GGA416659(NAA60)
PCOC116230239(NAA60)
MGP100541164(NAA60)
CJO107320974(NAA60)
NMEL110405663(NAA60)
APLA101801604(NAA60)
ACYG106033570(NAA60)
AFUL116495293(NAA60)
TGU100232229(NAA60)
LSR110481459(NAA60)
SCAN103817756(NAA60)
PMOA120513282(NAA60)
OTC121334682(NAA60)
PRUF121350952(NAA60)
GFR102033337(NAA60)
FAB101815151(NAA60)
PHI102105859(NAA60)
PMAJ107211293(NAA60)
CCAE111935891(NAA60)
CCW104684674(NAA60)
CBRC103620453(NAA60)
ETL114057013(NAA60)
ZAB102064376(NAA60)
ACHL103799025(NAA60)
FPG101923095(NAA60)
FCH102055540(NAA60)
CLV102084596(NAA60)
EGZ104121962(NAA60)
NNI104023458(NAA60)
PLET104621209(NAA60)
PCRI104028903(NAA60)
ACUN113485696(NAA60)
TALA104368492(NAA60)
PADL103917535(NAA60)
ACHC115335856(NAA60)
HALD104325259(NAA60)
CCRI104163607(NAA60)
CSTI104562336(NAA60)
EHS104509418(NAA60)
CMAC104473454(NAA60)
MUI104536646(NAA60)
FGA104081744(NAA60)
GSTE104251303(NAA60)
LDI104344881(NAA60)
MNB103775353(NAA60)
OHA104330973(NAA60)
AAM106482333(NAA60)
AROW112965853(NAA60)
NPD112955694(NAA60)
DNE112987728(NAA60)
ASN102380292(NAA60)
AMJ102565984(NAA60)
CPOO109313462(NAA60)
GGN109290772(NAA60)
PSS102444632(NAA60)
CMY102943802(NAA60)
CPIC101951671(NAA60)
TST117883723(NAA60)
CABI116825517(NAA60)
MRV120372942(NAA60)
ACS100559165(naa60)
PVT110089105(NAA60)
SUND121914645(NAA60)
PBI103053152(NAA60)
PMUR107283982(NAA60)
TSR106551963(NAA60)
PGUT117679470(NAA60)
VKO123025006(NAA60)
PMUA114584111(NAA60)
ZVI118093249(NAA60)
GJA107109325(NAA60)
STOW125430626(NAA60)
XLA108701639(naa60.L) 108703194
XTR100127619(naa60)
NPR108791085(NAA60)
RTEM120943346(NAA60)
BBUF121009001(NAA60)
BGAR122944989(NAA60)
DRE570640(nat15)
SRX107733872 107738623
SANH107685925 107690248
SGH107568044 107590614
CCAR109058234 109058290
CAUA113043059 113046988
PPRM120489903(nat15)
MAMB125275827(nat15)
IPU108255159(nat15)
PHYP113546957(naa60)
SMEO124397307(nat15)
TFD113637465(nat15)
AMEX103022043(naa60)
EEE113572845(naa60)
TRU101074253(naa60)
TNGGSTEN00021054G001
LCO104926826(naa60)
NCC104955267
CGOB115012033(naa60) 115028645
ELY117267746(nat15)
EFO125879143(nat15)
PLEP121956809(nat15)
SLUC116063824(nat15)
ECRA117957722(nat15)
ESP116702602(naa60)
PFLV114569535(naa60)
GAT120827433 120827435
PPUG119220215(nat15)
MSAM119882721(nat15)
CUD121503921(nat15)
ALAT119014301(nat15)
MZE101487939(naa60)
ONL100707283(naa60)
OAU116309429(nat15)
OLA101169610(naa60)
OML112138564(nat15)
XMA102217265(naa60)
XCO114148394(naa60)
XHE116736068(naa60)
PRET103468313(naa60)
PFOR103152598(naa60)
PLAI106952981(naa60)
PMEI106933053(naa60)
GAF122821386(nat15)
CVG107103770(naa60)
CTUL119772710(nat15)
GMU124862369 124863732
NFU107376190(naa60)
KMR108249598(nat15)
ALIM106517795(naa60)
NWH119421979(nat15)
AOCE111566375(naa60)
MCEP124998275(nat15)
CSEM103393634(naa60)
POV109642736 109648296
SSEN122785731(nat15)
HHIP117766368(nat15)
HSP118123740(nat15)
LCF108889280 108898610 108898613
SDU111223769(naa60)
SLAL111662518(naa60)
XGL120788213(nat15)
HCQ109530002(naa60)
BPEC110171429(naa60)
MALB109967557(naa60)
BSPL114860692(nat15)
SASA100380402(natu) 106591578 106592154
OTW112246363 121845624(nat15)
OMY110538863(nat15) 118938383
OGO124020919
SALP112078584(naa60)
SNH120041655 120041656
CCLU121551617 121556142
ELS105013475(naa60)
SFM108935797(naa60)
PKI111849109(naa60)
AANG118216832(nat15)
LOC102686281(naa60)
PSPA121301398 121301657
ARUT117417922(nat15) 117973648
LCM102354212(NAA60)
RTP109927099(naa60)
BFO118428811
BBEL109485479
CIN100185125
SCLV120334697
SPU594473
APLC110976163
SKO100371949
DMEDmel_CG18177(Naa60)
DER6543971
DSE6605093
DSIDsimw501_GD14213(Dsim_GD14213)
DYADyak_GE20874
DAN6507304
DSR110190545
DPO6900739
DPE6602778
DMN108153230
DWI6645291
DGR6556871
DMODmoj_GI13029
DAZ108613067
DNV108651276
DHE111598677
DVI6622168
CCAT101449976
BOD106618533
MDE101893918
SCAC106089417
LCQ111679398
LSQ119604910
AGAAgaP_AGAP005192
ACOZ120953120
AARA120895660
ASTE118509345
AALB109424152 109430834
CQUCpipJ_CPIJ006574
CPII120414442
BCOO119079620
AME552443
ACER108004404
ALAB122715497
BIM100749452
BBIF117212491
BVK117242968
BVAN117155112
BTER100642384
BPYO122574947
CCAL108629193
OBB114875540
MGEN117223495
NMEA116429185
CGIG122396262
SOC105207988
MPHA105830440
AEC105154632
ACEP105619513
PBAR105423977
VEM105559145
HST105191015
DQU106741867
CFO105249139
FEX115235549
LHU105677576
PGC109857933
OBO105286510
PCF106784002
PFUC122525198
VPS122627854
NVI100123019
CSOL105368318
TPRE106652345
MDL103568252
CGLO123274173
DAM107036809
AGIF122851431
LHT122510729
CCIN107264045
TCA660507
DPA109540036
SOY115878163
ATD109599772 109607222
CSET123322549
AGB108915693
LDC111506270
NVL108563781
PPYR116173279
OTU111425755
BMOR101739089
BMAN114247277
MSEX115439982
DPLKGM_213607
BANY112043127
PMAC106719159
PPOT106109820
PXU106121417
PRAP111003750
ZCE119840814
HAW110379620
HZE124634873
TNL113501357
SLIU111359071
OFU114361640
PXY105386420
API100167289
DNX107167488
AGS114120025
RMD113560070
BTAB109043101
DCI103512157 113472871
CLEC106663058
HHAL106692194
NLU111048455
PHUPhum_PHUM042560
FOC113206013
TPAL117654310
ZNE110838665
CSEC111875091
FCD110848454
DPXDAPPUDRAFT_304318
DMK116915784
PVM113807425
PJA122264355
PCHN125047504
HAME121864131
PCLA123745406
PTRU123499274
EAF111708584
LSM121121182
PPOI119101347 119101549
ISCIscW_ISCW024321
DSV119452863
RSAN119400509
VDE111245172
VJA111260039
TUT107360104
DPTE113791029
DFR124498034
CSCU111613357
PTEP107441773
SDM118190914
CELCELE_F30F8.10(F30F8.10)
CBRCBG_23731
BMYBM_BM3712(Bm3712)
LOALOAG_08250
NAINECAME_01460 NECAME_10635
TSPTsp_07897 Tsp_10027
HROHELRODRAFT_64313
LGILOTGIDRAFT_210596
PCAN112567066
BGT106057091
GAE121378255
HRF124138613
HRJ124289304
CRG105340306
MYI110466956
PMAX117325234
MMER123564563
OBI106883587
OSN115211601
LAK106158510
EGLEGR_06272
NVE5504662
EPA110242945
ATEN116293015
ADF107341446
AMIL114959461
PDAM113679321
SPIS111321648
DGT114541778 114543482
XEN124448453
HMG105846977
TADTRIADDRAFT_20891
AQU100634882
LBULBUL_1635
LDELDBND_1657
 » show all
Reference
1  [PMID:21750686]
  Authors
Van Damme P, Hole K, Pimenta-Marques A, Helsens K, Vandekerckhove J, Martinho RG, Gevaert K, Arnesen T
  Title
NatF contributes to an evolutionary shift in protein N-terminal acetylation and is important for normal chromosome segregation.
  Journal
PLoS Genet 7:e1002169 (2011)
DOI:10.1371/journal.pgen.1002169
  Sequence
[hsa:79903] [dme:Dmel_CG18177]
Reference
2  [PMID:25732826]
  Authors
Aksnes H, Van Damme P, Goris M, Starheim KK, Marie M, Stove SI, Hoel C, Kalvik TV, Hole K, Glomnes N, Furnes C, Ljostveit S, Ziegler M, Niere M, Gevaert K, Arnesen T
  Title
An organellar nalpha-acetyltransferase, naa60, acetylates cytosolic N termini of  transmembrane proteins and maintains Golgi integrity.
  Journal
Cell Rep 10:1362-74 (2015)
DOI:10.1016/j.celrep.2015.01.053
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.259
IUBMB Enzyme Nomenclature: 2.3.1.259
ExPASy - ENZYME nomenclature database: 2.3.1.259
BRENDA, the Enzyme Database: 2.3.1.259

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