KEGG   ENZYME: 2.3.1.281Help
Entry
EC 2.3.1.281                Enzyme                                 

Name
5-hydroxydodecatetraenal polyketide synthase;
cpkABC (gene names)
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
malonyl-CoA:malonyl-CoA malonyltransferase ((2E,5S,6E,8E,10E)-5-hydroxydodeca-2,6,8,10-tetraenal-forming)
Reaction(IUBMB)
6 malonyl-CoA + 5 NADPH + NADH + 6 H+ = (2E,5S,6E,8E,10E)-5-hydroxydodeca-2,6,8,10-tetraenal + 6 CoA + 5 NADP+ + NAD+ + 6 CO2 + 4 H2O [RN:R12337]
Reaction(KEGG)
Substrate
malonyl-CoA [CPD:C00083];
NADPH [CPD:C00005];
NADH [CPD:C00004];
H+ [CPD:C00080]
Product
(2E,5S,6E,8E,10E)-5-hydroxydodeca-2,6,8,10-tetraenal [CPD:C22087];
CoA [CPD:C00010];
NADP+ [CPD:C00006];
NAD+ [CPD:C00003];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
This polyketide synthase enzyme, characterized from the bacterium Streptomyces coelicolor A3(2), catalyses the first reaction in the biosynthesis of coelimycin P1. The enzyme is made of three proteins which together comprise six modules that contain a total of 28 domains. An NADH-dependent terminal reductase domain at the C-terminus of the enzyme catalyses the reductive release of the product.
History
EC 2.3.1.281 created 2019
Orthology
K23365  5-hydroxydodecatetraenal polyketide synthase CpkA
K23366  5-hydroxydodecatetraenal polyketide synthase CpkB
K23367  5-hydroxydodecatetraenal polyketide synthase CpkC
Genes
SCO: SCO6273(SC2C4.03c) SCO6274(SC2C4.04c) SCO6275(SC1G7.01c)
SLV: SLIV_06770 SLIV_06775 SLIV_06780
Taxonomy
Reference
1  [PMID:17009021]
  Authors
Pawlik K, Kotowska M, Chater KF, Kuczek K, Takano E
  Title
A cryptic type I polyketide synthase (cpk) gene cluster in Streptomyces coelicolor A3(2).
  Journal
Arch Microbiol 187:87-99 (2007)
DOI:10.1007/s00203-006-0176-7
  Sequence
Reference
2  [PMID:28451186]
  Authors
Awodi UR, Ronan JL, Masschelein J, de Los Santos ELC, Challis GL
  Title
Thioester reduction and aldehyde transamination are universal steps in actinobacterial polyketide alkaloid biosynthesis.
  Journal
Chem Sci 8:411-415 (2017)
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.281
IUBMB Enzyme Nomenclature: 2.3.1.281
ExPASy - ENZYME nomenclature database: 2.3.1.281
BRENDA, the Enzyme Database: 2.3.1.281

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