KEGG   ENZYME: 2.3.1.282
Entry
EC 2.3.1.282                Enzyme                                 

Name
phenolphthiocerol/phthiocerol/phthiodiolone dimycocerosyl transferase;
papA5 (gene name)
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
mycocerosyl-[mycocerosic acid synthase]:phenolphthiocerol/phthiocerol/phthiodiolone dimycocerosyl transferase
Reaction(IUBMB)
(1) 2 a mycocerosyl-[mycocerosic acid synthase] + a phthiocerol = a dimycocerosyl phthiocerol + 2 holo-[mycocerosic acid synthase];
(2) 2 a mycocerosyl-[mycocerosic acid synthase] + a phthiodiolone = a dimycocerosyl phthiodiolone + 2 holo-[mycocerosic acid synthase];
(3) 2 a mycocerosyl-[mycocerosic acid synthase] + a phenolphthiocerol = a dimycocerosyl phenolphthiocerol + 2 holo-[mycocerosic acid synthase]
Substrate
a mycocerosyl-[mycocerosic acid synthase];
phthiocerol;
phthiodiolone;
phenolphthiocerol
Product
dimycocerosyl phthiocerol;
holo-[mycocerosic acid synthase];
dimycocerosyl phthiodiolone;
dimycocerosyl phenolphthiocerol
Comment
The enzyme, present in certain pathogenic species of mycobacteria, catalyses the transfer of mycocerosic acids to the two hydroxyl groups at the common lipid core of phthiocerol, phthiodiolone, and phenolphthiocerol, forming dimycocerosate esters. The fatty acid precursors of mycocerosic acids are activated by EC 6.2.1.49, long-chain fatty acid adenylyltransferase FadD28, which loads them onto EC 2.3.1.111, mycocerosate synthase. That enzyme extends the precursors to form mycocerosic acids that remain attached until transferred by EC 2.3.1.282.
History
EC 2.3.1.282 created 2019
Orthology
K23414  phenolphthiocerol/phthiocerol/phthiodiolone dimycocerosyl transferase
Genes
MTU: Rv2939(papA5)
MTV: RVBD_2939
MTC: MT3009
MRA: MRA_2965(papA5)
MTF: TBFG_12953
MTB: TBMG_01033
MTK: TBSG_01041
MTZ: TBXG_001021
MTG: MRGA327_18065
MTE: CCDC5079_2699
MTUR: CFBS_3097(papA5)
MTO: MTCTRI2_2996(papA5)
MTD: UDA_2939(papA5)
MTN: ERDMAN_3221(papA5)
MTUC: J113_20430
MTUE: J114_15695
MTUL: TBHG_02869
MTUT: HKBT1_3085(papA5)
MTUU: HKBT2_3090(papA5)
MTQ: HKBS1_3092(papA5)
MBO: BQ2027_MB2964(papA5)
MBB: BCG_2961(papA5)
MBT: JTY_2956(papA5)
MBM: BCGMEX_2956(papA5)
MBX: BCGT_2777
MAF: MAF_29440(papA5)
MMIC: RN08_3238
MCE: MCAN_29611(papA5)
MCQ: BN44_60416(papA)
MCV: BN43_40647(papA)
MCX: BN42_40937(papA)
MCZ: BN45_51351(papA)
MLE: ML2349
MLB: MLBr02349
MUL: MUL_2011(papA5)
MMC: Mmcs_2828
MKM: Mkms_2872
MJL: Mjls_2857
MMI: MMAR_1768(papA5)
MMAE: MMARE11_16880(papA5)
MLI: MULP_01924(papA5)
MHAD: B586_03535
MSHG: MSG_01623(papA5)
MFJ: MFLOJ_16830(papA5)
MSTO: MSTO_18420(papA5)
MSIM: MSIM_07810(papA5)
MNM: MNVM_38420(papA5)
MCOO: MCOO_18260(papA5)
MBAI: MB901379_01585(papA5)
MPSE: MPSD_40660(papA5)
MSHO: MSHO_54720(papA5)
MGAU: MGALJ_49450(papA5)
MLJ: MLAC_28750(papA5)
MSHJ: MSHI_07280(papA5)
MKR: MKOR_16460(papA5) MKOR_25080
MVA: Mvan_3119
MGI: Mflv_3386
MVQ: MYVA_2956(papA5)
MDU: MDUV_13070(papA5)
MAUU: NCTC10437_03014(papA5)
MPSC: MPSYJ_36520(papA5)
MPOF: MPOR_33480(papA5)
MABB: MASS_4932
MSTE: MSTE_04219
MSAL: DSM43276_03871(papA5_2) DSM43276_04662(papA5_3)
MJD: JDM601_2209(papA5)
MTER: 4434518_02132(papA5)
MMIN: MMIN_38070(papA5)
MHIB: MHIB_16580(papA5)
NFA: NFA_14610
NFR: ERS450000_02658(papA5)
NAD: NCTC11293_00161(papA5_1) NCTC11293_02450(papA5_2)
RFA: A3L23_04949(papA5)
SALB: XNR_5892
SHY: SHJG_1266
SRW: TUE45_pSRc_0201(papA5)
SLAU: SLA_0410
SALJ: SMD11_0468
SRJ: SRO_0455
AOI: AORI_5400
AORI: SD37_10955
AMI: Amir_3883
KAL: KALB_5458
ACTN: L083_5715
 » show all
Reference
1  [PMID:15070765]
  Authors
Onwueme KC, Ferreras JA, Buglino J, Lima CD, Quadri LE
  Title
Mycobacterial polyketide-associated proteins are acyltransferases: proof of principle with Mycobacterium tuberculosis PapA5.
  Journal
Proc Natl Acad Sci U S A 101:4608-13 (2004)
DOI:10.1073/pnas.0306928101
  Sequence
[mtu:Rv2939]
Reference
2  [PMID:15123643]
  Authors
Buglino J, Onwueme KC, Ferreras JA, Quadri LE, Lima CD
  Title
Crystal structure of PapA5, a phthiocerol dimycocerosyl transferase from Mycobacterium tuberculosis.
  Journal
J Biol Chem 279:30634-42 (2004)
DOI:10.1074/jbc.M404011200
  Sequence
[mtu:Rv2939]
Reference
3  [PMID:22361940]
  Authors
Chavadi SS, Onwueme KC, Edupuganti UR, Jerome J, Chatterjee D, Soll CE, Quadri LE
  Title
The mycobacterial acyltransferase PapA5 is required for biosynthesis of cell wall-associated phenolic glycolipids.
  Journal
Microbiology 158:1379-87 (2012)
DOI:10.1099/mic.0.057869-0
Reference
4  [PMID:26271001]
  Authors
Touchette MH, Bommineni GR, Delle Bovi RJ, Gadbery JE, Nicora CD, Shukla AK, Kyle JE, Metz TO, Martin DW, Sampson NS, Miller WT, Tonge PJ, Seeliger JC
  Title
Diacyltransferase Activity and Chain Length Specificity of Mycobacterium tuberculosis PapA5 in the Synthesis of Alkyl beta-Diol Lipids.
  Journal
Biochemistry 54:5457-68 (2015)
DOI:10.1021/acs.biochem.5b00455
  Sequence
[mtu:Rv2939]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.282
IUBMB Enzyme Nomenclature: 2.3.1.282
ExPASy - ENZYME nomenclature database: 2.3.1.282
BRENDA, the Enzyme Database: 2.3.1.282

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