KEGG   ENZYME: 2.3.1.293
Entry
EC 2.3.1.293                Enzyme                                 
Name
meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase I;
kasA (gene name);
beta-ketoacyl-acyl carrier protein synthase KasA
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
ultra-long-chain mono-unsaturated fattyl acyl-[acyl-carrier protein]:malonyl-[acyl-carrier protein] C-acyltransferase (decarboxylating)
Reaction(IUBMB)
an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + a holo-[acyl-carrier protein]
Substrate
ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein];
malonyl-[acyl-carrier protein] [CPD:C01209]
Product
ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein];
CO2 [CPD:C00011];
holo-[acyl-carrier protein] [CPD:C00229]
Comment
The enzyme is part of the fatty acid synthase (FAS) II system of mycobacteria, which extends modified products of the FAS I system, eventually forming meromycolic acids that are incorporated into mycolic acids. Meromycolic acids consist of a long chain, typically 50-60 carbons, which is functionalized by different groups.Two 3-oxoacyl-(acyl carrier protein) synthases function within the FAS II system, encoded by the kasA and kasB genes. The two enzymes share some sequence identity but function independently on separate sets of substrates. KasA differs from KasB [EC 2.3.1.294, meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase II], by preferring shorter (C-22 to C-36) and more saturated (only one double bond) substrates.
History
EC 2.3.1.293 created 2019
Orthology
K11609  beta-ketoacyl ACP synthase
Genes
MTU: Rv2245(kasA) Rv2246(kasB)
MTV: RVBD_2245 RVBD_2246
MTC: MT2305(fabF-1) MT2306(fabF-2)
MRA: MRA_2265(kasA) MRA_2266(kasB)
MTF: TBFG_12274 TBFG_12275
MTB: TBMG_01734 TBMG_01735
MTK: TBSG_01744 TBSG_01745
MTZ: TBXG_001716 TBXG_001717
MTG: MRGA327_13860
MTI: MRGA423_13980 MRGA423_13985
MTE: CCDC5079_2082 CCDC5079_2083
MTUR: CFBS_2378 CFBS_2379(kasB)
MTL: CCDC5180_2054 CCDC5180_2055
MTO: MTCTRI2_2281(kasA) MTCTRI2_2282(kasB)
MTD: UDA_2245(kasA) UDA_2246(kasB)
MTN: ERDMAN_2470(kasA) ERDMAN_2471(kasB)
MTJ: J112_12055 J112_12060
MTUB: MT7199_2277 MT7199_2278
MTUE: J114_12035 J114_12040
MTX: M943_11630 M943_11635
MTUH: I917_15810
MTUL: TBHG_02197 TBHG_02198
MTUT: HKBT1_2369 HKBT1_2370(kasB)
MTUU: HKBT2_2371 HKBT2_2372(kasB)
MTQ: HKBS1_2375 HKBS1_2376(kasB)
MBO: BQ2027_MB2269(kasA) BQ2027_MB2270(kasB)
MBB: BCG_2262(kasA) BCG_2263(kasB)
MBT: JTY_2256(kasA) JTY_2257(kasB)
MBM: BCGMEX_2250(kasA) BCGMEX_2251(kasB)
MBK: K60_023300 K60_023310
MBX: BCGT_2068 BCGT_2069
MAF: MAF_22560(kasA) MAF_22570(kasB)
MMIC: RN08_2493 RN08_2494
MCE: MCAN_22671(kasA) MCAN_22681(kasB)
MCQ: BN44_50188(kasA) BN44_50189(kasB)
MCV: BN43_31488(kasA) BN43_31489(kasB)
MCX: BN42_40169(kasA) BN42_40170(kasB)
MCZ: BN45_50577(kasA) BN45_50578(kasB)
MLE: ML1655(kasA) ML1656(kasB)
MLB: MLBr01655(kasA) MLBr01656(kasB)
MPA: MAP_1998(kasA) MAP_1999(kasB) MAP_3485(kasB)
MAO: MAP4_0292 MAP4_1826 MAP4_1827
MAVI: RC58_09125 RC58_09130
MAVU: RE97_09130 RE97_09135
MAV: MAV_2191 MAV_2192 MAV_4343
MIT: OCO_21780 OCO_21790 OCO_42090
MIA: OCU_21960 OCU_21970 OCU_42010
MID: MIP_03076 MIP_03078 MIP_06344
MYO: OEM_19670 OEM_19680 OEM_42440
MCHI: AN480_11270 AN480_11275 AN480_22070
MIR: OCQ_20880 OCQ_20900 OCQ_43360
MMAL: CKJ54_09720 CKJ54_09725 CKJ54_20115
MLP: MLM_1920 MLM_1921 MLM_3503
MMAN: MMAN_18020(kasA) MMAN_18030(kasB_1) MMAN_30190(kasB_2)
MSA: Mycsm_00734 Mycsm_03980 Mycsm_03981
MUL: MUL_1303(kasB) MUL_1304(kasA)
MMI: MMAR_3338(kasA) MMAR_3339(kasB)
MMAE: MMARE11_32390(kasA) MMARE11_32400(kasB)
MLI: MULP_03599(kasA) MULP_03600(kasB)
MPSE: MPSD_34150(kasA) MPSD_34160(kasB)
MSHO: MSHO_47500(kasA) MSHO_47510(kasB)
MMC: Mmcs_0783 Mmcs_3366 Mmcs_3367
MKM: Mkms_0798 Mkms_3428 Mkms_3429
MJL: Mjls_0779 Mjls_3377 Mjls_3378
MMM: W7S_10240 W7S_10245 W7S_21055
MKN: MKAN_03345 MKAN_03350
MYV: G155_18575 G155_18580 G155_24920
MYE: AB431_04975 AB431_19260 AB431_19265
MHAD: B586_09890 B586_09895
MDX: BTO20_06565 BTO20_13085 BTO20_13090
MSHG: MSG_01053(kasB_1) MSG_03170(kasA) MSG_03171(kasB_2)
MFJ: MFLOJ_00450(kasB_1) MFLOJ_00460(kasA) MFLOJ_23320(kasB_2)
MSTO: MSTO_01110(kasB_1) MSTO_01120(kasA) MSTO_27280(kasB_2)
MSIM: MSIM_14500(kasB_1) MSIM_45150(kasB_2) MSIM_45160(kasA)
MSAK: MSAS_12200(kasA) MSAS_12210(kasB_1) MSAS_12510(kasB_2)
MKU: I2456_16055 I2456_16060 I2456_16415
MLW: MJO58_05580(kasB) MJO58_16830(kasA) MJO58_16835(kasB)
MGRO: FZ046_00350 FZ046_10765 FZ046_22645 FZ046_22650
MXE: MYXE_20720(kasB) MYXE_20730(kasA) MYXE_43070
MNV: MNVI_22730 MNVI_45150(kasA) MNVI_45160(kasB)
MPAG: C0J29_07930 C0J29_18640 C0J29_18645
MNM: MNVM_02370(kasA) MNVM_02380(kasB) MNVM_40280
MGOR: H0P51_17360 H0P51_17365 H0P51_25650
MCOO: MCOO_12820 MCOO_12830(kasB_1) MCOO_39080(kasB_2)
MBAI: MB901379_03010(kasA_1) MB901379_03011(kasB)
MSEO: MSEO_14160(kasB_1) MSEO_19570(kasA) MSEO_19580(kasB_2)
MHEK: JMUB5695_02081(kasB) JMUB5695_02082(kasA) JMUB5695_04192
MGAU: MGALJ_21310 MGALJ_21320 MGALJ_52620
MLJ: MLAC_22590(kasA) MLAC_22600(kasB)
MBRD: MBRA_10910 MBRA_35610(kasB) MBRA_35620(kasA)
MSHJ: MSHI_10160(kasB) MSHI_10170(kasA)
MMAM: K3U93_14875 K3U93_14880
MSPG: F6B93_13965 F6B93_13970
MOT: LTS72_07815 LTS72_07820
MLM: MLPF_0930(kasA) MLPF_0931(kasB)
MPAA: MKK62_10055(kasB) MKK62_10060(kasA) MKK62_21435
MMEH: M5I08_06355 M5I08_12040(kasB) M5I08_12045(kasA)
MSM: MSMEG_1204 MSMEG_4327 MSMEG_4328
MSG: MSMEI_1170(kasB) MSMEI_4227(kasA) MSMEI_4228(kasB)
MSB: LJ00_05995 LJ00_21445 LJ00_21450
MSN: LI99_05995 LI99_21450 LI99_21455
MSH: LI98_05995 LI98_21455 LI98_21460
MVA: Mvan_3756 Mvan_3757 Mvan_4950
MGI: Mflv_1799 Mflv_2776 Mflv_2777
MSP: Mspyr1_11830 Mspyr1_22160 Mspyr1_22170
MCB: Mycch_3278 Mycch_3279 Mycch_5138
MNE: D174_18305 D174_18310
MYN: MyAD_17950 MyAD_17955
MFT: XA26_37930 XA26_37940 XA26_50520
MPHL: MPHLCCUG_01036(kasB_1) MPHLCCUG_03392(kasA) MPHLCCUG_03393(kasB_2)
MVQ: MYVA_3578 MYVA_3579 MYVA_4769 MYVA_5709
MLL: B1R94_04715 B1R94_10585 B1R94_10590
MRH: MycrhN_0961 MycrhN_4434 MycrhN_4435
MTHN: 4412656_01785(kasB) 4412656_01786(kasA_1)
MHAS: MHAS_00020(kasB_1) MHAS_01448(kasB_2) MHAS_01449(kasA)
MDU: MDUV_08130 MDUV_08140 MDUV_44660
MCHT: MCHIJ_33630 MCHIJ_33640
MDR: MDOR_04350 MDOR_24810 MDOR_24820
MAUU: NCTC10437_03513(kasA) NCTC10437_03514(kasB_1) NCTC10437_04668(kasB_2)
MMAG: MMAD_09900(kasB_1) MMAD_35130 MMAD_35140 MMAD_44180(kasB_2) MMAD_50950
MMOR: MMOR_29430 MMOR_29440 MMOR_56170
MFX: MFAL_13630 MFAL_13640
MAIC: MAIC_34210 MAIC_34220 MAIC_46930
MIJ: MINS_32340 MINS_32350
MALV: MALV_02270 MALV_02280
MTY: MTOK_56270 MTOK_56280
MPSC: MPSYJ_10760 MPSYJ_30180 MPSYJ_30190
MARZ: MARA_52190 MARA_61340 MARA_61350
MGAD: MGAD_02940 MGAD_02950 MGAD_51510
MHEV: MHEL_60250 MHEL_60260
MSAR: MSAR_35160 MSAR_35170
MANY: MANY_19070 MANY_19080 MANY_48670
MAUB: MAUB_50710 MAUB_50720
MPOF: MPOR_39290 MPOR_39300 MPOR_50690(kasB)
MPHU: MPHO_18690 MPHO_18700
MMUC: C1S78_009090 C1S78_009095
MMAT: MMAGJ_40560 MMAGJ_73560 MMAGJ_73570
MBOK: MBOE_35990 MBOE_36000
MFG: K6L26_13125 K6L26_13130 K6L26_26205
MSEI: MSEDJ_47240 MSEDJ_47250 MSEDJ_57810
MFLV: NCTC10271_01898(kasB_2) NCTC10271_01899(kasA) NCTC10271_04318(kasB_3)
MCEE: MCEL_03180 MCEL_03190 MCEL_25410
MBRM: L2Z93_001322 L2Z93_001323(kasA)
MMON: EWR22_04110 EWR22_17420 EWR22_17425
MGO: AFA91_18465 AFA91_18470 AFA91_32665
MHOL: K3U96_10645 K3U96_10650 K3U96_22490
MSEN: K3U95_05495 K3U95_15995 K3U95_16000
MPAE: K0O64_04540 K0O64_18625 K0O64_18630
MRF: MJO55_16900(kasA) MJO55_16905(kasB) MJO55_26740
MDF: K0O62_18260 K0O62_18265
MCRO: MI149_05220 MI149_19450(kasA) MI149_19455(kasB)
MKR: MKOR_04700(kasB) MKOR_04710(kasA)
MPAK: MIU77_06595(kasB) MIU77_06600(kasA)
MAB: MAB_1877c MAB_4608
MMV: MYCMA_0969 MYCMA_2544
MABB: MASS_1864 MASS_4635
MABL: MMASJCM_0440 MMASJCM_1795 MMASJCM_4664
MCHE: BB28_09685 BB28_23520
MIZ: BAB75_10110 BAB75_25995
MSTE: MSTE_01795 MSTE_04780
MSAO: MYCSP_08125 MYCSP_21495
MSAL: DSM43276_01633(kasA_1) DSM43276_04441(kasA_2)
MJD: JDM601_1649(kasB) JDM601_1650(kasA) JDM601_2027(kasB)
MTER: 4434518_01562(kasB_1) 4434518_01563(kasA) 4434518_01976(kasB_2)
MMIN: MMIN_32490(kasB) MMIN_32500(kasA)
MHIB: MHIB_10830(kasB) MHIB_10840(kasA) MHIB_26020
MHER: K3U94_09075 K3U94_09080 K3U94_17975
MVM: MJO54_09165(kasB) MJO54_09170(kasA) MJO54_18130(kasB)
ASD: AS9A_1223(fabF)
NFA: NFA_16170(kasA)
NFR: ERS450000_02543(kasA_1)
NCY: NOCYR_1672(fabF) NOCYR_5164(kasA)
NBR: O3I_001035 O3I_001530 O3I_010905 O3I_039885
NNO: NONO_c06540(kasA1) NONO_c13390(kasA2) NONO_c20820(kasA3) NONO_c39590(kasA4) NONO_c40520(kasA5)
NSL: BOX37_05410 BOX37_07850 BOX37_30445
NSR: NS506_02999 NS506_04587 NS506_06033
NTP: CRH09_07575 CRH09_11630 CRH09_18150
NOZ: DMB37_18610 DMB37_22095 DMB37_22610 DMB37_31465
NOD: FOH10_06500 FOH10_13310 FOH10_19595 FOH10_24550
NAH: F5544_08130 F5544_14420 F5544_42295
NAD: NCTC11293_00291(kasA_1) NCTC11293_02031(kasA_2) NCTC11293_02578(kasA_4)
NWL: NWFMUON74_33840 NWFMUON74_34890 NWFMUON74_51900 NWFMUON74_58450
NIE: KV110_01045 KV110_10935 KV110_36485 KV110_38945
NTC: KHQ06_15840 KHQ06_23050 KHQ06_31165 KHQ06_37615
NHU: H0264_15065 H0264_21500 H0264_22775 H0264_30405 H0264_36750
NYA: LTV02_03220 LTV02_10585 LTV02_18070 LTV02_36510
NGP: LTT66_00620 LTT66_03330 LTT66_09725 LTT66_13895
RHA: RHA1_ro01201(fabF)
RER: RER_36740(fabF)
REY: O5Y_16895
REB: XU06_17105
RQI: C1M55_18105
ROP: ROP_09240(fabF) ROP_12330(fabF)
ROA: Pd630_LPD05302 Pd630_LPD05661
RPY: Y013_06230
RBY: CEJ39_17015
RHB: NY08_584
RAV: AAT18_15865
RFA: A3L23_03833(kasA)
RHS: A3Q41_04409(kasA)
RHW: BFN03_10195
RRZ: CS378_05515
RHU: A3Q40_01110(kasA)
RHQ: IM25_20245
RHOD: AOT96_28425 AOT96_30850
RRT: 4535765_03064(fabF_2)
RCR: NCTC10994_04197(fabF_3)
RTM: G4H71_01320
RKO: JWS14_06230
RGO: KYT97_25490
ROZ: CBI38_11880
RPSK: JWS13_38335 JWS13_40200
RGOR: NMQ04_13090
REQ: REQ_28970(kasA)
GBR: Gbro_3123
GPO: GPOL_c28870(kasA)
GOR: KTR9_3116
GOQ: ACH46_13020
GTA: BCM27_16010
GIT: C6V83_13400
GRU: GCWB2_16225(kasA)
GOM: D7316_00150(kasA)
GAV: C5O27_11985
GOD: GKZ92_14975
GAM: GII34_14920
GPD: GII33_14045
GJI: H1R19_15115
GOI: LK459_18890
GHN: MVF96_15495
GAMI: IHQ52_18855
GMG: NWF22_00635
SPIN: KV203_06400
TPR: Tpau_2468 Tpau_2726
TSM: ASU32_13575 ASU32_15195
TPUL: TPB0596_17510 TPB0596_21010
SRT: Srot_0300 Srot_2738
TOY: FO059_12230
 » show all
Reference
1  [PMID:11600501]
  Authors
Schaeffer ML, Agnihotri G, Volker C, Kallender H, Brennan PJ, Lonsdale JT
  Title
Purification and biochemical characterization of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthases KasA and KasB.
  Journal
J Biol Chem 276:47029-37 (2001)
DOI:10.1074/jbc.M108903200
  Sequence
[mtu:Rv2245]
Reference
2  [PMID:16267284]
  Authors
Bhatt A, Kremer L, Dai AZ, Sacchettini JC, Jacobs WR Jr
  Title
Conditional depletion of KasA, a key enzyme of mycolic acid biosynthesis, leads to mycobacterial cell lysis.
  Journal
J Bacteriol 187:7596-606 (2005)
DOI:10.1128/JB.187.22.7596-7606.2005
Reference
3  [PMID:19604480]
  Authors
Luckner SR, Machutta CA, Tonge PJ, Kisker C
  Title
Crystal structures of Mycobacterium tuberculosis KasA show mode of action within cell wall biosynthesis and its inhibition by thiolactomycin.
  Journal
Structure 17:1004-13 (2009)
DOI:10.1016/j.str.2009.04.012
  Sequence
[mtu:Rv2245]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.293
IUBMB Enzyme Nomenclature: 2.3.1.293
ExPASy - ENZYME nomenclature database: 2.3.1.293
BRENDA, the Enzyme Database: 2.3.1.293

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Gene (553)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (542)   
   KEGG MGENES (10)   
Protein sequence (10)   
   UniProt (5)   
   SWISS-PROT (5)   
Protein domain (5)   
   InterPro (5)   
All databases (568)   

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