KEGG   ENZYME: 2.3.1.293
Entry
EC 2.3.1.293                Enzyme                                 

Name
meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase I;
kasA (gene name);
beta-ketoacyl-acyl carrier protein synthase KasA
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
ultra-long-chain mono-unsaturated fattyl acyl-[acyl-carrier protein]:malonyl-[acyl-carrier protein] C-acyltransferase (decarboxylating)
Reaction(IUBMB)
an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + a holo-[acyl-carrier protein]
Substrate
ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein];
malonyl-[acyl-carrier protein] [CPD:C01209]
Product
ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein];
CO2 [CPD:C00011];
holo-[acyl-carrier protein] [CPD:C00229]
Comment
The enzyme is part of the fatty acid synthase (FAS) II system of mycobacteria, which extends modified products of the FAS I system, eventually forming meromycolic acids that are incorporated into mycolic acids. Meromycolic acids consist of a long chain, typically 50-60 carbons, which is functionalized by different groups.Two 3-oxoacyl-(acyl carrier protein) synthases function within the FAS II system, encoded by the kasA and kasB genes. The two enzymes share some sequence identity but function independently on separate sets of substrates. KasA differs from KasB [EC 2.3.1.294, meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase II], by preferring shorter (C-22 to C-36) and more saturated (only one double bond) substrates.
History
EC 2.3.1.293 created 2019
Orthology
K11609  beta-ketoacyl ACP synthase
Genes
MTU: Rv2245(kasA) Rv2246(kasB)
MTV: RVBD_2245 RVBD_2246
MTC: MT2305(fabF-1) MT2306(fabF-2)
MRA: MRA_2265(kasA) MRA_2266(kasB)
MTF: TBFG_12274 TBFG_12275
MTB: TBMG_01734 TBMG_01735
MTK: TBSG_01744 TBSG_01745
MTZ: TBXG_001716 TBXG_001717
MTG: MRGA327_13860
MTI: MRGA423_13980 MRGA423_13985
MTUR: CFBS_2378 CFBS_2379(kasB)
MTO: MTCTRI2_2281(kasA) MTCTRI2_2282(kasB)
MTD: UDA_2245(kasA) UDA_2246(kasB)
MTN: ERDMAN_2470(kasA) ERDMAN_2471(kasB)
MTUH: I917_15810
MTUT: HKBT1_2369 HKBT1_2370(kasB)
MTUU: HKBT2_2371 HKBT2_2372(kasB)
MBO: BQ2027_MB2269(kasA) BQ2027_MB2270(kasB)
MBB: BCG_2262(kasA) BCG_2263(kasB)
MBT: JTY_2256(kasA) JTY_2257(kasB)
MBM: BCGMEX_2250(kasA) BCGMEX_2251(kasB)
MAF: MAF_22560(kasA) MAF_22570(kasB)
MCE: MCAN_22671(kasA) MCAN_22681(kasB)
MCQ: BN44_50188(kasA) BN44_50189(kasB)
MCV: BN43_31488(kasA) BN43_31489(kasB)
MCX: BN42_40169(kasA) BN42_40170(kasB)
MCZ: BN45_50577(kasA) BN45_50578(kasB)
MLE: ML1655(kasA) ML1656(kasB)
MLB: MLBr01655(kasA) MLBr01656(kasB)
MPA: MAP_1998(kasA) MAP_1999(kasB) MAP_3485(kasB)
MMAN: MMAN_18020(kasA) MMAN_18030(kasB_1) MMAN_30190(kasB_2)
MUL: MUL_1303(kasB) MUL_1304(kasA)
MMI: MMAR_3338(kasA) MMAR_3339(kasB)
MMAE: MMARE11_32390(kasA) MMARE11_32400(kasB)
MLI: MULP_03599(kasA) MULP_03600(kasB)
MSHG: MSG_01053(kasB_1) MSG_03170(kasA) MSG_03171(kasB_2)
MFJ: MFLOJ_00450(kasB_1) MFLOJ_00460(kasA) MFLOJ_23320(kasB_2)
MSTO: MSTO_01110(kasB_1) MSTO_01120(kasA) MSTO_27280(kasB_2)
MSIM: MSIM_14500(kasB_1) MSIM_45150(kasB_2) MSIM_45160(kasA)
MSAK: MSAS_12200(kasA) MSAS_12210(kasB_1) MSAS_12510(kasB_2)
MXE: MYXE_20720(kasB) MYXE_20730(kasA) MYXE_43070
MNV: MNVI_22730 MNVI_45150(kasA) MNVI_45160(kasB)
MNM: MNVM_02370(kasA) MNVM_02380(kasB) MNVM_40280
MCOO: MCOO_12820 MCOO_12830(kasB_1) MCOO_39080(kasB_2)
MBAI: MB901379_03010(kasA_1) MB901379_03011(kasB)
MSEO: MSEO_14160(kasB_1) MSEO_19570(kasA) MSEO_19580(kasB_2)
MPSE: MPSD_34150(kasA) MPSD_34160(kasB)
MSHO: MSHO_47500(kasA) MSHO_47510(kasB)
MLJ: MLAC_22590(kasA) MLAC_22600(kasB)
MBRD: MBRA_10910 MBRA_35610(kasB) MBRA_35620(kasA)
MSHJ: MSHI_10160(kasB) MSHI_10170(kasA)
MKR: MKOR_04700(kasB) MKOR_04710(kasA)
MSG: MSMEI_1170(kasB) MSMEI_4227(kasA) MSMEI_4228(kasB)
MPHL: MPHLCCUG_01036(kasB_1) MPHLCCUG_03392(kasA) MPHLCCUG_03393(kasB_2)
MTHN: 4412656_01785(kasB) 4412656_01786(kasA_1)
MHAS: MHAS_00020(kasB_1) MHAS_01448(kasB_2) MHAS_01449(kasA)
MAUU: NCTC10437_03513(kasA) NCTC10437_03514(kasB_1) NCTC10437_04668(kasB_2)
MSAL: DSM43276_01633(kasA_1) DSM43276_04441(kasA_2)
MJD: JDM601_1649(kasB) JDM601_1650(kasA) JDM601_2027(kasB)
MTER: 4434518_01562(kasB_1) 4434518_01563(kasA) 4434518_01976(kasB_2)
MMIN: MMIN_32490(kasB) MMIN_32500(kasA)
MHIB: MHIB_10830(kasB) MHIB_10840(kasA) MHIB_26020
ASD: AS9A_1223(fabF)
NFA: NFA_16170(kasA)
NFR: ERS450000_02543(kasA_1)
NCY: NOCYR_1672(fabF) NOCYR_5164(kasA)
NNO: NONO_c06540(kasA1) NONO_c13390(kasA2) NONO_c20820(kasA3) NONO_c39590(kasA4) NONO_c40520(kasA5)
NAD: NCTC11293_00291(kasA_1) NCTC11293_02031(kasA_2) NCTC11293_02578(kasA_4)
RHA: RHA1_ro01201(fabF)
RER: RER_36740(fabF)
REY: O5Y_16895
ROP: ROP_09240(fabF) ROP_12330(fabF)
REQ: REQ_28970(kasA)
RHB: NY08_584
RFA: A3L23_03833(kasA)
RHS: A3Q41_04409(kasA)
RHU: A3Q40_01110(kasA)
RRT: 4535765_03064(fabF_2)
RCR: NCTC10994_04197(fabF_3)
GBR: Gbro_3123
GPO: GPOL_c28870(kasA)
GOR: KTR9_3116
GRU: GCWB2_16225(kasA)
GOM: D7316_00150(kasA)
 » show all
Reference
1  [PMID:11600501]
  Authors
Schaeffer ML, Agnihotri G, Volker C, Kallender H, Brennan PJ, Lonsdale JT
  Title
Purification and biochemical characterization of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthases KasA and KasB.
  Journal
J Biol Chem 276:47029-37 (2001)
DOI:10.1074/jbc.M108903200
  Sequence
[mtu:Rv2245]
Reference
2  [PMID:16267284]
  Authors
Bhatt A, Kremer L, Dai AZ, Sacchettini JC, Jacobs WR Jr
  Title
Conditional depletion of KasA, a key enzyme of mycolic acid biosynthesis, leads to mycobacterial cell lysis.
  Journal
J Bacteriol 187:7596-606 (2005)
DOI:10.1128/JB.187.22.7596-7606.2005
Reference
3  [PMID:19604480]
  Authors
Luckner SR, Machutta CA, Tonge PJ, Kisker C
  Title
Crystal structures of Mycobacterium tuberculosis KasA show mode of action within cell wall biosynthesis and its inhibition by thiolactomycin.
  Journal
Structure 17:1004-13 (2009)
DOI:10.1016/j.str.2009.04.012
  Sequence
[mtu:Rv2245]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.293
IUBMB Enzyme Nomenclature: 2.3.1.293
ExPASy - ENZYME nomenclature database: 2.3.1.293
BRENDA, the Enzyme Database: 2.3.1.293

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