KEGG   ENZYME: 2.3.1.295
Entry
EC 2.3.1.295                Enzyme                                 

Name
mycoketide-CoA synthase;
pks12 (gene name)
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
malonyl-CoA/(S)-methylmalonyl-CoA:heptanoyl-CoA malonyltransferase (mycoketide-CoA-forming)
Reaction(IUBMB)
a medium-chain acyl-CoA + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+ = a mycoketide-CoA + 10 CO2 + 10 CoA + 22 NADP+ + 11 H2O
Substrate
medium-chain acyl-CoA [CPD:C21926];
malonyl-CoA [CPD:C00083];
(S)-methylmalonyl-CoA [CPD:C00683];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Product
mycoketide-CoA;
CO2 [CPD:C00011];
CoA [CPD:C00010];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme, found in mycobacteria, is involved in the synthesis of beta-D-mannosyl phosphomycoketides. It is a very large polyketide synthase that contains two complete sets of FAS-like fatty acid synthase modules. It binds an acyl-CoA with 5-9 carbons as a starter unit, and extends it by five rounds of alternative additions of malonyl-CoA and methylmalonyl-CoA extender units. Depending on the starter unit, the enzyme forms mycoketide-CoAs of different lengths.
History
EC 2.3.1.295 created 2019
Orthology
K12436  mycoketide-CoA synthase
Genes
MTU: Rv2048c(pks12)
MTV: RVBD_2048c
MTC: MT2108
MRA: MRA_2063(pks12)
MTF: TBFG_12085
MTB: TBMG_01933
MTK: TBSG_01946
MTZ: TBXG_001919
MTE: CCDC5079_1896
MTUR: CFBS_2169(pks12)
MTO: MTCTRI2_2086(pks12)
MTN: ERDMAN_2261(pks12)
MTUB: MT7199_2079(pks12)
MTUE: J114_10960
MTUL: TBHG_02007
MTUT: HKBT1_2163(pks12)
MTUU: HKBT2_2164(pks12)
MTQ: HKBS1_2168(pks12)
MBO: BQ2027_MB2074C(pks12)
MBB: BCG_2067c(pks12)
MBT: JTY_2062(pks12)
MBM: BCGMEX_2051c(pks12)
MAF: MAF_20630(pks12)
MMIC: RN08_2269
MCE: MCAN_20711(pks12)
MCQ: BN44_40339(pks)
MCV: BN43_31234(pks)
MCX: BN42_30352(pks)
MPA: MAP_1796c(pks12)
MAO: MAP4_2032
MAVI: RC58_10135
MAVU: RE97_10145
MAV: MAV_2450
MIT: OCO_24430
MIA: OCU_24300
MID: MIP_03378
MYO: OEM_22900
MIR: OCQ_22970
MLP: MLM_2156
MMAN: MMAN_15230(pks12)
MUL: MUL_2266(pks12)
MMI: MMAR_3025(pks12)
MMAE: MMARE11_29480(pks12)
MMM: W7S_11795
MLI: MULP_02790(pks12)
MHAD: B586_10880
MSHG: MSG_02930(pks12)
MFJ: MFLOJ_02890(pks12)
MSTO: MSTO_03590(pks12)
MSIM: MSIM_47700(pks12)
MSAK: MSAS_10030(pks12)
MCOO: MCOO_10910(pks12)
MSEO: MSEO_17450(pks12)
MPSE: MPSD_31340(pks12)
MSHO: MSHO_44270(pks12)
MHEK: JMUB5695_02290(fadA_2)
MLJ: MLAC_20360(pks12)
MBRD: MBRA_37540(pks12)
MSHJ: MSHI_12060(pks12)
MMAG: MMAD_53390(pks12)
MARZ: MARA_37220(pks12)
 » show all
Reference
1  [PMID:15611286]
  Authors
Matsunaga I, Bhatt A, Young DC, Cheng TY, Eyles SJ, Besra GS, Briken V, Porcelli SA, Costello CE, Jacobs WR Jr, Moody DB
  Title
Mycobacterium tuberculosis pks12 produces a novel polyketide presented by CD1c to T cells.
  Journal
J Exp Med 200:1559-69 (2004)
DOI:10.1084/jem.20041429
  Sequence
[mtu:Rv2048c]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.295
IUBMB Enzyme Nomenclature: 2.3.1.295
ExPASy - ENZYME nomenclature database: 2.3.1.295
BRENDA, the Enzyme Database: 2.3.1.295

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