KEGG   ENZYME: 2.3.1.301
Entry
EC 2.3.1.301                Enzyme                                 

Name
mycobacterial beta-ketoacyl-[acyl carrier protein] synthase III;
fabH (gene name) (ambiguous);
mycobacterial 3-oxoacyl-[acyl carrier protein] synthase III
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
dodecanoyl-CoA:malonyl-[acyl-carrier protein] C-acyltransferase
Reaction(IUBMB)
dodecanoyl-CoA + a malonyl-[acyl-carrier protein] = a 3-oxotetradecanoyl-[acyl-carrier protein] + CoA + CO2 [RN:R12735]
Reaction(KEGG)
R12735
Substrate
dodecanoyl-CoA [CPD:C01832];
malonyl-[acyl-carrier protein] [CPD:C01209]
Product
3-oxotetradecanoyl-[acyl-carrier protein] [CPD:C05759];
CoA [CPD:C00010];
CO2 [CPD:C00011]
Comment
The enzyme, characterized from mycobacteria, provides a link between the type I and type II fatty acid synthase systems (FAS-I and FAS-II, respectively) found in these organisms. The enzyme acts on medium- and long-chain acyl-CoAs (C12-C16) produced by the FAS-I system, condensing them with malonyl-[acyl-carrier protein] (malonyl-AcpM) and forming starter molecules for the FAS-II system, which elongates them into meromycolic acids. The enzyme has no activity with short-chain acyl-CoAs (e.g. acetyl-CoA), which are used by EC 2.3.1.180, beta-ketoacyl-[acyl-carrier-protein] synthase III, or branched-chain acyl-CoAs, which are used by EC 2.3.1.300, branched-chain beta-ketoacyl-[acyl-carrier-protein] synthase.
History
EC 2.3.1.301 created 2021
Orthology
K11608  mycobacterial beta-ketoacyl-[acyl-carrier-protein] synthase III
Genes
MTU: Rv0533c(fabH)
MTV: RVBD_0533c
MTC: MT0557(fabH)
MRA: MRA_0540(fabH)
MTF: TBFG_10544
MTB: TBMG_00539
MTK: TBSG_00545
MTZ: TBXG_000536
MTG: MRGA327_03370
MTI: MRGA423_03345
MTUR: CFBS_0557(fabH)
MTO: MTCTRI2_0541(fabH)
MTD: UDA_0533c(fabH)
MTUC: J113_03805
MTUE: J114_02855
MTUH: I917_03830
MTUL: TBHG_00531
MTUT: HKBT1_0557(fabH)
MTUU: HKBT2_0557(fabH)
MTQ: HKBS1_0557(fabH)
MBO: BQ2027_MB0547C(fabH)
MBB: BCG_0577c(fabH)
MBT: JTY_0547(fabH)
MBM: BCGMEX_0548c(fabH)
MBX: BCGT_0317
MAF: MAF_05400(fabH)
MMIC: RN08_0596
MCE: MCAN_05361(fabH)
MCQ: BN44_10593(fabH)
MCV: BN43_10584(fabH)
MCX: BN42_20271(fabH)
MCZ: BN45_10598(fabH)
MPA: MAP_4028c(fabH)
MAO: MAP4_4151
MAVI: RC58_20595
MAVU: RE97_20645
MAV: MAV_4612
MIT: OCO_44980
MIA: OCU_44750
MID: MIP_06835
MYO: OEM_45190
MIR: OCQ_46100
MLP: MLM_3777
MMAN: MMAN_33270(fabH_2)
MUL: MUL_0632(fabH)
MMI: MMAR_0879(fabH)
MMAE: MMARE11_08290(fabH)
MMM: W7S_22645
MLI: MULP_00907(fabH)
MSHG: MSG_00757(fabH)
MFJ: MFLOJ_27180(fabH)
MSTO: MSTO_31080(fabH)
MSIM: MSIM_17160(fabH)
MBAI: MB901379_00748(fabH)
MSEO: MSEO_46770(fabH)
MPSE: MPSD_10100(fabH)
MSHO: MSHO_26380(fabH)
MLJ: MLAC_36200(fabH)
MBRD: MBRA_09820(fabH)
MSHJ: MSHI_29050(fabH)
MKR: MKOR_23540(fabH)
MSPG: F6B93_03570(fabH)
MFX: MFAL_31900(fabH)
MIJ: MINS_11360(fabH)
MAUB: MAUB_45000(fabH)
MPHU: MPHO_24330(fabH)
MAB: MAB_1141c
MABB: MASS_1137
MSTE: MSTE_01096(fabH)
MSAL: DSM43276_00997(fabH_1)
MMIN: MMIN_21150(fabH)
MHIB: MHIB_39220(fabH)
ASD: AS9A_2905(fabH) AS9A_2908(fabH3)
NFA: NFA_52820(fabH) NFA_53970(fabH3)
NFR: ERS450000_04471(fabH_4) ERS450000_04599(fabH_5) ERS450000_04925(fabH_6)
NCY: NOCYR_1634(fabH) NOCYR_5108(fabH) NOCYR_5244(fabH)
NNO: NONO_c71510(fabH1) NONO_c72620(fabH2)
NAH: F5544_42075(fabH) F5544_42770(fabH)
NAD: NCTC11293_00255(fabH_1) NCTC11293_00382(fabH_2) NCTC11293_02068(fabH_4)
NWL: NWFMUON74_49000(fabH1) NWFMUON74_67100(fabH_2) NWFMUON74_68100(fabH_3)
RER: RER_11360(fabH)
REY: O5Y_05155
ROP: ROP_52560(fabH)
REQ: REQ_28490(fabB) REQ_40910(fabH)
RHB: NY08_3715
RFA: A3L23_01861(fabH)
RHS: A3Q41_01504(fabH)
RHU: A3Q40_02272(fabH)
RRT: 4535765_04192(fabH_2)
RCR: NCTC10994_00252(fabB_1) NCTC10994_03167(fabH)
GPO: GPOL_c13590(fabH1) GPOL_c13640(fabH2)
GRU: GCWB2_07615(fabH2)
GOM: D7316_01743(fabH_2)
GOD: GKZ92_07235(fabH)
GAM: GII34_06710(fabH) GII34_10700(fabH)
GPD: GII33_06160(fabH) GII33_10350(fabH)
TPR: Tpau_2193
LMOI: VV02_03980
AMYY: YIM_23715(fabH2)
 » show all
Reference
1  [PMID:11278743]
  Authors
Scarsdale JN, Kazanina G, He X, Reynolds KA, Wright HT.
  Title
Crystal structure of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III.
  Journal
J Biol Chem 276:20516-22 (2001)
DOI:10.1074/jbc.M010762200
  Sequence
[mtu:Rv0533c]
Reference
2  [PMID:15713483]
  Authors
Musayev F, Sachdeva S, Scarsdale JN, Reynolds KA, Wright HT.
  Title
Crystal structure of a substrate complex of Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III (FabH) with lauroyl-coenzyme A.
  Journal
J Mol Biol 346:1313-21 (2005)
DOI:10.1016/j.jmb.2004.12.044
  Sequence
[mtu:Rv0533c]
Reference
3  [PMID:16040614]
  Authors
Brown AK, Sridharan S, Kremer L, Lindenberg S, Dover LG, Sacchettini JC, Besra GS.
  Title
Probing the mechanism of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III mtFabH: factors influencing catalysis and substrate specificity.
  Journal
J Biol Chem 280:32539-47 (2005)
DOI:10.1074/jbc.M413216200
  Sequence
[mtu:Rv0533c]
Reference
4  [PMID:18420147]
  Authors
Sachdeva S, Musayev FN, Alhamadsheh MM, Scarsdale JN, Wright HT, Reynolds KA.
  Title
Separate entrance and exit portals for ligand traffic in Mycobacterium tuberculosis FabH.
  Journal
Chem Biol 15:402-12 (2008)
DOI:10.1016/j.chembiol.2008.03.007
  Sequence
[mtu:Rv0533c]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.301
IUBMB Enzyme Nomenclature: 2.3.1.301
ExPASy - ENZYME nomenclature database: 2.3.1.301
BRENDA, the Enzyme Database: 2.3.1.301

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