KEGG   ENZYME: 2.3.1.301
Entry
EC 2.3.1.301                Enzyme                                 
Name
mycobacterial beta-ketoacyl-[acyl carrier protein] synthase III;
fabH (gene name) (ambiguous);
mycobacterial 3-oxoacyl-[acyl carrier protein] synthase III
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
dodecanoyl-CoA:malonyl-[acyl-carrier protein] C-acyltransferase
Reaction(IUBMB)
dodecanoyl-CoA + a malonyl-[acyl-carrier protein] = a 3-oxotetradecanoyl-[acyl-carrier protein] + CoA + CO2 [RN:R12735]
Reaction(KEGG)
R12735;
(other) R13257
Substrate
dodecanoyl-CoA [CPD:C01832];
malonyl-[acyl-carrier protein] [CPD:C01209]
Product
3-oxotetradecanoyl-[acyl-carrier protein] [CPD:C05759];
CoA [CPD:C00010];
CO2 [CPD:C00011]
Comment
The enzyme, characterized from mycobacteria, provides a link between the type I and type II fatty acid synthase systems (FAS-I and FAS-II, respectively) found in these organisms. The enzyme acts on medium- and long-chain acyl-CoAs (C12-C16) produced by the FAS-I system, condensing them with malonyl-[acyl-carrier protein] (malonyl-AcpM) and forming starter molecules for the FAS-II system, which elongates them into meromycolic acids. The enzyme has no activity with short-chain acyl-CoAs (e.g. acetyl-CoA), which are used by EC 2.3.1.180, beta-ketoacyl-[acyl-carrier-protein] synthase III, or branched-chain acyl-CoAs, which are used by EC 2.3.1.300, branched-chain beta-ketoacyl-[acyl-carrier-protein] synthase.
History
EC 2.3.1.301 created 2021
Pathway
ec00074  Mycolic acid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K11608  mycobacterial beta-ketoacyl-[acyl-carrier protein] synthase III
Genes
BQB: J4P68_0030340
MTU: Rv0533c(fabH)
MTV: RVBD_0533c
MTC: MT0557(fabH)
MRA: MRA_0540(fabH)
MTF: TBFG_10544
MTB: TBMG_00539
MTK: TBSG_00545
MTZ: TBXG_000536
MTG: MRGA327_03370
MTI: MRGA423_03345
MTUR: CFBS_0557(fabH)
MTO: MTCTRI2_0541(fabH)
MTD: UDA_0533c(fabH)
MTJ: J112_02855
MTUB: MT7199_0547
MTUC: J113_03805
MTUE: J114_02855
MTX: M943_02765
MTUH: I917_03830
MTUL: TBHG_00531
MTUT: HKBT1_0557(fabH)
MTUU: HKBT2_0557(fabH)
MTQ: HKBS1_0557(fabH)
MBO: BQ2027_MB0547C(fabH)
MBB: BCG_0577c(fabH)
MBT: JTY_0547(fabH)
MBM: BCGMEX_0548c(fabH)
MBK: K60_005660
MBX: BCGT_0317
MAF: MAF_05400(fabH)
MMIC: RN08_0596
MCE: MCAN_05361(fabH)
MCQ: BN44_10593(fabH)
MCV: BN43_10584(fabH)
MCX: BN42_20271(fabH)
MCZ: BN45_10598(fabH)
MORY: MO_000565(fabH)
MPA: MAP_4028c(fabH)
MAO: MAP4_4151
MAVI: RC58_20595
MAVU: RE97_20645
MAV: MAV_4612
MIT: OCO_44980
MIA: OCU_44750
MID: MIP_06835
MYO: OEM_45190
MCHI: AN480_23535
MIR: OCQ_46100
MMAL: CKJ54_21430
MLP: MLM_3777
MMAN: MMAN_33270(fabH_2)
MSER: MTY59_08410(fabH)
MUL: MUL_0632(fabH)
MMI: MMAR_0879(fabH)
MMAE: MMARE11_08290(fabH)
MLI: MULP_00907(fabH)
MPSE: MPSD_10100(fabH)
MSHO: MSHO_26380(fabH)
MMM: W7S_22645
MKN: MKAN_18700
MSHG: MSG_00757(fabH)
MFJ: MFLOJ_27180(fabH)
MSTO: MSTO_31080(fabH)
MSIM: MSIM_17160(fabH)
MKU: I2456_04475
MLW: MJO58_04045
MPAG: C0J29_04745
MGOR: H0P51_04385
MBAI: MB901379_00748(fabH)
MSEO: MSEO_46770(fabH)
MLJ: MLAC_36200(fabH)
MBRD: MBRA_09820(fabH)
MSHJ: MSHI_29050(fabH)
MSPG: F6B93_03570(fabH)
MOT: LTS72_21600
MKY: IWGMT90018_08660(fabH)
MFX: MFAL_31900(fabH)
MIJ: MINS_11360(fabH)
MAUB: MAUB_45000(fabH)
MPHU: MPHO_24330(fabH)
MMUC: C1S78_006165
MKR: MKOR_23540(fabH)
MPAK: MIU77_15455
MAB: MAB_1141c
MMV: MYCMA_0605
MABB: MASS_1137
MABL: MMASJCM_1150
MIZ: BAB75_06060
MSTE: MSTE_01096(fabH)
MSAO: MYCSP_04930
MSAL: DSM43276_00997(fabH_1)
MMIN: MMIN_21150(fabH)
MHIB: MHIB_39220(fabH)
MHER: K3U94_02295
MVM: MJO54_02050
ASD: AS9A_2905(fabH) AS9A_2908(fabH3)
NFA: NFA_49790(fabH2) NFA_52820(fabH) NFA_53970(fabH3)
NFR: ERS450000_04471(fabH_4) ERS450000_04599(fabH_5) ERS450000_04925(fabH_6)
NCY: NOCYR_1634(fabH) NOCYR_5108(fabH) NOCYR_5244(fabH)
NBR: O3I_037595 O3I_039605 O3I_040210
NNO: NONO_c71510(fabH1) NONO_c72620(fabH2)
NSL: BOX37_05545 BOX37_30280 BOX37_30855
NSR: NS506_00291 NS506_01121 NS506_05990
NTP: CRH09_37520 CRH09_38000
NOZ: DMB37_18410 DMB37_19015
NOD: FOH10_19325 FOH10_33185 FOH10_33695
NAH: F5544_42075(fabH) F5544_42770(fabH)
NAD: NCTC11293_00255(fabH_1) NCTC11293_00382(fabH_2) NCTC11293_02068(fabH_4)
NWL: NWFMUON74_49000(fabH1) NWFMUON74_67100(fabH_2) NWFMUON74_68100(fabH_3)
NIE: KV110_36795 KV110_38765 KV110_39345
NTC: KHQ06_03950 KHQ06_04480 KHQ06_30995
NHU: H0264_10545 H0264_37245 H0264_37850
NYA: LTV02_04610 LTV02_33155 LTV02_33715
NGP: LTT66_09560 LTT66_10155
NSPU: IFM12276_59140(fabH_1) IFM12276_60300(fabH_2)
NYU: D7D52_27040 D7D52_29385 D7D52_31335(fabH)
RHA: RHA1_ro05206
RER: RER_11360(fabH)
REY: O5Y_05155
REB: XU06_05400
RQI: C1M55_05605
ROP: ROP_52560(fabH)
ROA: Pd630_LPD01731
RPY: Y013_11460
RBY: CEJ39_22365
RHB: NY08_3715
RAV: AAT18_22395
RHW: BFN03_00840
RRZ: CS378_12215
RHU: A3Q40_02272(fabH)
RHQ: IM25_03165
RHOD: AOT96_16005
RRT: 4535765_04192(fabH_2)
RCR: NCTC10994_00252(fabB_1) NCTC10994_03167(fabH)
RTM: G4H71_06280
RKO: JWS14_28525 JWS14_39915
RGO: KYT97_13280
ROZ: CBI38_25285
RPSK: JWS13_16060
RGOR: NMQ04_18240
RHOP: D8W71_03360
RHOO: RA302_05500
RHOU: KZJ41_23835
RHOW: A4U64_02165
RHOJ: JVH1_10270
RHOS: QWW67_12480
RHOQ: QWW98_12490
RHOY: QNA09_19735
RHOH: CPI83_23590
RHOX: RBB84_16245
RFA: A3L23_01861(fabH)
RHS: A3Q41_01504(fabH)
REQ: REQ_28490(fabB) REQ_40910(fabH)
PDEF: P9209_07030
PSOW: ABI214_17015 ABI214_17510
WHR: OG579_12070 OG579_20335
GBR: Gbro_1530 Gbro_1536
GPO: GPOL_c13590(fabH1) GPOL_c13640(fabH2)
GOR: KTR9_1451 KTR9_1454
GOQ: ACH46_04630 ACH46_04660
GTA: BCM27_08040 BCM27_08055
GOC: CXX93_17340 CXX93_17380
GIT: C6V83_05515 C6V83_05535
GRU: GCWB2_07615(fabH2)
GOM: D7316_01743(fabH_2)
GAV: C5O27_20195
GOD: GKZ92_07235(fabH)
GOS: QAD21_07235 QAD21_07255
GOJ: GR168_07090(fabH) GR168_07105(fabH)
GAM: GII34_06710(fabH) GII34_10700(fabH)
GPD: GII33_06160(fabH) GII33_10350(fabH)
GJI: H1R19_07445 H1R19_07460
GOI: LK459_03245 LK459_03250
GHN: MVF96_07450 MVF96_07470
GAMI: IHQ52_10380
GMG: NWF22_18095 NWF22_18100
GSI: P5P27_03525 P5P27_03565
SPIN: KV203_02315 KV203_02375
TPR: Tpau_2193
TSM: ASU32_11935
TPUL: TPB0596_23890(fabH1)
TSD: MTP03_24850(fabH1)
DTM: BJL86_3010
DIT: C3V38_03975
DIZ: CT688_14605
DPC: A6048_15280
DLU: A6035_14580
DKN: NHB83_14780
DCD: VYL96_14230
TOY: FO059_01830
LMOI: VV02_03980
NSN: EXE58_08650
NOQ: LN652_12270
NRN: SHK17_20345
AMYY: YIM_23715(fabH2)
 » show all
Reference
1  [PMID:11278743]
  Authors
Scarsdale JN, Kazanina G, He X, Reynolds KA, Wright HT.
  Title
Crystal structure of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III.
  Journal
J Biol Chem 276:20516-22 (2001)
DOI:10.1074/jbc.M010762200
  Sequence
[mtu:Rv0533c]
Reference
2  [PMID:15713483]
  Authors
Musayev F, Sachdeva S, Scarsdale JN, Reynolds KA, Wright HT.
  Title
Crystal structure of a substrate complex of Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III (FabH) with lauroyl-coenzyme A.
  Journal
J Mol Biol 346:1313-21 (2005)
DOI:10.1016/j.jmb.2004.12.044
  Sequence
[mtu:Rv0533c]
Reference
3  [PMID:16040614]
  Authors
Brown AK, Sridharan S, Kremer L, Lindenberg S, Dover LG, Sacchettini JC, Besra GS.
  Title
Probing the mechanism of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III mtFabH: factors influencing catalysis and substrate specificity.
  Journal
J Biol Chem 280:32539-47 (2005)
DOI:10.1074/jbc.M413216200
  Sequence
[mtu:Rv0533c]
Reference
4  [PMID:18420147]
  Authors
Sachdeva S, Musayev FN, Alhamadsheh MM, Scarsdale JN, Wright HT, Reynolds KA.
  Title
Separate entrance and exit portals for ligand traffic in Mycobacterium tuberculosis FabH.
  Journal
Chem Biol 15:402-12 (2008)
DOI:10.1016/j.chembiol.2008.03.007
  Sequence
[mtu:Rv0533c]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.301
IUBMB Enzyme Nomenclature: 2.3.1.301
ExPASy - ENZYME nomenclature database: 2.3.1.301
BRENDA, the Enzyme Database: 2.3.1.301

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Pathway (8)   
   KEGG PATHWAY (6)   
   KEGG MODULE (2)   
Chemical substance (7)   
   KEGG COMPOUND (7)   
Chemical reaction (4)   
   KEGG REACTION (2)   
   KEGG RCLASS (2)   
Gene (254)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (253)   
Protein sequence (147)   
   UniProt (137)   
   SWISS-PROT (10)   
Protein domain (4)   
   InterPro (4)   
All databases (424)   

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