KEGG   ENZYME: 2.3.1.82
Entry
EC 2.3.1.82                 Enzyme                                 

Name
aminoglycoside 6'-N-acetyltransferase;
aminoglycoside N6'-acetyltransferase;
aminoglycoside-6'-acetyltransferase;
aminoglycoside-6-N-acetyltransferase;
kanamycin acetyltransferase
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
acetyl-CoA:kanamycin-B N6'-acetyltransferase
Reaction(IUBMB)
acetyl-CoA + kanamycin-B = CoA + N6'-acetylkanamycin-B [RN:R01889]
Reaction(KEGG)
R01889
Substrate
acetyl-CoA [CPD:C00024];
kanamycin B [CPD:C00825]
Product
CoA [CPD:C00010];
N6'-acetylkanamycin-B [CPD:C03154]
Comment
The antibiotics kanamycin A, kanamycin B, neomycin, gentamicin C1a, gentamicin C2 and sisomicin are substrates. The antibiotic tobramycin, but not paromomycin, can also act as acceptor. The 6-amino group of the purpurosamine ring is acetylated.
History
EC 2.3.1.82 created 1976 as EC 2.3.1.55, transferred 1999 to EC 2.3.1.82, modified 1999, modified 2015
Orthology
K00663  aminoglycoside 6'-N-acetyltransferase
K18815  aminoglycoside 6'-N-acetyltransferase I
K18816  aminoglycoside 6'-N-acetyltransferase I
K19278  aminoglycoside 6'-N-acetyltransferase Ib
K19301  aminoglycoside 6'-N-acetyltransferase II
K19883  bifunctional aminoglycoside 6'-N-acetyltransferase / aminoglycoside 2''-phosphotransferase
Genes
LCQ: 111684168
ECOS: EC958_A0125(aac(6')-Ib-cr)
ECOA: APECO78_02670
ECOJ: P423_25755
STY: STY1445(aac)
STT: t1528(aac)
SEX: STBHUCCB_16260
SENT: TY21A_07755
STM: STM1619
SEO: STM14_1958
SEY: SL1344_1549(aac)
SEF: UMN798_1695(aac)
SENR: STMDT2_15421(aac)
SEND: DT104_15901(aac)
SENI: CY43_08265
SPT: SPA1250(aac)
SEK: SSPA1159
SEI: SPC_2116
SEC: SCH_1615(aac6)
SHB: SU5_02231
SENS: Q786_07130
SED: SeD_A1723
SEG: SG1504
SEL: SPUL_1430
SET: SEN1437
SENA: AU38_07425
SENO: AU37_07420
SENV: AU39_07430
SENQ: AU40_08350
SENL: IY59_07610
SEEP: I137_06450
SENE: IA1_08020
SBG: SBG_1446(aac)
SBZ: A464_1655
SALZ: EOS98_11285(aac(6'))
CTU: CTU_18420
KPX: PMK1_ndm00058(aacA4)
EAR: CCG28763
CFAR: CI104_19120(aacA4) CI104_25585(aacA4) CI104_26865(aacA4)
KAS: KATP_49760(aac(6)-IIc)
METY: MRY16398_p20050(aacA4'-3)
YMO: HRD69_15950(aac(6'))
SMAR: SM39_3758
SERF: L085_06890
PAY: PAU_02905(aac(6')-Ic)
XDO: XDD1_0504
PSI: S70_03395
PSX: DR96_3843
PRG: RB151_017600(aacA4)
ETR: ETAE_2984
ETD: ETAF_2712
ETE: ETEE_1214
SML: Smlt3615
PSUW: WQ53_12250
LAQ: GLA29479_741(aac(6')-Iy)
LEZ: GLE_1155
LEM: LEN_3757
PNC: NCGM2_1758(aac(6')-Iae)
PAEB: NCGM1900_1411(aac(6')1b) NCGM1900_3684(aac(6')1b) NCGM1900_3707(aacA7)
PPUH: B479_26989
PFL: PFL_5759(aacA7)
PPRO: PPC_5705(aacA7)
PEN: PSEEN3982
PSEM: TO66_29130
PSOS: POS17_5695(aacA7)
PANR: A7J50_4574
ABY: ABAYE3630
ABX: ABK1_1145
ABD: ABTW07_3883(aacA4)
ABH: M3Q_2664
ABAU: IX87_12765
ADV: DJ533_01155(aac(6')-Ib)
ADS: FPL17_00505(aac(6')-I)
SBJ: CF168_21235(aacA4)
LPN: lpg2398
LPH: LPV_2710
LPM: LP6_2427(aac)
LPF: lpl2321
LPC: LPC_2079
LPA: lpa_03495
LPE: lp12_2391
LLO: LLO_2680
LCJ: NCTC11976_01989(aacA-aphD)
LWA: SAMEA4504053_2788(aacA-aphD_1) SAMEA4504053_3020(aacA-aphD_2)
LADL: NCTC12735_00473(aacA-aphD)
THIP: N838_16640
HCH: HCH_03105
AHA: AHA_2734
AVR: B565_1403
ASIM: FE240_15555(aac(6')-Ib) FE240_15570
CVI: CV_0230
BMA: BMAA1807
BMAL: DM55_3975
BMAE: DM78_4886
BMAQ: DM76_4671
BMAI: DM57_06980
BMAF: DM51_3500
BMAZ: BM44_3935
BMAB: BM45_4710
BPS: BPSS0262
BPSE: BDL_6157
BPSM: BBQ_5947
BPSU: BBN_3648
BPSD: BBX_5326
BPK: BBK_5462
BPSH: DR55_5095
BPSA: BBU_5863
BPSO: X996_4862
BUT: X994_4362
BOK: DM82_4268
BOC: BG90_3755
PPUL: RO07_02130
PSPU: NA29_23130
PAPI: SG18_02410
PUS: CKA81_11680(aacA4)
HYN: F9K07_12800(aacA4)
RGE: RGE_06410
COJ: CORN_0965
CARM: CARM_0923
RBE: RBE_0462
RBO: A1I_05380
OTT: OTT_0533
WED: wNo_09250
WPI: WP0046
MLO: mll5006
MHUA: MCHK_6077
MES: Meso_3491
SME: SM_b21552(aacC4) SMc02576
EAD: OV14_0938
ATU: Atu2294
ARA: Arad_3392
AGR: AGROH133_07941(aac)
AVI: Avi_3210
RET: RHE_CH02150(aacC2)
RLE: RL0050
RLG: Rleg_4310
RHT: NT26_2539(aacA)
BJA: bll0648(bll0648)
BARH: WN72_01000
RPD: RPD_0417
NWI: Nwi_0128
SNO: Snov_3823
MDI: METDI1154
MEX: Mext_1004
MCH: Mchl_0967
META: Y590_04020
PSF: PSE_0359
CCR: CC_3561
CSE: Cseg_4049
PZU: PHZ_c1111
PDE: Pden_2040
DSH: Dshi_0797
SAL: Sala_0666
ABS: AZOBR_p1140049(aacA)
BAN: BA_2964
BAR: GBAA_2964
BAT: BAS2753
BAI: BAA_3017
BANT: A16_29900
BANR: A16R_30360
BANS: BAPAT_2841
BANV: DJ46_1726
BCE: BC2948
BCA: BCE_3002
BCQ: BCQ_2790
BCX: BCA_3033
BCF: bcf_14450
BCER: BCK_20085
BTL: BALH_2648
BTT: HD73_3023
BTHI: BTK_14975
BTM: MC28_2147
BTI: BTG_04600
BTW: BF38_4116
BWW: bwei_2069
BMYO: BG05_3064
BMYC: DJ92_41
BGY: BGLY_2411
BEO: BEH_06965
ANM: GFC28_818
ANL: GFC29_808
LSP: Bsph_2478
PSYO: PB01_14225
BLEN: NCTC4824_03016(aacA-aphD)
SAV: SAVP026(aacA)
SUW: SATW20_20700(aacA-aphD)
SAUR: SABB_05319
SER: SERP1585(aacA)
SHA: SH1611(aacAaphD)
SSCZ: RN70_01460
SARL: SAP2_19430
MLEN: H3V22_01730 H3V22_14150(aph(2'')-Ia)
PMW: B2K_13595
PRI: PRIO_3091
SNT: SPT_1105
SND: MYY_1110
SPNN: T308_05145
SPV: SPH_1149
SSA: SSA_2384
SSI: SSU1282
SUP: YYK_06160
SSUY: YB51_6540
SSUT: TL13_1276
SSUI: T15_1477
SMB: smi_1111 smi_1704(aacA-aphD)
SIE: SCIM_0920
SANG: SAIN_0857
SANC: SANR_0929
SANS: DK43_05955
SCG: SCI_0852
SCON: SCRE_0780
SCOS: SCR2_0780
STRN: SNAG_1049
OOE: OEOE_1820
EFA: EFA0061
EFAU: EFAU085_02347(aac(6')-Ii)
EFM: M7W_2295
EHR: EHR_07235
ECAS: ECBG_00165
EMU: EMQU_0615
EDU: LIU_12385
EAV: EH197_00430(aph(2'')-Ia)
ELAC: I4Q40_10800(aac(6'))
CRN: CAR_c05190(yyaR)
CML: BN424_985
CPF: CPF_0869
CPR: CPR_0856
CBL: CLK_2787
CBK: CLL_A0989
CBN: CbC4_0309
CBT: CLH_0924
CBF: CLI_0647
CBM: CBF_0615
CBE: Cbei_2323
CBZ: Cbs_2323
CBEI: LF65_03213
CBV: U729_1864
AMT: Amet_2244
CCE: Ccel_1539
BPB: bpr_I2731
STH: STH2617
ELM: ELI_3512
PMIC: NW74_00075
ASD: AS9A_3991
RER: RER_19970
REY: O5Y_09595
ROP: ROP_36450
REQ: REQ_34470
TPR: Tpau_3211
SALB: XNR_1660
SGR: SGR_4266
SGB: WQO_10280
SALS: SLNWT_1712
SFI: SFUL_4182
SLX: SLAV_38065(aacA4)
SKY: D0C37_10035(aac(6'))
SKA: CP970_06620(aac(6'))
SNK: CP967_02430(aac(6'))
SBY: H7H31_17560(aac(6'))
KSK: KSE_10740
CFL: Cfla_3579
NCA: Noca_2344
KFL: Kfla_6093
FAL: FRAAL2461
GOB: Gobs_0023
MMAR: MODMU_4487
KRA: Krad_2030
PDX: Psed_4404
AMI: Amir_4586
MIL: ML5_1935
ASE: ACPL_3609
ACTN: L083_6631
AFS: AFR_25830
CWO: Cwoe_1132
AMR: AM1_4351
GVI: glr2416
GLJ: GKIL_1014
ANA: alr7015
AVA: Ava_0811
NAZ: Aazo_4816
CTHE: Chro_1816
HAU: Haur_0857
STI: Sthe_2306
PBF: CFX0092_A1467(aacA)
TAQ: TO73_1986
MRB: Mrub_0158
OTE: Oter_1485
SACI: Sinac_6048
BIP: Bint_1172
GBA: J421_2517
SLI: Slin_2328
FAE: FAES_4733
EAO: BD94_3730
IAL: IALB_3088
MFC: BRM9_2215
 » show all
Reference
1  [PMID:4614862]
  Authors
Le Goffic F, Martel A.
  Title
[Resistance to aminosides induced by an isoenzyme, kanamycin acetyltransferase]
  Journal
Biochimie 56:893-7 (1974)
DOI:10.1016/S0300-9084(74)80512-2
Reference
2  [PMID:4935296]
  Authors
Benveniste R, Davies J.
  Title
Enzymatic acetylation of aminoglycoside antibiotics by Escherichia coli carrying an R factor.
  Journal
Biochemistry 10:1787-96 (1971)
DOI:10.1021/bi00786a009
Reference
3  [PMID:836013]
  Authors
Dowding JE.
  Title
Mechanisms of gentamicin resistance in Staphylococcus aureus.
  Journal
Antimicrob Agents Chemother 11:47-50 (1977)
DOI:10.1128/aac.11.1.47
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.82
IUBMB Enzyme Nomenclature: 2.3.1.82
ExPASy - ENZYME nomenclature database: 2.3.1.82
BRENDA, the Enzyme Database: 2.3.1.82
CAS: 56467-65-3

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