KEGG   ENZYME: 2.3.1.93
Entry
EC 2.3.1.93                 Enzyme                                 

Name
13-hydroxylupanine O-tigloyltransferase;
tigloyl-CoA:13-hydroxylupanine O-tigloyltransferase;
13-hydroxylupanine acyltransferase
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
(E)-2-methylcrotonoyl-CoA:13-hydroxylupanine O-2-methylcrotonoyltransferase
Reaction(IUBMB)
(E)-2-methylcrotonoyl-CoA + 13-hydroxylupanine = CoA + 13-[(E)-2-methylcrotonoyl]oxylupanine [RN:R04205]
Reaction(KEGG)
R04205
Substrate
(E)-2-methylcrotonoyl-CoA [CPD:C03345];
13-hydroxylupanine [CPD:C02621]
Product
CoA [CPD:C00010];
13-[(E)-2-methylcrotonoyl]oxylupanine
Comment
Benzoyl-CoA and, more slowly, pentanoyl-CoA, 3-methylbutanoyl-CoA and butanoyl-CoA can act as acyl donors. Involved in the synthesis of lupanine alkaloids.
History
EC 2.3.1.93 created 1986, modified 2011
Pathway
ec00960  Tropane, piperidine and pyridine alkaloid biosynthesis
Orthology
K13610  13-hydroxylupinine O-tigloyltransferase
Reference
1  [PMID:24272737]
  Authors
Wink M, Hartmann T
  Title
Enzymatic synthesis of quinolizidine alkaloid esters: a tigloyl-CoA: 13-hydroxylupanine O-tigloyltransferase from Lupinus albus L.
  Journal
Planta 156:560-5 (1982)
DOI:10.1007/BF00392781
Reference
2  [PMID:15659437]
  Authors
Okada T, Hirai MY, Suzuki H, Yamazaki M, Saito K
  Title
Molecular characterization of a novel quinolizidine alkaloid O-tigloyltransferase: cDNA cloning, catalytic activity of recombinant protein and expression analysis in Lupinus plants.
  Journal
Plant Cell Physiol 46:233-44 (2005)
DOI:10.1093/pcp/pci021
  Sequence
Reference
3  [PMID:8195240]
  Authors
Suzuki H, Murakoshi I, Saito K
  Title
A novel O-tigloyltransferase for alkaloid biosynthesis in plants. Purification, characterization, and distribution in Lupinus plants.
  Journal
J Biol Chem 269:15853-60 (1994)
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.93
IUBMB Enzyme Nomenclature: 2.3.1.93
ExPASy - ENZYME nomenclature database: 2.3.1.93
BRENDA, the Enzyme Database: 2.3.1.93
CAS: 85341-00-0

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