Entry |
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Name |
cullin-RING-type E3 NEDD8 transferase;
RBX1 (gene name)
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Class |
Transferases;
Acyltransferases;
Aminoacyltransferases
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Sysname |
[E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine:[cullin] [NEDD8-protein] transferase (isopeptide bond-forming; RING-type)
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Reaction(IUBMB) |
[E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-N6-[NEDD8-protein]-yl-L-lysine
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Substrate |
[E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine;
[cullin]-L-lysine
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Product |
[E2 NEDD8-conjugating enzyme]-L-cysteine;
[cullin]-N6-[NEDD8-protein]-yl-L-lysine
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Comment |
Some RING-type E3 ubiquitin transferase (EC 2.3.2.27) are not able to bind a substrate protein directly. Instead, they form a complex with a cullin scaffold protein and a substrate recognition module, which is named CRL for Cullin-RING-Ligase. The cullin protein needs to be activated by the ubiquitin-like protein NEDD8 in a process known as neddylation. The transfer of NEDD8 from a NEDD8-specific E2 enzyme onto the cullin protein is a secondary function of the RING-type E3 ubiquitin transferase in the CRL complex. The process requires auxiliary factors that belong to the DCN1 (defective in cullin neddylation 1) family.
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History |
EC 2.3.2.32 created 2017
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Orthology |
K03868 | E3 ubiquitin-protein ligase RBX1 |
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Genes |
» show all
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Reference |
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Authors |
Kim AY, Bommelje CC, Lee BE, Yonekawa Y, Choi L, Morris LG, Huang G, Kaufman A, Ryan RJ, Hao B, Ramanathan Y, Singh B |
Title |
SCCRO (DCUN1D1) is an essential component of the E3 complex for neddylation. |
Journal |
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Reference |
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Authors |
Kurz T, Chou YC, Willems AR, Meyer-Schaller N, Hecht ML, Tyers M, Peter M, Sicheri F |
Title |
Dcn1 functions as a scaffold-type E3 ligase for cullin neddylation. |
Journal |
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Reference |
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Authors |
Scott DC, Monda JK, Grace CR, Duda DM, Kriwacki RW, Kurz T, Schulman BA |
Title |
A dual E3 mechanism for Rub1 ligation to Cdc53. |
Journal |
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Reference |
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Authors |
Scott DC, Sviderskiy VO, Monda JK, Lydeard JR, Cho SE, Harper JW, Schulman BA |
Title |
Structure of a RING E3 trapped in action reveals ligation mechanism for the ubiquitin-like protein NEDD8. |
Journal |
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Reference |
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Authors |
Monda JK, Scott DC, Miller DJ, Lydeard J, King D, Harper JW, Bennett EJ, Schulman BA |
Title |
Structural conservation of distinctive N-terminal acetylation-dependent interactions across a family of mammalian NEDD8 ligation enzymes. |
Journal |
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Other DBs |
ExplorEnz - The Enzyme Database: | 2.3.2.32 |
ExPASy - ENZYME nomenclature database: | 2.3.2.32 |
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