KEGG   ENZYME: 2.3.2.32
Entry
EC 2.3.2.32                 Enzyme                                 

Name
cullin-RING-type E3 NEDD8 transferase;
RBX1 (gene name)
Class
Transferases;
Acyltransferases;
Aminoacyltransferases
Sysname
[E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine:[cullin] [NEDD8-protein] transferase (isopeptide bond-forming; RING-type)
Reaction(IUBMB)
[E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-N6-[NEDD8-protein]-yl-L-lysine
Substrate
[E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine;
[cullin]-L-lysine
Product
[E2 NEDD8-conjugating enzyme]-L-cysteine;
[cullin]-N6-[NEDD8-protein]-yl-L-lysine
Comment
Some RING-type E3 ubiquitin transferase (EC 2.3.2.27) are not able to bind a substrate protein directly. Instead, they form a complex with a cullin scaffold protein and a substrate recognition module, which is named CRL for Cullin-RING-Ligase. The cullin protein needs to be activated by the ubiquitin-like protein NEDD8 in a process known as neddylation. The transfer of NEDD8 from a NEDD8-specific E2 enzyme onto the cullin protein is a secondary function of the RING-type E3 ubiquitin transferase in the CRL complex. The process requires auxiliary factors that belong to the DCN1 (defective in cullin neddylation 1) family.
History
EC 2.3.2.32 created 2017
Orthology
K03868  E3 ubiquitin-protein ligase RBX1
Genes
HSA: 9978(RBX1)
PTR: 458860(RBX1)
PPS: 100981636(RBX1)
GGO: 101151577(RBX1)
PON: 100441665(RBX1)
NLE: 100596079(RBX1)
MCC: 710252(RBX1)
MCF: 102132483(RBX1)
CSAB: 103223367(RBX1)
CATY: 105572541(RBX1)
RRO: 104673455(RBX1)
RBB: 108528465(RBX1)
TFN: 117096433(RBX1)
CJC: 100391523(RBX1)
SBQ: 101031903(RBX1)
MMUR: 105858549(RBX1)
MMU: 56438(Rbx1)
MCAL: 110310464(Rbx1)
MPAH: 110334961(Rbx1)
RNO: 300084(Rbx1)
MCOC: 116076358(Rbx1)
MUN: 110550435(Rbx1)
CGE: 100762556(Rbx1)
PLEU: 114694298(Rbx1) 114707186
NGI: 103743488(Rbx1)
HGL: 101712475(Rbx1)
CCAN: 109686914(Rbx1)
OCU: 103352470(RBX1)
TUP: 102468598(RBX1)
CFA: 474494(RBX1)
VVP: 112934005(RBX1)
UMR: 103674160(RBX1)
UAH: 113241739(RBX1)
ORO: 101363104(RBX1)
ELK: 111157501
MPUF: 101687674(RBX1)
EJU: 114218392(RBX1)
FCA: 101087758(RBX1)
PTG: 102955881(RBX1)
PPAD: 109265611(RBX1)
AJU: 113602716(RBX1)
BTA: 518880(RBX1) 780968
BOM: 102272719(RBX1)
BIU: 109559850(RBX1)
BBUB: 102414522(RBX1)
CHX: 102187112(RBX1) 102188771
OAS: 101109871(RBX1)
SSC: 110260638(RBX1)
CFR: 102515877(RBX1)
CDK: 105091911(RBX1)
BACU: 102998295(RBX1)
LVE: 103090829(RBX1)
OOR: 101287254(RBX1) 101289349
DLE: 111186303(RBX1)
PCAD: 102983946(RBX1)
ECB: 100055581(RBX1)
EPZ: 103556120(RBX1)
EAI: 106842455(RBX1)
MYD: 102760790(RBX1)
MNA: 107531277(RBX1)
HAI: 109392275(RBX1)
DRO: 112321770(RBX1)
SHON: 119002280(RBX1)
AJM: 119060371(RBX1)
MMF: 118618357 118624414(RBX1)
PALE: 102893437(RBX1)
RAY: 107519712(RBX1)
MJV: 108394317(RBX1)
LAV: 100657479(RBX1)
TMU: 101349359
MDO: 100013521(RBX1)
SHR: 100918404(RBX1)
PCW: 110205825(RBX1)
OAA: 100076928(RBX1)
GGA: 418001(RBX1)
MGP: 100543550(RBX1)
CJO: 107312930(RBX1)
NMEL: 110392307(RBX1)
APLA: 101796040(RBX1)
ACYG: 106035919(RBX1)
TGU: 100190092(RBX1)
LSR: 110483089(RBX1)
SCAN: 103813288(RBX1)
GFR: 102037823(RBX1)
FAB: 101806409(RBX1)
PHI: 102102832(RBX1)
PMAJ: 107204430(RBX1)
CCAE: 111934630(RBX1)
CCW: 104696532(RBX1)
ETL: 114058064(RBX1)
FPG: 101916874(RBX1)
FCH: 102049129(RBX1)
CLV: 102095842(RBX1)
ACUN: 113488055(RBX1)
PADL: 103915729(RBX1)
AAM: 106488968(RBX1)
ASN: 102372162(RBX1)
AMJ: 102571619(RBX1)
PSS: 102451113(RBX1)
CMY: 102943754(RBX1)
CPIC: 101936281(RBX1)
ACS: 100566553(rbx1)
PVT: 110076073(RBX1)
PBI: 103065423(RBX1)
PMUR: 107285413(RBX1)
TSR: 106537822(RBX1) 106553210
PMUA: 114605459(RBX1)
GJA: 107117140(RBX1)
XLA: 108714482(rbx1.L) 108715730(rbx1.S)
XTR: 100379935(rbx1)
NPR: 108788200(RBX1)
DRE: 449846(rbx1)
SANH: 107672143(rbx1) 107695112
SGH: 107580054 107602336(rbx1)
CCAR: 109076699(rbx1)
PHYP: 113524074(rbx1)
AMEX: 107197156(rbx1)
EEE: 113583718(rbx1)
LCO: 104932083(rbx1)
NCC: 104945083(rbx1)
MZE: 101480103(rbx1)
ONL: 100696705(rbx1)
OLA: 101170590(rbx1)
XMA: 102233269(rbx1)
XCO: 114160562(rbx1)
PRET: 103468459(rbx1)
CVG: 107093096(rbx1)
NFU: 107376330(rbx1)
KMR: 108236172(rbx1)
ALIM: 106520678(rbx1)
AOCE: 111570331(rbx1)
CSEM: 103393328(rbx1)
POV: 109643626(rbx1)
LCF: 108877581(rbx1)
SDU: 111230345(rbx1)
SLAL: 111645594 111647571(rbx1)
HCQ: 109514860(rbx1)
BPEC: 110162377(rbx1)
MALB: 109952664(rbx1)
SASA: 100136424(rbx1) 106601243(rbx1)
ELS: 105025789(rbx1)
PKI: 111845210(rbx1) 111853244
LCM: 102352187(RBX1)
CMK: 103189242(rbx1)
RTP: 109921792(rbx1)
BFO: 118419573
CIN: 100182516
APLC: 110984414
DME: Dmel_CG16982(Roc1a) Dmel_CG16988(Roc1b)
DSI: Dsimw501_GD11014(Dsim_GD11014) Dsimw501_GD16520(Dsim_GD16520)
AAG: 5565270
AME: 408691
BIM: 100750218
BTER: 100643234
CCAL: 108631453
OBB: 114871947
SOC: 105195164
MPHA: 105839647
AEC: 105148347
ACEP: 105626311
PBAR: 105432274
VEM: 105568857
HST: 105188519
DQU: 106740675
CFO: 105252239
LHU: 105674184
PGC: 109852514
OBO: 105284276
PCF: 106787165
NVI: 100115578
CSOL: 105361251
MDL: 103578781
TCA: 655935
DPA: 109536288
NVL: 108557707
BMOR: 692971
BMAN: 114248509
PMAC: 106708735
PRAP: 110992851
HAW: 110372277
TNL: 113508302
PXY: 105397966
DNX: 107162314
BTAB: 109044484
CLEC: 106666282
ZNE: 110832453
FCD: 110848901
PVM: 113818542
TUT: 107362230
DPTE: 113789362
PTEP: 107454093
CEL: CELE_ZK287.5(rbx-1)
CBR: CBG19342(Cbr-rbx-1)
BMY: Bm1_05985
PCAN: 112559116
CRG: 105317988
MYI: 110455961
OBI: 106882699
LAK: 106150763
NVE: 5509443
EPA: 110249442
ADF: 107349974
AMIL: 114969829
PDAM: 113677930
SPIS: 111341613
DGT: 114530307
HMG: 100204043
AQU: 100636037
ATH: AT3G42830 AT5G20570(RBX1)
ALY: 9308017
CPAP: 110817690
TCC: 18588272
LJA: Lj0g3v0042719.1(Lj0g3v0042719.1) Lj0g3v0262469.1(Lj0g3v0262469.1) Lj3g3v1339080.1(Lj3g3v1339080.1)
ZJU: 107423885
NNU: 104600540
DOSA: Os01t0106800-01(Os01g0106800) Os02t0708300-01(Os02g0708300)
PEQ: 110019661
CRE: CHLREDRAFT_79815(RBX1)
MNG: MNEG_4176
APRO: F751_6626
OLU: OSTLU_119493(rbx1)
MIS: MICPUN_113936(RBX1)
SCE: YOL133W(HRT1)
ERC: Ecym_2029
KMX: KLMA_70161(HRT1)
NCS: NCAS_0C04480(NCAS0C04480)
NDI: NDAI_0G03860(NDAI0G03860)
TPF: TPHA_0P01470(TPHA0P01470)
TBL: TBLA_0B08710(TBLA0B08710)
TDL: TDEL_0A00470(TDEL0A00470)
KAF: KAFR_0C01780(KAFR0C01780)
PIC: PICST_37145(HRT1)
CAL: CAALFM_C302450WA(HRT1)
NTE: NEUTE1DRAFT116888(NEUTE1DRAFT_116888)
MGR: MGG_08844
SSCK: SPSK_02774
MAW: MAC_01896
MAJ: MAA_00593
CMT: CCM_05836
BFU: BCIN_03g07500(Bchrt1)
MBE: MBM_08230
ANI: AN8844.2
ANG: ANI_1_294154(An17g02100)
ABE: ARB_07022
TVE: TRV_07648
PTE: PTT_16968
SPO: SPAC23H4.18c(rbx1)
CNE: CNB03140
CNB: CNBB2560
ABP: AGABI1DRAFT130185(AGABI1DRAFT_130185)
ABV: AGABI2DRAFT139777(AGABI2DRAFT_139777)
MGL: MGL_0788
MRT: MRET_0028
DDI: DDB_G0287629(rbx1)
EHI: EHI_151630(2.t00108)
PCB: PCHAS_080650(PC000269.01.0)
TAN: TA10565
TPV: TP04_0669
BBO: BBOV_III007590(17.m07664)
CPV: cgd8_930
SMIN: v1.2.005788.t1(symbB.v1.2.005788.t1)
PTI: PHATRDRAFT_39090(Rbx1)
NGD: NGA_0441600(RBX1)
SPAR: SPRG_11654
 » show all
Reference
1  [PMID:18826954]
  Authors
Kim AY, Bommelje CC, Lee BE, Yonekawa Y, Choi L, Morris LG, Huang G, Kaufman A, Ryan RJ, Hao B, Ramanathan Y, Singh B
  Title
SCCRO (DCUN1D1) is an essential component of the E3 complex for neddylation.
  Journal
J Biol Chem 283:33211-20 (2008)
DOI:10.1074/jbc.M804440200
Reference
2  [PMID:18206966]
  Authors
Kurz T, Chou YC, Willems AR, Meyer-Schaller N, Hecht ML, Tyers M, Peter M, Sicheri F
  Title
Dcn1 functions as a scaffold-type E3 ligase for cullin neddylation.
  Journal
Mol Cell 29:23-35 (2008)
DOI:10.1016/j.molcel.2007.12.012
Reference
3  [PMID:20832729]
  Authors
Scott DC, Monda JK, Grace CR, Duda DM, Kriwacki RW, Kurz T, Schulman BA
  Title
A dual E3 mechanism for Rub1 ligation to Cdc53.
  Journal
Mol Cell 39:784-96 (2010)
DOI:10.1016/j.molcel.2010.08.030
Reference
4  [PMID:24949976]
  Authors
Scott DC, Sviderskiy VO, Monda JK, Lydeard JR, Cho SE, Harper JW, Schulman BA
  Title
Structure of a RING E3 trapped in action reveals ligation mechanism for the ubiquitin-like protein NEDD8.
  Journal
Cell 157:1671-84 (2014)
DOI:10.1016/j.cell.2014.04.037
Reference
5  [PMID:23201271]
  Authors
Monda JK, Scott DC, Miller DJ, Lydeard J, King D, Harper JW, Bennett EJ, Schulman BA
  Title
Structural conservation of distinctive N-terminal acetylation-dependent interactions across a family of mammalian NEDD8 ligation enzymes.
  Journal
Structure 21:42-53 (2013)
DOI:10.1016/j.str.2012.10.013
Other DBs
ExplorEnz - The Enzyme Database: 2.3.2.32
IUBMB Enzyme Nomenclature: 2.3.2.32
ExPASy - ENZYME nomenclature database: 2.3.2.32
BRENDA, the Enzyme Database: 2.3.2.32

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