Entry
Name
RCR-type E3 ubiquitin transferase;
MYCBP2;
PHR1
Class
Transferases;
Acyltransferases;
Aminoacyltransferases
BRITE hierarchy
Sysname
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine:acceptor protein ubiquitin transferase (isopeptide bond-forming; RCR-type)
Reaction(IUBMB)
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-threonine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine (overall reaction);
(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RCR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RCR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine;
(1b) [RCR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-threonine = [RCR-type E3 ubiquitin transferase]-L-cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine
Substrate
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine;
[acceptor protein]-L-threonine;
[RCR-type E3 ubiquitin transferase]-L-cysteine;
[RCR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
Product
[E2 ubiquitin-conjugating enzyme]-L-cysteine;
[acceptor protein]-3-O-ubiquitinyl-L-threonine;
[RCR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine;
[RCR-type E3 ubiquitin transferase]-L-cysteine
Comment
RCR-type E3 ubiquitin transferases is a class of RING-type E3 ubiquitin transferase (see EC
2.3.2.27 ) that mediates ubiquitylation of acceptor proteins via an internal cysteine residue. The RING1 domain binds an EC
2.3.2.23 , E2 ubiquitin-conjugating enzyme, and transfers the ubiquitin that is bound to it to an internal cysteine residue on a mediator loop of the RCR-type ligase. The ubiquitin may be transferred to a second internal cysteine before the transfer of the ubiquitin from the RCR-type ligase to the substrate.
History
EC 2.3.2.33 created 2019
Orthology
K10693 RCR-type E3 ubiquitin transferase
Genes
CCAN : 109687459 109687461
DMER : 138811651 138814037(Mycbp2)
XLA : 108708496(mycbp2.L) 447587(mycbp2.S)
SRX : 107728238(mycbp2) 107749028
SANH : 107664005 107670894
CCAR : 109079135(mycbp2) 109095687
CAUA : 113053452 113109035(mycbp2)
CGIB : 127965571 128019527
GRF : 141301158 141340845(mycbp2)
MASI : 127446741 127447813
TNG : GSTEN00001239G001 GSTEN00027943G001
SASA : 100380858(mycb2) 106581613
STRU : 115156251(mycbp2) 115160590
OTW : 112225044(mycbp2) 112234653
OMY : 110501235(mycbp2) 110519948
OKI : 109870670(mycbp2) 109904047
OKE : 118367218 118385901(mycbp2)
OMM : 135519932 135547044(mycbp2)
OCLA : 139380072 139395705
SALP : 111959629(mycbp2) 111973092
CCLU : 121536600(mycbp2) 121554853
SFM : 108920533(mycbp2) 108929997
PSPA : 121320927(mycbp2) 121327729
ARUT : 117405836 117407076(mycbp2)
DSI : Dsimw501_GD15825(Dsim_GD15825)
ALUD : 128858449 128858453
AALB : 109427738 109431372
CQU : CpipJ_CPIJ003142 CpipJ_CPIJ003145
CPII : 120418076 120419869
CCAL : 108625172 108625982
ACEP : 105626860 105626861
CSOL : 105360333 105360334
DPL : KGM_203404 KGM_203405
PMAC : 106715875 106715876
BTAB : 109030725 109034485
PHU : Phum_PHUM483630 Phum_PHUM483780
CEL : CELE_C01B7.6(rpm-1) CELE_F07B7.12(rpms-1)
CBR : CBG_00759(Cbr-rpm-1)
LGI : LOTGIDRAFT_153260 LOTGIDRAFT_205644
MAEA : 128226745 128227140
SRE : PTSG_02092 PTSG_11546
» show all
Taxonomy
Reference
Authors
Pao KC, Wood NT, Knebel A, Rafie K, Stanley M, Mabbitt PD, Sundaramoorthy R, Hofmann K, van Aalten DMF, Virdee S
Title
Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.3.2.33
ExPASy - ENZYME nomenclature database: 2.3.2.33