KEGG   ENZYME: 2.3.3.18
Entry
EC 2.3.3.18                 Enzyme                                 

Name
2-phosphinomethylmalate synthase;
pmmS (gene name)
Class
Transferases;
Acyltransferases;
Acyl groups converted into alkyl groups on transfer
Sysname
acetyl-CoA:phosphinopyruvate C-acetyltransferase (thioester-hydrolysing, phosphinomethylmalate-forming)
Reaction(IUBMB)
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate = phosphinomethylmalate + CoA [RN:R08870]
Reaction(KEGG)
R08870
Substrate
acetyl-CoA [CPD:C00024];
H2O [CPD:C00001];
3-(hydroxyphosphinoyl)pyruvate
Product
phosphinomethylmalate;
CoA [CPD:C00010]
Comment
The enzyme, characterized from the bacterium Streptomyces hygroscopicus, participates in the pathway for bialaphos biosynthesis. It requires a divalent metal ion and can also act on oxaloacetate.
History
EC 2.3.3.18 created 2017
Pathway
ec00440  Phosphonate and phosphinate metabolism
ec01110  Biosynthesis of secondary metabolites
Orthology
K12910  2-phosphinomethylmalate synthase
Reference
1  [PMID:3170341]
  Authors
Shimotohno KW, Seto H, Otake N, Imai S, Murakami T
  Title
Studies on the biosynthesis of bialaphos (SF-1293). 8. Purification and characterization of 2-phosphinomethylmalic acid synthase from Streptomyces hygroscopicus SF-1293.
  Journal
J Antibiot (Tokyo) 41:1057-65 (1988)
DOI:10.7164/antibiotics.41.1057
Reference
2  [PMID:1368511]
  Authors
Shimotohno KW, Imai S, Murakami T, Seto H
  Title
Purification and characterization of citrate synthase from Streptomyces hygroscopicus SF-1293 and comparison of its properties with those of 2-phosphinomethylmalic acid synthase.
  Journal
Agric Biol Chem 54:463-70 (1990)
DOI:10.1271/bbb1961.54.463
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.3.3.18
IUBMB Enzyme Nomenclature: 2.3.3.18
ExPASy - ENZYME nomenclature database: 2.3.3.18
BRENDA, the Enzyme Database: 2.3.3.18

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