KEGG   ENZYME: 2.3.3.18Help
Entry
EC 2.3.3.18                 Enzyme                                 

Name
2-phosphinomethylmalate synthase;
pmmS (gene name)
Class
Transferases;
Acyltransferases;
Acyl groups converted into alkyl groups on transfer
BRITE hierarchy
Sysname
acetyl-CoA:phosphinopyruvate C-acetyltransferase (thioester-hydrolysing, phosphinomethylmalate-forming)
Reaction(IUBMB)
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate = phosphinomethylmalate + CoA [RN:R08870]
Reaction(KEGG)
Substrate
acetyl-CoA [CPD:C00024];
H2O [CPD:C00001];
3-(hydroxyphosphinoyl)pyruvate
Product
phosphinomethylmalate;
CoA [CPD:C00010]
Comment
The enzyme, characterized from the bacterium Streptomyces hygroscopicus, participates in the pathway for bialaphos biosynthesis. It requires a divalent metal ion and can also act on oxaloacetate.
History
EC 2.3.3.18 created 2017
Pathway
ec00440  Phosphonate and phosphinate metabolism
ec01110  Biosynthesis of secondary metabolites
ec01130  Biosynthesis of antibiotics
Orthology
K12910  2-phosphinomethylmalate synthase
Reference
1  [PMID:3170341]
  Authors
Shimotohno KW, Seto H, Otake N, Imai S, Murakami T
  Title
Studies on the biosynthesis of bialaphos (SF-1293). 8. Purification and characterization of 2-phosphinomethylmalic acid synthase from Streptomyces hygroscopicus SF-1293.
  Journal
J Antibiot (Tokyo) 41:1057-65 (1988)
Reference
2  [PMID:1368511]
  Authors
Shimotohno KW, Imai S, Murakami T, Seto H
  Title
Purification and characterization of citrate synthase from Streptomyces hygroscopicus SF-1293 and comparison of its properties with those of 2-phosphinomethylmalic acid synthase.
  Journal
Agric Biol Chem 54:463-70 (1990)
DOI:10.1271/bbb1961.54.463
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.3.3.18
IUBMB Enzyme Nomenclature: 2.3.3.18
ExPASy - ENZYME nomenclature database: 2.3.3.18
BRENDA, the Enzyme Database: 2.3.3.18

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