Entry
Name
alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase;
MGAT1 (gene name);
N-acetylglucosaminyltransferase I;
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I;
uridine diphosphoacetylglucosamine-alpha-1,3-mannosylglycoprotein beta-1,2-N-acetylglucosaminyltransferase;
UDP-N-acetylglucosaminyl:alpha-1,3-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase I;
UDP-N-acetylglucosaminyl:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I;
alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase;
GnTI;
GlcNAc-T I;
UDP-N-acetyl-D-glucosamine:3-(alpha-D-mannosyl)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-glucosamine:alpha-D-mannosyl-(1->3)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein] [RN:
R05983 ]
Reaction(KEGG)
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:
C00043 ];
Man5GlcNAc2-[protein]
Product
UDP [CPD:
C00015 ];
Man5GlcNAc3-[protein]
Comment
The enzyme, found in plants and animals, participates in the processing of N-glycans in the Golgi apparatus. Its action is required before the other N-acetylglucosaminyltransferases involved in the process (GlcNAcT-II through VI) can act. While the natural substrate (produced by EC
3.2.1.113 , mannosyl-oligosaccharide 1,2-alpha-mannosidase) is described here, the minimal substrate recognized by the enzyme is alpha-D-Man-(1->3)-beta-D-Man-R.
History
EC 2.4.1.101 created 1983, modified 2001 (EC 2.4.1.51 created 1972, part incorporated 1984), modified 2018
Pathway
ec00513 Various types of N-glycan biosynthesis
Orthology
K00726 alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Genes
MMUR : 105856688 105862231
MAUA : 121136501 121141311(Mgat1)
PLEU : 114681582 114706390(Mgat1)
MORG : 121456814 121463859
NCAR : 124960213 124965577 124987121
OAA : 100078839(MGAT1) 114808698
XLA : 379038(mgat1.S) 379047(mgat1.L)
BBUF : 120985012 120985025
DRE : 393649(mgat1a) 558524(mgat1b)
SRX : 107711329 107752174 107754829
SANH : 107677460 107694344 107704759
SGH : 107582291 107584341 107586870 107595592
CCAR : 109098409 109098410 109103367
CAUA : 113058617 113086534 113090776 113114979 113119527
CGIB : 127935643 127953782 127973089 127979004 128028955
PTET : 122323599(mgat1b) 122359477(mgat1a)
LROH : 127177322(mgat1a) 127181946(mgat1b)
OMC : 131521006(mgat1a) 131526158(mgat1b)
PPRM : 120477675(mgat1a) 120485209(mgat1b)
RKG : 130087923(mgat1b) 130104337(mgat1a)
MAMB : 125248867(mgat1a) 125274758(mgat1b)
CIDE : 127495472 127500512
CERY : 137001968(mgat1b) 137004943(mgat1a)
MASI : 127420974 127422332 127425462 127430176
TROS : 130551154 130564750(mgat1a) 130567355(mgat1b)
TDW : 130415952(mgat1b) 130428623(mgat1a)
MANU : 129438321(mgat1a) 129454535(mgat1b)
IPU : 108272752(mgat1b) 108278272(mgat1a)
IFU : 128615333(mgat1b) 128621436(mgat1a)
PHYP : 113539383 113543793
SMEO : 124381968(mgat1b) 124394720(mgat1a)
TFD : 113634768(mgat1b) 113642678(mgat1a)
TVC : 132842759(mgat1b) 132858147(mgat1a)
TRN : 134328704(mgat1b) 134334799(mgat1a)
AMEX : 103038529(mgat1a) 103041286(mgat1b)
CMAO : 118803856(mgat1a) 118805413(mgat1b)
TFS : 130518264(mgat1b) 130533172(mgat1a)
LCO : 104931064 104935037(mgat1)
TBEN : 117468572(mgat1a) 117477967(mgat1b)
CGOB : 115015791 115021878
PGEO : 117455038(mgat1b) 117461304(mgat1a)
GACU : 117539115(mgat1b) 117543177(mgat1a)
EMAC : 134861896(mgat1a) 134874660(mgat1b)
ELY : 117263573(mgat1b) 117265304(mgat1a)
EFO : 125893633(mgat1a) 125904271(mgat1b)
PLEP : 121939068 121945889(mgat1a) 121957923(mgat1b)
SLUC : 116035833(mgat1b) 116060010(mgat1a)
ECRA : 117946553(mgat1a) 117956584(mgat1b)
PFLV : 114557145(mgat1) 114568273
GAT : 120810986(mgat1a) 120826408(mgat1b)
PPUG : 119197606(mgat1a) 119218773(mgat1b)
AFB : 129097795(mgat1b) 129109795(mgat1a)
CLUM : 117728295(mgat1a) 117738864(mgat1b)
PSWI : 130187935(mgat1a) 130194725(mgat1b)
MSAM : 119897150 119897165 119907255(mgat1b)
SCHU : 122864721(mgat1b) 122881724(mgat1a)
CUD : 121513826(mgat1a) 121523901(mgat1b)
ALAT : 119006682(mgat1a) 119017022(mgat1b)
MZE : 101464977 101470803(mgat1)
ONL : 100704982(mgat1) 100711079
OAU : 116324360(mgat1b) 116331557(mgat1a)
OML : 112136629(mgat1a) 112160662(mgat1b)
CSAI : 133438439(mgat1a) 133460528(mgat1b)
XMA : 102224107 102224666(mgat1)
XCO : 114141545(mgat1) 114156531
XHE : 116716992(mgat1) 116731871
PRET : 103472139 103477744(mgat1)
PFOR : 103130700 103154658(mgat1)
PLAI : 106935006 106950208(mgat1)
PMEI : 106909632 106920417(mgat1)
GAF : 122831867(mgat1a) 122843985(mgat1b)
PPRL : 129352767(mgat1b) 129353518 129379966
CVG : 107081491 107103842 107105201(mgat1)
CTUL : 119784054(mgat1b) 119798284(mgat1a)
GMU : 124866409(mgat1a) 124879658(mgat1b)
NFU : 107379083(mgat1) 107383270
KMR : 108244446(mgat1b) 108249853(mgat1a)
ALIM : 106531005 106532624
NWH : 119409833(mgat1a) 119426141(mgat1b) 119429989
AOCE : 111579081(mgat1) 111585777
MCEP : 125017004(mgat1a) 125020384(mgat1b)
CSEM : 103388839 103394316
POV : 109631726 109646367(mgat1)
SSEN : 122774129(mgat1a) 122786262(mgat1b)
HHIP : 117771117(mgat1b) 117777159(mgat1a)
HSP : 118113759(mgat1a) 118124428(mgat1b)
PPLT : 128449670(mgat1a) 128461678(mgat1b)
SMAU : 118310415(mgat1a) 118311049(mgat1b)
LCF : 108896390(mgat1b) 108899572(mgat1a)
SDU : 111220846 111222600(mgat1)
SLAL : 111645605 111655642(mgat1)
XGL : 120784759(mgat1a) 120785896(mgat1b)
SSCV : 125975244 125986680
SBIA : 133501372(mgat1a) 133508860(mgat1b)
PEE : 133395994(mgat1b) 133402141(mgat1a)
PTAO : 133471169(mgat1b) 133479143(mgat1a)
BPEC : 110163468 110168068
MALB : 109952031 109974714(mgat1)
BSPL : 114843134(mgat1a) 114865256(mgat1b)
SJO : 128366542(mgat1a) 128374012(mgat1b)
SASA : 100196739(mgat1) 106570655 106582341 106589048
STRU : 115174120 115175317 115177027 115177645
OTW : 112229406 112230941(mgat1a) 112264307(mgat1b)
OMY : 110487870 110488311 110496720(mgat1b) 110516080(mgat1a)
OGO : 123997660 124020675(mgat1a) 124043147
ONE : 115104926 115117026 115128742 115141151
OKI : 109887254 109902098 109907130 109909205
OKE : 118364356 118367409(mgat1a) 118376056 118399665
SALP : 111959023 111965165 112068833
SNH : 120022760(mgat1b) 120027263 120031333 120047580
CCLU : 121553463(mgat1a) 121572241 121586828(mgat1b)
AANG : 118229264(mgat1a) 118233380(mgat1b)
PSPA : 121304354 121304389
DSI : Dsimw501_GD25269(Dsim_GD25269)
ASTE : 118509387 118517333
MQU : 128982213 128989916 128997855
SGRE : 126298102 126335725
DPX : DAPPUDRAFT_194495 DAPPUDRAFT_97601
RSAN : 119382534 119394545
DPTE : 113788596 113794712
LPOL : 106463385 106463514
CEL : CELE_B0416.6(gly-13) CELE_F48E3.1(gly-12) CELE_M01F1.1(gly-14)
CBR : CBG_09783(Cbr-gly-14) CBG_16168(Cbr-gly-13) CBG_16700(Cbr-gly-12)
CRQ : GCK72_009690 GCK72_023276 GCK72_024781
BMY : BM_BM4203(Bma-gly-12) BM_BM5050(Bm5050)
LOA : LOAG_01669 LOAG_06805 LOAG_08970
HRO : HELRODRAFT_72024 HELRODRAFT_85381
CVN : 111111013 111111259 111113415
PMAX : 117315404 117315405
OVI : T265_13020 T265_13021
ATEN : 116289352 116293696
AMIL : 114947271 114974165
PDAM : 113664252 113681548
SPIS : 111336517 111337738
CSAT : 104719898 104719899 104720738 104729134
HSYR : 120114367 120120335 120121814 120173830
MSYL : 126585664 126621301
PXB : 103926710 103926716 103932883 103933517
PALZ : 118032291 118038889
CILL : 122309223 122313559
NTA : 107789589(GntI) 107800221(gnTI) 107829720
SSPL : 121747318 121750195
ECAD : 122579771 122596686
CCAV : 112522664 112527204
CSIN : 114264780 114270794 114301638 114309099 114317479
PSOM : 113346831 113357026
TDC : 119270457 119278608 119318741 119325890
TAES : 123063454 123072464 123080684 123129239 123140150 123146386
MFLO : 136502691 136502710 136507902
SITA : 101782555 101786227
PVIR : 120655772 120702270
SMO : SELMODRAFT_268301(GntI)
APRO : F751_1686 F751_3216
MIS : MICPUN_55432 MICPUN_68567
CCP : CHC_T00008757001 CHC_T00009095001
EHX : EMIHUDRAFT_252190 EMIHUDRAFT_451362 EMIHUDRAFT_452664 EMIHUDRAFT_460566(GLT1)
NGR : NAEGRDRAFT_28878 NAEGRDRAFT_50501
» show all
Taxonomy
Reference
Authors
Harpaz N, Schachter H.
Title
Control of glycoprotein synthesis. Bovine colostrum UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase I. Separation from UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase II, partial purification, and substrate specificity.
Journal
J Biol Chem 255:4885-93 (1980)
Reference
Authors
Mendicino J, Chandrasekaran EV, Anumula KR, Davila M.
Title
Isolation and properties of alpha-D-mannose:beta-1,2-N-acetylglucosaminyltransferase from trachea mucosa.
Journal
Reference
Authors
Oppenheimer CL, Hill RL.
Title
Purification and characterization of a rabbit liver alpha 1 goes to 3 mannoside beta 1 goes to 2 N-acetylglucosaminyltransferase.
Journal
J Biol Chem 256:799-804 (1981)
Reference
Authors
Oppenheimer CL, Eckhardt AE, Hill RL.
Title
The nonidentity of porcine N-acetylglucosaminyltransferases I and II.
Journal
J Biol Chem 256:11477-82 (1981)
Reference
Authors
Miyagi T, Tsuiki S.
Title
Studies on UDP-N-acetylglucosamine : alpha-mannoside beta-N-acetylglucosaminyltransferase of rat liver and hepatomas.
Journal
Reference
Authors
Schachter H, Narasimhan S, Gleeson P, Vella G.
Title
Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type.
Journal
Reference
Authors
Vella GJ, Paulsen H, Schachter H.
Title
Control of glycoprotein synthesis. IX. A terminal Man alpha l-3Man beta 1- sequence in the substrate is the minimum requirement for UDP-N-acetyl-D-glucosamine: alpha-D-mannoside (GlcNAc to Man alpha 1-3) beta 2-N-acetylglucosaminyltransferase I.
Journal
Reference
Authors
Unligil UM, Zhou S, Yuwaraj S, Sarkar M, Schachter H, Rini JM
Title
X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: catalytic mechanism and a new protein superfamily.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 2.4.1.101