KEGG   ENZYME: 2.4.1.101
Entry
EC 2.4.1.101                Enzyme                                 

Name
alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase;
MGAT1 (gene name);
N-acetylglucosaminyltransferase I;
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I;
uridine diphosphoacetylglucosamine-alpha-1,3-mannosylglycoprotein beta-1,2-N-acetylglucosaminyltransferase;
UDP-N-acetylglucosaminyl:alpha-1,3-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase I;
UDP-N-acetylglucosaminyl:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I;
alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase;
GnTI;
GlcNAc-T I;
UDP-N-acetyl-D-glucosamine:3-(alpha-D-mannosyl)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-N-acetyl-alpha-D-glucosamine:alpha-D-mannosyl-(1->3)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein] [RN:R05983]
Reaction(KEGG)
R05983(G)
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
Man5GlcNAc2-[protein]
Product
UDP [CPD:C00015];
Man5GlcNAc3-[protein]
Comment
The enzyme, found in plants and animals, participates in the processing of N-glycans in the Golgi apparatus. Its action is required before the other N-acetylglucosaminyltransferases involved in the process (GlcNAcT-II through VI) can act. While the natural substrate (produced by EC 3.2.1.113, mannosyl-oligosaccharide 1,2-alpha-mannosidase) is described here, the minimal substrate recognized by the enzyme is alpha-D-Man-(1->3)-beta-D-Man-R.
History
EC 2.4.1.101 created 1983, modified 2001 (EC 2.4.1.51 created 1972, part incorporated 1984), modified 2018
Pathway
ec00510  N-Glycan biosynthesis
ec00513  Various types of N-glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K00726  alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Genes
HSA: 4245(MGAT1)
PTR: 462340(MGAT1)
PPS: 100992340(MGAT1)
GGO: 101154311(MGAT1)
PON: 100172524(MGAT1)
NLE: 100583691(MGAT1)
MCC: 707375(MGAT1)
MCF: 102123962(MGAT1)
CSAB: 103245131(MGAT1)
RRO: 104671520(MGAT1)
RBB: 108514170(MGAT1)
CJC: 100398071(MGAT1)
SBQ: 101027856(MGAT1)
MMU: 17308(Mgat1)
MCAL: 110305339(Mgat1)
MPAH: 110332580(Mgat1)
RNO: 81519(Mgat1)
MUN: 110551024(Mgat1)
CGE: 100682529(Mgat1)
NGI: 103744235(Mgat1)
HGL: 101725493(Mgat1)
CCAN: 109695526(Mgat1)
OCU: 100009521(MGAT1)
TUP: 102480807(MGAT1)
CFA: 491674(MGAT1)
VVP: 112912215(MGAT1)
AML: 100484226(MGAT1)
UMR: 103656381(MGAT1)
UAH: 113261524(MGAT1)
ORO: 101375424(MGAT1)
ELK: 111139634
FCA: 101082035(MGAT1)
PTG: 102954826(MGAT1)
PPAD: 109260647(MGAT1)
AJU: 106988093(MGAT1)
BTA: 534248(MGAT1)
BOM: 102270151(MGAT1)
BBUB: 102408945(MGAT1)
CHX: 102181772(MGAT1)
OAS: 101104744(MGAT1)
SSC: 780412(MGAT1)
CFR: 102518411(MGAT1)
CDK: 105099912(MGAT1)
BACU: 103001085(MGAT1)
LVE: 103084987(MGAT1)
OOR: 101280084(MGAT1)
DLE: 111166692(MGAT1)
PCAD: 102996609(MGAT1)
ECB: 102149649(MGAT1)
EPZ: 103541218(MGAT1)
EAI: 106825529(MGAT1)
MYB: 102262787(MGAT1)
MYD: 102753141(MGAT1)
MNA: 107528438(MGAT1)
HAI: 109392782(MGAT1)
DRO: 112296407(MGAT1)
PALE: 102889186(MGAT1)
RAY: 107512333(MGAT1)
MJV: 108390701(MGAT1)
LAV: 100676752(MGAT1)
TMU: 101353600
MDO: 100019068(MGAT1)
SHR: 100930793(MGAT1)
PCW: 110202637(MGAT1)
OAA: 100078839(MGAT1) 114808698
GGA: 112530444(MGAT1)
MGP: 104916124(MGAT1)
CJO: 107307501(MGAT1)
NMEL: 110390854(MGAT1)
ACYG: 106029342(MGAT1)
TGU: 100223060(MGAT1)
LSR: 110482925(MGAT1)
SCAN: 103825265(MGAT1)
FAB: 101815447(MGAT1)
PHI: 102107929(MGAT1)
PMAJ: 107199580(MGAT1)
CCAE: 111943883(MGAT1)
FPG: 101921665(MGAT1)
FCH: 102051009(MGAT1)
CLV: 102084563(MGAT1)
EGZ: 104124465(MGAT1)
NNI: 104011857(MGAT1)
ASN: 102373122(MGAT1)
AMJ: 102566676(MGAT1)
PSS: 102463624(MGAT1)
CMY: 102938441
CPIC: 101944629(MGAT1)
ACS: 100559184(mgat1)
PVT: 110091186(MGAT1)
PBI: 103062061(MGAT1)
PMUR: 107289640(MGAT1)
TSR: 106553519(MGAT1)
PMUA: 114588275(MGAT1)
GJA: 107121961(MGAT1)
XLA: 100337572(glcnac) 379038(mgat1.S) 379047(MGC52514)
XTR: 496815(mgat1)
NPR: 108793226(MGAT1)
DRE: 393649(mgat1a) 558524(mgat1b)
IPU: 108272752(MGAT1) 108278272
LCO: 104931064 104935037(mgat1)
MZE: 101464977 101470803(mgat1)
ONL: 100704982(mgat1) 100711079
XMA: 102224107 102224666(mgat1)
XCO: 114141545(mgat1) 114156531
PRET: 103472139 103477744(mgat1)
NFU: 107379083(mgat1) 107383270
AOCE: 111579081(mgat1) 111585777
POV: 109631726 109646367(mgat1)
LCF: 108896390 108899572(mgat1)
SDU: 111220846 111222600(mgat1)
SLAL: 111645605 111655642(mgat1)
MALB: 109952031 109974714(mgat1)
SFM: 108935639(mgat1)
PKI: 111842828(mgat1)
LCM: 102366347(MGAT1)
CMK: 103191271(mgat1)
RTP: 109922664(mgat1)
CIN: 100179884
SPU: 594485
APLC: 110984518
SKO: 100370369
DME: Dmel_CG13431(Mgat1)
DER: 6547472
DSE: 6610088
DSI: Dsimw501_GD25269(Dsim_GD25269)
DAN: 6495607
DSR: 110182931
DPE: 6591024
DWI: 6639911
DAZ: 108616949
DNV: 108654575
DHE: 111600729
DVI: 6626831
MDE: 101890826
LCQ: 111677803
AAG: 5574341
AALB: 109399522
AME: 410105
BIM: 100740866
BTER: 100644833
CCAL: 108630178
OBB: 114872169
SOC: 105198596
MPHA: 105831741
AEC: 105145646
ACEP: 105626038
PBAR: 105427478
VEM: 105567882
HST: 105181739
DQU: 106744521
CFO: 105257572
LHU: 105669177
PGC: 109852939
OBO: 105283181
PCF: 106792249
NVI: 103317648
CSOL: 105366822
MDL: 103578580
TCA: 662130
DPA: 109540499
ATD: 109597350
NVL: 108556813
BMOR: 100653407(gly)
BMAN: 114241912
PMAC: 106715225
PRAP: 110995340
HAW: 110384682
TNL: 113504095
API: 100160307
DNX: 107171927
AGS: 114129770
RMD: 113551811
BTAB: 109039465
CLEC: 106664368
ZNE: 110836147
FCD: 110849419
TUT: 107368563
CSCU: 111628216
PTEP: 107439807
CEL: CELE_B0416.6(gly-13) CELE_F48E3.1(gly-12) CELE_M01F1.1(gly-14)
CBR: CBG09783(Cbr-gly-14) CBG16168(Cbr-gly-13) CBG16700(Cbr-gly-12)
BMY: Bm1_21155
TSP: Tsp_09761
PCAN: 112557510
CRG: 105346421
OBI: 106874412
LAK: 106177004
SHX: MS3_06704
EGL: EGR_07167
NVE: 5514111
ADF: 107340776
DGT: 114525704
HMG: 100210187
AQU: 100631766
ATH: AT4G38240(CGL1)
ALY: 9302862
CRB: 17878215
BRP: 103828113
BOE: 106303510
THJ: 104824732
CPAP: 110825649
CIT: 102613005
TCC: 18608364
GRA: 105762705
GAB: 108479923
VRA: 106762020
VAR: 108331575
VUN: 114162318
CCAJ: 109816934
CAM: 101510931
LJA: Lj1g3v3329910.1(Lj1g3v3329910.1) Lj1g3v3329910.2(Lj1g3v3329910.2)
ADU: 107483412
AIP: 107624822
LANG: 109328357
FVE: 101302314
RCN: 112174416
PPER: 18766837
PMUM: 103334615
PAVI: 110754708
ZJU: 107427172
CSV: 101218473
CMO: 103490207
MCHA: 111007669
CMAX: 111468191
CMOS: 111454925
CPEP: 111811703
RCU: 8281446
JCU: 105635431
HBR: 110672196
MESC: 110626342
JRE: 109006658
QSU: 112010582
VVI: 100243059
SLY: 101257812
SPEN: 107008139
SOT: 102596963(GntI)
CANN: 107840650
NTA: 107789589(GntI) 107800221(gnTI) 107829720
NTO: 104102713
NAU: 109243852
INI: 109161530
SIND: 105169864
OEU: 111410510
DCR: 108196690
BVG: 104904067
SOE: 110793324
CQI: 110681815
NNU: 104606818
OSA: 4331263
DOSA: Os02t0832800-01(Os02g0832800)
OBR: 102714347
BDI: 100835640
ATS: 109735716(LOC109735716) 109752693(LOC109752693)
SBI: 8073458
ZMA: 100274447(si945027c08(99))
MUS: 104000486
AOF: 109848312
ATR: 18445514
SMO: SELMODRAFT_268301(GntI)
PPP: 112275318
DDI: DDB_G0288463(gnt3)
DFA: DFA_02421(gnt3)
SMIN: v1.2.003563.t1(symbB.v1.2.003563.t1) v1.2.015897.t1(symbB.v1.2.015897.t1) v1.2.015897.t2(symbB.v1.2.015897.t2) v1.2.037358.t1(symbB.v1.2.037358.t1) v1.2.040735.t1(symbB.v1.2.040735.t1)
 » show all
Reference
1  [PMID:6445358]
  Authors
Harpaz N, Schachter H.
  Title
Control of glycoprotein synthesis. Bovine colostrum UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase I. Separation from UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase II, partial purification, and substrate specificity.
  Journal
J Biol Chem 255:4885-93 (1980)
Reference
2  [PMID:6452163]
  Authors
Mendicino J, Chandrasekaran EV, Anumula KR, Davila M.
  Title
Isolation and properties of alpha-D-mannose:beta-1,2-N-acetylglucosaminyltransferase from trachea mucosa.
  Journal
Biochemistry 20:967-76 (1981)
DOI:10.1021/bi00507a050
Reference
3  [PMID:6450208]
  Authors
Oppenheimer CL, Hill RL.
  Title
Purification and characterization of a rabbit liver alpha 1 goes to 3 mannoside beta 1 goes to 2 N-acetylglucosaminyltransferase.
  Journal
J Biol Chem 256:799-804 (1981)
Reference
4  [PMID:6457827]
  Authors
Oppenheimer CL, Eckhardt AE, Hill RL.
  Title
The nonidentity of porcine N-acetylglucosaminyltransferases I and II.
  Journal
J Biol Chem 256:11477-82 (1981)
Reference
5  [PMID:6170335]
  Authors
Miyagi T, Tsuiki S.
  Title
Studies on UDP-N-acetylglucosamine : alpha-mannoside beta-N-acetylglucosaminyltransferase of rat liver and hepatomas.
  Journal
Biochim Biophys Acta 661:148-57 (1981)
DOI:10.1016/0005-2744(81)90094-2
Reference
6  [PMID:6366476]
  Authors
Schachter H, Narasimhan S, Gleeson P, Vella G.
  Title
Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type.
  Journal
Methods Enzymol 98:98-134 (1983)
DOI:10.1016/0076-6879(83)98143-0
Reference
7  [PMID:6235906]
  Authors
Vella GJ, Paulsen H, Schachter H.
  Title
Control of glycoprotein synthesis. IX. A terminal Man alpha l-3Man beta 1- sequence in the substrate is the minimum requirement for UDP-N-acetyl-D-glucosamine: alpha-D-mannoside (GlcNAc to Man alpha 1-3) beta 2-N-acetylglucosaminyltransferase I.
  Journal
Can J Biochem Cell Biol 62:409-17 (1984)
DOI:10.1139/o84-056
Reference
8  [PMID:11032794]
  Authors
Unligil UM, Zhou S, Yuwaraj S, Sarkar M, Schachter H, Rini JM.
  Title
X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: catalytic mechanism and a new protein superfamily.
  Journal
EMBO J 19:5269-80 (2000)
DOI:10.1093/emboj/19.20.5269
  Sequence
[ocu:100009521]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.101
IUBMB Enzyme Nomenclature: 2.4.1.101
ExPASy - ENZYME nomenclature database: 2.4.1.101
BRENDA, the Enzyme Database: 2.4.1.101
CAS: 102576-81-8

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