KEGG   ENZYME: 2.4.1.101
Entry
EC 2.4.1.101                Enzyme                                 
Name
alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase;
MGAT1 (gene name);
N-acetylglucosaminyltransferase I;
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I;
uridine diphosphoacetylglucosamine-alpha-1,3-mannosylglycoprotein beta-1,2-N-acetylglucosaminyltransferase;
UDP-N-acetylglucosaminyl:alpha-1,3-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase I;
UDP-N-acetylglucosaminyl:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I;
alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase;
GnTI;
GlcNAc-T I;
UDP-N-acetyl-D-glucosamine:3-(alpha-D-mannosyl)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-N-acetyl-alpha-D-glucosamine:alpha-D-mannosyl-(1->3)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein] [RN:R05983]
Reaction(KEGG)
R05983(G)
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
Man5GlcNAc2-[protein]
Product
UDP [CPD:C00015];
Man5GlcNAc3-[protein]
Comment
The enzyme, found in plants and animals, participates in the processing of N-glycans in the Golgi apparatus. Its action is required before the other N-acetylglucosaminyltransferases involved in the process (GlcNAcT-II through VI) can act. While the natural substrate (produced by EC 3.2.1.113, mannosyl-oligosaccharide 1,2-alpha-mannosidase) is described here, the minimal substrate recognized by the enzyme is alpha-D-Man-(1->3)-beta-D-Man-R.
History
EC 2.4.1.101 created 1983, modified 2001 (EC 2.4.1.51 created 1972, part incorporated 1984), modified 2018
Pathway
ec00510  N-Glycan biosynthesis
ec00513  Various types of N-glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K00726  alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Genes
HSA4245(MGAT1)
PTR462340(MGAT1)
PPS100992340(MGAT1)
GGO101154311(MGAT1)
PON100172524(MGAT1)
NLE100583691(MGAT1)
MCC707375(MGAT1)
MCF102123962(MGAT1)
CSAB103245131(MGAT1)
CATY105590428(MGAT1)
PANU103885650(MGAT1)
TGE112625714(MGAT1)
RRO104671520(MGAT1)
RBB108514170(MGAT1)
TFN117064802(MGAT1)
PTEH111527324(MGAT1)
CJC100398071(MGAT1)
SBQ101027856(MGAT1)
MMUR105856688 105862231
OGA100948255(MGAT1)
MMU17308(Mgat1)
MCAL110305339(Mgat1)
MPAH110332580(Mgat1)
RNO81519(Mgat1)
MCOC116077261(Mgat1)
MUN110551024(Mgat1)
CGE100682529(Mgat1)
PLEU114681582 114706390(Mgat1)
NGI103744235(Mgat1)
HGL101725493(Mgat1)
CPOC100733330(Mgat1)
CCAN109695526(Mgat1)
DSP122115414(Mgat1)
NCAR124960213 124965577 124987121
OCU100009521(MGAT1)
OPI101523848(MGAT1)
TUP102480807(MGAT1)
CFA491674(MGAT1)
VVP112912215(MGAT1)
VLG121494938(MGAT1)
AML100484226(MGAT1)
UMR103656381(MGAT1)
UAH113261524(MGAT1)
UAR123780218(MGAT1)
ELK111139634
LLV125100560
MPUF101693464(MGAT1)
ORO101375424(MGAT1)
EJU114200251(MGAT1)
ZCA113928948(MGAT1)
MLX118026533(MGAT1)
FCA101082035(MGAT1)
PYU121016650(MGAT1)
PBG122485703(MGAT1)
PTG102954826(MGAT1)
PPAD109260647(MGAT1)
AJU106988093(MGAT1)
HHV120226825(MGAT1)
BTA534248(MGAT1)
BOM102270151(MGAT1)
BIU109561934 109561946
BBUB102408945(MGAT1)
CHX102181772(MGAT1)
OAS101104744(MGAT1)
ODA120863823(MGAT1)
CCAD122439994(MGAT1)
SSC780412(MGAT1)
CFR102518411(MGAT1)
CBAI105065228(MGAT1)
CDK105099912(MGAT1)
VPC102525390(MGAT1)
BACU103001085(MGAT1)
LVE103084987(MGAT1)
OOR101280084(MGAT1)
DLE111166692(MGAT1)
PCAD102996609(MGAT1)
PSIU116751773(MGAT1)
ECB102149649(MGAT1)
EPZ103541218(MGAT1)
EAI106825529(MGAT1)
MYB102262787(MGAT1)
MYD102753141(MGAT1)
MMYO118658177(MGAT1)
MLF102427935(MGAT1)
MNA107528438(MGAT1)
PKL118725938(MGAT1)
HAI109392782(MGAT1)
DRO112296407(MGAT1)
SHON118995108(MGAT1)
AJM119042600(MGAT1)
PDIC114509619(MGAT1)
PHAS123808956(MGAT1)
MMF118615599(MGAT1)
RFQ117016653(MGAT1)
PALE102889186(MGAT1)
PGIG120616608(MGAT1)
PVP105299981(MGAT1)
RAY107512333(MGAT1)
MJV108390701(MGAT1)
TOD119235216(MGAT1)
SARA101557995(MGAT1)
LAV100676752(MGAT1)
TMU101353600
DNM101419091(MGAT1)
MDO100019068(MGAT1)
GAS123244900(MGAT1)
SHR100930793(MGAT1)
PCW110202637(MGAT1)
OAA100078839(MGAT1) 114808698
GGA112530444(MGAT1)
PCOC116240998(MGAT1)
MGP104916124(MGAT1)
CJO107307501(MGAT1)
NMEL110390854(MGAT1)
ACYG106029342(MGAT1)
AFUL116500276(MGAT1)
TGU100223060(MGAT1)
LSR110482925(MGAT1)
SCAN103825265(MGAT1)
PMOA120506972(MGAT1)
OTC121341063(MGAT1)
PRUF121348644(MGAT1)
FAB101815447(MGAT1)
PHI102107929(MGAT1)
PMAJ107199580(MGAT1)
CCAE111943883(MGAT1)
FPG101921665(MGAT1)
FCH102051009(MGAT1)
CLV102084563(MGAT1)
EGZ104124465(MGAT1)
NNI104011857(MGAT1)
TALA116960912(MGAT1)
ACHC115337871(MGAT1)
AROW112964952(MGAT1)
DNE112992951(MGAT1)
ASN102373122(MGAT1)
AMJ102566676(MGAT1)
PSS102463624(MGAT1)
CMY102938441(MGAT1)
CPIC101944629(MGAT1)
CABI116836417(MGAT1)
MRV120382899(MGAT1)
ACS100559184(mgat1)
PVT110091186(MGAT1)
SUND121923992(MGAT1)
PBI103062061(MGAT1)
PMUR107289640(MGAT1)
TSR106553519(MGAT1)
PGUT117668004(MGAT1)
VKO123034434(MGAT1)
PMUA114588275(MGAT1)
ZVI118080951(MGAT1)
GJA107121961(MGAT1)
STOW125430175(MGAT1)
XLA379038(mgat1.S) 379047(mgat1.L)
XTR496815(mgat1)
NPR108793226(MGAT1)
RTEM120914339(MGAT1)
BBUF120985012 120985025
BGAR122946063(MGAT1)
DRE393649(mgat1a) 558524(mgat1b)
SRX107711329 107752174 107754829
SANH107677460 107694344 107704759
SGH107582291 107584341 107586870 107595592
CCAR109098409 109098410 109103367
CAUA113058617 113086534 113090776 113114979 113119527
PPRM120477675(mgat1a) 120485209(mgat1b)
MAMB125248867(mgat1a) 125274758(mgat1b)
IPU108272752(mgat1b) 108278272(mgat1a)
PHYP113539383 113543793
SMEO124381968(mgat1b) 124394720(mgat1a)
TFD113634768(mgat1b) 113642678(mgat1a)
AMEX103038529 103041286
EEE113586397 113590323
TRU101073419 101076647
TNGGSTEN00021926G001
LCO104931064 104935037(mgat1)
NCC104942243 104964845
CGOB115015791 115021878
ELY117263573(mgat1b) 117265304(mgat1a)
PLEP121939068 121945889(mgat1a) 121957923(mgat1b)
SLUC116035833(mgat1b) 116060010(mgat1a)
ECRA117946553(mgat1a) 117956584(mgat1b)
PFLV114557145(mgat1) 114568273
GAT120810986(mgat1a) 120826408(mgat1b)
PPUG119197606(mgat1a) 119218773(mgat1b)
MSAM119897150 119897165 119907255(mgat1b)
CUD121513826(mgat1a) 121523901(mgat1b)
MZE101464977 101470803(mgat1)
ONL100704982(mgat1) 100711079
OAU116324360(mgat1b) 116331557(mgat1a)
OLA101169389 101173236
OML112136629(mgat1a) 112160662(mgat1b)
XMA102224107 102224666(mgat1)
XCO114141545(mgat1) 114156531
XHE116716992(mgat1) 116731871
PRET103472139 103477744(mgat1)
PFOR103130700 103154658(mgat1)
PLAI106935006 106950208(mgat1)
PMEI106909632 106920417(mgat1)
GAF122831867(mgat1a) 122843985(mgat1b)
CVG107081491 107103842 107105201(mgat1)
CTUL119784054(mgat1b) 119798284(mgat1a)
GMU124866409(mgat1a) 124879658(mgat1b)
NFU107379083(mgat1) 107383270
KMR108244446(mgat1b) 108249853(mgat1a)
ALIM106531005 106532624
NWH119409833(mgat1a) 119426141(mgat1b) 119429989
AOCE111579081(mgat1) 111585777
CSEM103388839 103394316
POV109631726 109646367(mgat1)
SSEN122774129(mgat1a) 122786262(mgat1b)
HHIP117771117(mgat1b) 117777159(mgat1a)
LCF108896390 108899572(mgat1)
SDU111220846 111222600(mgat1)
SLAL111645605 111655642(mgat1)
XGL120784759(mgat1a) 120785896(mgat1b)
HCQ109521014 109528061
BPEC110163468 110168068
MALB109952031 109974714(mgat1)
SASA100196739(mgat1) 106570655 106582341 106589048
OTW112229406 112230941(mgat1a) 112264307(mgat1b)
OMY110487870 110488311 110496720(mgat1b) 110516080(mgat1a)
OGO123997660 124020675(mgat1a) 124043147
ONE115104926 115117026 115128742 115141151
SALP111959023 111965165 112068833
SNH120022760(mgat1b) 120027263 120031333 120047580
ELS105012334 105031351
SFM108935639(mgat1)
PKI111842828(mgat1)
AANG118229264(mgat1a) 118233380(mgat1b)
PSPA121304354 121304389
ARUT117433963(mgat1b)
LCM102366347(MGAT1)
CMK103191271(mgat1b)
RTP109922664(mgat1)
BFO118412189 118416423
BBEL109468727
CIN100179884
SCLV120344133
SPU594485
APLC110984518
SKO100370369
DMEDmel_CG13431(Mgat1)
DER6547472
DSE6610088
DSIDsimw501_GD25269(Dsim_GD25269)
DYADyak_GE13790
DAN6495607
DSR110182931
DPODpse_GA12283
DPE6591024
DMN108160443 117193319
DWI6639911
DGR6559332
DMODmoj_GI19251
DAZ108616949
DNV108654575
DHE111600729
DVI6626831
CCAT101457557
BOD106622702
MDE101890826
SCAC106095035
LCQ111677803
AGAAgaP_AGAP005347
ACOZ120950105
AAG5574341
AALB109399522
CQUCpipJ_CPIJ003929
CPII120423785
CNS116351915
AME410105
ACER107997071
ALAB122711393
BIM100740866
BBIF117214223
BVK117242313
BVAN117161083
BTER100644833
BPYO122576666
CCAL108630178
OBB114872169
MGEN117226801
NMEA116433046
CGIG122402497
SOC105198596
MPHA105831741
AEC105145646
ACEP105626038
PBAR105427478
VEM105567882
HST105181739
DQU106744521
CFO105257572
FEX115242079
LHU105669177
PGC109852939
OBO105283181
PCF106792249
PFUC122517929
VPS122629162
NVI103317648
CSOL105366822
TPRE106653196
MDL103578580
CGLO123268773
FAS105263375
DAM107043722
AGIF122849793
CCIN107272069
TCA662130
DPA109540499
SOY115882906
ATD109597350
CSET123308734
AGB108914666
LDC111513142
NVL108556813
APLN108738452
PPYR116163112
OTU111420907
BMOR100653407(gly)
BMAN114241912
MSEX115441585
DPLKGM_206745B
BANY112055247
PMAC106715225
PPOT106101823
PXU106124321
PRAP110995340
ZCE119836521
HAW110384682
TNL113504095
SLIU111350102
OFU114350279
PXY105381303 105385113
API100160307
DNX107171927
AGS114129770
RMD113551811
BTAB109039465
DCI103514339 103523282
CLEC106664368
HHAL106685214
NLU111047424
PHUPhum_PHUM374380
FOC113213041
ZNE110836147
CSEC111874738
FCD110849419
DPXDAPPUDRAFT_194495 DAPPUDRAFT_97601
DMK116929643
PVM113807916 113812787
PJA122259959
PCHN125043509
HAME121859142
PCLA123757837
PTRU123515103
HAZT108668471
EAF111699950
LSM121121104
ISCIscW_ISCW009302 IscW_ISCW012048
DSV119455356
RSAN119382534 119394545
RMP119172495 119176778
VDE111248188 111251941
VJA111269312 111272967
TUT107368563
DPTE113788596 113794712
DFR124494193 124499449
CSCU111628216
PTEP107439807
SDM118198665 118199157
CELCELE_B0416.6(gly-13) CELE_F48E3.1(gly-12) CELE_M01F1.1(gly-14)
CBRCBG_09783(Cbr-gly-14) CBG_16168(Cbr-gly-13) CBG_16700(Cbr-gly-12)
BMYBM_BM4203(Bma-gly-12) BM_BM5050(Bm5050)
LOALOAG_01669 LOAG_06805 LOAG_08970
NAINECAME_05151
TSPTsp_09761
HROHELRODRAFT_72024 HELRODRAFT_85381
LGILOTGIDRAFT_217488
PCAN112557510
BGT106069718
GAE121373484
HRF124151631
HRJ124259229
CRG105346421
MYI110446576 110446577
PMAX117315404 117315405
MMER123542746
OBI106874412
OSN115219184
LAK106177004
SMMSmp_026930
SHXMS3_0013388
OVIT265_13020 T265_13021
EGLEGR_07167
NVE5514111
EPA110232315 110252394
ATEN116289352 116293696
ADF107340776
AMIL114947271 114974165
PDAM113664252 113681548
SPIS111336517 111337738
DGT114525704
XEN124452973
HMG100210187
TADTRIADDRAFT_19831
AQU100631766
ATHAT4G38240(CGL1)
ALY9302862
CRB17878215
CSAT104719898 104719899 104720738 104729134
EUSEUTSA_v10025208mg
BRP103828113
BNA106433266 106435542
BOE106303510
RSZ108854795 108855090
THJ104824732
CPAP110825649
CIT102613005
CICCICLE_v100284181m
PVY116141336
MINC123226889
TCC18608364
GRA105762705
GHI107929289 107929602
GAB108479923
DZI111294990 111309143
EGR104415910 104415914 104428625
GMX100776378 100795005
GSJ114408361 114414388
PVUPHAVU_009G115600g
VRA106762020
VAR108331575
VUN114162318
CCAJ109816934
APRC113866010
MTRMTR_3g118520
CAM101510931
LJALj1g3v3329910.1(Lj1g3v3329910.1) Lj1g3v3329910.2(Lj1g3v3329910.2)
ADU107483412
AIP107624822
AHF112720243 112790155
LANG109328357
FVE101302314
RCN112174416
PPER18766837
PMUM103334615
PAVI110754708
PDUL117637651
MDM103422182 103440937
PXB103926710 103926716 103932883 103933517
ZJU107427172
MNT21396885
CSV101218473
CMO103490207
BHJ120078073
MCHA111007669
CMAX111468191
CMOS111454925
CPEP111811703
RCU8281446
JCU105635431
HBR110672196
MESC110626342
POP18098157 7474891
PEU105108312 105136978
PALZ118032291 118038889
JRE109006658
QSU112010582
QLO115969117
TWL119991048
VVI100243059
VRI117918900
SLY101257812
SPEN107008139
SOT102596963(GntI)
CANN107840650
NTA107789589(GntI) 107800221(gnTI) 107829720
NSY104224802 104247634
NTO104102713
NAU109243852
INI109161530
ITR116030081
SIND105169864
OEU111410510
EGT105957836
SSPL121747318 121750195
HAN110885392 110911371
ECAD122579771 122596686
LSV111888342 111899671
CCAV112522664 112527204
DCR108196690
CSIN114264780 114270794 114301638 114309099 114317479
BVG104904067
SOE110793324
CQI110681815
NNU104606818
MING122085204
TSS122659947
PSOM113346831 113357026
NCOL116260981
OSA4331263
DOSAOs02t0832800-01(Os02g0832800)
OBR102714347
BDI100835640
ATS109735716 109752693
TDC119270457 119278608 119318741 119325890
TAES123063454 123072464 123080684 123129239 123140150 123146386
SBI8073458
ZMA100274447
SITA101782555 101786227
SVS117852742
PVIR120655772 120702270
PHAI112888575
PDA103710476 103723991
EGU105055246 105056496
MUS104000486
DCT110100408 110114943
PEQ110025694 110039002
AOF109848312
ATR18445514
SMOSELMODRAFT_268301(GntI)
PPP112275318
CSLCOCSUDRAFT_3892
CVRCHLNCDRAFT_8691
APROF751_1686 F751_3216
MISMICPUN_55432 MICPUN_68567
MPPMICPUCDRAFT_32201
GSLGasu_02010
CCPCHC_T00008757001 CHC_T00009095001
MBRMONBRDRAFT_28515
SREPTSG_00299
DDIDDB_G0288463(gnt3)
DPPDICPUDRAFT_88972
DFADFA_02421(gnt3)
ACANACA1_041910
SMINv1.2.003563.t1(symbB.v1.2.003563.t1) v1.2.015897.t1(symbB.v1.2.015897.t1) v1.2.015897.t2(symbB.v1.2.015897.t2) v1.2.037358.t1(symbB.v1.2.037358.t1) v1.2.040735.t1(symbB.v1.2.040735.t1)
PTIPHATRDRAFT_54844
FCYFRACYDRAFT_189180
TPSTHAPSDRAFT_1600
EHXEMIHUDRAFT_252190 EMIHUDRAFT_451362 EMIHUDRAFT_452664 EMIHUDRAFT_460566(GLT1)
GTTGUITHDRAFT_91106
NGRNAEGRDRAFT_28878 NAEGRDRAFT_50501
 » show all
Reference
1  [PMID:6445358]
  Authors
Harpaz N, Schachter H.
  Title
Control of glycoprotein synthesis. Bovine colostrum UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase I. Separation from UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase II, partial purification, and substrate specificity.
  Journal
J Biol Chem 255:4885-93 (1980)
Reference
2  [PMID:6452163]
  Authors
Mendicino J, Chandrasekaran EV, Anumula KR, Davila M.
  Title
Isolation and properties of alpha-D-mannose:beta-1,2-N-acetylglucosaminyltransferase from trachea mucosa.
  Journal
Biochemistry 20:967-76 (1981)
DOI:10.1021/bi00507a050
Reference
3  [PMID:6450208]
  Authors
Oppenheimer CL, Hill RL.
  Title
Purification and characterization of a rabbit liver alpha 1 goes to 3 mannoside beta 1 goes to 2 N-acetylglucosaminyltransferase.
  Journal
J Biol Chem 256:799-804 (1981)
Reference
4  [PMID:6457827]
  Authors
Oppenheimer CL, Eckhardt AE, Hill RL.
  Title
The nonidentity of porcine N-acetylglucosaminyltransferases I and II.
  Journal
J Biol Chem 256:11477-82 (1981)
Reference
5  [PMID:6170335]
  Authors
Miyagi T, Tsuiki S.
  Title
Studies on UDP-N-acetylglucosamine : alpha-mannoside beta-N-acetylglucosaminyltransferase of rat liver and hepatomas.
  Journal
Biochim Biophys Acta 661:148-57 (1981)
DOI:10.1016/0005-2744(81)90094-2
Reference
6  [PMID:6366476]
  Authors
Schachter H, Narasimhan S, Gleeson P, Vella G.
  Title
Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type.
  Journal
Methods Enzymol 98:98-134 (1983)
DOI:10.1016/0076-6879(83)98143-0
Reference
7  [PMID:6235906]
  Authors
Vella GJ, Paulsen H, Schachter H.
  Title
Control of glycoprotein synthesis. IX. A terminal Man alpha l-3Man beta 1- sequence in the substrate is the minimum requirement for UDP-N-acetyl-D-glucosamine: alpha-D-mannoside (GlcNAc to Man alpha 1-3) beta 2-N-acetylglucosaminyltransferase I.
  Journal
Can J Biochem Cell Biol 62:409-17 (1984)
DOI:10.1139/o84-056
Reference
8  [PMID:11032794]
  Authors
Unligil UM, Zhou S, Yuwaraj S, Sarkar M, Schachter H, Rini JM
  Title
X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: catalytic mechanism and a new protein superfamily.
  Journal
EMBO J 19:5269-80 (2000)
DOI:10.1093/emboj/19.20.5269
  Sequence
[ocu:100009521]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.101
IUBMB Enzyme Nomenclature: 2.4.1.101
ExPASy - ENZYME nomenclature database: 2.4.1.101
BRENDA, the Enzyme Database: 2.4.1.101
CAS: 102576-81-8

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