KEGG   ENZYME: 2.4.1.125
Entry
EC 2.4.1.125                Enzyme                                 

Name
sucrose---1,6-alpha-glucan 3(6)-alpha-glucosyltransferase;
water-soluble-glucan synthase (misleading);
GTF-I;
GTF-S;
GTF-SI;
sucrose-1,6-alpha-glucan 3(6)-alpha-glucosyltransferase;
sucrose:1,6-alpha-D-glucan 3-alpha- and 6-alpha-glucosyltransferase;
sucrose:1,6-, 1,3-alpha-D-glucan 3-alpha- and 6-alpha-D-glucosyltransferase;
sucrose:1,6-alpha-D-glucan 3(6)-alpha-D-glucosyltransferase;
gtfB (gene name);
gtfC (gene name);
gtfD (gene name)
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
sucrose:(1->6)-alpha-D-glucan 3(6)-alpha-D-glucosyltransferase
Reaction(IUBMB)
(1) sucrose + [(1->6)-alpha-D-glucosyl]n = D-fructose + [(1->6)-alpha-D-glucosyl]n+1  [RN:R02120 R06066];
(2) sucrose + [(1->6)-alpha-D-glucosyl]n = D-fructose + (1->3)-alpha-D-glucosyl-[(1->6)-alpha-D-glucosyl]n
Reaction(KEGG)
R02120 R06066(G)
Substrate
sucrose [CPD:C00089];
[(1->6)-alpha-D-glucosyl]n
Product
D-fructose [CPD:C00095];
[(1->6)-alpha-D-glucosyl]n+1;
(1->3)-alpha-D-glucosyl-[(1->6)-alpha-D-glucosyl]n
Comment
The glucansucrases transfer a D-glucosyl residue from sucrose to a glucan chain. They are classified based on the linkage by which they attach the transferred residue. In some cases, in which the enzyme forms more than one linkage type, classification relies on the relative proportion of the linkages that are generated. This enzyme extends (1->6)-alpha-D-glucans by both alpha(1->3) and alpha(1->6) linkages, with one of the linkage types being dominant. cf. EC 2.4.1.140, alternansucrase.
History
EC 2.4.1.125 created 1984
Reference
1  [PMID:6216919]
  Authors
Mukasa H, Shimamura A, Tsumori H.
  Title
Purification and characterization of basic glucosyltransferase from Streptococcus mutans serotype c.
  Journal
Biochim Biophys Acta 719:81-9 (1982)
DOI:10.1016/0304-4165(82)90310-5
Reference
2  [PMID:6461359]
  Authors
Shimamura A, Tsumori H, Mukasa H.
  Title
Purification and properties of Streptococcus mutans extracellular glucosyltransferase.
  Journal
Biochim Biophys Acta 702:72-80 (1982)
DOI:10.1016/0167-4838(82)90028-0
Reference
3  [PMID:2937877]
  Authors
Tsumori H, Shimamura A, Mukasa H.
  Title
Purification and properties of extracellular glucosyltransferase synthesizing 1,6-, 1,3-alpha-D-glucan from Streptococcus mutans serotype a.
  Journal
J Gen Microbiol 131:3347-53 (1985)
DOI:10.1099/00221287-131-12-3347
Reference
4  [PMID:8737492]
  Authors
Fujiwara T, Tamesada M, Bian Z, Kawabata S, Kimura S, Hamada S
  Title
Deletion and reintroduction of glucosyltransferase genes of Streptococcus mutans  and role of their gene products in sucrose dependent cellular adherence.
  Journal
Microb Pathog 20:225-33 (1996)
DOI:10.1006/mpat.1996.0021
Reference
5  [PMID:10234842]
  Authors
Monchois V, Willemot RM, Monsan P
  Title
Glucansucrases: mechanism of action and structure-function relationships.
  Journal
FEMS Microbiol Rev 23:131-51 (1999)
DOI:10.1111/j.1574-6976.1999.tb00394.x
Reference
6  [PMID:21354427]
  Authors
Ito K, Ito S, Shimamura T, Weyand S, Kawarasaki Y, Misaka T, Abe K, Kobayashi T, Cameron AD, Iwata S
  Title
Crystal structure of glucansucrase from the dental caries pathogen Streptococcus  mutans.
  Journal
J Mol Biol 408:177-86 (2011)
DOI:10.1016/j.jmb.2011.02.028
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.125
IUBMB Enzyme Nomenclature: 2.4.1.125
ExPASy - ENZYME nomenclature database: 2.4.1.125
BRENDA, the Enzyme Database: 2.4.1.125
CAS: 81725-87-3

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