KEGG ENZYME: 2.4.1.14

ENZYME: 2.4.1.14

Entry

EC 2.4.1.14                 Enzyme

Name

sucrose-phosphate synthase;
UDP-glucose---fructose-phosphate glucosyltransferase;
sucrosephosphate---UDP glucosyltransferase;
UDP-glucose-fructose-phosphate glucosyltransferase;
SPS;
uridine diphosphoglucose-fructose phosphate glucosyltransferase;
sucrose 6-phosphate synthase;
sucrose phosphate synthetase;
sucrose phosphate-uridine diphosphate glucosyltransferase;
sucrose phosphate synthase;
UDP-glucose:D-fructose-6-phosphate 2-alpha-D-glucosyltransferase

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-alpha-D-glucose:D-fructose-6-phosphate 2-alpha-D-glucosyltransferase (configuration-retaining)

Reaction(IUBMB)

UDP-alpha-D-glucose + D-fructose 6-phosphate = UDP + sucrose 6F-phosphate [RN:R00766 R06073]

Reaction(KEGG)

R00766 R06073(G)

Substrate

UDP-alpha-D-glucose [CPD:C00029];
D-fructose 6-phosphate [CPD:C00085]

Product

UDP [CPD:C00015];
sucrose 6F-phosphate [CPD:C02591]

Comment

Requires Mg2+ or Mn2+ for maximal activity [2]. The enzyme from Synechocystis sp. strain PCC 6803 is not specific for UDP-glucose as it can use ADP-glucose and, to a lesser extent, GDP-glucose as substrates [2]. The enzyme from rice leaves is activated by glucose 6-phosphate but that from cyanobacterial species is not [2]. While the reaction catalysed by this enzyme is reversible, the enzyme usually works in concert with EC 3.1.3.24, sucrose-phosphate phosphatase, to form sucrose, making the above reaction essentially irreversible [3]. The F in sucrose 6F-phosphate is used to indicate that the fructose residue of sucrose carries the substituent.

History

EC 2.4.1.14 created 1961, modified 2008

Pathway

ec00500

Starch and sucrose metabolism

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K00696

sucrose-phosphate synthase

Genes

ATH:	AT1G04920(SPS3F) AT4G10120(ATSPS4F) AT5G11110(SPS2F) AT5G20280(SPS1F)

ALY:	9307526 9310055 9310678 9328314

CRB:	111829932 17881554 17884088 17900430

CSAT:

104705376 104706379 104707307 104721265 104736104 104737013 104737937 104739022 104754635 104762737 104769326 104770411

EUS:	EUTSA_v10006639mg EUTSA_v10012540mg EUTSA_v10012643mg EUTSA_v10028384mg

BRP:	103844199 103845691 103851339 103853568 103855902 103874391

BNA:	106348525 106366768 106370972 106371763 106377423 106397279 106399433 106407297 106410155 106425833 106430056 106439492 111210345

BOE:	106307062 106318106 106327013 106327645 106328688 106343737

RSZ:	108817601 108832089 108842455 108842585 108842662 108843725 108847230 108847612 108850228

THJ:	104799636 104801022 104824613

CPAP:

110806438 110808418 110811933 110815619

CIT:	102614911 102618138 102624502 102630462

CIC:	CICLE_v10004221mg CICLE_v10007311mg CICLE_v10007312mg CICLE_v100186551m

PVY:	116106627 116107458 116107849 116108366 116113477 116128988 116138026

MINC:

123194371 123201077 123205772 123211161 123229541

TCC:	18587764 18588834 18592795 18602682

GRA:	105769920 105773682 105777501 105782791 105793014

GHI:	107897149 107914058 107914804 107940066 107948694 107959558 107962903 121212592 121218323 121225232

GAB:	108464000 108465223 108467712 108477118 108484177

DZI:	111276696 111279832 111284186 111286073 111299386 111303296 111307848

EGR:	104437014 104445410 104449786 104456017

GMX:	100127429 100786531 100798787 100806543 100807527 100813168

GSJ:	114377082 114383700 114393926 114395909 114417471 114423351

PVU:	PHAVU_003G170100g PHAVU_005G002600g PHAVU_006G031700g

VRA:	106762335 106768365 106779669

VAR:	108334080 108343111 108344383

VUN:	114179600 114189190 114195859

CCAJ:

109805846 109811244 109816247 109816562

APRC:

113846990 113848265 113855239

MTR:	MTR_3g047380 MTR_4g115620 MTR_5g091340

CAM:	101493995 101499894 101506259

LJA:	Lj0g3v0058449.1(Lj0g3v0058449.1) Lj2g3v3101760.1(Lj2g3v3101760.1) Lj3g3v1955590.1(Lj3g3v1955590.1) Lj4g3v1893200.1(Lj4g3v1893200.1)

ADU:	107461295 107482790

AIP:	107638448 107640447

AHF:	112701590 112723321 112741002 112795700

LANG:

109341631 109345880 109354648

FVE:	101292871 101298507 101307526 101310276

RCN:	112169498 112169745 112185776 112196398

PPER:

18768098 18771657 18789554 18790710

PMUM:

103319718 103322685 103336553 103341128

PAVI:

110746034 110752667 110755941 110763790

PDUL:

117614681 117615054 117633910 117637877

MDM:	103400066 103411875 103443444 103444513 103447799 103456219(SPS)

PXB:	103932736 103932737 103937917 103946723 103949182 103952486 103953884 103956574

ZJU:	107410852 107422272 107422414 107427961

MNT:	21388866 21391872 21395843

CSV:	101208942 101220898 101221022

CMO:	103485403 103487408 103496894

BHJ:	120070979 120075610 120092858

MCHA:

111005729 111006848 111009771 111020244

CMAX:

111476601 111485105 111489806 111494894 111496997 111499039

CMOS:

111437230 111443549 111445569 111459930 111461522 111464322

CPEP:

111778331 111779763 111792846 111797285 111807059 111808523

RCU:	8259425 8272223 8280623 8281336

JCU:	105633156 105637611 105640094 105641278

HBR:	110636212 110646478 110650924 110672696

MESC:

110602239 110602984 110604864 110606036 110609238 110630655

POP:	18099919 18107084 18111090 7458381 7461652 7477753

PEU:	105115760 105121058 105122656 105134043 105137770 105140471

PALZ:

118032603 118034487 118048694 118051254 118052067 118055228

JRE:	108986051 108995651 109001391 109002797 109003426

QSU:	111984301 112016314 112017458

QLO:	115959955 115961052 115974921 115977118

TWL:	119997875 120002169 120005548 120005626 120011885

VVI:	100232974 100241955 100244135 100262385

VRI:	117905325 117907982 117912407 117913756 117914242 117924951

SLY:	101055573 101055611 101256154 543516(sps)

SPEN:

107004626 107025839 107028888 107031272

SOT:	102577833(SPS) 102581151 102604041 102604080

CANN:

107864313 107877734

NTA:	107761563 107766133(SPSA) 107780355 107785687 107789345 107802224 107805820 107826329(SPSC)

NSY:	104224363 104227396 104234884 104247669

NTO:	104095849 104101876 104106492 104110702

NAU:	109216583 109234218 109241833 109243985

INI:	109148848 109176232 109178772

ITR:	116002183 116011867 116016475

SIND:

105162148 105166439 105172946 105174736 105179612

OEU:	111373117 111392573 111399373 111403073 111408561

EGT:	105949818 105963576 105964664 105974295

SSPL:

121748656 121751659 121781701 121796179 121799662 121799921 121804469 121806350 121807650 121810008

HAN:	110893891 110898571 110912341 110928688 110941362

ECAD:

122579489 122584911 122595990 122598998 122604031

LSV:	111877807 111881846 111893900 111895225 111908812

CCAV:

112501211 112514403 112516508 112518594 112519393 112521073

DCR:	108193934 108214238 108218718 108220526 108226543

CSIN:

114289014 114291008 114322208

BVG:	104901950(SBSPS1) 104909043

SOE:	110777402 110801165

CQI:	110705371 110708009 110725575 110733612

NNU:	104586143 104590896 104592518 104593975 104602206

MING:

122066018 122066728 122069816 122071855 122088483

TSS:	122651663 122655556 122667329 122667610 122669730

PSOM:

113271575 113281149 113282757 113282998 113296752 113303738 113355868

NCOL:

116247930 116252430 116255426

OSA:	4324364 4328527 4341642 4345220 4350151

DOSA:

Os01t0919400-00(Os01g0919400) Os02t0184400-01(Os02g0184400) Os06t0643800-01(Os06g0643800) Os08t0301500-01(Os08g0301500) Os11t0236100-01(Os11g0236100)

OBR:	102699820 102704296 102710502 102716755 102718101

BDI:	100825323 100828234 100831622 100843974 100844859

ATS:	109738481 109762784 109780026 109780714 109787086

TDC:	119266644 119270082 119274305 119278137 119286552 119295218 119316235 119322957 119330970 119339893

TAES:

123063086 123071986 123076775 123086738 123091244 123136523 123144023 123149544 123171116 123172359 542867 542872 542873 542958 543405

SBI:	8067709 8071544 8074599 8079417 8079789

ZMA:	100275248 100384028 100384650 100501516 103626734 103650849 542711

SITA:

101752535 101759319 101760433 101761271 101781780

SVS:	117834187 117837615 117852965 117857684 117860446

PVIR:

120644018 120654875 120671280 120672924 120676459 120680159 120685802 120700739 120704034 120709064 120712039

PHAI:

112873537 112889701 112893063 112896580 112902630

PDA:	103704794 103706486 103710588 103710846 103711479

EGU:	105039053 105049657 105050124 105051141

MUS:	103980733 103981780(SPS) 103988055 103998686

DCT:	110094355 110103949 110108196

PEQ:	110024800 110031244

AOF:	109824717 109830237 109837222 109841155

ATR:	18427768 18442593 18447493

SMO:	SELMODRAFT_148419(Sps2) SELMODRAFT_81492(Sps1) SELMODRAFT_91988(Sps3)

PPP:	112286574 112292429

VCN:	VOLCADRAFT_57787

CSL:	COCSUDRAFT_48006

CVR:	CHLNCDRAFT_36181

APRO:

F751_1987

GSL:	Gasu_03810

SDK:	KHX94_14080

SYK:	KDN34_13255

SAVI:

JYB87_15195

MARQ:

MARGE09_P3578

MMT:	Metme_0591

MDN:	JT25_011210

METL:

U737_05520

MAH:	MEALZ_0614(sps)

MBUR:

EQU24_03045

MESL:

KKZ03_07365

TCX:

Tcr_1794

HTR:	EPV75_09710

HMAR:

HVMH_0656(sps)

THIO:

AYJ59_03310 AYJ59_04280

MEJ:

Q7A_1564

MEC:

Q7C_247

THIG:

FE785_04520

TXA:	HQN79_07950

TZO:	THMIRHAT_22260(sps)

THIS:

HZT40_13735

TLO:	J9253_00770

TANI:

J8380_02210

NOC:

Noc_3069

NHL:

Nhal_3941

NWA:

Nwat_3124

NWR:	E3U44_04210

ALV:	Alvin_0252

TVI:	Thivi_1158

TMB:	Thimo_1348

MPUR:

MARPU_12205

TEE:

Tel_08340

THIP:

N838_02220

THIM:

KFB96_10540

TBOG:

LT988_06535

HHC:	M911_07515

EBS:	ECTOBSL9_1499

TGR:

Tgr7_0708

TKM:

TK90_2312

TNI:	TVNIR_0279(mfpsA_[H])

TTI:	THITH_16115

TVR:

TVD_01115

APRS:

BI364_15095

HNA:	Hneap_0227

HAZ:	A9404_10345

WMA:	WM2015_745

GAI:	IMCC3135_06955(mfpsA_2)

GHL:	GM160_01005

TTC:	FOKN1_0257

HOL:	HORIV_03730

PAUR:

FGL86_10595

RAC:	RA876_16685

NEU:	NE1213(sps)

NET:

Neut_1080

NIT:	NAL212_0460

NII:	Nit79A3_2496

NMU:	Nmul_A2267

NLC:	EBAPG3_014535

MFA:

Mfla_2610

MEI:	Msip34_2763

MEP:

MPQ_2706

MEU:	ACJ67_09825

MPAU:

ZMTM_14650(sps)

SPLB:

SFPGR_05670(sps)

SNIV:

SFSGTM_16970(sps)

DEU:	DBW_2297(mfpsA)

DBA:

Dbac_2912

DSF:

UWK_00548

DAK:	DaAHT2_1337

DOL:

Dole_0650

DML:	Dmul_00730

DAT:	HRM2_24290(spsA)

DOV:	DSCO28_19360(sps)

DWD:	DSCW_13100(sps)

DALK:

DSCA_04260(sps)

DLI:

dnl_43730

MEA:	Mex_1p0091

MDI:

METDI0081

MEX:

Mext_0200

MCH:

Mchl_0143

MPO:

Mpop_0284

MZA:	B2G69_08380

MOR:

MOC_0555

META:

Y590_00410

MAQU:

Maq22A_1p31500(rfaG)

MPHY:

MCBMB27_05495

MEE:	DA075_19325

METD:

C0214_00935

METX:

A3862_28905

MMES:

MMSR116_31330

MIND:

mvi_56290(sps)

AUA:	M673_16940

AALA:

IGS74_12200

AALM:

LUX29_14090

SUAM:

BOO69_20920

SULD:

B5M07_05835

SPOT:

G6548_17000

SINL:

DSM14862_02294(ss2)

MARU:

FIU81_08605(mfpsA1)

PYE:	A6J80_01145

PARR:

EOJ32_16725

PLIA:

E4191_09880

PMAU:

CP157_03500(ss2)

MON:	G8E03_17040

FAP:	GR316_12205

SNJ:	A7E77_00920

SMY:	BJP26_09930

SPLK:

AV944_13210

SPAU:

DRN02_006105

SARI:

H5J25_14425

ANH:	A6F65_02402(mfpsA)

CMAN:

A9D14_15710

EGN:	BMF35_b0162

EFV:	CHH26_13865

PNS:	A9D12_00015

ABG:	Asbog_02203

LCK:	HN018_05115

MGM:

Mmc1_3516

AFR:

AFE_1551

AFE:	Lferr_1267

ACU:

Atc_1728

ACZ:	Acaty_c1478

AFI:	Acife_1677

AFJ:	AFERRID_23690

ATX:	GCD22_01375(sps1)

AFP:	K1Y48_11380

MAES:

Ga0123461_1718

HOR:	Hore_18210

HAS:	Halsa_0772

HPK:	Hprae_0492

HHL:	Halha_1826

AFT:	BBF96_05250

MBJ:	KQ51_01499(mfpsA)

RTX:	TI83_07365

RTC:	APU90_09710

RUB:	GBA63_05610

SYN:	sll0045(sps)

SYZ:	MYO_128450(sps)

SYY:	SYNGTS_2819(sps)

SYT:	SYNGTI_2818(sps)

SYS:	SYNPCCN_2817(sps)

SYQ:	SYNPCCP_2817(sps)

SYJ:	D082_07330(spsA)

SYO:	C7I86_14725

SYC:	syc0730_d(sps)

SYU:	M744_12710

SYF:	Synpcc7942_0808

SYD:	Syncc9605_2689

SYE:	Syncc9902_2315

SYG:

sync_2936

SYR:	SynRCC307_2529(sps)

SYX:	SynWH7803_2527

SYP:	SYNPCC7002_A0888(spsA)

SYNE:

Syn6312_2695

SYNK:

KR100_00785

SYNR:

KR49_08880

SYND:

KR52_02500

SYH:	Syncc8109_2757(spsA)

SYNW:

SynWH8103_02908(sps_sds)

SYV:	AWQ23_04600

SYL:	AWQ21_05185

SYNC:

CB0101_08175

SYW:	SYNW2520(sps)

CGC:	Cyagr_0980

CYI:	CBM981_2458

PMA:	Pro_1880(rfaG)

PMM:	PMM1711(sps)

PMT:

PMT_2268

PMN:	PMN2A_1322

PMI:	PMT9312_1803

PMB:	A9601_19201

PMC:	P9515_19021

PMF:	P9303_30171(sps)

PMG:	P9301_19011

PMH:	P9215_19831

PMJ:	P9211_18491

PME:	NATL1_21951(sps)

PRC:

EW14_2080

PRM:

EW15_2282

TVN:	NIES2134_108440(sps)

SLW:	BRW62_04370

TEL:	tlr0582(sps)

THN:	NK55_11890(sps)

THEC:

FFX45_12875

LET:	O77CONTIG1_00929(mfpsA_1)

THEU:

HPC62_22465

GEN:	GM3709_1827

GEE:	GM3708_1775

OXY:	HCG48_12990

MIC:	Mic7113_0591

OAC:	Oscil6304_3321

MPRO:

BJP34_19305

NPU:	Npun_F4709

NOS:	Nos7107_1808

NOP:	Nos7524_3554

NFL:	COO91_00241

NOE:	CLI64_14590

NSH:

GXM_05878

ANB:	ANA_C12881

ACY:	Anacy_5147

AWA:	AA650_11585

CSG:	Cylst_1866

CALT:

Cal6303_0199

NSP:	BMF81_01068

DOU:	BMF77_04107(mfpsA_3)

DFS:	HGD76_18580

AFLO:

HEQ12_02350

TOQ:	HCG51_31840

SOA:	G3M56_005245

RBA:	RB5197(sps)

PIR:	VN12_23145(mfpsA_3)

RUL:	UC8_59090(mfpsA_2)

RML:	FF011L_55410(mfpsA)

MFF:	MFFC18_51400(mfpsA)

ROL:	CA51_34360(mfpsA)

AHEL:

Q31a_17940(mfpsA_1)

BVO:	Pan97_31820(mfpsA_2)

RLC:	K227x_26780(mfpsA)

AMUC:

Pan181_07020(mfpsA_2)

PND:	Pla175_04860(mfpsA_1)

PLM:

Plim_3683

PBS:	Plabr_0007 Plabr_3287

FMR:	Fuma_03286(mfpsA_2)

GMR:	GmarT_26600(mfpsA)

GIM:	F1728_01675

GPN:	Pan110_25120(mfpsA)

MRI:	Mal4_00740(mfpsA_2)

SDYN:

Mal52_36180(mfpsA)

PLON:

Pla110_00640(mfpsA)

VBL:	L21SP4_02013

TAER:

GT409_01100

OCK:	EXM22_16825

NSO:	NIASO_06600

NIA:	A8C56_09200

LBY:

Lbys_0264

RSI:	Runsl_4419

FJO:

Fjoh_3967

FJG:	BB050_03561(mfpsA)

FCR:	HYN56_19040

FFL:	HYN86_15915

FEN:	J0383_21685

MRO:

MROS_1315

TME:

Tmel_0533

TAF:

THA_1341

THP:	BG95_06130

THER:

Y592_06205

TFE:	JYK00_00680

FNO:

Fnod_0446

FPE:	Ferpe_0508

FIA:	NA23_02855

OCY:	OSSY52_08760

PMO:

Pmob_1867

DTN:

DTL3_0896

KOL:

Kole_1069

KPF:	IX53_06510

MPG:	Theba_2030

MINF:

MESINF_0949

ASAC:

ATHSA_1493

DIN:	Selin_2400

DAP:	Dacet_2943

NLI:	G3M70_12405

» show all

Reference

1 [PMID:13769376]

Authors

MENDICINO J.

Title

Sucrose phosphate synthesis in wheat germ and green leaves.

Journal

J Biol Chem 235:3347-52 (1960)

Reference

2 [PMID:9852031]

Authors

Curatti L, Folco E, Desplats P, Abratti G, Limones V, Herrera-Estrella L, Salerno G

Title

Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803: identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli.

Journal

J Bacteriol 180:6776-9 (1998)
DOI:10.1128/JB.180.24.6776-6779.1998

Sequence

[syn:sll0045]

Reference

3 [PMID:15012296]

Authors

Huber SC, Huber JL

Title

ROLE AND REGULATION OF SUCROSE-PHOSPHATE SYNTHASE IN HIGHER PLANTS.

Journal

Annu Rev Plant Physiol Plant Mol Biol 47:431-444 (1996)
DOI:10.1146/annurev.arplant.47.1.431

Reference

4 [PMID:12062401]

Authors

Cumino A, Curatti L, Giarrocco L, Salerno GL

Title

Sucrose metabolism: Anabaena sucrose-phosphate synthase and sucrose-phosphate phosphatase define minimal functional domains shuffled during evolution.

Journal

FEBS Lett 517:19-23 (2002)
DOI:10.1016/S0014-5793(02)02516-4

Reference

5 [PMID:18424616]

Authors

Chua TK, Bujnicki JM, Tan TC, Huynh F, Patel BK, Sivaraman J

Title

The structure of sucrose phosphate synthase from Halothermothrix orenii reveals its mechanism of action and binding mode.

Journal

Plant Cell 20:1059-72 (2008)
DOI:10.1105/tpc.107.051193

Sequence

[hor:Hore_18210]