Entry
Name
alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase;
MGAT2 (gene name);
N-acetylglucosaminyltransferase II;
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II;
acetylglucosaminyltransferase II;
uridine diphosphoacetylglucosamine-mannoside alpha1->6-acetylglucosaminyltransferase;
uridine diphosphoacetylglucosamine-alpha-1,6-mannosylglycoprotein beta-1-2-N-acetylglucosaminyltransferase;
uridine diphosphoacetylglucosamine-alpha-D-mannoside beta1-2-acetylglucosaminyltransferase;
UDP-GlcNAc:mannoside alpha1-6 acetylglucosaminyltransferase;
alpha-1,6-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase;
GnTII;
GlcNAc-T II;
UDP-N-acetyl-D-glucosamine:6-(alpha-D-mannosyl)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-glucosamine:alpha-D-mannosyl-(1->6)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] [RN:
R05985 ]
Reaction(KEGG)
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:
C00043 ];
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
Product
UDP [CPD:
C00015 ];
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
Comment
The enzyme, found in plants and animals, participates in the processing of N-glycans in the Golgi apparatus. Its activity initiates the synthesis of the second antenna of di-antennary complex N-glycans. While the natural substrate (produced by EC
3.2.1.114 , mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase) is described here, the minimal substrate recognized by the enzyme is alpha-D-Man-(1->6)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)]-beta-D-Man-R.
History
EC 2.4.1.143 created 1984, modified 2001 (EC 2.4.1.51 created 1972, part incorporated 1984), modified 2018
Pathway
ec00513 Various types of N-glycan biosynthesis
Orthology
K00736 alpha-1,6-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Genes
CANG : 105504530 105515273(MGAT2)
CCAD : 122443453(MGAT2) 122447863 122447864 122447865 122447866 122447867
XLA : 414590(mgat2.L) 444674(mgat2.S) 446383(XB22169453.S) 734412(XB22169453.L)
XTR : 100493854 448439(mgat2)
NPR : 108786864(MGAT2) 108800163
RTEM : 120921219(MGAT2) 120935474
BBUF : 120981960(MGAT2) 120982837
BGAR : 122921666(MGAT2) 122923967
DRE : 100000478(mgat2) 100536673
SRX : 107726709 107728126 107730605 107732491
SANH : 107669415 107704049 107704350(mgat2) 107704380
SGH : 107557011(mgat2) 107559114 107564584 107568220
CCAR : 109077373 109082551
CAUA : 113068520 113068535 113081518 113094131 113120583
CGIB : 127938115 127938718 127984006 127984062
PTET : 122325099 122325306 122325553(mgat2)
LROH : 127151843(mgat2) 127183324
OMC : 131527027(si:ch73-91k6.2) 131527564(mgat2)
PPRM : 120491954 120491997(mgat2)
RKG : 130082342(mgat2) 130096157
MAMB : 125278544 125278617(mgat2)
CIDE : 127501944 127502121
CERY : 137018655(mgat2) 137019231(si:ch73-91k6.2)
MASI : 127409674 127409686 127416323 127416329
TROS : 130565683(mgat2) 130565734
TDW : 130434794(mgat2) 130434878
MANU : 129429379 129429732(mgat2)
CMAO : 118815594(mgat2) 118815848
EEE : 113569808(mgat2) 113571121
CHAR : 105899426 105909584(mgat2)
TRU : 101063078(mgat2) 101067570 115248114
TFS : 130515952 130516551(mgat2)
LCO : 104924696(mgat2) 104939903
NCC : 104943934 104961827(mgat2)
TBEN : 117498421 117498480(mgat2)
CGOB : 115003723(mgat2) 115028857
PGEO : 117439929(mgat2) 117439992
GACU : 117554261(mgat2) 117554352
EMAC : 134876850(si:ch73-91k6.2) 134877034(mgat2)
ELY : 117270410 117270422(mgat2)
EFO : 125897284(mgat2) 125897386
PLEP : 121955204 121955423(mgat2)
SLUC : 116047014 116066305(mgat2)
ECRA : 117961267 117961951(mgat2)
ESP : 116706283 116706459(mgat2)
PFLV : 114573029 114573244(mgat2)
GAT : 120807798 120808197(mgat2)
PPUG : 119194871 119195149(mgat2)
AFB : 129107102 129107291(mgat2)
CLUM : 117727466 117727859(mgat2)
PSWI : 130202495 130202638(mgat2)
MSAM : 119908417 119909037(mgat2)
SCHU : 122862722 122863645(mgat2)
CUD : 121520941(mgat2) 121520955
ALAT : 119012696(mgat2) 119012778
MZE : 101475602(mgat2) 101487794
ONL : 100702968(mgat2) 100711814
OAU : 116315448 116332277(mgat2)
OLA : 101158071 101165022(mgat2)
OML : 112158265(mgat2) 112158540
CSAI : 133464470(mgat2) 133464895(si:ch73-91k6.2)
XMA : 102219064(mgat2) 102232240
XCO : 114158801(mgat2) 114158863
XHE : 116733833 116734508(mgat2)
PRET : 103457757 103457913(mgat2)
PFOR : 103148804 103155536(mgat2)
PLAI : 106944988 106955546(mgat2)
PMEI : 106907813 106916351(mgat2)
GAF : 122825395 122825486(mgat2)
PPRL : 129369098(mgat2) 129369189
CVG : 107100256 107100291(mgat2)
CTUL : 119772555 119794785(mgat2)
GMU : 124881532 124881579(mgat2)
NFU : 107378078(mgat2) 107378240
KMR : 108231761(mgat2) 108245450
ALIM : 106518585(mgat2) 106526872
NWH : 119418026 119418101(mgat2)
AOCE : 111567873 111573805(mgat2)
MCEP : 124998853 124999150(mgat2)
CSEM : 103380991(mgat2) 103398574
POV : 109632274 109632561(mgat2)
SSEN : 122783640(mgat2) 122784509
HHIP : 117758352(mgat2) 117758443
HSP : 118099414 118099590(mgat2)
PPLT : 128460003 128460463(mgat2)
SMAU : 118289728 118290381(mgat2)
SDU : 111238109 111240326(mgat2)
SLAL : 111661587 111672727(mgat2)
XGL : 120788860 120789389(mgat2)
HCQ : 109531026(mgat2) 109531778
SSCV : 125989898 125990094
SBIA : 133496168(si:ch73-91k6.2) 133497035(mgat2)
PEE : 133416998(mgat2) 133417343(si:ch73-91k6.2)
PTAO : 133468032(mgat2) 133468548(si:ch73-91k6.2)
BPEC : 110159416 110168298(mgat2)
MALB : 109962268(mgat2) 109969812
BSPL : 114849593 114849823(mgat2)
SJO : 128377120(mgat2) 128377534
SASA : 100195434(mgat2) 106571466 106571583 106607880
STRU : 115169272 115174519 115174718 115191872
OTW : 112217651 112217850 112250375 112250545
OMY : 110522294 110522477 110529676 110529848
OGO : 123990818 123990971 123995150 123995706
ONE : 115107821 115107970 115119249 115139349
OKI : 109866558 109879217 109900801 109900946
OKE : 118378607 118386968 118398436 118398600
SALP : 111954414 111973071 112068315 112068750
SNH : 120019015 120019103 120024105 120024579
CCLU : 121548644 121548856 121552466 121552469
ELS : 105017600 105030576(mgat2)
SFM : 108921073 108941417(mgat2)
PKI : 111852433(mgat2) 111858011
AANG : 118210179 118229105 118231413
LOC : 102691853 102696707(mgat2)
PSPA : 121315985 121324269 121330054
ARUT : 117402350 117416573 117422019
LCM : 102345092 102367380(MGAT2)
CMK : 103175354(mgat2) 103177928
PMRN : 116939570(MGAT2) 116948409
SCLV : 120333638 120333644
DSI : Dsimw501_GD17443(Dsim_GD17443)
DMO : Dmoj_GI18065 Dmoj_GI21962
GFS : 119640797 119640839 119640958
AALB : 109421111 115255943
CQU : CpipJ_CPIJ003882 CpipJ_CPIJ003883 CpipJ_CPIJ003884
CNS : 116341843 116341969 116341970
DVV : 114343906 126887627 126887628
APLN : 108735397 108735401 108741847
MSEX : 115448762 115450705 119193149
DPL : KGM_201233 KGM_201630 KGM_204435 KGM_209356 KGM_212698
MCIX : 123657498 123665895
CCRC : 123695713 123704767
HZE : 124633158 124634639 124644581
SLIU : 111355981 111360251 111362581
OFU : 114356381 114356809 114360856
PXY : 105387461 105387933 105389083
PGW : 126369088 126369836 126379475
LGLY : 125224639 125239332 125241626
BTAB : 109034266 109035413
HHAL : 106677707 106687803
HVI : 124352699 124352700 124352702
TPAL : 117641824 117644342
BROR : 134542370 134542371
PJA : 122246174 122255494 122262869
PCHN : 125033476 125038720 125043563
PMOO : 119585763 119598120
HAME : 121871078 121873036 121878147
PCLA : 123759722 123768707 123770318
CQD : 128688961 128695676 128703376
PTRU : 123510656 123515468
MNZ : 135197071 135212291 135218983
HAZT : 108666182 108674922
RSAN : 119372332 119373655
RMP : 119164886 119166737 119182772
CSCU : 111615171 111633246
PMEO : 129583744 129596224
CEL : CELE_C03E10.4(gly-20)
CBR : CBG_23158(Cbr-gly-20)
BMY : BM_BM1934(Bma-gly-20)
LGI : LOTGIDRAFT_108438 LOTGIDRAFT_119931
PVUL : 126808913 126811976 126816648
PCAN : 112561356 112561357 112565215
GAE : 121367249 121383477 121384942 121387206 121387207 121387209 121387210 121387212 121387213 121387214 121387216 121387217 121387805
HRF : 124120692 124121627 124136605
HRJ : 124253015 124255726 124284964 124288454
DPOL : 127841252 127866703 127869196
SHX : MS3_00007943(MGAT2_1)
ATEN : 116297215 116299645
AMIL : 114956745 114958098
PDAM : 113669309 113683959
SPIS : 111323246 111331994
DGT : 114523668 114525536 114525537 114538190
XEN : 124435367 124451129 124451146 124451931
TAD : TRIADDRAFT_31240 TRIADDRAFT_52327 TRIADDRAFT_60274
CSAT : 104698925 104747180 104766544
GHI : 107912457 107922694 107954941
HSYR : 120126768 120166120
QSA : O6P43_014467 O6P43_020716
MSYL : 126583274 126610728
CPEP : 111786923 111811705
SIND : 105159785 105162532
SSPL : 121748633 121750698
APAN : 127264435 127266243
CSIN : 114289886 114313614
AEW : 130751168 130755260 130761594
NNU : 104600174 104600930 104602893
PSOM : 113336956 113348468
TAES : 123139160 123145934
PVIR : 120645127 120657494
ZOF : 122011760 122014723 122041444
SMO : SELMODRAFT_80130(GntII)
SRE : PTSG_04838 PTSG_08664 PTSG_09901
» show all
Taxonomy
Reference
Authors
Harpaz N, Schachter H.
Title
Control of glycoprotein synthesis. Bovine colostrum UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase I. Separation from UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase II, partial purification, and substrate specificity.
Journal
J Biol Chem 255:4885-93 (1980)
Reference
Authors
Mendicino J, Chandrasekaran EV, Anumula KR, Davila M.
Title
Isolation and properties of alpha-D-mannose:beta-1,2-N-acetylglucosaminyltransferase from trachea mucosa.
Journal
Reference
Authors
Oppenheimer CL, Eckhardt AE, Hill RL.
Title
The nonidentity of porcine N-acetylglucosaminyltransferases I and II.
Journal
J Biol Chem 256:11477-82 (1981)
Reference
Authors
Schachter H, Narasimhan S, Gleeson P, Vella G.
Title
Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type.
Journal
Reference
Authors
Bendiak B, Schachter H
Title
Control of glycoprotein synthesis. Kinetic mechanism, substrate specificity, and inhibition characteristics of UDP-N-acetylglucosamine:alpha-D-mannoside beta 1-2 N-acetylglucosaminyltransferase II from rat liver.
Journal
J Biol Chem 262:5784-90 (1987)
Sequence
Reference
Authors
Bendiak B, Schachter H.
Title
Control of glycoprotein synthesis. Purification of UDP-N-acetylglucosamine:alpha-D-mannoside beta 1-2 N-acetylglucosaminyltransferase II from rat liver.
Journal
J Biol Chem 262:5775-83 (1987)
Reference
Authors
Tan J, D'Agostaro AF, Bendiak B, Reck F, Sarkar M, Squire JA, Leong P, Schachter H
Title
The human UDP-N-acetylglucosamine: alpha-6-D-mannoside-beta-1,2- N-acetylglucosaminyltransferase II gene (MGAT2). Cloning of genomic DNA, localization to chromosome 14q21, expression in insect cells and purification of the recombinant protein.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 2.4.1.143