KEGG ENZYME: 2.4.1.146

ENZYME: 2.4.1.146

Entry

EC 2.4.1.146                Enzyme

Name

beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase;
O-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II;
uridine diphosphoacetylglucosamine-mucin beta(1->3)-acetylglucosaminyltransferase (elongating);
elongation 3beta-GalNAc-transferase;
UDP-N-acetyl-D-glucosamine:O-glycosyl-glycoprotein (N-acetyl-D-glucosamine to beta-D-galactose of beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R) beta-1,3-N-acetyl-D-glucosaminyltransferase;
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->3)-[N-acetyl-D-glucosaminyl-(1->6)]-N-acetyl-D-galactosaminyl-R 3-beta-N-acetyl-D-glucosaminyltransferase;
B3GNT3 (gene name)

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-N-acetyl-alpha-D-glucosamine:3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] 3-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)

Reaction(IUBMB)

UDP-N-acetyl-alpha-D-glucosamine + 3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] = UDP + 3-O-{N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] [RN:R04636 R05901]

Reaction(KEGG)

R04636 R05901(G)

Substrate

UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein]

Product

UDP [CPD:C00015];
3-O-{N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein]

Comment

The enzyme catalyses the addition of N-acetyl-alpha-D-glucosamine to the core 2 structure of O-glycans.

History

EC 2.4.1.146 created 1984, modified 2018

Orthology

K07970

beta-1,3-N-acetylglucosaminyltransferase 3

Genes

HSA:	10331(B3GNT3)

PTR:	455847(B3GNT3)

PPS:	100982986(B3GNT3)

GGO:	101151557(B3GNT3)

PON:	100433243(B3GNT3)

NLE:	100596245(B3GNT3)

MCC:	114674034(B3GNT3) 719221

MCF:	102134304(B3GNT3)

CSAB:

103234153(B3GNT3)

CATY:

105601677(B3GNT3)

PANU:

101005136(B3GNT3)

TGE:	112613343(B3GNT3)

RRO:	104660961(B3GNT3)

RBB:	108534279(B3GNT3)

TFN:	117076459(B3GNT3)

PTEH:

111554311(B3GNT3)

CJC:	100404385(B3GNT3)

SBQ:	101033879(B3GNT3)

MMUR:

105854991(B3GNT3)

OGA:	100961131(B3GNT3)

MMU:	72297(B3gnt3)

MCAL:

110299618(B3gnt3)

MPAH:

110337004(B3gnt3)

RNO:	290638(B3gnt3)

MCOC:

116071470(B3gnt3)

MUN:	110549208(B3gnt3)

CGE:	100773213(B3gnt3)

PLEU:

114687946(B3gnt3)

NGI:	103726889(B3gnt3)

HGL:	101713138(B3gnt3)

CPOC:

100713343(B3gnt3)

CCAN:

109698339(B3gnt3)

DSP:	122100853(B3gnt3)

NCAR:

124969063

OPI:	101527594(B3GNT3)

TUP:	102472378(B3GNT3)

CFA:	484831(B3GNT3)

VVP:	112913174(B3GNT3)

VLG:	121494311(B3GNT3)

AML:	100473756(B3GNT3)

UMR:	103675168(B3GNT3)

UAH:	113256690(B3GNT3)

UAR:	123780002(B3GNT3)

ELK:

111144118

LLV:

125082431

MPUF:

101688106(B3GNT3)

ORO:	101368545(B3GNT3)

EJU:	114226652(B3GNT3)

ZCA:	113917507(B3GNT3)

MLX:	118005244(B3GNT3)

FCA:	101092089(B3GNT3)

PYU:	121010990(B3GNT3)

PBG:	122487077(B3GNT3)

PTG:	102965987(B3GNT3)

PPAD:

109247961(B3GNT3)

AJU:	106969093(B3GNT3)

HHV:	120239150(B3GNT3)

BTA:	784997(B3GNT3)

BOM:	102281061(B3GNT3)

BIU:	109560822(B3GNT3)

BBUB:

102400108(B3GNT3)

CHX:	102171777(B3GNT3)

OAS:	101113066(B3GNT3)

ODA:	120864228(B3GNT3)

CCAD:

122440724(B3GNT3)

SSC:	100518473(B3GNT3)

CFR:	102512477(B3GNT3)

CBAI:

105082559(B3GNT3)

CDK:	105101856(B3GNT3)

VPC:	102538202(B3GNT3)

BACU:

103012961(B3GNT3)

LVE:	103080965(B3GNT3)

OOR:	101282171(B3GNT3)

DLE:	111166586(B3GNT3)

PCAD:

102989486(B3GNT3)

PSIU:

116750777(B3GNT3)

ECB:	100146466(B3GNT3)

EPZ:	103542315(B3GNT3)

EAI:	106830835(B3GNT3)

MYB:	102254606(B3GNT3)

MYD:	102767713(B3GNT3)

MMYO:

118658722(B3GNT3)

MLF:	102428358(B3GNT3)

MNA:	107535321(B3GNT3)

PKL:	118722609 118725871(B3GNT3)

HAI:	109382386(B3GNT3)

DRO:	112305269(B3GNT3)

SHON:

118975180 118975182

AJM:	119043950 119046818(B3GNT3) 119046819

PDIC:

114503731 114503735

PHAS:

123828315 123828458

MMF:	118616497(B3GNT3)

RFQ:	117038056(B3GNT3)

PALE:

102882932(B3GNT3)

PGIG:

120606538(B3GNT3)

PVP:	105296673(B3GNT3)

RAY:	107521817(B3GNT3)

MJV:	108388013(B3GNT3)

TOD:	119241108(B3GNT3)

SARA:

101553979(B3GNT3)

LAV:	100673587(B3GNT3)

MDO:	100010637(B3GNT3)

GAS:	123232263(B3GNT3)

SHR:	100920625(B3GNT3)

PCW:	110223495(B3GNT3)

OAA:	100086714(B3GNT3)

GGA:	112530425(B3GNT3)

PCOC:

116235881

MGP:	100549756(B3GNT3)

CJO:	107325454(B3GNT3)

NMEL:

110388735(B3GNT3)

ACYG:

106048207(B3GNT3)

FPG:	106112886(B3GNT3)

FCH:	102054592(B3GNT3)

CLV:	102095902(B3GNT3)

EGZ:	104124478(B3GNT3)

NNI:	104013696(B3GNT3)

TALA:

116963233 122153644

ACHC:

115348859(B3GNT3)

AAM:

106486607

AROW:

112965649(B3GNT3)

NPD:	112949098(B3GNT3)

DNE:	112992748(B3GNT3)

ASN:	102373956(B3GNT3)

AMJ:	106737906(B3GNT3)

CPOO:

109318684(B3GNT3)

GGN:	109302437(B3GNT3)

PSS:	102443521(B3GNT3)

CMY:	102944348(B3GNT3)

CPIC:

101936651(B3GNT3)

TST:	117869061(B3GNT3)

CABI:

116833222(B3GNT3)

MRV:	120391923(B3GNT3)

PVT:	110072506(B3GNT3)

SUND:

121936496(B3GNT3)

PBI:	103061136(B3GNT3)

PMUR:

107285005 107285006 107296837(B3GNT3) 114922368

TSR:

106556824

PGUT:

117670585(B3GNT3)

VKO:	123018694(B3GNT3)

PMUA:

114588656(B3GNT3)

ZVI:	118087801(B3GNT3)

GJA:	107120629(B3GNT3)

XLA:	108703715(b3gnt3.2.S) 108711807 734264(b3gnt3.2.L)

XTR:	100145701(b3gnt3.2) 100380117(b3gnt3.1) 100494334 101733889 116409851

NPR:	108790801 108793753 108803023

RTEM:

120909662 120909687 120914440 120933672 120933692

BBUF:

120989390(B3GNT3) 121009144

BGAR:

122919843 122920562(B3GNT3)

DRE:	100002020(b3gnt3.2) 561172(b3gnt3.4)

SRX:

107755313

SANH:

107665060 107700302(b3gnt3)

SGH:

107599054

CCAR:

109083431 109099585

CAUA:

113049247 113077616 113099571

IPU:	108265513 108265849 108266016 108266017

PHYP:

113525137(b3gnt3)

SMEO:

124399388 124400092 124400431

TFD:	113634256 113634260 113642616 113642617 113642618 113642619 113642620 125146324

AMEX:

103026573(b3gnt3)

EEE:	113587291 113587962

TRU:	101072648(b3gnt3)

TNG:	GSTEN00019098G001 GSTEN00019099G001 GSTEN00019100G001

LCO:	104935378 109139021(b3gnt3) 109139346 109139347

NCC:

104958016

CGOB:

115007161(b3gnt3)

ELY:	117253529 117263620 117264498

PLEP:

121941118 121952728

SLUC:

116055449

ECRA:

117950580

PFLV:

114561872(b3gnt3)

GAT:

120824339

PPUG:

119217180

MSAM:

119900537 119900538 119900539 119917039

CUD:	121512695 121519212 121519214 121519215

MZE:	101474209 105940915

ONL:	100700249 100710618 102077342(b3gnt3)

OAU:	116328445 116328458 116331915 116333906

OLA:	101163432 101173163 101173646 101175309

OML:	112138882 112139123 112142676 112142717 112150918 112150945

XMA:	102222047 102226121 102235218(b3gnt3) 111606492

XCO:	114137712 114137713 114150430 114151525 114152730

XHE:	116713044 116713045 116725213 116725996 116727933

PRET:

103463427 103463431 103475708 103476783

PFOR:

103129919 103129923 103133540 103133955

PLAI:

106937802 106937900 106947686 106955559

PMEI:

106906127 106914022 106914028 106914127

GAF:	122838849 122838850 122842547 122845157

CVG:	107095630 107103340(b3gnt3) 107103343 107103671 107104833

CTUL:

119773651 119786226 119786238 119787603 119787742 119790866

GMU:	124858982 124873366 124874498 124876850

NFU:	107376009 107386572 107392806

KMR:	108234252 108234256 108243045 108247418 119616708

ALIM:

106519220 106519221 106533410

NWH:	119407675 119407676 119420122 119430271

AOCE:

111576549(b3gnt3)

CSEM:

103395334 103395738 103395759 103399385

POV:	109636623(b3gnt3)

SSEN:

122780488 122781014 122781015

HHIP:

117760258 117760259 117760260 117760575 117760577

LCF:	108881191 108893170

SDU:	111218271(b3gnt3) 111235274

SLAL:

111669970(b3gnt3) 111670022

XGL:	120790634 120791330 120802899

HCQ:	109519123(b3gnt3)

BPEC:

110160181

MALB:

109958356

SASA:

106573871(b3gnt3) 106578060 106613654

OTW:	112250760(b3gnt3.4) 112252159

OMY:	110496754 110524171

OGO:	123996683 123998437 124007935

ONE:

115109173

SALP:

111953012(b3gnt3)

SNH:	120053736 120054432

ELS:	105007499 105011432(b3gnt3) 105031420

SFM:	108930515(b3gnt3)

PKI:	111860178(b3gnt3)

AANG:

118221742 118226430

LOC:	102687306(b3gnt3)

PSPA:

121301356 121301670

ARUT:

117401222 117966163 117966195 117966263 117966282 117966310 117968039 117972057

LCM:	102345207(B3GNT3)

CMK:

103181970

RTP:

109918138

MSEX:

115456046

PPOT:

106102791

» show all

Reference

1 [PMID:6226356]

Authors

Brockhausen I, Rachaman ES, Matta KL, Schachter H.

Title

The separation by liquid chromatography (under elevated pressure) of phenyl, benzyl, and O-nitrophenyl glycosides of oligosaccharides. Analysis of substrates and products for four N-acetyl-D-glucosaminyl-transferases involved in mucin synthesis.

Journal

Carbohydr Res 120:3-16 (1983)
DOI:10.1016/0008-6215(83)88002-1

Reference

2 [PMID:11042166]

Authors

Shiraishi N, Natsume A, Togayachi A, Endo T, Akashima T, Yamada Y, Imai N, Nakagawa S, Koizumi S, Sekine S, Narimatsu H, Sasaki K

Title

Identification and characterization of three novel beta 1,3-N-acetylglucosaminyltransferases structurally related to the beta 1,3-galactosyltransferase family.

Journal

J Biol Chem 276:3498-507 (2001)
DOI:10.1074/jbc.M004800200

Sequence

[hsa:10331]

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.146

IUBMB Enzyme Nomenclature:

2.4.1.146

ExPASy - ENZYME nomenclature database:

2.4.1.146

BRENDA, the Enzyme Database:

2.4.1.146

CAS:	87927-99-9

DBGET integrated database retrieval system