KEGG   ENZYME: 2.4.1.146
Entry
EC 2.4.1.146                Enzyme                                 

Name
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase;
O-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II;
uridine diphosphoacetylglucosamine-mucin beta(1->3)-acetylglucosaminyltransferase (elongating);
elongation 3beta-GalNAc-transferase;
UDP-N-acetyl-D-glucosamine:O-glycosyl-glycoprotein (N-acetyl-D-glucosamine to beta-D-galactose of beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R) beta-1,3-N-acetyl-D-glucosaminyltransferase;
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->3)-[N-acetyl-D-glucosaminyl-(1->6)]-N-acetyl-D-galactosaminyl-R 3-beta-N-acetyl-D-glucosaminyltransferase;
B3GNT3 (gene name)
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-N-acetyl-alpha-D-glucosamine:3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] 3-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + 3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] = UDP + 3-O-{N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] [RN:R04636 R05901]
Reaction(KEGG)
R04636 R05901(G)
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein]
Product
UDP [CPD:C00015];
3-O-{N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein]
Comment
The enzyme catalyses the addition of N-acetyl-alpha-D-glucosamine to the core 2 structure of O-glycans.
History
EC 2.4.1.146 created 1984, modified 2018
Orthology
K07970  beta-1,3-N-acetylglucosaminyltransferase 3
Genes
HSA: 10331(B3GNT3)
PTR: 455847(B3GNT3)
PPS: 100982986(B3GNT3)
GGO: 101151557(B3GNT3)
PON: 100433243(B3GNT3)
NLE: 100596245(B3GNT3)
MCC: 114674034(B3GNT3) 719221
MCF: 102134304(B3GNT3)
CSAB: 103234153(B3GNT3)
RRO: 104660961(B3GNT3)
RBB: 108534279(B3GNT3)
CJC: 100404385(B3GNT3)
SBQ: 101033879(B3GNT3)
MMU: 72297(B3gnt3)
MCAL: 110299618(B3gnt3)
MPAH: 110337004(B3gnt3)
RNO: 290638(B3gnt3)
MUN: 110549208(B3gnt3)
CGE: 100773213(B3gnt3)
NGI: 103726889(B3gnt3)
HGL: 101713138(B3gnt3)
CCAN: 109698339(B3gnt3)
TUP: 102472378(B3GNT3)
CFA: 484831(B3GNT3)
VVP: 112913174(B3GNT3)
AML: 100473756(B3GNT3)
UMR: 103675168(B3GNT3)
UAH: 113256690(B3GNT3)
ORO: 101368545(B3GNT3)
ELK: 111144118
FCA: 101092089(B3GNT3)
PTG: 102965987(B3GNT3)
PPAD: 109247961(B3GNT3)
AJU: 106969093(B3GNT3)
BTA: 784997(B3GNT3)
BOM: 102281061(B3GNT3)
BIU: 109560822(B3GNT3)
BBUB: 102400108(B3GNT3)
CHX: 102171777(B3GNT3)
OAS: 101113066(B3GNT3)
SSC: 100518473(B3GNT3)
CFR: 102512477(B3GNT3)
CDK: 105101856(B3GNT3)
BACU: 103012961(B3GNT3)
LVE: 103080965(B3GNT3)
OOR: 101282171(B3GNT3)
DLE: 111166586(B3GNT3)
PCAD: 102989486(B3GNT3)
ECB: 100146466(B3GNT3)
EPZ: 103542315(B3GNT3)
EAI: 106830835(B3GNT3)
MYB: 102254606(B3GNT3)
MYD: 102767713(B3GNT3)
MNA: 107535321(B3GNT3)
HAI: 109382386(B3GNT3)
DRO: 112305269(B3GNT3)
PALE: 102882932(B3GNT3)
RAY: 107521817(B3GNT3)
MJV: 108388013(B3GNT3)
LAV: 100673587(B3GNT3)
MDO: 100010637(B3GNT3)
SHR: 100920625(B3GNT3)
PCW: 110223495(B3GNT3)
OAA: 100086714(B3GNT3)
GGA: 112530425(B3GNT3)
MGP: 100549756(B3GNT3)
CJO: 107325454(B3GNT3)
NMEL: 110388735(B3GNT3)
ACYG: 106048207(B3GNT3)
FPG: 106112886(B3GNT3)
FCH: 102054592(B3GNT3)
CLV: 102095902(B3GNT3)
EGZ: 104124478(B3GNT3)
NNI: 104013696(B3GNT3)
AAM: 106486607
ASN: 102373956(B3GNT3)
AMJ: 106737906(B3GNT3)
PSS: 102443521(B3GNT3)
CMY: 102944348(B3GNT3)
CPIC: 101936651(B3GNT3)
PVT: 110072506(B3GNT3)
PBI: 103061136(B3GNT3)
TSR: 106556824
PMUA: 114588656(B3GNT3)
GJA: 107120629(B3GNT3)
XTR: 100145701(b3gnt3.2) 100380117(b3gnt3.1) 100494334 101733889 116409851
DRE: 100002020(b3gnt3.2) 561172(b3gnt3.4)
SRX: 107755313
SANH: 107665060 107700302(b3gnt3)
SGH: 107599054
PHYP: 113525137(b3gnt3)
AMEX: 103026573(b3gnt3)
TRU: 101072648(b3gnt3)
NCC: 104958016
AOCE: 111576549(b3gnt3)
POV: 109636623(b3gnt3)
SDU: 111218271(b3gnt3) 111235274
SLAL: 111669970(b3gnt3) 111670022
HCQ: 109519123(b3gnt3)
BPEC: 110160181
MALB: 109958356
OTW: 112250760
SALP: 111953012(b3gnt3)
SFM: 108930515(b3gnt3)
PKI: 111860178(b3gnt3)
LCM: 102345207(B3GNT3)
CMK: 103181970(b3gnt3)
RTP: 109918138
 » show all
Reference
1  [PMID:6226356]
  Authors
Brockhausen I, Rachaman ES, Matta KL, Schachter H.
  Title
The separation by liquid chromatography (under elevated pressure) of phenyl, benzyl, and O-nitrophenyl glycosides of oligosaccharides. Analysis of substrates and products for four N-acetyl-D-glucosaminyl-transferases involved in mucin synthesis.
  Journal
Carbohydr Res 120:3-16 (1983)
DOI:10.1016/0008-6215(83)88002-1
Reference
2  [PMID:11042166]
  Authors
Shiraishi N, Natsume A, Togayachi A, Endo T, Akashima T, Yamada Y, Imai N, Nakagawa S, Koizumi S, Sekine S, Narimatsu H, Sasaki K
  Title
Identification and characterization of three novel beta 1,3-N-acetylglucosaminyltransferases structurally related to the beta 1,3-galactosyltransferase family.
  Journal
J Biol Chem 276:3498-507 (2001)
DOI:10.1074/jbc.M004800200
  Sequence
[hsa:10331]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.146
IUBMB Enzyme Nomenclature: 2.4.1.146
ExPASy - ENZYME nomenclature database: 2.4.1.146
BRENDA, the Enzyme Database: 2.4.1.146
CAS: 87927-99-9

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