KEGG ENZYME: 2.4.1.146

ENZYME: 2.4.1.146

Entry

EC 2.4.1.146 Enzyme

Name

beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase;
O-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II;
uridine diphosphoacetylglucosamine-mucin beta(1->3)-acetylglucosaminyltransferase (elongating);
elongation 3beta-GalNAc-transferase;
UDP-N-acetyl-D-glucosamine:O-glycosyl-glycoprotein (N-acetyl-D-glucosamine to beta-D-galactose of beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R) beta-1,3-N-acetyl-D-glucosaminyltransferase;
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->3)-[N-acetyl-D-glucosaminyl-(1->6)]-N-acetyl-D-galactosaminyl-R 3-beta-N-acetyl-D-glucosaminyltransferase;
B3GNT3 (gene name)

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-N-acetyl-alpha-D-glucosamine:3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] 3-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)

Reaction(IUBMB)

UDP-N-acetyl-alpha-D-glucosamine + 3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] = UDP + 3-O-{N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] [RN:R04636 R05901]

Reaction(KEGG)

R04636 R05901(G)

Substrate

UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein]

Product

UDP [CPD:C00015];
3-O-{N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein]

Comment

The enzyme catalyses the addition of N-acetyl-alpha-D-glucosamine to the core 2 structure of O-glycans.

History

EC 2.4.1.146 created 1984, modified 2018

Orthology

K07970

beta-1,3-N-acetylglucosaminyltransferase 3

Genes

HSA:	10331(B3GNT3)

PTR:	455847(B3GNT3)

PPS:	100982986(B3GNT3)

GGO:	101151557(B3GNT3)

PON:	100433243(B3GNT3)

NLE:	100596245(B3GNT3)

MCC:	114674034(B3GNT3) 719221

MCF:	102134304(B3GNT3)

CSAB:

103234153(B3GNT3)

RRO:	104660961(B3GNT3)

RBB:	108534279(B3GNT3)

CJC:	100404385(B3GNT3)

SBQ:	101033879(B3GNT3)

MMU:	72297(B3gnt3)

MCAL:

110299618(B3gnt3)

MPAH:

110337004(B3gnt3)

RNO:	290638(B3gnt3)

MUN:	110549208(B3gnt3)

CGE:	100773213(B3gnt3)

NGI:	103726889(B3gnt3)

HGL:	101713138(B3gnt3)

CCAN:

109698339(B3gnt3)

TUP:	102472378(B3GNT3)

CFA:	484831(B3GNT3)

VVP:	112913174(B3GNT3)

AML:	100473756(B3GNT3)

UMR:	103675168(B3GNT3)

UAH:	113256690(B3GNT3)

ORO:	101368545(B3GNT3)

ELK:

111144118

FCA:	101092089(B3GNT3)

PTG:	102965987(B3GNT3)

PPAD:

109247961(B3GNT3)

AJU:	106969093(B3GNT3)

BTA:	784997(B3GNT3)

BOM:	102281061(B3GNT3)

BIU:	109560822(B3GNT3)

BBUB:

102400108(B3GNT3)

CHX:	102171777(B3GNT3)

OAS:	101113066(B3GNT3)

SSC:	100518473(B3GNT3)

CFR:	102512477(B3GNT3)

CDK:	105101856(B3GNT3)

BACU:

103012961(B3GNT3)

LVE:	103080965(B3GNT3)

OOR:	101282171(B3GNT3)

DLE:	111166586(B3GNT3)

PCAD:

102989486(B3GNT3)

ECB:	100146466(B3GNT3)

EPZ:	103542315(B3GNT3)

EAI:	106830835(B3GNT3)

MYB:	102254606(B3GNT3)

MYD:	102767713(B3GNT3)

MNA:	107535321(B3GNT3)

HAI:	109382386(B3GNT3)

DRO:	112305269(B3GNT3)

PALE:

102882932(B3GNT3)

RAY:	107521817(B3GNT3)

MJV:	108388013(B3GNT3)

LAV:	100673587(B3GNT3)

MDO:	100010637(B3GNT3)

SHR:	100920625(B3GNT3)

PCW:	110223495(B3GNT3)

OAA:	100086714(B3GNT3)

GGA:	112530425(B3GNT3)

MGP:	100549756(B3GNT3)

CJO:	107325454(B3GNT3)

NMEL:

110388735(B3GNT3)

ACYG:

106048207(B3GNT3)

FPG:	106112886(B3GNT3)

FCH:	102054592(B3GNT3)

CLV:	102095902(B3GNT3)

EGZ:	104124478(B3GNT3)

NNI:	104013696(B3GNT3)

AAM:

106486607

ASN:	102373956(B3GNT3)

AMJ:	106737906(B3GNT3)

PSS:	102443521(B3GNT3)

CMY:	102944348(B3GNT3)

CPIC:

101936651(B3GNT3)

PVT:	110072506(B3GNT3)

PBI:	103061136(B3GNT3)

PMUR:

107285005 107285006 107296837(B3GNT3) 114922368

TSR:

106556824

PMUA:

114588656(B3GNT3)

GJA:	107120629(B3GNT3)

XLA:	108703715 108711807 734264

XTR:	100145701(b3gnt3.2) 100380117(b3gnt3.1) 100494334 101733889 116409851

NPR:	108790801 108793753 108803023

DRE:	100002020(b3gnt3.2) 561172(b3gnt3.4)

SRX:

107755313

SANH:

107665060 107700302(b3gnt3)

SGH:

107599054

CCAR:

109083431 109099585

IPU:	108265513(b3gnt3) 108265849 108266016 108266017

PHYP:

113525137(b3gnt3)

AMEX:

103026573(b3gnt3)

EEE:	113587291 113587962

TRU:	101072648(b3gnt3)

TNG:	GSTEN00019098G001 GSTEN00019099G001 GSTEN00019100G001

LCO:	104935378 109139021(b3gnt3) 109139346 109139347

NCC:

104958016

MZE:	101474209 105940915

ONL:	100700249 100710618 102077342(b3gnt3)

OLA:	101163432 101173163 101173646 101175309

XMA:	102222047 102226121 102235218(b3gnt3) 111606492

XCO:	114137712 114137713 114150430 114151525 114152730

PRET:

103463427 103463431 103475708 103476783

CVG:	107095630 107103340(b3gnt3) 107103343 107103671 107104833

NFU:	107376009 107386572 107392806

KMR:	108234252 108234256 108243045 108247418

ALIM:

106519220 106519221 106533410

AOCE:

111576549(b3gnt3)

CSEM:

103395334 103395738 103395759 103399385

POV:	109636623(b3gnt3)

LCF:	108881191 108893170

SDU:	111218271(b3gnt3) 111235274

SLAL:

111669970(b3gnt3) 111670022

HCQ:	109519123(b3gnt3)

BPEC:

110160181

MALB:

109958356

SASA:

106573871(b3gnt3) 106578060 106613654

OTW:

112250760

SALP:

111953012(b3gnt3)

ELS:	105007499 105011432(b3gnt3) 105031420

SFM:	108930515(b3gnt3)

PKI:	111860178(b3gnt3)

LCM:	102345207(B3GNT3)

CMK:	103181970(b3gnt3)

RTP:

109918138

ISC:	IscW_ISCW004061

» show all

Reference

1 [PMID:6226356]

Authors

Brockhausen I, Rachaman ES, Matta KL, Schachter H.

Title

The separation by liquid chromatography (under elevated pressure) of phenyl, benzyl, and O-nitrophenyl glycosides of oligosaccharides. Analysis of substrates and products for four N-acetyl-D-glucosaminyl-transferases involved in mucin synthesis.

Journal

Carbohydr Res 120:3-16 (1983)
DOI:10.1016/0008-6215(83)88002-1

Reference

2 [PMID:11042166]

Authors

Shiraishi N, Natsume A, Togayachi A, Endo T, Akashima T, Yamada Y, Imai N, Nakagawa S, Koizumi S, Sekine S, Narimatsu H, Sasaki K

Title

Identification and characterization of three novel beta 1,3-N-acetylglucosaminyltransferases structurally related to the beta 1,3-galactosyltransferase family.

Journal

J Biol Chem 276:3498-507 (2001)
DOI:10.1074/jbc.M004800200

Sequence

[hsa:10331]

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.146

IUBMB Enzyme Nomenclature:

2.4.1.146

ExPASy - ENZYME nomenclature database:

2.4.1.146

BRENDA, the Enzyme Database:

2.4.1.146

CAS:	87927-99-9

DBGET integrated database retrieval system