KEGG ENZYME: 2.4.1.146

ENZYME: 2.4.1.146

Entry

EC 2.4.1.146                Enzyme

Name

beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase;
O-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II;
uridine diphosphoacetylglucosamine-mucin beta(1->3)-acetylglucosaminyltransferase (elongating);
elongation 3beta-GalNAc-transferase;
UDP-N-acetyl-D-glucosamine:O-glycosyl-glycoprotein (N-acetyl-D-glucosamine to beta-D-galactose of beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R) beta-1,3-N-acetyl-D-glucosaminyltransferase;
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->3)-[N-acetyl-D-glucosaminyl-(1->6)]-N-acetyl-D-galactosaminyl-R 3-beta-N-acetyl-D-glucosaminyltransferase;
B3GNT3 (gene name)

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-N-acetyl-alpha-D-glucosamine:3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] 3-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)

Reaction(IUBMB)

UDP-N-acetyl-alpha-D-glucosamine + 3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] = UDP + 3-O-{N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] [RN:R04636 R05901]

Reaction(KEGG)

R04636 R05901(G)

Substrate

UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein]

Product

UDP [CPD:C00015];
3-O-{N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein]

Comment

The enzyme catalyses the addition of N-acetyl-alpha-D-glucosamine to the core 2 structure of O-glycans.

History

EC 2.4.1.146 created 1984, modified 2018

Orthology

K07970

beta-1,3-N-acetylglucosaminyltransferase 3

Genes

HSA:	10331(B3GNT3)

PTR:	455847(B3GNT3)

PPS:	100982986(B3GNT3)

GGO:	101151557(B3GNT3)

PON:	100433243(B3GNT3)

PPYG:

129020968(B3GNT3)

NLE:	100596245(B3GNT3)

HMH:	116478680(B3GNT3)

SSYN:

129460854(B3GNT3)

MCC:	114674034(B3GNT3) 719221

MCF:	102134304(B3GNT3)

MTHB:

126942289

MNI:	105489382(B3GNT3)

CSAB:

103234153(B3GNT3)

CATY:

105601677(B3GNT3)

PANU:

101005136(B3GNT3)

TGE:	112613343(B3GNT3)

MLEU:

105546800(B3GNT3)

RRO:	104660961(B3GNT3)

RBB:	108534279(B3GNT3)

TFN:	117076459(B3GNT3)

PTEH:

111554311(B3GNT3)

CANG:

105500325(B3GNT3)

CJC:	100404385(B3GNT3)

SBQ:	101033879(B3GNT3)

CIMI:

108284529(B3GNT3)

ANAN:

105711057(B3GNT3)

MMUR:

105854991(B3GNT3)

LCAT:

123639536(B3GNT3)

PCOQ:

105818103(B3GNT3)

OGA:	100961131(B3GNT3)

MMU:	72297(B3gnt3)

MCAL:

110299618(B3gnt3)

MPAH:

110337004(B3gnt3)

RNO:	290638(B3gnt3)

MCOC:

116071470(B3gnt3)

ANU:	117721784(B3gnt3)

ASYL:

127668579(B3gnt3)

MUN:	110549208(B3gnt3)

CGE:	100773213(B3gnt3)

MAUA:

101827726(B3gnt3)

PROB:

127217283(B3gnt3)

PLEU:

114687946(B3gnt3)

MORG:

121462729(B3gnt3)

MFOT:

126491824

AAMP:

119811571(B3gnt3)

NGI:	103726889(B3gnt3)

HGL:	101713138(B3gnt3)

CPOC:

100713343(B3gnt3)

CCAN:

109698339(B3gnt3)

DSP:	122100853(B3gnt3)

DMER:

138817547(B3gnt3)

PLOP:

125347715(B3gnt3)

NCAR:

124969063

MMMA:

107145055(B3gnt3)

OPI:	101527594(B3GNT3)

TUP:	102472378(B3GNT3)

GVR:	103593680(B3GNT3)

CFA:	484831(B3GNT3)

CLUD:

112666725(B3GNT3)

VVP:	112913174(B3GNT3)

VLG:	121494311(B3GNT3)

NPO:	129522419(B3GNT3)

AML:	100473756(B3GNT3)

UMR:	103675168(B3GNT3)

UAH:	113256690(B3GNT3)

UAR:	123780002(B3GNT3)

ELK:

111144118

LLV:

125082431

MPUF:

101688106(B3GNT3)

MNP:	132009753(B3GNT3)

MLK:	131823742(B3GNT3)

NVS:	122909602(B3GNT3)

ORO:	101368545(B3GNT3)

EJU:	114226652(B3GNT3)

ZCA:	113917507(B3GNT3)

MLX:	118005244(B3GNT3)

NSU:	110574500(B3GNT3)

LWW:	102728088(B3GNT3)

FCA:	101092089(B3GNT3)

PYU:	121010990(B3GNT3)

PCOO:

112852386(B3GNT3)

PBG:	122487077(B3GNT3)

PVIV:

125161003(B3GNT3)

LRUF:

124524160

LGF:	123607391(B3GNT3)

AJU:

106969093

PTG:	102965987(B3GNT3)

PPAD:

109247961(B3GNT3)

PUC:

125929220

PLEZ:

122214155(B3GNT3)

HHV:	120239150(B3GNT3)

BTA:	784997(B3GNT3)

BOM:	102281061(B3GNT3)

BIU:	109560822(B3GNT3)

BBUB:

102400108(B3GNT3)

BBIS:

104992682(B3GNT3)

CHX:	102171777(B3GNT3)

OAS:	101113066(B3GNT3)

BTAX:

128051590(B3GNT3)

CSUM:

138086125(B3GNT3)

ODA:	120864228(B3GNT3)

CCAD:

122440724(B3GNT3)

MREE:

136165344(B3GNT3)

OVR:	110140406(B3GNT3)

MBEZ:

129559375(B3GNT3)

SSC:	100518473(B3GNT3)

CFR:	102512477(B3GNT3)

CBAI:

105082559(B3GNT3)

CDK:	105101856(B3GNT3)

VPC:	102538202(B3GNT3)

BACU:

103012961(B3GNT3)

BMUS:

118892972(B3GNT3)

LVE:	103080965(B3GNT3)

OOR:	101282171(B3GNT3)

LALB:

132517466(B3GNT3)

GMF:	115865391(B3GNT3)

DDP:	132422917(B3GNT3)

DLE:	111166586(B3GNT3)

PCAD:

102989486(B3GNT3)

PSIU:

116750777(B3GNT3)

PPHC:

136121272(B3GNT3)

NASI:

112414694(B3GNT3)

ECB:	100146466(B3GNT3)

EPZ:	103542315(B3GNT3)

EAI:	106830835(B3GNT3)

MYB:	102254606(B3GNT3)

MYD:	102767713(B3GNT3)

MMYO:

118658722(B3GNT3)

MLF:	102428358(B3GNT3)

MDT:	132234825(B3GNT3)

MYUM:

139011124(B3GNT3)

PKL:	118722609 118725871(B3GNT3)

EFUS:

103299580(B3GNT3)

MNA:	107535321(B3GNT3)

DRO:	112305269(B3GNT3)

SHON:

118975180 118975182

AJM:	119043950 119046818

PDIC:

114503731 114503735

PHAS:

123828315 123828458

MMF:	118616497(B3GNT3)

PPAM:

129074834(B3GNT3)

HAI:	109382386(B3GNT3)

RFQ:	117038056(B3GNT3)

PALE:

102882932(B3GNT3)

PGIG:

120606538(B3GNT3)

PVP:	105296673(B3GNT3)

RAY:	107521817(B3GNT3)

MJV:	108388013(B3GNT3)

TOD:	119241108(B3GNT3)

SARA:

101553979(B3GNT3)

SFUM:

130028945(B3GNT3)

SETR:

126030205(B3GNT3)

LAV:	100673587(B3GNT3)

ETF:	101640855(B3GNT3)

MDO:	100010637(B3GNT3)

GAS:	123232263(B3GNT3)

SHR:	100920625(B3GNT3)

AFZ:

127545239

PCW:	110223495(B3GNT3)

TVP:	118834173(B3GNT3)

PBRV:

138142838(B3GNT3)

OAA:	100086714(B3GNT3)

TACU:

119919971(B3GNT3)

GGA:	112530425(B3GNT3)

PCOC:

116235881

MGP:	100549756(B3GNT3)

CJO:	107325454(B3GNT3)

TPAI:

128088910(B3GNT3)

LMUT:

125687729(B3GNT3)

NMEL:

110388735(B3GNT3)

ACYG:

106048207(B3GNT3)

CATA:

118259940(B3GNT3)

FPG:	106112886(B3GNT3)

FCH:	102054592(B3GNT3)

TALA:

116963233 122153644

ACHC:

115348859(B3GNT3)

HLE:	104829181(B3GNT3)

AGEN:

126041852

HHAR:

128148306(B3GNT3)

CSTI:

104562350

MNB:

103773667

PPUS:

135192056(B3GNT3)

AVIT:

104271044

BRHI:

104489191

EGZ:

104124478

NNI:	104013696(B3GNT3)

PCRI:

104035360

AFOR:

103899393(B3GNT3)

CBOY:

140643723(B3GNT3)

CLV:	102095902(B3GNT3)

CNIC:

135999281(B3GNT3)

MUI:

104540502

CMAC:

104484684

TEO:

104375381

ACAR:

104527704

CVF:

104287995

RTD:	128900523(B3GNT3)

CRID:

134526380 134526445

AAM:

106486607

AROW:

112965649(B3GNT3)

NPD:	112949098(B3GNT3)

DNE:	112992748(B3GNT3)

ASN:	102373956(B3GNT3)

AMJ:	106737906(B3GNT3)

CPOO:

109318684(B3GNT3)

GGN:	109302437(B3GNT3)

PSS:	102443521(B3GNT3)

CMY:	102944348(B3GNT3)

CCAY:

125627120(B3GNT3)

DCC:	119848387(B3GNT3)

CPIC:

101936651(B3GNT3)

TST:	117869061(B3GNT3)

CABI:

116833222(B3GNT3)

MRV:	120391923(B3GNT3)

ASAO:

132763922

PVT:	110072506(B3GNT3)

SUND:

121936496(B3GNT3)

PBI:	103061136(B3GNT3)

PMUR:

107285005 107285006 107296837(B3GNT3) 114922368

CTIG:

120299531(B3GNT3)

TSR:

106556824

PGUT:

117670585(B3GNT3)

PCAE:

139308429(B3GNT3)

APRI:

131188362(B3GNT3)

PTEX:

113451171(B3GNT3)

NSS:

113428574

VKO:	123018694(B3GNT3)

PMUA:

114588656(B3GNT3)

PRAF:

128400762 128405712(B3GNT3)

ZVI:	118087801(B3GNT3)

HCG:	128341521(B3GNT3) 128343789

GJA:	107120629(B3GNT3)

STOW:

125432362(B3GNT3)

EMC:	129331138(B3GNT3)

XLA:	108703715(b3gnt3.2.S) 108711807 734264(b3gnt3.2.L)

XTR:	100145701(b3gnt3.2) 100380117(b3gnt3.1) 100494334 101733889 116409851

NPR:	108790801 108793753 108803023

RTEM:

120909662 120909687 120914440 120933672 120933692

ACAT:

141103557 141103564 141103573 141107219 141133625 141133632

BBUF:

120989390(B3GNT3) 121009144

BGAR:

122919843 122920562(B3GNT3)

DTC:	141072556 141075454

PWL:	138266856 138267541(B3GNT3)

MUO:	115480102(B3GNT3) 115480542

GSH:	117350860 117365631(B3GNT3) 117365632 117365798

DRE:	100002020(b3gnt3.2) 561172(b3gnt3.4)

SRX:

107755313

SANH:

107665060 107700302(b3gnt3)

SGH:

107599054

CCAR:

109063583 109083431 109099585

CAUA:

113049247 113077616 113099571

CGIB:

127963990 128018881

PTET:

122350844(b3gnt3.4)

LROH:

127159074 127169401 127169402

GRF:

141287441

OMC:	131545379(b3gnt3.4) 131546315

PPRM:

120474104(b3gnt3.4) 120474110

RKG:	130091580 130091660

MAMB:

125279505 125280378 125280419 125280600

CIDE:

127517639

CERY:

137026271 137026272 137026910

MASI:

127414726 127423798 127423799

TROS:

130568603(b3gnt3.4)

TDW:	130406944(b3gnt3.4)

MANU:

129414010(b3gnt3.4)

PDAB:

135730455(b3gnt3.4)

IPU:	108265513 108265514 108265849 108266016 108266017 128632865

IFU:	128607259 128607260 128608235 128608236 128608237 128608240

PHYP:

113525137(b3gnt3)

SMEO:

124399388 124400092 124400431

TFD:	113634256 113634260 113642616 113642617 113642618 113642619 113642620 125146324

TVC:	132853478 132853519 132853763 132854077

CGAR:

128511243 128529697 128530046 128530073 128530075

NGRA:

132892666 132892667 132892668 132893473

TRN:	134318542 134318543

AMEX:

103026573

CMAO:

118812996 118812997 118812998 118813000 118813001 118814006 118814119 118814120 118817469

EEE:	113587291 113587962

CHAR:

105910708

SPIL:

134102687

TRU:	101072648(b3gnt3)

TFS:	130528986 130528987 130528989

TNG:	GSTEN00019098G001 GSTEN00019099G001 GSTEN00019100G001

LCO:	104935378 109139021(b3gnt3) 109139346 109139347

NCC:

104958016

TBEN:

117496023

CGOB:

115007161(b3gnt3)

PGEO:

117444957

GACU:

117544206 117544235 117544258

EMAC:

134869600

ELY:	117253529 117263620 117264498

EFO:	125896293 125898369

PLEP:

121941118 121952728

SLUC:

116055449

ECRA:

117950580

ESP:	116696007(b3gnt3)

PFLV:

114561872(b3gnt3)

GAT:

120824339

PPUG:

119217180

AFB:	129092171 129094357 129113928

CLUM:

117729613

PSWI:

130191696

CANA:

137793794

CVE:	137870356 137877389 137890726

MSAM:

119900537 119900538 119900539 119917039

SCHU:

122878174 122878175 122878176 122884902

CUD:	121512695 121519212 121519214 121519215

SPUL:

138604033

LMIX:

132966730 132979987 132988701 132988742

ALAT:

119028474 119028475 119029000

ASTR:

137599018 137599216

MZE:	101474209 105940915

ONL:	100700249 100710618 102077342(b3gnt3)

OAU:	116328445 116328458 116331915 116333906

OLA:	101163432 101173163 101173646 101175309

OML:	112138882 112139123 112142676 112142717 112150918 112150945

CSAI:

133457845 133458528 133459734 133460319

XMA:	102222047 102226121 102235218(b3gnt3) 111606492

XCO:	114137712 114137713 114150430 114151525 114152730

XHE:	116713044 116713045 116725213 116725996 116727933

PRET:

103463427 103463431 103475708 103476783

PFOR:

103129919 103129923 103133540 103133955

PLAI:

106937802 106937900 106947686 106955559

PMEI:

106906127 106914022 106914028 106914127

GAF:	122838849 122838850 122842547 122845157

PPRL:

129360191 129360192 129360194 129368864 129375276 129379706

CVG:	107095630 107103340(b3gnt3) 107103343 107103671 107104833

CTUL:

119773651 119786226 119786238 119787603 119787742 119790866

GMU:	124858982 124873366 124874498 124876850

NFU:	107376009 107386572 107392806

KMR:	108234252 108234256 108243045 108247418 119616708

ALIM:

106519220 106519221 106533410

NWH:	119407675 119407676 119420122 119430271

AOCE:

111576549 111584703 111584710 111584717 111584724 111584732

EJA:	138200577 138200578 138200579

BFRE:

138623045 138623046

MCEP:

125009341 125009989 125018706

CSEM:

103395334 103395738 103395759 103399385

POV:	109636623(b3gnt3)

SSEN:

122780488 122781014 122781015

SSOE:

131465087 131466218 131466264

HHIP:

117760258 117760259 117760260 117760575 117760577

HSP:	118120691 118121259 118121338 118121467

PPLT:

128447730 128447731 128447732 128447768

SMAU:

118300122 118317900

LCF:	108881191 108881192 108893170

SDU:	111218271(b3gnt3) 111235274

SLAL:

111669970(b3gnt3) 111670022

XGL:	120790634 120791330 120802899

HCQ:	109519123(b3gnt3)

SSCV:

125966005

SBIA:

133503367

PEE:

133406257

PTAO:

133480587

BPEC:

110160181

ENY:	140354230 140354372

MALB:

109958356

BSPL:

114852890 114854074 114854075 114854125 114854126 114854127 114854343 114854344 114854629 114854649

SJO:	128362250 128370937

SSCO:

133984942 133985223 133994364

GMH:	115537417 115537655 115556108(b3gnt3)

SASA:

106573871(b3gnt3) 106578060 106613654

STRU:

115151709

OTW:	112250760(b3gnt3.4) 112252159

OMY:	110496754 110524171

OGO:	123996683 123998437 124007935

ONE:

115109173

OKI:

109889149

OKE:	118383428 118401436

OMM:	135512671 135524624

OCLA:

139415379

SALP:

111953012(b3gnt3)

SNH:	120053736 120054432

CCLU:

121577321 123481027

ELS:	105007499 105011432(b3gnt3) 105031420

OEP:	134010864 134010865 134011130 134011334 134011349 134011372

SFM:	108930515(b3gnt3)

PKI:	111860178(b3gnt3)

AANG:

118221742 118226430

AROT:

135239318 135252908

CCOG:

133127072 133142347

LOC:	102687306(b3gnt3)

PSPA:

121301356 121301670

ARUT:

117401222 117966163 117966195 117966263 117966282 117966310 117968039 117972057

PSEX:

120515032 120537847

LCM:	102345207(B3GNT3)

CMK:

103181970

RTP:

109918138

CPLA:

122565439

HOC:

132828963

LERI:

129711209 129716043

HAKA:

140720568 140720573 140720598 140720823 140720824 140721296 140721499 140721532 140721751 140722563 140722621 140722870 140723051 140723150 140723422 140723424 140724444

PMRN:

116943159 116950398 116950418 116951898

LRJ:	133342421 133350119 133352502

MSEX:

115456046

PPOT:

106102791

» show all

Reference

1 [PMID:6226356]

Authors

Brockhausen I, Rachaman ES, Matta KL, Schachter H.

Title

The separation by liquid chromatography (under elevated pressure) of phenyl, benzyl, and O-nitrophenyl glycosides of oligosaccharides. Analysis of substrates and products for four N-acetyl-D-glucosaminyl-transferases involved in mucin synthesis.

Journal

Carbohydr Res 120:3-16 (1983)
DOI:10.1016/0008-6215(83)88002-1

Reference

2 [PMID:11042166]

Authors

Shiraishi N, Natsume A, Togayachi A, Endo T, Akashima T, Yamada Y, Imai N, Nakagawa S, Koizumi S, Sekine S, Narimatsu H, Sasaki K

Title

Identification and characterization of three novel beta 1,3-N-acetylglucosaminyltransferases structurally related to the beta 1,3-galactosyltransferase family.

Journal

J Biol Chem 276:3498-507 (2001)
DOI:10.1074/jbc.M004800200

Sequence

[hsa:10331]

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.146

IUBMB Enzyme Nomenclature:

2.4.1.146

ExPASy - ENZYME nomenclature database:

2.4.1.146

BRENDA, the Enzyme Database:

2.4.1.146

CAS:	87927-99-9

All links

Gene (104)   
   MGENES (63)   
   RefGene (41)   
Protein sequence (10)   
   UniProt (2)   
   SWISS-PROT (2)   
   RefSeq(pep) (6)   
DNA sequence (7)   
   RefSeq(nuc) (7)   
Protein domain (1)   
   InterPro (1)   
All databases (122)   

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