KEGG   ENZYME: 2.4.1.150Help
Entry
EC 2.4.1.150                Enzyme                                 

Name
N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase;
GCNT2 (gene name);
GCNT3 (gene name);
IGnT;
I-branching beta1,6-N-acetylglucosaminyltransferase;
N-acetylglucosaminyltransferase;
uridine diphosphoacetylglucosamine-acetyllactosaminide beta1->6-acetylglucosaminyltransferase;
Galbeta1->4GlcNAc-R beta1->6 N-acetylglucosaminyltransferase;
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide beta-1,6-N-acetyl-D-glucosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-glucosamine:beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminide 6-beta-N-acetylglucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-R = UDP + beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-[beta-D-GlcNAc-(1->6)]-beta-D-Gal-(1->4)-beta-D-GlcNAc-R [RN:R11879]
Reaction(KEGG)
R11879 > R06189(G);
(other) R02790 R04654 R06013(G)
Show
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-R [CPD:C21742]
Product
UDP [CPD:C00015];
beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-[beta-D-GlcNAc-(1->6)]-beta-D-Gal-(1->4)-beta-D-GlcNAc-R [CPD:C21743]
Comment
The enzyme acts on poly-N-acetyllactosamine [glycan chains of beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine units connected by beta(1,3) linkages] attached to proteins or lipids. It transfers a GlcNAc residue by beta(1,6)-linkage to galactosyl residues close to non-reducing terminals, introducing a branching pattern known as I branching.
History
EC 2.4.1.150 created 1984 (EC 2.4.1.164 created 1989, incorporated 2016), modified 2017
Pathway
ec00601  Glycosphingolipid biosynthesis - lacto and neolacto series
ec01100  Metabolic pathways
Orthology
K00742  N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase
Genes
HSA: 2651(GCNT2)
PTR: 462428(GCNT2)
PPS: 100979555(GCNT6) 100983940(GCNT2)
GGO: 101132299 101133045(GCNT2) 109027295
PON: 100173016(GCNT2)
NLE: 100600035 100601598(GCNT2)
MCC: 697468(GCNT2)
MCF: 102131882(GCNT2) 107129630 107129632
CSAB: 103222180 103222182 103222183
RRO: 104659228 104671974(GCNT2)
MMU: 14538(Gcnt2)
RNO: 306860(Gcnt2) 689092(Gcnt6)
NGI: 103732899(Gcnt2) 103732948
CCAN: 109700881(Gcnt2) 109700883
OCU: 100356713(GCNT2) 103349711
TUP: 102485510(GCNT2)
CFA: 488215
UMR: 103664926(GCNT2)
FCA: 101094925(GCNT2) 109491352
PTG: 102972600(GCNT2)
AJU: 106972037(GCNT2)
BIU: 109577218
CHX: 100861326(GCNT2)
OAS: 101106554(GCNT2)
SSC: 100152566
CFR: 102504922 102520753(GCNT2)
CDK: 105101211
BACU: 103007744 103010651(GCNT2)
ECB: 100063291
EPZ: 103559596 103559598(GCNT2)
EAI: 106833434(GCNT2)
MYB: 102245671 102245970(GCNT2)
MYD: 102770362(GCNT2) 102770938
HAI: 109377537(GCNT2)
RSS: 109458559(GCNT2)
PALE: 102877728(GCNT2)
MDO: 100027080(GCNT2)
SHR: 100925756(GCNT2)
GGA: 428479(GCNT2)
MGP: 100539032
CJO: 107309590(GCNT2)
APLA: 101795771(GCNT2) 106020659
ACYG: 106034848 106041798(GCNT2)
GFR: 106631903(GCNT2)
FAB: 107603337(GCNT2)
PMAJ: 107215848(GCNT2)
CCW: 109145064(GCNT2)
FPG: 101918293(GCNT2)
FCH: 102057507(GCNT2)
CLV: 102086182(GCNT2)
EGZ: 104127709(GCNT2) 104133970
AAM: 106482558(GCNT2)
AMJ: 102575341(GCNT2)
PSS: 102458109(GCNT2)
CMY: 102930060(GCNT2) 102931620
CPIC: 101931709(GCNT2) 101938597
PBI: 103065974(GCNT2)
GJA: 107111763(GCNT2)
XLA: 108718735 432177 432275(gcnt2.L)
XTR: 100038221(gcnt2) 100491539(gcnt2)
LCM: 102366551(GCNT2)
CMK: 103184570
SPU: 579029
CRG: 105347402
ADF: 107329241
AQU: 105315875
 » show all
Taxonomy
Reference
1  [PMID:6219989]
  Authors
van den Eijnden DH, Winterwerp H, Smeeman P, Schiphorst WE.
  Title
Novikoff ascites tumor cells contain N-acetyllactosaminide beta 1 leads to 3 and beta 1 leads to 6 N-acetylglucosaminyltransferase activity.
  Journal
J Biol Chem 258:3435-7 (1983)
Reference
2  [PMID:6238026]
  Authors
Basu M, Basu S.
  Title
Biosynthesis in vitro of Ii core glycosphingolipids from neolactotetraosylceramide by beta 1-3- and beta 1-6-N-acetylglucosaminyltransferases from mouse T-lymphoma.
  Journal
J Biol Chem 259:12557-62 (1984)
Reference
3  [PMID:6490658]
  Authors
Piller F, Cartron JP, Maranduba A, Veyrieres A, Leroy Y, Fournet B
  Title
Biosynthesis of blood group I antigens. Identification of a UDP-GlcNAc:GlcNAc beta 1-3Gal(-R) beta 1-6(GlcNAc to Gal) N-acetylglucosaminyltransferase in hog gastric mucosa.
  Journal
J Biol Chem 259:13385-90 (1984)
Reference
4  [PMID:7579796]
  Authors
Bierhuizen MF, Maemura K, Kudo S, Fukuda M
  Title
Genomic organization of core 2 and I branching beta-1,6-N-acetylglucosaminyltransferases. Implication for evolution of the beta-1,6-N-acetylglucosaminyltransferase gene family.
  Journal
Glycobiology 5:417-25 (1995)
DOI:10.1093/glycob/5.4.417
Reference
5  [PMID:10092606]
  Authors
Ujita M, McAuliffe J, Suzuki M, Hindsgaul O, Clausen H, Fukuda MN, Fukuda M
  Title
Regulation of I-branched poly-N-acetyllactosamine synthesis. Concerted actions by I-extension enzyme, I-branching enzyme, and beta1,4-galactosyltransferase I.
  Journal
J Biol Chem 274:9296-304 (1999)
DOI:10.1074/jbc.274.14.9296
Reference
6  [PMID:9915862]
  Authors
Yeh JC, Ong E, Fukuda M
  Title
Molecular cloning and expression of a novel beta-1, 6-N-acetylglucosaminyltransferase that forms core 2, core 4, and I branches.
  Journal
J Biol Chem 274:3215-21 (1999)
DOI:10.1074/jbc.274.5.3215
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.150
IUBMB Enzyme Nomenclature: 2.4.1.150
ExPASy - ENZYME nomenclature database: 2.4.1.150
BRENDA, the Enzyme Database: 2.4.1.150
CAS: 85638-40-0

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