KEGG   ENZYME: 2.4.1.150
Entry
EC 2.4.1.150                Enzyme                                 
Name
N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase;
GCNT2 (gene name);
GCNT3 (gene name);
IGnT;
I-branching beta1,6-N-acetylglucosaminyltransferase;
N-acetylglucosaminyltransferase;
uridine diphosphoacetylglucosamine-acetyllactosaminide beta1->6-acetylglucosaminyltransferase;
Galbeta1->4GlcNAc-R beta1->6 N-acetylglucosaminyltransferase;
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide beta-1,6-N-acetyl-D-glucosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-N-acetyl-alpha-D-glucosamine:beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminide 6-beta-N-acetylglucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-R = UDP + beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-[beta-D-GlcNAc-(1->6)]-beta-D-Gal-(1->4)-beta-D-GlcNAc-R [RN:R11879]
Reaction(KEGG)
R11879 > R06189(G);
(other) R02790 R04654 R06013(G)
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-R [CPD:C21742]
Product
UDP [CPD:C00015];
beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-[beta-D-GlcNAc-(1->6)]-beta-D-Gal-(1->4)-beta-D-GlcNAc-R [CPD:C21743]
Comment
The enzyme acts on poly-N-acetyllactosamine [glycan chains of beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine units connected by beta(1,3) linkages] attached to proteins or lipids. It transfers a GlcNAc residue by beta(1,6)-linkage to galactosyl residues close to non-reducing terminals, introducing a branching pattern known as I branching.
History
EC 2.4.1.150 created 1984 (EC 2.4.1.164 created 1989, incorporated 2016), modified 2017
Pathway
ec00601  Glycosphingolipid biosynthesis - lacto and neolacto series
ec01100  Metabolic pathways
Orthology
K00742  N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase
Genes
HSA2651(GCNT2)
PTR100610053 462428(GCNT2)
PPS100979555 100983940(GCNT2)
GGO101132299 101133045 109027295
PON100173016(GCNT2) 100449266
NLE100600035 100601598(GCNT2)
MCC697468(GCNT2)
MCF102131882(GCNT2)
MTHB126953872
CSAB103222182 103222183
CATY105572520 105572521
PANU101020180(GCNT2) 103883455 103883457
TGE112623439(GCNT2) 112623440
RRO104656690 104671974
RBB108521659 108521996 108522006 108522008 108528720
TFN117086715(GCNT2)
PTEH111521788(GCNT2)
CANG105526628(GCNT2) 105526629 105526805
CJC100389624(GCNT2) 100397136 100404131 100411905(GCNT2) 100895187
SBQ101030514(GCNT2) 101044056
CSYR103257198 103265522 103265534
MMUR105865110 105865180 105865181(GCNT2)
LCAT123638228 123638229 123638230
PCOQ105806482 105806483 105806487 105824853 105824854
OGA100944503 100956694 100957010 105886840
MMU14538(Gcnt2)
MCAL110307912
MPAH110333783(Gcnt2)
RNO102549059 306860(Gcnt2)
MCOC116081738
MUN110554603 110554604
CGE100760161 100763488 118238511
MAUA101824660(Gcnt2)
PLEU114692111 114692131
MORG121433997(Gcnt2)
MFOT126501511
AAMP119817452 119817453(Gcnt2)
NGI103732899(Gcnt2) 103732948
HGL101701828 101702179(Gcnt2) 101715209 101715564 101724068
CPOC100723536(Gcnt2)
CCAN109700881(Gcnt2) 109700883
DORD105985988 105985989
DSP122106996
NCAR124988358
OCU100356713 103349711
OPI101533291(GCNT2) 118758264
TUP102469358 102485510(GCNT2)
GVR103602336(GCNT2)
CFA488215(GCNT2)
CLUD112658449(GCNT2)
VVP112920550 112920551
VLG121499590(GCNT2)
AML100468405(GCNT2) 117802286
UMR103664926(GCNT2)
UAH113264440 113264441
UAR123801609 123801610
ELK111156684 111156685 111156720
LLV125102205 125102206
MPUF101681334(GCNT2)
ORO101380605 101383306 101383589
EJU114212530(GCNT2) 114212531
ZCA113926599(GCNT2)
MLX118027214 118027450
NSU110586640
LWW102727112(GCNT2) 102728644
FCA101094925(GCNT2) 109491352
PYU121020542(GCNT2) 121020543
PBG122489246(GCNT2)
LRUF124528243
PTG102972600(GCNT2)
PPAD109249650(GCNT2)
AJU106972037 113592392
HHV120231663(GCNT2) 120231665
BTA100848875 112443869 520336
BOM102276215 102276507 102277629 102277917
BIU109577218
BBUB102401082(GCNT2)
BBIS104985613 104985614
CHX100861326
OAS101106554
ODA120869900 120869903
CCAD122429708(GCNT2) 122429987
SSC100152566 102157807 110255306
CFR102520753(GCNT2)
CBAI105061625 105061626
CDK105101211(GCNT2)
VPC102531012(GCNT2)
BACU103007744 103010651(GCNT2)
LVE103088671 103088938 103089209
OOR101271548 101271797 101282851
DLE111181352(GCNT2) 111181354
PCAD102980967(GCNT2)
PSIU116761802(GCNT2) 116761803
ECB100063291
EPZ103559596 103559598(GCNT2)
EAI106833434(GCNT2)
MYB102245671 102245970(GCNT2)
MYD102770362(GCNT2) 102770649 102770938
MMYO118675060(GCNT2) 118675062
MLF102426091 111823016
MNA107530399 107530416 107530417
PKL118724427 118724428
HAI109377537(GCNT2)
DRO112297544(GCNT2)
SHON118986439(GCNT2)
AJM119034524 119034528 119034529
PDIC114496371 118500744
PHAS123820762 123820763
MMF118627206(GCNT2) 118627207
RFQ117026868(GCNT2)
PALE102877728 102898828
PGIG120598505 120598937(GCNT2) 120598938
PVP105300842 105300843
RAY107497201 107497202 107497203
MJV108396557(GCNT2)
TOD119251600 119251601 119253232
SARA101541384 101542179
LAV100662133 100674841 100675128 100675407 100675689
TMU101352763 101355412 101355931 101356187 101356441
ETF101638403 101639346 101647352(GCNT2)
DNM101424024(GCNT2) 111760113
MDO100027080(GCNT2) 100027097 100027131
GAS123231849 123254659 123254681
SHR100925756 116420643 116420653
PCW110191997 110192005 110224059 110224060 110224061
OAA100084006
GGA428479(GCNT2)
PCOC116226449 116226450 116241361
MGP100539032(GCNT2)
CJO107309590(GCNT2)
NMEL110394520 110394521
APLA101795771(GCNT2)
ACYG106034848 106041798
AFUL116485591 116485592
TGU115493561(GCNT2)
SCAN115483999(GCNT2)
PMOA120503483(GCNT2)
OTC121340411(GCNT2)
PRUF121357761(GCNT2)
GFR106631903(GCNT2)
FAB107603337(GCNT2)
PMAJ107215848(GCNT2)
CCAE111923931(GCNT2)
CCW109145064(GCNT2)
CBRC103613172(GCNT2)
ZAB102074701(GCNT2)
FPG101918293(GCNT2)
FCH102057507(GCNT2)
CLV102086182(GCNT2)
EGZ104127709(GCNT2) 104133970
NNI104021895 104021896
ACUN113476690(GCNT2)
TALA104363045(GCNT2)
PADL103919086(GCNT2)
AFOR103894363(GCNT2)
ACHC115352964(GCNT2)
HLE104835362(GCNT2)
AGEN126048702
GCL127012660 127012661
CCRI104155881 104166264
CSTI104562480(GCNT2)
EHS104505168 104515194
CMAC104474199(GCNT2)
MUI104543602(GCNT2)
LDI104351645(GCNT2)
MNB103773478(GCNT2)
OHA104326807(GCNT2)
NNT104405472(GCNT2)
PGUU104468166(GCNT2)
ACAR104527893(GCNT2)
CPEA104391656(GCNT2)
AVIT104276295(GCNT2)
CVF104285716 104286796
CUCA104062151(GCNT2)
TEO104371450
BRHI104499582(GCNT2)
AAM106482558(GCNT2)
AROW112965365(GCNT2)
NPD112947304(GCNT2)
TGT104566593 104567614(GCNT2)
DNE112991986(GCNT2)
SCAM104146145(GCNT2)
ASN102373136 102374392 106722091
AMJ102575341(GCNT2)
GGN109298522(GCNT2)
PSS102458109 102458349
CMY102930060(GCNT2)
CPIC101931709(GCNT2)
TST117871622 117873197
CABI116827966 116828588(GCNT2)
MRV120398881(GCNT2)
ACS100558219 100558415
PVT110080870 110080871
SUND121928724 121929100
PBI103065974
PMUR107282985(GCNT2)
CTIG120310841(GCNT2) 120315318
TSR106543763
PGUT117672478(GCNT2)
VKO123036189 123036194
PMUA114600911 114601371 114601373
ZVI118090127 118090174 118090429(GCNT2)
GJA107111763(GCNT2)
STOW125439305(GCNT2)
XLA108718735 432177(gcnt2.2.S) 432275(gcnt2.2.L)
XTR100038221(gcnt2.2) 100491539
NPR108788458 108788461
RTEM120940706 120940707
BBUF121001281 121001896
BGAR122938781 122939140
LOC102693956(gcnt2)
PSPA121313931
ARUT117394963
LCM102366551(GCNT2)
CMK103184570
RTP109937915
BFO118427322
CIN100175723
SCLV120330007
SPU115923646 578725 579029 763273
APLC110988200 110988201
AJC117100505
SKO100370818 100378232
LOALOAG_16642
HROHELRODRAFT_139499 HELRODRAFT_163385
LGILOTGIDRAFT_103000 LOTGIDRAFT_140870 LOTGIDRAFT_140905 LOTGIDRAFT_173237 LOTGIDRAFT_64655 LOTGIDRAFT_64882 LOTGIDRAFT_73453
PCAN112554326 112565138
BGT106058038 106058614 106060603
GAE121385625
HRF124125097
HRJ124253766 124269292 124270653
PMAX117318075
MMER123531800 123539824
OBI106872655
OVIT265_04703 T265_10156
NVE5511223
ATEN116290394
ADF107329241
AMIL114974181
XEN124440557
AQU105315875
 » show all
Reference
1  [PMID:6219989]
  Authors
van den Eijnden DH, Winterwerp H, Smeeman P, Schiphorst WE.
  Title
Novikoff ascites tumor cells contain N-acetyllactosaminide beta 1 leads to 3 and beta 1 leads to 6 N-acetylglucosaminyltransferase activity.
  Journal
J Biol Chem 258:3435-7 (1983)
Reference
2  [PMID:6238026]
  Authors
Basu M, Basu S.
  Title
Biosynthesis in vitro of Ii core glycosphingolipids from neolactotetraosylceramide by beta 1-3- and beta 1-6-N-acetylglucosaminyltransferases from mouse T-lymphoma.
  Journal
J Biol Chem 259:12557-62 (1984)
Reference
3  [PMID:6490658]
  Authors
Piller F, Cartron JP, Maranduba A, Veyrieres A, Leroy Y, Fournet B
  Title
Biosynthesis of blood group I antigens. Identification of a UDP-GlcNAc:GlcNAc beta 1-3Gal(-R) beta 1-6(GlcNAc to Gal) N-acetylglucosaminyltransferase in hog gastric mucosa.
  Journal
J Biol Chem 259:13385-90 (1984)
Reference
4  [PMID:7579796]
  Authors
Bierhuizen MF, Maemura K, Kudo S, Fukuda M
  Title
Genomic organization of core 2 and I branching beta-1,6-N-acetylglucosaminyltransferases. Implication for evolution of the beta-1,6-N-acetylglucosaminyltransferase gene family.
  Journal
Glycobiology 5:417-25 (1995)
DOI:10.1093/glycob/5.4.417
  Sequence
[hsa:2651]
Reference
5  [PMID:10092606]
  Authors
Ujita M, McAuliffe J, Suzuki M, Hindsgaul O, Clausen H, Fukuda MN, Fukuda M
  Title
Regulation of I-branched poly-N-acetyllactosamine synthesis. Concerted actions by I-extension enzyme, I-branching enzyme, and beta1,4-galactosyltransferase I.
  Journal
J Biol Chem 274:9296-304 (1999)
DOI:10.1074/jbc.274.14.9296
Reference
6  [PMID:9915862]
  Authors
Yeh JC, Ong E, Fukuda M
  Title
Molecular cloning and expression of a novel beta-1, 6-N-acetylglucosaminyltransferase that forms core 2, core 4, and I branches.
  Journal
J Biol Chem 274:3215-21 (1999)
DOI:10.1074/jbc.274.5.3215
  Sequence
[hsa:9245]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.150
IUBMB Enzyme Nomenclature: 2.4.1.150
ExPASy - ENZYME nomenclature database: 2.4.1.150
BRENDA, the Enzyme Database: 2.4.1.150
CAS: 85638-40-0

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