KEGG ENZYME: 2.4.1.232

ENZYME: 2.4.1.232

Entry

EC 2.4.1.232                Enzyme

Name

initiation-specific alpha-1,6-mannosyltransferase;
alpha-1,6-mannosyltransferase;
GDP-mannose:oligosaccharide 1,6-alpha-D-mannosyltransferase;
GDP-mannose:glycolipid 1,6-alpha-D-mannosyltransferase;
glycolipid 6-alpha-mannosyltransferase;
GDP-mannose:oligosaccharide 1,6-alpha-D-mannosyltransferase

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

GDP-mannose:oligosaccharide 6-alpha-D-mannosyltransferase

Reaction(IUBMB)

Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->6)-D-mannosyl-D-mannose linkage

Reaction(KEGG)

(other) R06986(G) R08599(G)

Comment

Requires Mn2+. In Saccharomyces cerevisiae, this enzyme catalyses an essential step in the outer chain elongation of N-linked oligosaccharides. Man8GlcNAc and Man9GlcNAc are equally good substrates.

History

EC 2.4.1.232 created 2004

Pathway

ec00513

Various types of N-glycan biosynthesis

ec01100

Metabolic pathways

Orthology

K05528

alpha 1,6-mannosyltransferase

K05529

mannan polymerase complexes MNN9 subunit

Genes

BFO:

118411725

HRO:	HELRODRAFT_153976

PCAN:

112561057

HRF:

124150828

HRJ:

124260835

CRG:	105320143 117685401

MMER:

123534147 123555091 123555664 123555901 123555902 123556100

OBI:	106868997 106869556

LAK:	106162184 106173809

QSU:	111992006 111999377 112005924 112013424 112026026 112026929 112027743

VCN:	VOLCADRAFT_96511

MNG:

MNEG_9768

CME:	CYME_CMT636C

GSL:	Gasu_17110 Gasu_59940

SCE:	YGL038C(OCH1) YPL050C(MNN9)

SEUB:

DI49_1929 DI49_5408

SPAO:

SPAR_G01980 SPAR_P02160

AGO:	AGOS_AFR465C AGOS_AGL259C

ERC:	Ecym_1409 Ecym_2550

KLA:	KLLA0_C04125g KLLA0_E12849g KLLA0_F22220g

KMX:	KLMA_10184(och1) KLMA_40267(OCH1) KLMA_70378(MNN9)

LTH:	KLTH0E11616g KLTH0H09064g

VPO:	Kpol_1026p11 Kpol_1031p78

ZRO:	ZYRO0F08492g ZYRO0G09284g

CGR:	CAGL0A01738g CAGL0L12804g

NCS:	NCAS_0A01880(NCAS0A01880) NCAS_0B02440(NCAS0B02440) NCAS_0C02330(NCAS0C02330)

NDI:	NDAI_0C05250(NDAI0C05250) NDAI_0D03810(NDAI0D03810) NDAI_0E03020(NDAI0E03020)

TPF:	TPHA_0F00500(TPHA0F00500) TPHA_0K01810(TPHA0K01810) TPHA_0L01370(TPHA0L01370)

TBL:	TBLA_0B00380(TBLA0B00380) TBLA_0C04290(TBLA0C04290) TBLA_0H03340(TBLA0H03340)

TGB:	HG536_0D04830 HG536_0H01410

TDL:	TDEL_0B01370(TDEL0B01370) TDEL_0F02760(TDEL0F02760)

KAF:	KAFR_0F03660(KAFR0F03660) KAFR_0H01390(KAFR0H01390)

KNG:	KNAG_0A02150(KNAG0A02150) KNAG_0A03990(KNAG0A03990) KNAG_0D03770(KNAG0D03770)

ZMK:	HG535_0B05480 HG535_0C01350

PPA:	PAS_chr1-3_0251 PAS_chr4_0103

DHA:	DEHA2C13508g DEHA2D15972g

PIC:	PICST_56149 PICST_76016(OCH1)

PGU:	PGUG_00592 PGUG_05622

SPAA:

SPAPADRAFT_143242 SPAPADRAFT_143660 SPAPADRAFT_143760 SPAPADRAFT_155280(OCH1) SPAPADRAFT_49565 SPAPADRAFT_52588 SPAPADRAFT_53501 SPAPADRAFT_59962 SPAPADRAFT_61486 SPAPADRAFT_62310 SPAPADRAFT_62316 SPAPADRAFT_62531 SPAPADRAFT_72461

LEL:	LELG_02734 LELG_02814

CAL:	CAALFM_C306020WA(MNN9) CAALFM_C306090CA(OCH1)

CTP:	CTRG_02254 CTRG_02261

COT:	CORT_0C02360 CORT_0C04350

CDU:	CD36_85950(MNN9) CD36_86020

CTEN:

CANTEDRAFT_129353 CANTEDRAFT_99095

YLI:	YALI0B12518g

CLU:	CLUG_01658 CLUG_02025

CLUS:

A9F13_02g02673 A9F13_13g01012

CAUR:

CJI97_002314 CJI97_002577

SLB:	AWJ20_134(HOC1) AWJ20_191(MNN9)

PKZ:	C5L36_0A08300 C5L36_0B02277 C5L36_0B07470 C5L36_0B08630 C5L36_0C02090

BNN:	FOA43_003835(MNN9) FOA43_004150

BBRX:

BRETT_000545 BRETT_002798 BRETT_003985

OPA:	HPODL_01382 HPODL_01489 HPODL_01625 HPODL_02407 HPODL_02408 HPODL_03209 HPODL_04510 HPODL_04840

SLUD:

SCDLUD_001544 SCDLUD_001970

NCR:	NCU00609(och-1) NCU02635

NTE:	NEUTE1DRAFT126859(NEUTE1DRAFT_126859) NEUTE1DRAFT91925(NEUTE1DRAFT_91925)

SMP:	SMAC_01297 SMAC_05727

PAN:	PODANSg3939 PODANSg5619

TTT:	THITE_2095073 THITE_2121506

MTM:	MYCTH_2106248 MYCTH_2297411 MYCTH_60177

CTHR:

CTHT_0014860 CTHT_0019180 CTHT_0057910

MGR:	MGG_00171 MGG_02859 MGG_05011 MGG_08652

PPEI:

PpBr36_03389 PpBr36_05778 PpBr36_08977 PpBr36_09260 PpBr36_10347 PpBr36_10775

TMN:	UCRPA7_2404 UCRPA7_8569

SSCK:

SPSK_09242 SPSK_09403

FGR:	FGSG_06856 FGSG_09425

FPU:	FPSE_07960 FPSE_11809

FVR:	FVEG_03975 FVEG_05131

FOX:	FOXG_01971 FOXG_06111

NHE:	NECHADRAFT_105919 NECHADRAFT_123326

TRE:	TRIREDRAFT_58609 TRIREDRAFT_65646 TRIREDRAFT_80340

TRR:	M419DRAFT_124397 M419DRAFT_131661 M419DRAFT_74592

MAW:	MAC_00995 MAC_06842 MAC_08481

MAJ:	MAA_05737 MAA_08458

CMT:	CCM_05217 CCM_06374

PLJ:	VFPFJ_05363 VFPFJ_06028

VAL:	VDBG_02349 VDBG_04673

VDA:	VDAG_02820 VDAG_04214

CFJ:	CFIO01_01535 CFIO01_03025

CLUP:

CLUP02_05583

CHIG:

CH63R_00695 CH63R_07086 CH63R_09786

SAPO:

SAPIO_CDS10094 SAPIO_CDS3303 SAPIO_CDS7989

ELA:	UCREL1_1692 UCREL1_7932 UCREL1_801 UCREL1_886

PFY:	PFICI_00433 PFICI_09035 PFICI_09794 PFICI_14506

SSL:	SS1G_02807 SS1G_03158

BFU:	BCIN_06g03220(Bcmnn9) BCIN_08g03990

MBE:	MBM_04797 MBM_07558

PSCO:

LY89DRAFT_313255 LY89DRAFT_594840 LY89DRAFT_676037 LY89DRAFT_699034

GLZ:	GLAREA_01840 GLAREA_05012 GLAREA_05067 GLAREA_10699

ANI:	AN4716.2 AN7672.2

AFM:	AFUA_2G01450 AFUA_5G08580

ACT:	ACLA_011950 ACLA_033030 ACLA_093460

NFI:	NFIA_033750 NFIA_078700

AOR:	AO090120000208 AO090701000814

ANG:	ANI_1_606064(An07g04940) ANI_1_624034(An03g05010)

AFV:	AFLA_005322 AFLA_009426

ALUC:

AKAW2_11724A(OCH1_1) AKAW2_50871A(MNN9)

ACHE:

ACHE_40559A(MNN9) ACHE_50551A(OCH1)

APUU:

APUU_71012S(OCH1) APUU_80223A(MNN9)

PCS:	Pc12g11090 Pc20g05550 Pc22g16540 Pc22g21550

PDP:	PDIP_07370 PDIP_51700 PDIP_63540

TMF:	PMAA_021680 PMAA_079830

TRG:	TRUGW13939_00569 TRUGW13939_03932 TRUGW13939_10718

CIM:	CIMG_00141 CIMG_06774

CPW:	CPC735_032020 CPC735_059970

URE:	UREG_00247 UREG_03690

PBL:	PAAG_03802 PAAG_04051 PAAG_05983

PBN:	PADG_03306 PADG_06012 PADG_07327

ABE:	ARB_05752 ARB_06643

TVE:	TRV_02425 TRV_04489

AJE:	HCAG_03327

BGH:	BDBG_01145 BDBG_06843

PNO:	SNOG_02920 SNOG_10597

PTE:	PTT_09202 PTT_11542 PTT_19588

BZE:	COCCADRAFT_10069 COCCADRAFT_26402 COCCADRAFT_41957 COCCADRAFT_6319 COCCADRAFT_95570

BSC:	COCSADRAFT_173170 COCSADRAFT_232790 COCSADRAFT_40014 COCSADRAFT_41481

BOR:	COCMIDRAFT_101902 COCMIDRAFT_34243 COCMIDRAFT_368 COCMIDRAFT_4455

AALT:

CC77DRAFT_1030960 CC77DRAFT_1052656 CC77DRAFT_361229

ZTR:	MYCGRDRAFT_103966 MYCGRDRAFT_33485 MYCGRDRAFT_70208

PFJ:	MYCFIDRAFT_152600 MYCFIDRAFT_27664

FFU:	CLAFUR5_01012 CLAFUR5_05986

BCOM:

BAUCODRAFT_285440 BAUCODRAFT_30856 BAUCODRAFT_75204

NPA:	UCRNP2_1719 UCRNP2_3813

TML:	GSTUM_00004829001 GSTUM_00011096001

SPO:	SPAC1006.05c(och1) SPAC4F10.10c(mnn9)

CNE:	CNA07230 CND03380 CNF01430

CNB:	CNBA7030 CNBD2980 CNBF3280

CNG:	CNAG_00744 CNAG_01214 CNAG_05836

CGI:	CGB_A8320C CGB_D6060C CGB_F4610W

TMS:	TREMEDRAFT_16005 TREMEDRAFT_58229 TREMEDRAFT_61657 TREMEDRAFT_70437

TASA:

A1Q1_01062 A1Q1_01267

UMA:	UMAG_02624

PFP:	PFL1_01943

MLR:	MELLADRAFT_27594

SMIN:

v1.2.008337.t1(symbB.v1.2.008337.t1) v1.2.008337.t2(symbB.v1.2.008337.t2) v1.2.009943.t1(symbB.v1.2.009943.t1) v1.2.035750.t1(symbB.v1.2.035750.t1)

TPS:	THAPS_264481

AAF:	AURANDRAFT_63262 AURANDRAFT_66517

SNG:	SNE_A00360

» show all

Reference

1 [PMID:2644248]

Authors

Romero PA, Herscovics A.

Title

Glycoprotein biosynthesis in Saccharomyces cerevisiae. Characterization of alpha-1,6-mannosyltransferase which initiates outer chain formation.

Journal

J Biol Chem 264:1946-50 (1989)

Reference

2 [PMID:1820199]

Authors

Reason AJ, Dell A, Romero PA, Herscovics A.

Title

Specificity of the mannosyltransferase which initiates outer chain formation in Saccharomyces cerevisiae.

Journal

Glycobiology 1:387-91 (1991)
DOI:10.1093/glycob/1.4.387

Reference

3 [PMID:8253757]

Authors

Nakanishi-Shindo Y, Nakayama K, Tanaka A, Toda Y, Jigami Y.

Title

Structure of the N-linked oligosaccharides that show the complete loss of alpha-1,6-polymannose outer chain from och1, och1 mnn1, and och1 mnn1 alg3 mutants of Saccharomyces cerevisiae.

Journal

J Biol Chem 268:26338-45 (1993)

Sequence

[sce:YGL038C]

Reference

4 [PMID:12784644]

Authors

Yamamoto K, Okamoto M, Yoko-o T, Jigami Y.

Title

Salt stress induces the expression of Schizosaccharomyces pombe och1+, which encodes an initiation-specific alpha-1,6-mannosyltransferase for N-linked outer chain synthesis of cell wall mannoproteins.

Journal

Biosci Biotechnol Biochem 67:927-9 (2003)
DOI:10.1271/bbb.67.927

Sequence

[spo:SPAC1006.05c]

Reference

5 [PMID:11754484]

Authors

Cui Z, Horecka J, Jigami Y.

Title

Cdc4 is involved in the transcriptional control of OCH1, a gene encoding alpha-1,6-mannosyltransferase in Saccharomyces cerevisiae.

Journal

Yeast 19:69-77 (2002)
DOI:10.1002/yea.801

Reference

Authors

Tsukahara, K., Watanabe, T., Yoko-o, T. and Chigami, Y.

Title

Schizosaccharomyces pombe och1+ gene encoding alpha-1,6-mannosyltransferase and use of och1+ gene knockout fission yeast for production of glycoproteins with reduced glycosylation.

Journal

Jpn Kokai Tokkyo Koho Koho:11 (2001)

Reference

7 [PMID:9276464]

Authors

Nakayama K, Nakanishi-Shindo Y, Tanaka A, Haga-Toda Y, Jigami Y.

Title

Substrate specificity of alpha-1,6-mannosyltransferase that initiates N-linked mannose outer chain elongation in Saccharomyces cerevisiae.

Journal

FEBS Lett 412:547-50 (1997)
DOI:10.1016/S0014-5793(97)00634-0

Reference

8 [PMID:8797833]

Authors

Suzuki A, Shibata N, Suzuki M, Saitoh F, Takata Y, Oshie A, Oyamada H, Kobayashi H, Suzuki S, Okawa Y.

Title

Characterization of alpha-1,6-mannosyltransferase responsible for the synthesis of branched side chains in Candida albicans mannan.

Journal

Eur J Biochem 240:37-44 (1996)
DOI:10.1111/j.1432-1033.1996.0037h.x

Reference

9 [PMID:8146181]

Authors

Yip CL, Welch SK, Klebl F, Gilbert T, Seidel P, Grant FJ, O'Hara PJ, MacKay VL

Title

Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins.

Journal

Proc Natl Acad Sci U S A 91:2723-7 (1994)
DOI:10.1073/pnas.91.7.2723

Sequence

[sce:YPL050C]

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.232

IUBMB Enzyme Nomenclature:

2.4.1.232

ExPASy - ENZYME nomenclature database:

2.4.1.232

BRENDA, the Enzyme Database:

2.4.1.232

CAS:	346003-17-6

DBGET integrated database retrieval system