KEGG   ENZYME: 2.4.1.245
Entry
EC 2.4.1.245                Enzyme                                 
Name
alpha,alpha-trehalose synthase;
trehalose synthase;
trehalose synthetase;
UDP-glucose:glucose 1-glucosyltransferase;
TreT;
PhGT;
ADP-glucose:D-glucose 1-alpha-D-glucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
NDP-alpha-D-glucose:D-glucose 1-alpha-D-glucosyltransferase
Reaction(IUBMB)
NDP-alpha-D-glucose + D-glucose = alpha,alpha-trehalose + NDP [RN:R10525]
Reaction(KEGG)
R10525 > R08946 R11306
Substrate
NDP-alpha-D-glucose [CPD:C15541];
D-glucose [CPD:C00031]
Product
alpha,alpha-trehalose [CPD:C01083];
NDP [CPD:C00454]
Comment
Requires Mg2+ for maximal activity [1]. The enzyme-catalysed reaction is reversible [1]. In the reverse direction to that shown above, the enzyme is specific for alpha,alpha-trehalose as substrate, as it cannot use alpha- or beta-paranitrophenyl glucosides, maltose, sucrose, lactose or cellobiose [1]. While the enzymes from the thermophilic bacterium Rubrobacter xylanophilus and the hyperthermophilic archaeon Pyrococcus horikoshii can use ADP-, UDP- and GDP-alpha-D-glucose to the same extent [2,3], that from the hyperthermophilic archaeon Thermococcus litoralis has a marked preference for ADP-alpha-D-glucose [1] and that from the hyperthermophilic archaeon Thermoproteus tenax has a marked preference for UDP-alpha-D-glucose [4].
History
EC 2.4.1.245 created 2008, modified 2013
Pathway
ec00500  Starch and sucrose metabolism
ec01100  Metabolic pathways
Orthology
K13057  trehalose synthase
Genes
MSQBKP64_04260
MMAIsS8_4740
NHLNhal_1697 Nhal_3100
TEETel_12660
GBIPG2T_08535
SLIMSCL_1383
GEBGM18_3944
GEOGeob_2843(treT)
PCAPcar_3111(treT)
PACEA6070_00930
PEFA7E78_09185
DVEDESUT3_01350
DFLDFE_1562
PBIZLWC08_00945
DRTDret_0427
DAKDaAHT2_0474
DPRDespr_1889
AFWAnae109_3637
MXAMXAN_0781
MSDMYSTI_00757
MYMA176_006221
MFBMFUL124B02_37200
CCXCOCOR_06731(treT) COCOR_07114(treT1)
MFULILAB_00130 LILAB_04900
MMASMYMAC_000794 MYMAC_001828
AGEAA314_01726
AVMJQX13_08250 JQX13_21010
VINAKJ08_0183 AKJ08_2261
SAMYDB32_003048
LLUAKJ09_03277 AKJ09_03923
HOHHoch_4094
SATSYN_02626
DAODesac_0491 Desac_2074
DTIDesti_0427 Desti_1352
SFUSfum_2348
DAXFDQ92_12895
DAVDESACE_02520
DHRLGS26_06250
SAMESAMCFNEI73_pB0408(treT)
NGLRG1141_PA10020
BICLMTR13_32505
BVVBHK69_15155
MCGGL4_1007
NPPPP1Y_AT23076
AFRAFE_2083
AFELferr_1740
CALKHUE98_02235
PTHPTH_1589(RfaG)
DKUDesku_2761
TJRTherJR_2672
DGIDesgi_1716
HCVFTV88_2431
MTHOMOTHE_c18340(treT)
MTHZMOTHA_c19170(treT)
TPZTph_c26450(treT)
LPILLIP_3316
SALSSLNWT_0587
SCXAS200_14825
SFKKY5_0700
SCYAEJ357_02060
SASTCD934_05035
SKACP970_39495
STUIGCM10017668_68950
GYUFE374_16990
NOYEXE57_05525
NSNEXE58_03690
NMESH9L09_04205
NPIG7071_15030
AEZC3E78_08730
LEDBBK82_03990
ACTIUA75_21495
ACADUA74_21015
MCABHXZ27_10420
AMSAMIS_32200
ACTNL083_2862
PFLAPflav_016310
ACQAM609_01085
AOSAXE84_03145
ARDAXF14_06245
ACTPB6G06_08385
ACTCCHIBA101_0362
ACTOC3V41_11440
ANEAN27039_0562
AHWNCTC11636_01420(treT)
ACTZCWT12_01250
AIRNCTC12972_01655(treT)
ASLANCTC11923_00301(treT)
AVCNCTC10951_01814(treT)
AWEJG540_01455
AMAMHPC72_01260
AREPID810_10725
RXYRxyl_2973 Rxyl_3082
RUBGBA63_16795 GBA63_18845
BSOLFSW04_00020 FSW04_25355
CWOCwoe_5908
HHGXM38_036220
TROtrd_0944
STISthe_2106
KBSEPA93_23110
THUGKNN16_10145
TTRTter_1718
PUVPUV_09960
TTFTHTE_2039
BROCIPI25_10760
BPITBPIT_27320
JETL3J17_03195 L3J17_15505
VAIBU251_03530
EMIEmin_1550
TAITaci_0297
ACOAmico_0248
AMOAnamo_1584
RBIRB2501_12587
MRSMurru_0499
MUTGVT53_12745
MAQILDL77_04175
HTHHTH_0760
HTEHydth_0761
TTKTST_0833(treT)
TMATM0392
TMMTmari_0389
TMITHEMA_02770
TMWTHMA_0397
TMQTHMB_0397
TMXTHMC_0397
TPTTpet_0528
TRQTRQ2_0542
TNACTN_0277
TNPTnap_0184
THQT2812B_02775
THZCELL2_02785
THRTRQ7_02890
TLETlet_1841
TTATheth_1807
PHYAJ81_09270
TMETmel_1821
TAFTHA_843
THPBG95_00665
THERY592_00670
TFEJYK00_08650
FNOFnod_0451
FPEFerpe_0502
FIANA23_02830
KOLKole_0299
KPFIX53_10025
MPGTheba_0438
MINFMESINF_2196(treT)
CPOCOPRO5265_1552
CEXCSE_06690
DTPJZK55_03590
NDENIDE3904
NMVNITMOv2_3873(treT)
NJANSJP_2226(treT)
NKFNkreftii_002979 Nkreftii_003019
TIDThein_0594
TOPTOPB45_0482
TCMHL41_04365
THETF1847_04305
TAVG4V39_05715
TMAIFVE67_03530
ALAMRT761_01717(treT)
BANABARAN1_0519(treT)
BIHBIP78_0183 BIP78_0300
PFUPF1742
PFIPFC_09885
PHOPH1035(PH1035)
PYSPy04_1331
PYCTQ32_10875
TSITSIB_0361
TBATERMP_01971
TLTOCC_03547
THSTES1_1927
TEUTEU_03525
TGYX802_07250
TGGA3K92_06315
TBSA3L01_06740
THHCDI07_06705
TTDA3L14_04435
TPRFA3L09_09045
TRLA3L10_03700
PPACPAP_05120
MTPMthe_0797
MEMMemar_1192 Memar_1763 Memar_2023
MBGBN140_0244 BN140_0628 BN140_1261
MEMAMMAB1_0304(treT) MMAB1_0814(treT) MMAB1_1593(treT)
MCHKMchiMG62_13560 MchiMG62_20220 MchiMG62_23340
HDSHSR122_0047(rfaG)
NMGNmag_3832
HXAHalxa_0121
NGENatgr_2896
HRUHalru_0712 Halru_2965
NOUNatoc_3903
HLCCHINAEXTREME19245
NAJB1756_05760
NAGAArcMg_1074
NANAArc1_2549
NBGDV706_17415
NASGCU68_11465
SMRSmar_0085
SHCShell_0727
STOSTK_04040
SOHD1869_01750
SSOSSO2609
SOLSsol_0419
SSOASULA_0413
SSOLSULB_0415
SSOFSULC_0413
SSHIJ5U23_00884
SCASSACC_22730
SAISaci_1827
SACNSacN8_08915
SACRSacRon12I_08925
SACSSUSAZ_08770
SISLS215_2870
SIAM1425_2703
SIMM1627_2757
SIDM164_2688
SIYYG5714_2882
SINYN1551_3067
SIILD85_3027
SIHSiH_2660
SIRSiRe_2596
SICSiL_2546
SULABFU36_09315
SULEGFS03_01030
SULOGFS33_06230
SULLEWF20_03220
MSEMsed_0129
MCNMcup_1929
MHKDFR87_06280
MPRUDFR88_06010
MTENGWK48_06110
MJNMjAS7_0726
AHOAhos_0030
AMANB6F84_02180
ABRIDFR85_01780
ASULDFR86_02160
AAMBD1866_07015
ACIHHS5_12860
SACDHS1genome_0766
SAZOD1868_02385
CSTYKN1_19670
STEPIC006_2529
SAHSHS7_05280
PAIPAE1278
PCLPcal_1359
PASPars_0594
PYRP186_2621
POGPogu_1761
PYWPYWP30_00458
TTNTTX_0217(treT)
TUZTUZN_0976
TPETpen_1592
THFMA03_01635
THELIG193_01540
 » show all
Reference
1  [PMID:15364950]
  Authors
Qu Q, Lee SJ, Boos W.
  Title
TreT, a novel trehalose glycosyltransferring synthase of the hyperthermophilic archaeon Thermococcus litoralis.
  Journal
J Biol Chem 279:47890-7 (2004)
DOI:10.1074/jbc.M404955200
  Sequence
[tlt:OCC_03547]
Reference
2  [PMID:15737605]
  Authors
Ryu SI, Park CS, Cha J, Woo EJ, Lee SB
  Title
A novel trehalose-synthesizing glycosyltransferase from Pyrococcus horikoshii: molecular cloning and characterization.
  Journal
Biochem Biophys Res Commun 329:429-36 (2005)
DOI:10.1016/j.bbrc.2005.01.149
  Sequence
[pho:PH1035]
Reference
3  [PMID:18835983]
  Authors
Nobre A, Alarico S, Fernandes C, Empadinhas N, da Costa MS
  Title
A unique combination of genetic systems for the synthesis of trehalose in Rubrobacter xylanophilus: properties of a rare actinobacterial TreT.
  Journal
J Bacteriol 190:7939-46 (2008)
DOI:10.1128/JB.01055-08
  Sequence
[rxy:Rxyl_2973]
Reference
4  [PMID:18483808]
  Authors
Kouril T, Zaparty M, Marrero J, Brinkmann H, Siebers B
  Title
A novel trehalose synthesizing pathway in the hyperthermophilic Crenarchaeon Thermoproteus tenax: the unidirectional TreT pathway.
  Journal
Arch Microbiol 190:355-69 (2008)
DOI:10.1007/s00203-008-0377-3
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.245
IUBMB Enzyme Nomenclature: 2.4.1.245
ExPASy - ENZYME nomenclature database: 2.4.1.245
BRENDA, the Enzyme Database: 2.4.1.245

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