KEGG ENZYME: 2.4.1.254

ENZYME: 2.4.1.254

Entry

EC 2.4.1.254                Enzyme

Name

cyanidin-3-O-glucoside 2''-O-glucuronosyltransferase;
BpUGT94B1;
UDP-glucuronic acid:anthocyanin glucuronosyltransferase;
UDP-glucuronic acid:anthocyanidin 3-glucoside 2'-O-beta-glucuronosyltransferase;
BpUGAT;
UDP-D-glucuronate:cyanidin-3-O-beta-glucoside 2-O-beta-glucuronosyltransferase

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-alpha-D-glucuronate:cyanidin-3-O-beta-D-glucoside 2-O-beta-D-glucuronosyltransferase

Reaction(IUBMB)

UDP-alpha-D-glucuronate + cyanidin 3-O-beta-D-glucoside = UDP + cyanidin 3-O-(2-O-beta-D-glucuronosyl)-beta-D-glucoside [RN:R09635]

Reaction(KEGG)

R09635;
(other) R07932

Substrate

UDP-alpha-D-glucuronate [CPD:C00167];
cyanidin 3-O-beta-D-glucoside [CPD:C08604]

Product

UDP [CPD:C00015];
cyanidin 3-O-(2-O-beta-D-glucuronosyl)-beta-D-glucoside [CPD:C19762]

Comment

The enzyme is highly specific for cyanidin 3-O-glucosides and UDP-alpha-D-glucuronate. Involved in the production of glucuronosylated anthocyanins that are the origin of the red coloration of flowers of Bellis perennis [1].

History

EC 2.4.1.254 created 2011

Pathway

ec00942

Anthocyanin biosynthesis

Orthology

K12937

cyanidin-3-O-glucoside 2''-O-glucuronosyltransferase

Reference

1 [PMID:15509561]

Authors

Sawada S, Suzuki H, Ichimaida F, Yamaguchi MA, Iwashita T, Fukui Y, Hemmi H, Nishino T, Nakayama T.

Title

UDP-glucuronic acid:anthocyanin glucuronosyltransferase from red daisy (Bellis perennis) flowers. Enzymology and phylogenetics of a novel glucuronosyltransferase involved in flower pigment biosynthesis.

Journal

J Biol Chem 280:899-906 (2005)
DOI:10.1074/jbc.M410537200

Sequence

[ag:BAD77944]

Reference

2 [PMID:18829982]

Authors

Osmani SA, Bak S, Imberty A, Olsen CE, Moller BL

Title

Catalytic key amino acids and UDP-sugar donor specificity of a plant glucuronosyltransferase, UGT94B1: molecular modeling substantiated by site-specific mutagenesis and biochemical analyses.

Journal

Plant Physiol 148:1295-308 (2008)
DOI:10.1104/pp.108.128256

Sequence

[ag:BAD77944]

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.254

IUBMB Enzyme Nomenclature:

2.4.1.254

ExPASy - ENZYME nomenclature database:

2.4.1.254

BRENDA, the Enzyme Database:

2.4.1.254

All links

Pathway (2)   
   KEGG PATHWAY (2)   
Chemical substance (6)   
   KEGG COMPOUND (6)   
Chemical reaction (4)   
   KEGG REACTION (2)   
   KEGG RCLASS (2)   
Gene (2)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (1)   
Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
Protein domain (2)   
   InterPro (2)   
All databases (18)   

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