KEGG   ENZYME: 2.4.1.256
Entry
EC 2.4.1.256                Enzyme                                 

Name
dolichyl-P-Glc:Glc2Man9GlcNAc2-PP-dolichol alpha-1,2-glucosyltransferase;
ALG10;
Dol-P-Glc:Glc2Man9GlcNAc2-PP-Dol alpha-1,2-glucosyltransferase;
dolichyl beta-D-glucosyl phosphate:D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol 2-alpha-D-glucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
dolichyl beta-D-glucosyl-phosphate:alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol alpha-1,2-glucosyltransferase (configuration-retaining)
Reaction(IUBMB)
dolichyl beta-D-glucosyl phosphate + alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol = dolichyl phosphate + alpha-D-Glc-(1->2)-alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol [RN:R06264]
Reaction(KEGG)
R06264(G)
Substrate
dolichyl beta-D-glucosyl phosphate [CPD:C01246];
alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
Product
dolichyl phosphate [CPD:C00110];
alpha-D-Glc-(1->2)-alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
Comment
This eukaryotic enzyme performs the final step in the synthesis of the lipid-linked oligosaccharide, attaching D-glucose in an alpha-1,2-linkage to the outermost D-glucose in the long branch. The lipid-linked oligosaccharide is involved in N-linked protein glycosylation of selected asparagine residues of nascent polypeptide chains in eukaryotic cells.
History
EC 2.4.1.256 created 2011, modified 2012
Pathway
ec00510  N-Glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K03850  alpha-1,2-glucosyltransferase
Genes
HSA: 144245(ALG10B) 84920(ALG10)
PTR: 451831 735940(ALG10)
PPS: 100971669(ALG10)
GGO: 101124291(ALG10B) 115930196
PON: 100459566(ALG10) 100462149
NLE: 100581890 100591198
MCC: 696470
MCF: 102129056(ALG10)
CSAB: 103218846
CATY: 105591351
PANU: 101022253
RRO: 104680792(ALG10)
RBB: 108519010
TFN: 117090304(ALG10)
PTEH: 111540579
CJC: 100414177(ALG10)
SBQ: 101043301(ALG10)
MMUR: 105881001
MMU: 380959(Alg10b)
MCAL: 110310698(Alg10)
MPAH: 110334834(Alg10)
RNO: 245960(Alg10)
MCOC: 116078139
MUN: 110553681
CGE: 100753866
PLEU: 114693038
NGI: 103731291(Alg10)
HGL: 101710247(Alg10)
CCAN: 109698292
OCU: 100338055(ALG10)
TUP: 102499471(ALG10)
CFA: 477647
VVP: 112921049
VLG: 121479691
AML: 100480278
UMR: 103675386(ALG10)
UAH: 113257737
ORO: 101366944(ALG10)
ELK: 111161353
MPUF: 101680548
EJU: 114216559
MLX: 118025515
FCA: 101092445(ALG10)
PYU: 121038735
PTG: 102958960(ALG10)
PPAD: 109268819(ALG10)
AJU: 106977425
HHV: 120226246
BTA: 539837(ALG10)
BOM: 102275661(ALG10)
BIU: 109559540
BBUB: 102406990
CHX: 102179173(ALG10)
OAS: 101104224
CCAD: 122436496
SSC: 100525643(ALG10)
CFR: 102517383(ALG10)
CBAI: 105068267(ALG10)
CDK: 105090579(ALG10)
BACU: 103014173(ALG10)
LVE: 103070115(ALG10)
OOR: 101285322(ALG10)
DLE: 111176903
PCAD: 102993649(ALG10)
ECB: 100064952
EPZ: 103561030
EAI: 106833444
MYB: 102241433(ALG10)
MYD: 102764376(ALG10)
MMYO: 118650960
MNA: 107528375(ALG10)
HAI: 109374405
DRO: 112318398
SHON: 118995213
AJM: 119045097
MMF: 118624357
PALE: 102879937
PGIG: 120618281
RAY: 107498428
MJV: 108398423
TOD: 119247118
TMU: 101348246
MDO: 100023650(ALG10)
SHR: 100934370
PCW: 110199044(ALG10)
OAA: 100073466
GGA: 426734(ALG10)
PCOC: 116234298
MGP: 100550477
CJO: 107308112
NMEL: 110392405
LSR: 110467627
SCAN: 103813504
PMOA: 120510659
GFR: 102034176(ALG10B)
FAB: 101806662(ALG10B)
PHI: 102106098(ALG10B)
PMAJ: 107204775(ALG10)
CCAE: 111923022(ALG10)
CCW: 104686912
ETL: 114060537
FCH: 102052568
CLV: 102097373
EGZ: 104123800(ALG10)
NNI: 104010788(ALG10)
ACUN: 113491353
PADL: 103917275
AAM: 106487727(ALG10B)
NPD: 112953316
ASN: 102373115
AMJ: 102575178(ALG10B)
CPOO: 109315700
GGN: 109292115(ALG10B)
PSS: 102460425
CMY: 102934644
CPIC: 101950653
TST: 117875565
CABI: 116820936
ACS: 100563081(alg10)
PVT: 110080795
PBI: 103065196
TSR: 106550290
PGUT: 117679820
PMUA: 114605086
ZVI: 118090609
GJA: 107113945
XLA: 108711169(alg10.L) 432047(alg10.S)
XTR: 548946(alg10)
NPR: 108783835(ALG10)
DRE: 100331575(alg10)
IPU: 108279920(alg10)
PHYP: 113532360
AMEX: 103027857(alg10)
EEE: 113580192
TRU: 101071925(alg10)
LCO: 104938651
NCC: 104959434
CGOB: 115028281(alg10)
ELY: 117249306(alg10)
PLEP: 121961269(alg10)
SLUC: 116039778(alg10)
ECRA: 117938415(alg10)
PFLV: 114549935(alg10)
GAT: 120817839(alg10)
MSAM: 119918097(alg10)
CUD: 121505231(alg10)
MZE: 101470227
ONL: 100692975
OAU: 116334943(alg10)
OLA: 101157993(alg10)
OML: 112157106(alg10)
XMA: 102218101(alg10)
XCO: 114147003
XHE: 116736884
PRET: 103459531(alg10)
CVG: 107099795
CTUL: 119776243(alg10)
NFU: 107383719
KMR: 108244056(alg10)
ALIM: 106524852
AOCE: 111582869
CSEM: 103382932(alg10)
POV: 109632917
LCF: 108899835(alg10)
SDU: 111224975
SLAL: 111667278
XGL: 120799478(alg10)
HCQ: 109520135(alg10)
MALB: 109960941(alg10)
OMY: 110490168(alg10) 110500280
SNH: 120031965(alg10) 120049317
ELS: 105028210(alg10)
SFM: 108920848
PKI: 111843423
AANG: 118232650(alg10)
LOC: 102683387(alg10)
ARUT: 117415264(alg10) 117419566
LCM: 102346649(ALG10B)
CMK: 103188282
RTP: 109917198
BBEL: 109477306
CIN: 100184563
SCLV: 120339581
SPU: 590617
APLC: 110974016
DME: Dmel_CG32076(Alg10)
DER: 6543808
DSE: 6605240
DSI: Dsimw501_GD14291(Dsim_GD14291)
DAN: 6507873
DSR: 110190249
DPE: 6598973
DMN: 108151774
DWI: 6645864
DGR: 6558396
DAZ: 108612615
DNV: 108652310
DHE: 111604477
DVI: 6623132
CCAT: 101463099
MDE: 101892420
LCQ: 111686476
ACOZ: 120948993
AARA: 120896014
AAG: 5569660
CPII: 120414421
AME: 102655688
ACER: 107995976
BIM: 100744021
BBIF: 117214044
BTER: 100648794
CCAL: 108624102
OBB: 114877717
MGEN: 117229717
NMEA: 116434760
CGIG: 122397558
SOC: 105204467
MPHA: 105837462
AEC: 105154554
ACEP: 105621143
PBAR: 105428180
VEM: 105563132
HST: 105188845
DQU: 106744200
CFO: 105248857
FEX: 115238700
LHU: 105668917
PGC: 109854307
OBO: 105277645
PCF: 106786294
NVI: 103316515
CSOL: 105367866
MDL: 103568569
CCIN: 107269669
TCA: 661882
DPA: 109532867
ATD: 109598081
AGB: 108907485
NVL: 108567728
APLN: 108737554
PPYR: 116171116
OTU: 111425310
BMOR: 101745380
BMAN: 114246169
MSEX: 115450758
BANY: 112045717
PMAC: 106714301
PPOT: 106107541
PXU: 106115338
PRAP: 110993707
ZCE: 119839060
TNL: 113505579
API: 100573721
AGS: 114125421
RMD: 113557253
DCI: 103507241
CLEC: 106666658
HHAL: 106684209
NLU: 111059688
CSEC: 111872024
FCD: 110841871
DMK: 116920788
PVM: 113822748
HAME: 121869020
HAZT: 108672751
RSAN: 119394453
VDE: 111245947
VJA: 111271529
TUT: 107367937
DPTE: 113797750
CSCU: 111632263
PTEP: 107437241
SDM: 118190831
CEL: CELE_T24D1.4(algn-10)
CBR: CBG03825(Cbr-tag-179)
BMY: BM_BM5986(Bm5986)
PCAN: 112571593
BGT: 106052134
GAE: 121369480
CRG: 105332981
MYI: 110461259
PMAX: 117330508
OBI: 106876907
LAK: 106172617
EGL: EGR_06905
NVE: 5511131
EPA: 110243305
ATEN: 116296660
ADF: 107340959
AMIL: 114969054
PDAM: 113667013
SPIS: 111339426
DGT: 114521700
HMG: 100197595
AQU: 100637452
ATH: AT5G02410(ALG10)
ALY: 9307041
CRB: 17883736
BRP: 103855464
RSZ: 108844297
THJ: 104809903
CPAP: 110825892
CIT: 102613758
TCC: 18597765
GRA: 105799851
GAB: 108454810
DZI: 111295358
EGR: 104426472
VRA: 106754554
VAR: 108329529
VUN: 114183103
CCAJ: 109791189
APRC: 113863054
CAM: 101502941
LJA: Lj0g3v0182329.1(Lj0g3v0182329.1) Lj0g3v0182329.2(Lj0g3v0182329.2)
ADU: 107474865
AIP: 107625566
LANG: 109361297
FVE: 101293604
RCN: 112192458
PPER: 18772348
PMUM: 103339514
PAVI: 110761035
PDUL: 117631448
ZJU: 107430592
MNT: 21404159
CSV: 101214003
CMO: 103490227
BHJ: 120069892
MCHA: 111013674
CMAX: 111466254
CMOS: 111438157
CPEP: 111782301
RCU: 8258446
JCU: 105649562
HBR: 110631521
MESC: 110605349
POP: 18099975
PEU: 105122465
PALZ: 118053372
JRE: 108999020
TWL: 119988813
VVI: 100249516
VRI: 117921241
SLY: 101252805
SPEN: 107029414
SOT: 102605691
CANN: 107842996
NSY: 104223128
NTO: 104102446
NAU: 109237223
INI: 109159141
ITR: 116028526
SIND: 105174445
OEU: 111410869
EGT: 105952955
HAN: 110915476
LSV: 111887176
CCAV: 112522659
CSIN: 114297073
BVG: 104889508
SOE: 110775635
CQI: 110710652
NNU: 104592573
OSA: 4336325
DOSA: Os04t0503700-01(Os04g0503700)
OBR: 102720597
BDI: 100827541
ATS: 109781116
SBI: 110436308
ZMA: 100384825
SITA: 101753177
PHAI: 112898931
PDA: 103708230
EGU: 105040462
MUS: 103991712
DCT: 110105454
PEQ: 110028102
AOF: 109846602
ATR: 18437339
SMO: SELMODRAFT_96516(GT59A1)
PPP: 112280910
MNG: MNEG_0009
APRO: F751_1473
SCE: YGR227W(DIE2)
ERC: Ecym_2078
KMX: KLMA_60055(ALG10)
NCS: NCAS_0F00630(NCAS0F00630)
NDI: NDAI_0D02500(NDAI0D02500)
TPF: TPHA_0A05070(TPHA0A05070)
TBL: TBLA_0B07410(TBLA0B07410)
TDL: TDEL_0G01240(TDEL0G01240)
KAF: KAFR_0B04220(KAFR0B04220)
PIC: PICST_43096(DIE2)
SPAA: SPAPADRAFT_56279(ALG10)
CAL: CAALFM_C305340WA(DIE2)
NCR: NCU16845
NTE: NEUTE1DRAFT128956(NEUTE1DRAFT_128956)
MGR: MGG_03990
SSCK: SPSK_08598
MAW: MAC_03989
MAJ: MAA_07026
CMT: CCM_00015
BFU: BCIN_15g04220(Bcdie2)
MBE: MBM_06558
ANI: AN5902.2
ANG: ANI_1_404024(An02g02980)
CIM: CIMG_12056(CIMG07985)
ABE: ARB_01400
TVE: TRV_02220
PTE: PTT_02199
SPO: SPAC56F8.06c(alg10)
LBC: LACBIDRAFT_399396(ALG10)
ABP: AGABI1DRAFT50659(AGABI1DRAFT_50659)
MRT: MRET_0177
MSYM: MSY001_2039(DIE2)
DDI: DDB_G0277817(alg10)
TPV: TP01_1227
SMIN: v1.2.021974.t1(symbB.v1.2.021974.t1)
SPAR: SPRG_10004
 » show all
Reference
1  [PMID:9597543]
  Authors
Burda P, Aebi M
  Title
The ALG10 locus of Saccharomyces cerevisiae encodes the alpha-1,2 glucosyltransferase of the endoplasmic reticulum: the terminal glucose of the lipid-linked oligosaccharide is required for efficient N-linked glycosylation.
  Journal
Glycobiology 8:455-62 (1998)
DOI:10.1093/glycob/8.5.455
  Sequence
[sce:YGR227W]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.256
IUBMB Enzyme Nomenclature: 2.4.1.256
ExPASy - ENZYME nomenclature database: 2.4.1.256
BRENDA, the Enzyme Database: 2.4.1.256

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