KEGG   ENZYME: 2.4.1.258
Entry
EC 2.4.1.258                Enzyme                                 

Name
dolichyl-P-Man:Man5GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase;
Man5GlcNAc2-PP-Dol mannosyltransferase;
ALG3;
dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase;
Not56-like protein;
Alg3 alpha-1,3-mannosyl transferase;
Dol-P-Man:Man5GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase;
dolichyl beta-D-mannosyl phosphate:D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,3-mannosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
dolichyl beta-D-mannosyl-phosphate:alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol 3-alpha-D-mannosyltransferase (configuration-inverting)
Reaction(IUBMB)
dolichyl beta-D-mannosyl phosphate + alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol = alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol + dolichyl phosphate [RN:R06258]
Reaction(KEGG)
R06258(G)
Substrate
dolichyl beta-D-mannosyl phosphate [CPD:C03862];
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
Product
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol;
dolichyl phosphate [CPD:C00110]
Comment
The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl diphosphate. Early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-dolichol to Man9Glc-NAc2-PP-dolichol on the lumenal side use dolichyl beta-D-mannosyl phosphate. The first step of this assembly pathway on the luminal side of the endoplasmic reticulum is catalysed by ALG3.
History
EC 2.4.1.258 created 1976 as EC 2.4.1.130, part transferred 2011 to EC 2.4.1.258, modified 2012
Pathway
ec00510  N-Glycan biosynthesis
ec00513  Various types of N-glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K03845  alpha-1,3-mannosyltransferase
Genes
HSA: 10195(ALG3)
PTR: 460886(ALG3)
PPS: 100979287(ALG3)
GGO: 101148766(ALG3)
PON: 100448751(ALG3)
NLE: 100581760(ALG3)
MCC: 703087(ALG3)
MCF: 102134126(ALG3)
CSAB: 103221139(ALG3)
CATY: 105573975(ALG3)
RRO: 104664632(ALG3)
RBB: 108524997(ALG3)
TFN: 117083818(ALG3)
CJC: 100390805(ALG3)
SBQ: 101045776(ALG3)
MMUR: 105855769(ALG3)
MMU: 208624(Alg3)
MCAL: 110311374(Alg3)
MPAH: 110330167(Alg3)
RNO: 287983(Alg3)
MCOC: 116085725(Alg3)
MUN: 110549394(Alg3)
CGE: 100772003(Alg3)
PLEU: 114683317(Alg3)
NGI: 103732650(Alg3)
HGL: 101714764(Alg3)
CCAN: 109694096(Alg3)
OCU: 100358108(ALG3)
TUP: 102473222(ALG3)
CFA: 478653(ALG3)
VVP: 112926262(ALG3)
VLG: 121477489(ALG3)
AML: 100472334(ALG3)
UMR: 103676182(ALG3)
UAH: 113254352(ALG3)
ORO: 101377982(ALG3)
ELK: 111150611
MPUF: 101674865(ALG3)
EJU: 114207156(ALG3)
FCA: 101087681(ALG3)
PTG: 102971503(ALG3)
PPAD: 109275223(ALG3)
AJU: 106983810(ALG3)
HHV: 120238109(ALG3)
BTA: 614624(ALG3)
BOM: 102285313(ALG3)
BIU: 109557412(ALG3)
BBUB: 102402457(ALG3)
CHX: 102181899(ALG3)
OAS: 101121650(ALG3)
ODA: 120871977(ALG3)
SSC: 100627022(ALG3)
CFR: 102504086(ALG3)
CDK: 105085886(ALG3)
BACU: 103005524(ALG3)
LVE: 103084099(ALG3)
OOR: 101272032(ALG3)
DLE: 111173360(ALG3)
PCAD: 102996118(ALG3)
ECB: 100059007(ALG3)
EPZ: 103554746(ALG3)
EAI: 106834523(ALG3)
MYB: 102241054(ALG3)
MYD: 102766472(ALG3)
MMYO: 118676800(ALG3)
MNA: 107541086(ALG3)
HAI: 109396699(ALG3)
DRO: 112305128(ALG3)
SHON: 119004248(ALG3)
AJM: 119056434(ALG3)
MMF: 118617287(ALG3)
PALE: 102890134(ALG3)
RAY: 107504920(ALG3)
MJV: 108395266(ALG3)
LAV: 100674341(ALG3)
TMU: 101360342
MDO: 100025876(ALG3)
SHR: 100932630(ALG3)
PCW: 110221182(ALG3)
OAA: 100681362(ALG3)
GGA: 424952(ALG3)
PCOC: 116235396(ALG3)
MGP: 100543165(ALG3)
CJO: 107318207(ALG3)
NMEL: 110397801(ALG3)
APLA: 101791055(ALG3)
ACYG: 106032204(ALG3)
TGU: 100230731(ALG3)
LSR: 110477428(ALG3)
SCAN: 103815608(ALG3)
PMOA: 120504281(ALG3)
GFR: 102039749(ALG3)
FAB: 101819823(ALG3)
PHI: 102102878(ALG3)
PMAJ: 107208518(ALG3)
CCAE: 111933602(ALG3)
CCW: 104688139(ALG3)
ETL: 114060771(ALG3)
FPG: 101916756(ALG3)
FCH: 102050162(ALG3)
CLV: 102094667(ALG3)
EGZ: 104122229(ALG3)
NNI: 104015704(ALG3)
ACUN: 113483436(ALG3)
AAM: 106500262(ALG3)
ASN: 102369785(ALG3)
AMJ: 102560066(ALG3)
CPOO: 109308279(ALG3)
GGN: 109287758(ALG3)
PSS: 102457770(ALG3)
CMY: 102937300(ALG3)
CPIC: 101941423(ALG3)
TST: 117883346(ALG3)
CABI: 116838579(ALG3)
ACS: 100561775(alg3)
PVT: 110091600(ALG3)
PBI: 103048200(ALG3)
PMUR: 107294401(ALG3)
TSR: 106552359(ALG3)
PMUA: 114598066(ALG3)
ZVI: 118093587(ALG3)
GJA: 107123667(ALG3)
XLA: 100037243(alg3.S)
XTR: 100127746(alg3)
NPR: 108804700(ALG3)
DRE: 553725(alg3)
CCAR: 109063669(alg3)
IPU: 108280746(alg3)
PHYP: 113533332(alg3)
AMEX: 103037087(alg3)
EEE: 113568455(alg3)
TRU: 101070707(alg3)
LCO: 104935094(alg3)
NCC: 104957796(alg3)
ELY: 117272230(alg3)
PLEP: 121951113(alg3)
SLUC: 116037306(alg3)
GAT: 120815564(alg3)
MSAM: 119902871(alg3)
MZE: 101479101(alg3)
ONL: 100699348(alg3)
OAU: 116323406(alg3)
OLA: 101167399(alg3)
OML: 112147515(alg3)
XMA: 102230775(alg3)
XCO: 114147052(alg3)
PRET: 103479375(alg3)
CVG: 107101045(alg3)
NFU: 107384062(alg3)
KMR: 108239541(alg3)
ALIM: 106528976(alg3)
AOCE: 111582584(alg3)
CSEM: 103395988(alg3)
POV: 109645187(alg3)
LCF: 108883543(alg3)
SDU: 111229092(alg3)
SLAL: 111654479(alg3)
XGL: 120799092(alg3)
HCQ: 109524102(alg3)
BPEC: 110158566(alg3)
MALB: 109957667(alg3)
SASA: 106600580(alg3)
OMY: 110508350(alg3) 110530733
ELS: 105021055(alg3)
SFM: 108935289(alg3)
PKI: 111847388(alg3)
LOC: 102686367(alg3)
ARUT: 117417177(alg3) 117423052
LCM: 102361691(ALG3)
CMK: 103182887(alg3)
RTP: 109916296(alg3)
BFO: 118430771
CIN: 100176327
SCLV: 120337103
SPU: 576672
APLC: 110978953
SKO: 100370088
DME: Dmel_CG4084(Alg3)
DER: 6547893
DSE: 6615760
DSI: Dsimw501_GD25022(Dsim_GD25022)
DAN: 6494688
DSR: 110176926
DPE: 6593024
DMN: 108158679
DWI: 6652196
DAZ: 108616984
DNV: 108654695
DHE: 111599911
MDE: 101887633
LCQ: 111680079
ACOZ: 120949007
AARA: 120895199
AAG: 5574793
AALB: 109415735
CPII: 120418874
AME: 724399
BIM: 100740988
BTER: 100643648
CCAL: 113464335
OBB: 114873283
MGEN: 117230112
NMEA: 116430340
SOC: 105199092
MPHA: 105833095
AEC: 105152598
ACEP: 105617243
PBAR: 105433742
VEM: 105563964
HST: 105182205
DQU: 106748045
CFO: 105251420
FEX: 115239308
LHU: 105668510
PGC: 109859340
OBO: 105280429
PCF: 106784472
NVI: 100115752
CSOL: 105360913
MDL: 103575039
TCA: 655451
DPA: 109539990
ATD: 109600158
AGB: 108904073
NVL: 108558978
APLN: 108743272
PPYR: 116161218
OTU: 111418690
BMOR: 101741427
BMAN: 114241636
MSEX: 115445089
PMAC: 106712323
PPOT: 106111036
PXU: 106116985
PRAP: 111002964
ZCE: 119830376
HAW: 110384151
TNL: 113492747
API: 103308067
DNX: 107172979
AGS: 114129907
RMD: 113554562
BTAB: 109033224
DCI: 103504828
CLEC: 106663453
NLU: 111052776
ZNE: 110833535
CSEC: 111864641
FCD: 110846318
PVM: 113822625
EAF: 111696599
DSV: 119446845
RSAN: 119389332
RMP: 119184181
VDE: 111253320
VJA: 111271896
DPTE: 113791018
CSCU: 111622730
PTEP: 107456683
SDM: 118181742
CEL: CELE_K09E4.2(algn-3)
CBR: CBG20997
TSP: Tsp_08712
PCAN: 112557649
BGT: 106078344
GAE: 121390238
CRG: 105346658
PMAX: 117345390
OBI: 106874294
LAK: 106174922
NVE: 5518637
EPA: 110251455
ATEN: 116299235
ADF: 107332168
AMIL: 114965518
PDAM: 113686199
SPIS: 111328572
DGT: 114518083
HMG: 100214877
ATH: AT2G47760(ALG3)
ALY: 9318197
CRB: 17890265
BRP: 103866347
BOE: 106337396
RSZ: 108813443
THJ: 104802172
CPAP: 110821460
CIT: 102617688
PVY: 116139023
TCC: 18613533
GRA: 105762674
DZI: 111314136
EGR: 104456792
VRA: 106758012
VAR: 108329708
VUN: 114186261
CCAJ: 109812263
APRC: 113862765
CAM: 101509815
LJA: Lj1g3v4899050.1(Lj1g3v4899050.1)
ADU: 107492492
AIP: 107645621
FVE: 101310494
RCN: 112172534
PPER: 18767523
PMUM: 103333910
PAVI: 110761861
PDUL: 117637036
ZJU: 107426367
MNT: 21392434
CSV: 101211394
CMO: 103492832
BHJ: 120087178
MCHA: 111020823
CMAX: 111488806
CMOS: 111456562
CPEP: 111807027
RCU: 8271753
JCU: 105642496
HBR: 110660934
MESC: 110615603
POP: 7457948
PEU: 105132014
PALZ: 118041494
JRE: 109003856
QLO: 115972020
TWL: 119983571
VVI: 100253790
VRI: 117917250
SLY: 101251511
SPEN: 107029293
SOT: 102598887
CANN: 107864015
NSY: 104225504
NTO: 104099176
INI: 109160269
ITR: 116014836
SIND: 105165232
OEU: 111394355
EGT: 105954033
HAN: 110895982
LSV: 111917946
CCAV: 112513534
DCR: 108224117
CSIN: 114310377
BVG: 104906606
SOE: 110797544
NNU: 104610985
MING: 122064043
PSOM: 113357561
NCOL: 116254698
OSA: 9266078
OBR: 102705966
BDI: 100822122
ATS: 109779517
SBI: 8056549
ZMA: 100276684
SITA: 101768406
PHAI: 112893636
EGU: 105060271
MUS: 103981289
DCT: 110101639
PEQ: 110026944
AOF: 109841030
ATR: 18430288
SMO: SELMODRAFT_96090(GT58A1)
PPP: 112287567
APRO: F751_3769
SCE: YBL082C(ALG3)
ERC: Ecym_7147
KMX: KLMA_80379(ALG3)
NCS: NCAS_0E02510(NCAS0E02510)
NDI: NDAI_0E04080(NDAI0E04080)
TPF: TPHA_0K00760(TPHA0K00760)
TBL: TBLA_0D00510(TBLA0D00510)
TDL: TDEL_0C02670(TDEL0C02670)
KAF: KAFR_0L01850(KAFR0L01850)
PIC: PICST_58095(RHK1)
CAL: CAALFM_C604340WA(CaO19.1092)
NCR: NCU06552
NTE: NEUTE1DRAFT78420(NEUTE1DRAFT_78420)
MGR: MGG_08010
SSCK: SPSK_09197
MAW: MAC_01438
MAJ: MAA_07040
CMT: CCM_00021
BFU: BCIN_12g04720(Bcalg3)
MBE: MBM_00831
ANI: AN0104.2
ANG: ANI_1_1172164(An18g02360)
ABE: ARB_04378
TVE: TRV_03168
PNO: SNOG_16505(SNOG_16504)
PTE: PTT_10389
SPO: SPAC7D4.06c(alg3)
CNE: CNL04800
CNB: CNBI2040
TASA: A1Q1_00798
LBC: LACBIDRAFT_247511(ALG3)
SCM: SCHCODRAFT_51393(alg3)
ABP: AGABI1DRAFT118456(AGABI1DRAFT_118456)
ABV: AGABI2DRAFT200258(AGABI2DRAFT_200258)
MRT: MRET_3365
MSYM: MSY001_3019(ALG3)
DDI: DDB_G0268238(alg3)
DFA: DFA_10860(alg3)
FCY: FRACYDRAFT_180557(ALG3)
SPAR: SPRG_04773
EHX: EMIHUDRAFT_426313(ALG3-1) EMIHUDRAFT_521974(ALG3-2)
LMA: LMJF_36_2040(ALG3)
LIF: LINJ_36_2160(ALG3)
LPAN: LPMP_352120(ALG3)
 » show all
Reference
1  [PMID:11308030]
  Authors
Sharma CB, Knauer R, Lehle L
  Title
Biosynthesis of lipid-linked oligosaccharides in yeast: the ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.
  Journal
Biol Chem 382:321-8 (2001)
DOI:10.1515/BC.2001.039
  Sequence
[sce:YBL082C]
Reference
2  [PMID:10660594]
  Authors
Cipollo JF, Trimble RB
  Title
The accumulation of Man(6)GlcNAc(2)-PP-dolichol in the Saccharomyces cerevisiae Deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm  addition in downstream oligosaccharide-lipid and glycoprotein glycan processing.
  Journal
J Biol Chem 275:4267-77 (2000)
DOI:10.1074/jbc.275.6.4267
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.258
IUBMB Enzyme Nomenclature: 2.4.1.258
ExPASy - ENZYME nomenclature database: 2.4.1.258
BRENDA, the Enzyme Database: 2.4.1.258

DBGET integrated database retrieval system