KEGG   ENZYME: 2.4.1.259
Entry
EC 2.4.1.259                Enzyme                                 

Name
dolichyl-P-Man:Man6GlcNAc2-PP-dolichol alpha-1,2-mannosyltransferase;
ALG9;
ALG9 alpha1,2 mannosyltransferase;
dolichylphosphomannose-dependent ALG9 mannosyltransferase;
ALG9 mannosyltransferase;
Dol-P-Man:Man6GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase;
dolichyl beta-D-mannosyl phosphate:D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,2-mannosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
dolichyl beta-D-mannosyl-phosphate:alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol 2-alpha-D-mannosyltransferase (configuration-inverting)
Reaction(IUBMB)
dolichyl beta-D-mannosyl phosphate + alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol = alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol + dolichyl phosphate [RN:R06259]
Reaction(KEGG)
R06259(G)
Substrate
dolichyl beta-D-mannosyl phosphate [CPD:C03862];
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
Product
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol;
dolichyl phosphate [CPD:C00110]
Comment
The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl diphosphate. Early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9Glc-NAc2-PP-Dol on the lumenal side use dolichyl beta-D-mannosyl phosphate. ALG9 mannosyltransferase catalyses the addition of two different alpha-1,2-mannose residues - the addition of alpha-1,2-mannose to Man6GlcNAc2-PP-Dol (EC 2.4.1.259) and the addition of alpha-1,2-mannose to Man8GlcNAc2-PP-Dol (EC 2.4.1.261).
History
EC 2.4.1.259 created 1976 as EC 2.4.1.130, part transferred 2011 to EC 2.4.1.259, modified 2012
Pathway
ec00510  N-Glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K03846  alpha-1,2-mannosyltransferase
Genes
HSA: 79796(ALG9)
PTR: 100614079(ALG9)
PPS: 100995778(ALG9)
GGO: 101133937(ALG9)
PON: 100460794(ALG9)
NLE: 100607044(ALG9)
MCC: 710567(ALG9)
MCF: 102121797(ALG9)
CSAB: 103248445(ALG9)
CATY: 105588702(ALG9)
PANU: 101005216(ALG9)
RRO: 104677341(ALG9)
RBB: 108536463
PTEH: 111540124(ALG9)
CJC: 100409047(ALG9)
SBQ: 101031322(ALG9)
MMUR: 105862925(ALG9)
MMU: 102580(Alg9)
MCAL: 110301973(Alg9)
MPAH: 110327746(Alg9)
RNO: 367083(Alg9)
MCOC: 116073881(Alg9)
MUN: 110562004(Alg9)
CGE: 100755062(Alg9)
PLEU: 114690256(Alg9)
NGI: 103733888(Alg9)
HGL: 101720220(Alg9)
CCAN: 109687768(Alg9)
OCU: 100355011(ALG9)
TUP: 102479989(FDXACB1)
CFA: 609002(ALG9)
VVP: 112925974(ALG9)
VLG: 121499866(ALG9)
AML: 100481101(ALG9)
UMR: 103662400(ALG9)
UAH: 113260062(ALG9)
ORO: 101363962(ALG9)
ELK: 111153921
MPUF: 101673932(ALG9)
EJU: 114211339(ALG9)
MLX: 118013221(ALG9)
FCA: 101092139(ALG9)
PYU: 121035374(ALG9)
PPAD: 109278665(ALG9)
AJU: 106984106(ALG9)
HHV: 120229891(ALG9)
BTA: 504346(ALG9)
BOM: 102284904(ALG9)
BIU: 109568790(ALG9)
BBUB: 102408785(ALG9)
CHX: 102170340(ALG9)
OAS: 101109477(ALG9)
ODA: 120876231(ALG9)
CCAD: 122449691(ALG9)
SSC: 100519965(ALG9)
CFR: 102505314(ALG9)
CBAI: 105080045(ALG9)
CDK: 105094226(ALG9)
BACU: 103018179(ALG9)
LVE: 103068287(ALG9)
OOR: 101275267(ALG9)
DLE: 111168037(ALG9)
PCAD: 102993135(ALG9)
ECB: 100070423(ALG9)
EPZ: 103555599(ALG9)
EAI: 106840207(ALG9)
MYB: 102254327(ALG9)
MYD: 102766625(ALG9)
MMYO: 118664331(ALG9)
MNA: 107531300(ALG9)
HAI: 109388712(ALG9)
DRO: 112314354(ALG9)
SHON: 119000518(ALG9)
AJM: 119042444(ALG9)
MMF: 118628217(ALG9)
PALE: 102888331(ALG9)
PGIG: 120621985(ALG9)
MJV: 108409554(ALG9)
TOD: 119240661(ALG9)
LAV: 100663818(ALG9)
TMU: 101345135
MDO: 100026504(ALG9)
PCW: 110210691(ALG9)
OAA: 100088414(ALG9)
PCOC: 116233219(ALG9)
MGP: 100550107(ALG9)
CJO: 107324267(ALG9)
NMEL: 110387622(ALG9)
APLA: 101804237(ALG9)
ACYG: 106039803(ALG9)
TGU: 100232104(ALG9)
LSR: 110470400(ALG9)
SCAN: 103823030(ALG9)
PMOA: 120508444(ALG9)
GFR: 102031408(ALG9)
FAB: 101816673(ALG9)
PHI: 102114628(ALG9)
PMAJ: 107214310(ALG9)
CCAE: 111939291(ALG9)
CCW: 104692086(ALG9)
ETL: 114067828(ALG9)
FPG: 101924837(ALG9)
FCH: 102053470(ALG9)
CLV: 102097454(ALG9)
EGZ: 104125940(ALG9)
NNI: 104008772(ALG9)
ACUN: 113488541(ALG9)
PADL: 103921550(ALG9)
AAM: 106495786(ALG9)
NPD: 112948713(ALG9)
ASN: 102374651(ALG9)
AMJ: 102562344(ALG9)
CPOO: 109318072(ALG9)
GGN: 109291493(ALG9)
PSS: 102459895(ALG9)
CMY: 102934700(ALG9)
TST: 117868612
CABI: 116829298(ALG9)
ACS: 100554974(alg9)
PVT: 110079382(ALG9)
PBI: 103048052(ALG9)
PMUR: 107284510
TSR: 106554244(ALG9)
PGUT: 117670246(ALG9)
PMUA: 114585145(ALG9)
ZVI: 118096865(ALG9)
GJA: 107114148(ALG9)
XLA: 108696484(alg9.L)
XTR: 100497477(alg9)
NPR: 108793088(ALG9)
DRE: 393598(alg9)
SRX: 107743811(alg9) 107745128
SANH: 107663757(alg9) 107691744
CAUA: 113049793(alg9) 113081941
IPU: 108259968(alg9)
PHYP: 113531563(alg9)
AMEX: 103032018(alg9)
EEE: 113591364(alg9)
TRU: 101070455(alg9)
LCO: 104929930(alg9)
NCC: 104964653(alg9)
CGOB: 115019463(alg9)
ELY: 117272094(alg9)
PLEP: 121952396(alg9)
SLUC: 116064870(alg9)
ECRA: 117955163(alg9)
PFLV: 114566343(alg9)
GAT: 120821358(alg9)
MSAM: 119895102(alg9)
CUD: 121519342(alg9)
MZE: 101486449(alg9)
ONL: 100708529(alg9)
OAU: 116336194(alg9)
OLA: 101170535(alg9)
OML: 112142725(alg9)
XMA: 102234922(alg9)
XCO: 114153569(alg9)
XHE: 116728294(alg9)
PRET: 103475466(alg9)
CVG: 107089829(alg9)
CTUL: 119775875(alg9)
NFU: 107386374(alg9)
KMR: 108236893(alg9)
ALIM: 106514013
AOCE: 111580640
CSEM: 103395152(alg9)
POV: 109628813(alg9)
LCF: 108893576(alg9)
SDU: 111234388(alg9)
SLAL: 111666248(alg9)
XGL: 120803122(alg9)
HCQ: 109517898(alg9)
BPEC: 110158466(alg9)
MALB: 109959297(alg9)
SASA: 106604066(alg9)
OTW: 112228222(alg9)
OMY: 110533251(alg9)
SALP: 112073179
SNH: 120043441(alg9)
ELS: 105005634(alg9)
SFM: 108936273(alg9)
PKI: 111844812(alg9)
AANG: 118234963(alg9)
LOC: 102698586(alg9)
PSPA: 121309385(alg9)
LCM: 102351899(ALG9)
CMK: 103187683(alg9)
RTP: 109931078(alg9)
BFO: 118427851
BBEL: 109466947
CIN: 100176497
SCLV: 120342689
SPU: 594288
APLC: 110974013
SKO: 100376897
DME: Dmel_CG11851(Alg9)
DER: 6555385
DSE: 6619679
DSI: Dsimw501_GD21188(Dsim_GD21188)
DAN: 6500334
DSR: 110186452
DPE: 6594673
DMN: 108156257
DWI: 6651438
DGR: 6568311
DAZ: 108608600
DNV: 115564381
DHE: 111598532
DVI: 6629674
CCAT: 101453211
MDE: 101887646
LCQ: 111679372
ACOZ: 120950057
AARA: 120908352
AME: 413917
ACER: 108003567
BIM: 100741902
BBIF: 117204620
BTER: 100642493
CCAL: 108622699
OBB: 114873475
MGEN: 117219209
NMEA: 116432978
CGIG: 122401183
SOC: 105200492
MPHA: 105833110
AEC: 105145211
ACEP: 105617805
PBAR: 105429817
VEM: 105568657
HST: 105191785
DQU: 106743368
CFO: 105256117
FEX: 115235299
LHU: 105677963
PGC: 109857165
OBO: 105284329
PCF: 106793272
NVI: 100113941
CSOL: 105365187
MDL: 103569372
CCIN: 107264195
TCA: 659725
DPA: 109538694
ATD: 109604351
AGB: 108912877
LDC: 111502263
NVL: 108556872
PPYR: 116171277
OTU: 111424319
BMOR: 101739008
BMAN: 114250300
MSEX: 115455594
BANY: 112054691
PMAC: 106711022
PPOT: 106111162
PXU: 106114583
PRAP: 110993479
ZCE: 119830324
HAW: 110383088
TNL: 113492969
PXY: 105383685
API: 115033521
DNX: 107162663
AGS: 114126237
RMD: 113552813
BTAB: 109039452
DCI: 113467602
CLEC: 106661349
HHAL: 106685159
ZNE: 110836871
CSEC: 111863191
FCD: 110851910
DMK: 116916159
PVM: 113823789
HAME: 121872571
HAZT: 108679057
EAF: 111712890
DSV: 119436175
RSAN: 119371771
RMP: 119177138
VDE: 111254568
VJA: 111263658
TUT: 107369283
DPTE: 113795045
CSCU: 111635556
PTEP: 107446992
SDM: 118188347
CEL: CELE_C14A4.3(algn-9)
CBR: CBG03061
BMY: BM_BM2182(Bm2182)
TSP: Tsp_09458
PCAN: 112554801
GAE: 121374444
CRG: 105329206
MYI: 110464260
PMAX: 117342455
OBI: 106873572
LAK: 106177199
NVE: 5506634
EPA: 110243198
ATEN: 116286401
AMIL: 114951882
PDAM: 113677314
SPIS: 111338847
DGT: 114524535
HMG: 100204971
AQU: 100632605
ATH: AT1G16900(EBS3)
ALY: 9328989
CRB: 17900491
BOE: 106343495
THJ: 104812323
CPAP: 110810918
CIT: 102608844
TCC: 18594174
GRA: 105782239
GAB: 108463917
EGR: 104448532
VRA: 106759927
VAR: 108334370
VUN: 114186041
CCAJ: 109811932
APRC: 113856060
CAM: 101498221
LJA: Lj3g3v1061380.1(Lj3g3v1061380.1) Lj4g3v0548750.1(Lj4g3v0548750.1)
ADU: 107478412
AIP: 107630061
LANG: 109325797
FVE: 101310297
RCN: 112165471
PPER: 18780426
PMUM: 103326101
PAVI: 110758193
PDUL: 117626559
ZJU: 107432495
MNT: 21401627
CSV: 101208085
CMO: 103501811
BHJ: 120084470
MCHA: 111011108
CMAX: 111476858
CMOS: 111443599
RCU: 8282188
JCU: 105639214
MESC: 110611957
POP: 7455950
PALZ: 118038313
JRE: 109012295
QLO: 115955470
VVI: 100245221
VRI: 117909491
SLY: 101267427
SPEN: 107025926
SOT: 102605628
CANN: 107878608
NSY: 104214917
NTO: 104101423
NAU: 109229180
INI: 109188434
ITR: 116011430
SIND: 105176939
EGT: 105970434
HAN: 110909113
LSV: 111893403
CCAV: 112500233
DCR: 108217638
BVG: 104891397
SOE: 110789580
NNU: 104611597
MING: 122073902
NCOL: 116267236
OSA: 4325533
DOSA: Os01t0209000-01(Os01g0209000)
OBR: 102707263
BDI: 100845981
ATS: 109757002
SBI: 8081425
ZMA: 100282615(cl19880_1)
SITA: 101765286
PHAI: 112895514
PDA: 103723054
EGU: 105040617
MUS: 103987811
DCT: 110109435
PEQ: 110033732
AOF: 109822740
ATR: 18442118
SMO: SELMODRAFT_181974(GT22A2)
PPP: 112283768
APRO: F751_3872
SCE: YNL219C(ALG9)
ERC: Ecym_6468
KMX: KLMA_50609(ALG9)
NCS: NCAS_0G01440(NCAS0G01440)
NDI: NDAI_0F03720(NDAI0F03720)
TPF: TPHA_0P01690(TPHA0P01690)
TBL: TBLA_0G00270(TBLA0G00270)
TDL: TDEL_0A00670(TDEL0A00670)
KAF: KAFR_0A07890(KAFR0A07890)
PIC: PICST_54322(ALG9)
CAL: CAALFM_C107250CA(ALG9)
SLB: AWJ20_1264(ALG9)
NCR: NCU04454
NTE: NEUTE1DRAFT60663(NEUTE1DRAFT_60663)
MGR: MGG_14835
SSCK: SPSK_08850
MAW: MAC_01250
MAJ: MAA_06273
CMT: CCM_00885
BFU: BCIN_02g04450(Bcalg9)
MBE: MBM_02909
ANI: AN0729.2
ANG: ANI_1_984074(An08g07020)
CIM: CIMG_13706(CIMG03381)
ABE: ARB_05187
TVE: TRV_03601
PTE: PTT_15334
SPO: SPAC1834.05(alg9)
CNE: CNF00920
CNB: CNBF3770
TASA: A1Q1_06816
ABP: AGABI1DRAFT67279(AGABI1DRAFT_67279)
ABV: AGABI2DRAFT213830(AGABI2DRAFT_213830)
MGL: MGL_3788
MRT: MRET_0592
MSYM: MSY001_1844(ALG9.1)
DDI: DDB_G0279349(alg9)
DFA: DFA_11809(alg9)
NGD: NGA_0640700(ALG9)
SPAR: SPRG_15755
LMA: LMJF_12_0160(ALG9)
LIF: LINJ.12.0140(ALG9)
 » show all
Reference
1  [PMID:19451548]
  Authors
Vleugels W, Keldermans L, Jaeken J, Butters TD, Michalski JC, Matthijs G, Foulquier F
  Title
Quality control of glycoproteins bearing truncated glycans in an ALG9-defective (CDG-IL) patient.
  Journal
Glycobiology 19:910-7 (2009)
DOI:10.1093/glycob/cwp067
Reference
2  [PMID:10660594]
  Authors
Cipollo JF, Trimble RB
  Title
The accumulation of Man(6)GlcNAc(2)-PP-dolichol in the Saccharomyces cerevisiae Deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm  addition in downstream oligosaccharide-lipid and glycoprotein glycan processing.
  Journal
J Biol Chem 275:4267-77 (2000)
DOI:10.1074/jbc.275.6.4267
Reference
3  [PMID:15987956]
  Authors
Frank CG, Aebi M.
  Title
ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis.
  Journal
Glycobiology 15:1156-63 (2005)
DOI:10.1093/glycob/cwj002
  Sequence
[sce:YNL219C]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.259
IUBMB Enzyme Nomenclature: 2.4.1.259
ExPASy - ENZYME nomenclature database: 2.4.1.259
BRENDA, the Enzyme Database: 2.4.1.259

DBGET integrated database retrieval system