KEGG ENZYME: 2.4.1.260

ENZYME: 2.4.1.260

Entry

EC 2.4.1.260 Enzyme

Name

dolichyl-P-Man:Man7GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase;
ALG12;
ALG12 mannosyltransferase;
ALG12 alpha1,6mannosyltransferase;
dolichyl-P-mannose:Man7GlcNAc2-PP-dolichyl mannosyltransferase;
dolichyl-P-Man:Man7GlcNAc2-PP-dolichyl alpha6-mannosyltransferase;
EBS4;
Dol-P-Man:Man7GlcNAc2-PP-Dol alpha-1,6-mannosyltransferase;
dolichyl beta-D-mannosyl phosphate:D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,6-mannosyltransferase

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

dolichyl beta-D-mannosyl phosphate:alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-alpha-D-Man-(1->6)]-beta-D-Man-beta-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol 6-alpha-D-mannosyltransferase (configuration-inverting)

Reaction(IUBMB)

dolichyl beta-D-mannosyl phosphate + alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-alpha-D-Man-(1->6)]-beta-D-Man-beta-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol = alpha-D-Man-alpha-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol + dolichyl phosphate [RN:R06260]

Reaction(KEGG)

R06260(G)

Substrate

dolichyl beta-D-mannosyl phosphate [CPD:C03862];
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-alpha-D-Man-(1->6)]-beta-D-Man-beta-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol

Product

alpha-D-Man-alpha-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol;
dolichyl phosphate [CPD:C00110]

Comment

The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl diphosphate. Early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9Glc-NAc2-PP-Dol on the lumenal side use dolichyl beta-D-mannosyl phosphate.

History

EC 2.4.1.260 created 1976 as EC 2.4.1.130, part transferred 2011 to EC 2.4.1.160, modified 2012

Pathway

ec00510

N-Glycan biosynthesis

ec00513

Various types of N-glycan biosynthesis

ec01100

Metabolic pathways

Orthology

K03847

alpha-1,6-mannosyltransferase

Genes

HSA:	79087(ALG12)

PTR:	470247(ALG12)

PPS:	100977641(ALG12)

GGO:	101126908(ALG12)

PON:	100431368(ALG12)

NLE:	100588258(ALG12)

MCC:	715172(ALG12)

MCF:	102117563(ALG12)

CSAB:

103223543(ALG12)

RRO:	104670360(ALG12)

RBB:	108516534(ALG12)

CJC:	100392255(ALG12)

SBQ:	101036432(ALG12)

MMU:	223774(Alg12)

RNO:	315212(Alg12)

CGE:	100770096(Alg12)

NGI:	103735462(Alg12)

HGL:	101715432(Alg12)

CCAN:

109697709(Alg12)

OCU:	100346344(ALG12)

TUP:	102492059(ALG12)

CFA:	481196(ALG12)

AML:	100477708(ALG12)

UMR:	103674123(ALG12)

ORO:	101363058(ALG12)

FCA:	101099567(ALG12)

AJU:	106987628(ALG12)

BTA:	100125314(ALG12)

BOM:	102281651(ALG12)

PHD:	102330821(ALG12)

CHX:	102181760(ALG12)

OAS:	101112896(ALG12)

SSC:	100520494(ALG12)

CFR:	102524349(ALG12)

CDK:	105090366(ALG12)

BACU:

103004635(ALG12)

LVE:	103079764(ALG12)

OOR:	101285549(ALG12)

ECB:	100146652(ALG12)

EPZ:	103565594(ALG12)

EAI:	106845994(ALG12)

MYB:	102251651(ALG12)

MYD:	102767133(ALG12)

HAI:	109396645(ALG12)

RSS:	109434905(ALG12)

PALE:

102883747(ALG12)

LAV:	100661791(ALG12)

TMU:

101345042

SHR:	100920134(ALG12)

OAA:	100073873(ALG12)

GGA:	417733(ALG12)

MGP:	100538714(ALG12)

CJO:	107308331(ALG12)

APLA:

101790290(ALG12)

ACYG:

106040489(ALG12)

TGU:	100228059(ALG12)

GFR:	102041702(ALG12)

FAB:	101811748(ALG12)

PHI:	102108073(ALG12)

PMAJ:

107204336(ALG12)

CCAE:

111923041(ALG12)

CCW:	104686842(ALG12)

FPG:	101921937(ALG12)

FCH:	102049527(ALG12)

CLV:	102086330(ALG12)

EGZ:	104134412(ALG12)

AAM:	106495380(ALG12)

ASN:	102370274(ALG12)

AMJ:	102563831(ALG12)

PSS:	102447329(ALG12)

CMY:	102931282(ALG12)

CPIC:

101946366(ALG12)

ACS:	100560474(alg12)

PVT:	110076228(ALG12)

PBI:	103051026(ALG12)

GJA:	107112025(ALG12)

XLA:	108711164(alg12.L)

XTR:	548874(alg12)

NPR:	108796727(ALG12)

DRE:	569494(alg12)

SRX:	107730685(alg12)

SANH:

107699370(alg12)

SGH:	107601012(alg12)

CCAR:

109055011 109088012 109100623

IPU:	108279802(alg12)

AMEX:

103044448(alg12)

TRU:	101076670(alg12)

TNG:	GSTEN00012989G001 GSTEN00012990G001

LCO:	104926575(alg12)

NCC:	104953716(alg12)

MZE:	101476903(alg12)

OLA:	101171875(alg12)

XMA:	102222592(alg12)

PRET:

108165888(alg12)

NFU:	107383916(alg12)

KMR:	108240986(alg12)

CSEM:

103382918(alg12)

LCF:	108899853(alg12)

SDU:	111224930(alg12)

HCQ:	109523664(alg12)

BPEC:

110161826(alg12)

MALB:

109960949(alg12)

SASA:

100195409(alg12) 106609155

OTW:	112216768(alg12)

ELS:	105020252(alg12)

SFM:	108931614(alg12)

LCM:	102363795(ALG12)

CMK:	103177032(alg12)

BFO:	BRAFLDRAFT_72229

CIN:

SPU:

APLC:

SKO:

DME:	Dmel_CG8412(CG8412)

DPO:	Dpse_GA21058

DAN:	Dana_GF23035

DSE:	Dsec_GM23833

DSI:	Dsimw501_GD18643(Dsim_GD18643)

DYA:	Dyak_GE25981

DGR:	Dgri_GH17417

DMO:	Dmoj_GI22746

DVI:	Dvir_GJ22748

MDE:

101900007

AGA:	AgaP_AGAP000102

AAG:

5564018

CQU:	CpipJ_CPIJ009375

AME:

BIM:

BTER:

SOC:

AEC:

ACEP:

PBAR:

HST:

DQU:

CFO:

LHU:

PGC:

PCF:

NVI:

MDL:

TCA:

DPA:

NVL:

BMOR:

DPL:	KGM_214579

PMAC:

PRAP:

HAW:

PXY:

API:

DNX:

CLEC:

PHU:	Phum_PHUM333940

ZNE:

110831686

FCD:

110842961

DPX:	DAPPUDRAFT_307223 DAPPUDRAFT_340335

ISC:	IscW_ISCW010680

TUT:

107359154

CEL:	CELE_ZC513.5(ZC513.5)

CBR:

CBG11382

NAI:	NECAME_10496

BMY:

Bm1_50775

LOA:	LOAG_16479

TSP:

Tsp_15122

HRO:	HELRODRAFT_70039

LGI:	LOTGIDRAFT_228830

CRG:

MYI:

OBI:

LAK:

SMM:	Smp_161590

OVI:	T265_07001

EGL:	EGR_00930 EGR_00931

NVE:	NEMVE_v1g159238

EPA:

110245274

ADF:

107341875

HMG:

100214068

TAD:	TRIADDRAFT_28195

AQU:

100640861

ATH:	AT1G02145(ALG12)

ALY:	ARALYDRAFT_887214

CRB:

17897192

CSAT:

104758180 104773533 104773734

EUS:	EUTSA_v10007459mg

BRP:	103843832 103844697

BNA:	106370825 106400825 106434177

BOE:	106294422 106308420

THJ:

104814061

CPAP:

110816214

CIT:

102622888

CIC:	CICLE_v10019843mg

TCC:

18614505

GRA:

105791709

GHI:	107900803 107929688

DZI:

111313726

EGR:

104444420

GMX:	100776070 100786127

PVU:	PHAVU_010G045600g

VRA:

106774197

VAR:

108347728

CCAJ:

109818826

MTR:	MTR_8g007345

CAM:

101509278

LJA:	Lj0g3v0165139.1(Lj0g3v0165139.1) Lj0g3v0165139.2(Lj0g3v0165139.2) Lj3g3v0594260.1(Lj3g3v0594260.1) Lj3g3v0594260.2(Lj3g3v0594260.2)

ADU:

AIP:

LANG:

FVE:

PPER:

PMUM:

PAVI:

MDM:

PXB:

ZJU:

CSV:

CMO:

MCHA:

CMAX:

CMOS:

CPEP:

RCU:

JCU:

HBR:

POP:

JRE:	109016437 109016718

VVI:

SLY:

SPEN:

SOT:

CANN:

NTA:	107790560 107800663

NSY:

NTO:

INI:

SIND:

OEU:

HAN:

LSV:

CCAV:

DCR:

BVG:

SOE:

NNU:

OSA:

DOSA:

Os04t0544900-01(Os04g0544900)

OBR:

102708889

BDI:

100830072

ATS:	109740842(LOC109740842)

SBI:

ZMA:

SITA:

PDA:

EGU:

MUS:

DCT:

PEQ:

AOF:	109839065 109839066

ATR:

18429228

SMO:	SELMODRAFT_91475(GT22A3)

PPP:

112295252

OLU:	OSTLU_43663

OTA:	OT_ostta14g02510

BPG:	Bathy13g00280

MIS:	MICPUN_63738

MPP:	MICPUCDRAFT_22710

CSL:	COCSUDRAFT_29611

CVR:	CHLNCDRAFT_8393

APRO:

F751_2283

GSL:	Gasu_59850

CCP:	CHC_T00009193001

SCE:	YNR030W(ALG12)

AGO:	AGOS_AAR043C

ERC:

Ecym_2496

KLA:	KLLA0_C08591g

KMX:	KLMA_70199(ALG12)

LTH:	KLTH0A06710g

VPO:	Kpol_1028p42

ZRO:	ZYRO0D08250g

CGR:	CAGL0M10769g

NCS:	NCAS_0A05360(NCAS0A05360)

NDI:	NDAI_0K02210(NDAI0K02210)

TPF:	TPHA_0M01820(TPHA0M01820)

TBL:	TBLA_0F04110(TBLA0F04110)

TDL:	TDEL_0A07530(TDEL0A07530)

KAF:	KAFR_0C04580(KAFR0C04580)

PPA:	PAS_chr4_0544

DHA:	DEHA2C17270g

PIC:	PICST_31100(ECM39)

PGU:	PGUG_03898

SPAA:

SPAPADRAFT_63472

LEL:	LELG_05213

CAL:	CAALFM_C702970WA(ECM39)

CTP:	CTRG_05215

COT:	CORT_0G04380

CDU:	CD36_72630

CTEN:

CANTEDRAFT_104497

YLI:	YALI0C02475g

CLU:	CLUG_04302

CLUS:

A9F13_04g02123

CAUR:

QG37_01592

SLB:	AWJ20_3629(ALG12)

NCR:

NCU07472

NTE:	NEUTE1DRAFT88888(NEUTE1DRAFT_88888)

SMP:	SMAC_05174

PAN:	PODANSg09956

TTT:	THITE_21903

MTM:	MYCTH_2295836

CTHR:

CTHT_0053460

MGR:

MGG_09909

TMN:	UCRPA7_6503

SSCK:

SPSK_08732

FGR:	FGSG_01233

FPU:	FPSE_01776

FVR:	FVEG_01362

FOX:	FOXG_00155

NHE:	NECHADRAFT_105807

TRE:	TRIREDRAFT_63828

MAW:

MAC_00572

MAJ:

MAA_07235

CMT:

CCM_06927

PLJ:	VFPFJ_03373

VDA:	VDAG_04507

CFJ:	CFIO01_04924

ELA:	UCREL1_7790

PFY:	PFICI_01052

SSL:	SS1G_10012

BFU:	BCIN_07g04680

MBE:

MBM_02529

PSCO:

LY89DRAFT_604257

GLZ:	GLAREA_00829

ANI:

AN3588.2

AFM:	AFUA_4G12900

AOR:	AO090009000318

ANG:	ANI_1_1126014(An01g08460)

AFV:	AFLA_051500

ACT:	ACLA_051860

NFI:	NFIA_103440

PCS:	Pc22g02840

PDP:	PDIP_44640

CIM:	CIMG_00617

CPW:	CPC735_056390

PBL:	PAAG_07032

PBN:	PADG_01561

URE:	UREG_00622

ABE:

ARB_00142

TVE:

TRV_01650

PNO:	SNOG_04448

PTE:

PTT_15141

BZE:	COCCADRAFT_2870

BSC:	COCSADRAFT_191517

BOR:	COCMIDRAFT_98235

AALT:

CC77DRAFT_933602

ZTR:	MYCGRDRAFT_109480

PFJ:	MYCFIDRAFT_34652

BCOM:

BAUCODRAFT_35286

NPA:	UCRNP2_3478

SPO:	SPBC1734.12c(alg12)

CNE:

CNC03430

CNB:

CNBC3790

CGI:	CGB_C5280C

TMS:	TREMEDRAFT_41756

TVS:	TRAVEDRAFT_126346

PCO:	PHACADRAFT_180449 PHACADRAFT_214615

SHS:	STEHIDRAFT_165833 STEHIDRAFT_73504

HIR:	HETIRDRAFT_165790

PSQ:	PUNSTDRAFT_120292

ADL:	AURDEDRAFT_143051

FME:	FOMMEDRAFT_131787

GTR:	GLOTRDRAFT_79634

LBC:	LACBIDRAFT_302539(ALG12)

MPR:	MPER_04378

MRR:	Moror_3413

CCI:	CC1G_11662

SCM:	SCHCODRAFT_104684

ABP:	AGABI1DRAFT75475(AGABI1DRAFT_75475)

ABV:	AGABI2DRAFT184199(AGABI2DRAFT_184199)

CPUT:

CONPUDRAFT_151427

SLA:	SERLADRAFT_467450

WSE:	WALSEDRAFT_68580

WIC:	J056_001199

UMA:	UMAG_11341

PFP:	PFL1_03512

MGL:

MGL_2261

PGR:	PGTG_03644

MLR:	MELLADRAFT_40792

MBR:	MONBRDRAFT_31470

SRE:	PTSG_11395

DDI:	DDB_G0267884(alg12)

DPP:	DICPUDRAFT_80121

DFA:	DFA_08503(alg12)

PTI:	PHATRDRAFT_44425

FCY:	FRACYDRAFT_247580

TPS:	THAPSDRAFT_262459

PIF:	PITG_10838

PSOJ:

PHYSODRAFT_469873

SPAR:

SPRG_11694 SPRG_16521

EHX:	EMIHUDRAFT_454648

GTT:	GUITHDRAFT_65500

TBR:	Tb927.2.4720

TCR:	509317.50 511621.60

NGR:	NAEGRDRAFT_29294

» show all

Reference

1 [PMID:15987956]

Authors

Frank CG, Aebi M

Title

ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis.

Journal

Glycobiology 15:1156-63 (2005)
DOI:10.1093/glycob/cwj002

Sequence

[sce:YNR030W]

Reference

2 [PMID:20023196]

Authors

Hong Z, Jin H, Fitchette AC, Xia Y, Monk AM, Faye L, Li J

Title

Mutations of an alpha1,6 mannosyltransferase inhibit endoplasmic reticulum-associated degradation of defective brassinosteroid receptors in Arabidopsis.

Journal

Plant Cell 21:3792-802 (2009)
DOI:10.1105/tpc.109.070284

Sequence

[ath:AT1G02145]

Reference

3 [PMID:12460943]

Authors

Cipollo JF, Trimble RB

Title

The Saccharomyces cerevisiae alg12delta mutant reveals a role for the middle-arm alpha1,2Man- and upper-arm alpha1,2Manalpha1,6Man- residues of Glc3Man9GlcNAc2-PP-Dol in regulating glycoprotein glycan processing in the endoplasmic reticulum and Golgi apparatus.

Journal

Glycobiology 12:749-62 (2002)

Reference

4 [PMID:12217961]

Authors

Grubenmann CE, Frank CG, Kjaergaard S, Berger EG, Aebi M, Hennet T

Title

ALG12 mannosyltransferase defect in congenital disorder of glycosylation type lg.

Journal

Hum Mol Genet 11:2331-9 (2002)
DOI:10.1093/hmg/11.19.2331

Sequence

[hsa:79087]