Entry
Name
dolichyl-P-Man:Man8GlcNAc2-PP-dolichol alpha-1,2-mannosyltransferase;
ALG9;
ALG9 alpha1,2 mannosyltransferase;
dolichylphosphomannose-dependent ALG9 mannosyltransferase;
ALG9 mannosyltransferase;
Dol-P-Man:Man8GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase;
dolichyl beta-D-mannosyl phosphate:D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol 2-alpha-D-mannosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
dolichyl beta-D-mannosyl-phosphate:alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol 2-alpha-D-mannosyltransferase (configuration-inverting)
Reaction(IUBMB)
dolichyl beta-D-mannosyl phosphate + alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol = alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol + dolichyl phosphate [RN:
R06261 ]
Reaction(KEGG)
Substrate
dolichyl beta-D-mannosyl phosphate [CPD:
C03862 ];
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
Product
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol;
dolichyl phosphate [CPD:
C00110 ]
Comment
The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl diphosphate. Early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9Glc-NAc2-PP-Dol on the lumenal side use dolichyl beta-D-mannosyl phosphate. ALG9 mannosyltransferase catalyses the addition of two different alpha-1,2-mannose residues: the addition of alpha-1,2-mannose to Man6GlcNAc2-PP-Dol (EC
2.4.1.259 ) and the addition of alpha-1,2-mannose to Man8GlcNAc2-PP-Dol (EC
2.4.1.261 ).
History
EC 2.4.1.261 created 1976 as EC 2.4.1.130, part transferred 2011 to EC 2.4.1.261, modified 2012
Pathway
ec00513 Various types of N-glycan biosynthesis
Orthology
K03846 alpha-1,2-mannosyltransferase
Genes
CSYR : 103256337 103268036
SRX : 107743811(alg9) 107745128
SANH : 107663757(alg9) 107691744
CCAR : 109072865 109088011
CAUA : 113049793(alg9) 113081941
CGIB : 127958613 127994526
MASI : 127426852 127439859
TNG : GSTEN00000557G001 GSTEN00018965G001
ARUT : 117397108 117397360
LPIC : 129278466 129279366
DSI : Dsimw501_GD21188(Dsim_GD21188)
AALB : 109416624 109432833 115261554
APLN : 108732966 112906412
CEL : CELE_C14A4.3(algn-9)
CRQ : GCK72_005497 GCK72_005498 GCK72_016230 GCK72_016231
SHX : MS3_00003435(ALG9_1)
CSAT : 104740375 104756015 104775791
BNA : 106346739 106365573 106387601 106411958
HSYR : 120123262 120161409 120198367
DZI : 111275903 111276254 111298853
MSYL : 126589925 126615855
PXB : 103926890 103932217 103934268
CPEP : 111787754 111796738
CAVE : 132174893 132175816
SSPL : 121783551 121805358 121806194
SHIS : 125196502 125213175
CSIN : 114260554 114262824
PSOM : 113277420 113336299
TAES : 123060631 123069253 123077768
MFLO : 136457446 136531123
PVIR : 120673101 120705630
SMO : SELMODRAFT_181974(GT22A2)
CGR : 2890500(GVI51_K04851)
NCS : NCAS_0G01440(NCAS0G01440)
NDI : NDAI_0F03720(NDAI0F03720)
TPF : TPHA_0P01690(TPHA0P01690)
TBL : TBLA_0G00270(TBLA0G00270)
TDL : TDEL_0A00670(TDEL0A00670)
KAF : KAFR_0A07890(KAFR0A07890)
KNG : KNAG_0F00920(KNAG0F00920)
LBG : 92205751(LODBEIA_P05550)
CAL : CAALFM_C107250CA(ALG9)
YLI : 2911871(YALI2_E00579g)
NTE : NEUTE1DRAFT60663(NEUTE1DRAFT_60663)
SMP : 10807767(SMAC4_01154)
TATV : 25775100(TrAtP1_007631)
TASP : 36608843(TrAFT101_007796)
MAW : 19245561(J3458_001731)
PLJ : 28888520(PLICBS_003703)
CDET : 87947257(CDEST_10757)
BFU : BCIN_02g04450(Bcalg9)
CIM : CIMG_13706(CIMG03381)
PTRR : 6349976(PtrM4_030430)
CDEP : 91087222(L203_103011)
KMG : 30167897(I203_100177)
KNE : 92184220(IAR55_006962)
CCAC : CcaHIS019_0501310(ALG9)
ABP : AGABI1DRAFT67279(AGABI1DRAFT_67279)
ABV : AGABI2DRAFT213830(AGABI2DRAFT_213830)
SCM : SCHCO_02610799(SCHCODRAFT_02610799)
MSYM : MSY001_1844(ALG9.1)
FCY : FRACYDRAFT_196804(MT2)
TCR : 507603.150 509429.220
LMAT : 92517694(LSCM1_07826)
LOI : 92363423(LSCM4_07607)
LEIS : 94183507(GH5_08301)
LENR : 94175383(CUR178_08243)
LEIN : 94191875(JIQ42_08318)
» show all
Taxonomy
Reference
Authors
Vleugels W, Keldermans L, Jaeken J, Butters TD, Michalski JC, Matthijs G, Foulquier F
Title
Quality control of glycoproteins bearing truncated glycans in an ALG9-defective (CDG-IL) patient.
Journal
Reference
Authors
Frank CG, Aebi M.
Title
ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 2.4.1.261