KEGG ENZYME: 2.4.1.264

ENZYME: 2.4.1.264

Entry

EC 2.4.1.264                Enzyme

Name

D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronosyltransferase;
GumK;
UDP-glucuronate:D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol beta-1,2-glucuronyltransferase;
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-alpha-D-glucuronate:alpha-D-Man-(1->3)-beta-D-Glc-(1->4)-alpha-D-Glc-1-diphospho-ditrans,octacis-undecaprenol beta-1,2-glucuronosyltransferase (configuration-inverting)

Reaction(IUBMB)

UDP-alpha-D-glucuronate + alpha-D-Man-(1->3)-beta-D-Glc-(1->4)-alpha-D-Glc-1-diphospho-ditrans,octacis-undecaprenol = UDP + beta-D-GlcA-(1->2)-alpha-D-Man-(1->3)-beta-D-Glc-(1->4)-alpha-D-Glc-1-diphospho-ditrans,octacis-undecaprenol [RN:R09732]

Reaction(KEGG)

R09732

Substrate

UDP-alpha-D-glucuronate [CPD:C00167];
alpha-D-Man-(1->3)-beta-D-Glc-(1->4)-alpha-D-Glc-1-diphospho-ditrans,octacis-undecaprenol

Product

UDP [CPD:C00015];
beta-D-GlcA-(1->2)-alpha-D-Man-(1->3)-beta-D-Glc-(1->4)-alpha-D-Glc-1-diphospho-ditrans,octacis-undecaprenol

Comment

The enzyme is involved in the biosynthesis of the exopolysaccharides xanthan (in the bacterium Xanthomonas campestris) and acetan (in the bacterium Gluconacetobacter xylinus).

History

EC 2.4.1.264 created 2011, modified 2016

Orthology

K13659

2-beta-glucuronyltransferase

Genes

XFA:

XF_2361

XFT:	PD_1388(gumK)

XFM:	Xfasm12_1530

XFN:	XfasM23_1474

XFF:	XFLM_01075

XFL:	P303_06655

XFS:	D934_11035

XFH:	XFHB_03295

XTW:	AB672_04685

XCC:	XCC2445(gumK)

XCB:

XC_1667

XCA:	xcc-b100_1721(gumK)

XCP:	XCR_2758(gumK)

XCV:	XCV2778(gumK)

XAX:	XACM_2558(gumK)

XAC:	XAC2576(gumK)

XCI:	XCAW_02257(gumK)

XCT:	J151_02757

XCJ:	J158_02744

XCU:	J159_02742

XCN:	J169_02754

XCW:	J162_02746

XCR:	J163_02741

XCM:	J164_02743

XCF:	J172_02748

XFU:	XFF4834R_chr26130(gumK)

XAO:	XAC29_13120

XOM:	XOO3010(XOO3010)

XOO:	XOO3170(gumK)

XOP:	PXO_01401(gumK)

XOY:	AZ54_08015

XOR:	XOC_1876(gumK)

XOZ:	BE73_08805

XTN:	FD63_08030

XFR:	BER92_06085

XVE:	BJD12_03910

XPE:	BJD13_03595

XHR:	XJ27_06760

XGA:	BI317_09575

XPH:	XppCFBP6546_19515(XppCFBP6546P_19515)

XVA:	C7V42_09290

XAN:	AC801_09290

XAR:	XB05_07770

XHY:	FZ025_16445

XCZ:	EBN15_09455

XEU:	XSP_002559

XHD:	LMG31886_17120(gumK)

XPR:	MUG10_18770

XAG:	HEP73_01778

XDY:	NYR95_14140

XAS:	HEP74_01696

XHA:	PG878_08510

XRY:	QN244_08195

PSD:	DSC_06260 DSC_13670

PWI:	MWN52_13240 MWN52_14780

MNK:	RE428_05420

CJA:	CJA_0618(gt70A)

CEB:	B0D95_08985

CELL:

CBR65_10400

CEK:	D0B88_17285

CEG:	D0C16_09515

GLC:	JQN73_13840 JQN73_17615

SPLB:

SFPGR_10350(gumK)

ABRE:

pbN1_39130

DEY:	HYN24_07370

AZO:	azo2235(gumK)

AOA:

dqs_2368

AZR:	CJ010_14190

SIX:	BSY16_5351

AVV:	RvVAT039_12250

AVF:	RvVAR031_32160

AVI:

Avi_6016

RLS:	HB780_18345

ARA:

Arad_3074

PDES:

FE840_000810 FE840_001985

MEDK:

QEV83_18795

TSO:

IZ6_26580

RPOP:

K1718_25045

SULI:

C1J05_21115

ROT:	FIV09_19275(gumK)

SAER:

NEF64_03640

SABI:

PBT88_06970

SJP:	SJA_C2-01910(gumK)

SYB:	TZ53_09480

SYA:	A6768_08600

SUFL:

FIL70_23600

SBAR:

H5V43_20185

SPPH:

KFK14_12210

GDI:	GDI2542(gumK)

GDJ:

Gdia_0782

GXY:	GLX_27750(gumK)

GXL:

H845_816

KNA:	B0W47_14415

KEU:	S101446_03142(gumK)

KRE:	GWK63_04890

KOI:	LV478_10000

AACE:

A0U92_11530

KBA:	A0U89_05135

NGU:	QN315_03685

RCE:	RC1_2535(gumK)

NOS:	Nos7107_0795

CALH:

IJ00_16875

FBT:	D770_17675

MRTJ:

KHC33_15105

» show all

Reference

1 [PMID:9537354]

Authors

Katzen F, Ferreiro DU, Oddo CG, Ielmini MV, Becker A, Puhler A, Ielpi L

Title

Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence.

Journal

J Bacteriol 180:1607-17 (1998)
DOI:10.1128/JB.180.7.1607-1617.1998

Reference

2 [PMID:7683019]

Authors

Ielpi L, Couso RO, Dankert MA

Title

Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris.

Journal

J Bacteriol 175:2490-500 (1993)
DOI:10.1128/JB.175.9.2490-2500.1993

Reference

3 [PMID:18854951]

Authors

Kim SY, Kim JG, Lee BM, Cho JY

Title

Mutational analysis of the gum gene cluster required for xanthan biosynthesis in Xanthomonas oryzae pv oryzae.

Journal

Biotechnol Lett 31:265-70 (2009)
DOI:10.1007/s10529-008-9858-3

Reference

4 [PMID:16946469]

Authors

Barreras M, Bianchet MA, Ielpi L

Title

Crystallization and preliminary crystallographic characterization of GumK, a membrane-associated glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis.

Journal

Acta Crystallogr Sect F Struct Biol Cryst Commun 62:880-3 (2006)
DOI:10.1107/S1744309106028764

Sequence

[ag:AAN86640]

Reference

5 [PMID:18596046]

Authors

Barreras M, Salinas SR, Abdian PL, Kampel MA, Ielpi L

Title

Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase.

Journal

J Biol Chem 283:25027-35 (2008)
DOI:10.1074/jbc.M801227200

Sequence

[ag:AAN86640]

Reference

6 [PMID:11841812]

Authors

Vojnov AA, Bassi DE, Daniels MJ, Dankert MA

Title

Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris.

Journal

Carbohydr Res 337:315-26 (2002)
DOI:10.1016/S0008-6215(01)00322-6

Reference

7 [PMID:14736729]

Authors

Barreras M, Abdian PL, Ielpi L

Title

Functional characterization of GumK, a membrane-associated beta-glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis.

Journal

Glycobiology 14:233-41 (2004)
DOI:10.1093/glycob/cwh056

Sequence

[ag:AAN86640]

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.264

IUBMB Enzyme Nomenclature:

2.4.1.264

ExPASy - ENZYME nomenclature database:

2.4.1.264

BRENDA, the Enzyme Database:

2.4.1.264

DBGET integrated database retrieval system