Entry
Name
dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase;
ALG8;
Dol-P-Glc:Glc1Man9GlcNAc2-PP-Dol alpha-1,3-glucosyltransferase;
dolichyl beta-D-glucosyl phosphate:D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,3-glucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
dolichyl beta-D-glucosyl-phosphate:alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol 3-alpha-D-glucosyltransferase (configuration-inverting)
Reaction(IUBMB)
dolichyl beta-D-glucosyl phosphate + alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol = alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol + dolichyl phosphate [RN:
R06263 ]
Reaction(KEGG)
Substrate
dolichyl beta-D-glucosyl phosphate [CPD:
C01246 ];
alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
Product
alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol;
dolichyl phosphate [CPD:
C00110 ]
Comment
The successive addition of three glucose residues by EC
2.4.1.267 (dolichyl-P-Glc:Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase), EC
2.4.1.265 and EC
2.4.1.256 (dolichyl-P-Glc:Glc2Man9GlcNAc2-PP-dolichol alpha-1,2-glucosyltransferase) represents the final stage of the lipid-linked oligosaccharide assembly.
History
EC 2.4.1.265 created 2011, modified 2012
Pathway
Orthology
K03849 alpha-1,3-glucosyltransferase
Genes
MORG : 121448380 121458942
AAM : 106493406(ALG8) 106495477
DCC : 119858322(ALG8) 119858336
CCAR : 109079660 109084578
CAUA : 113038602 113064292
CGIB : 127941842 127986535
SLAL : 111648939 111653008
ARUT : 117405391 117406795
DSI : Dsimw501_GD16816(Dsim_GD16816)
TDA : 119664559 119666946 119667243
AALB : 109399851 115267053
CFEL : 113373707 113373712 113382126
PPOI : 119091319 119092548
CEL : CELE_C08H9.3(algn-8)
RPHI : 132712870 132737177
SHX : MS3_00007322(ALG8_1)
CSAT : 104783018 104784871 104793415
HSYR : 120114904 120118408 120186581
PCIN : 129290245 129295868
CSAV : 115710680 115722880
SSPL : 121761353 121765754
AEW : 130774531 130780934 130792620
PSOM : 113297322 113302111
TAES : 123128032 123137815 123145130
MFLO : 136473712 136474597 136497973
PVIR : 120656847 120712908
SMO : SELMODRAFT_129281(GT57A2)
CGR : 2891072(GVI51_L10395)
NCS : NCAS_0G00450(NCAS0G00450)
NDI : NDAI_0F00510(NDAI0F00510)
TPF : TPHA_0J01380(TPHA0J01380)
TBL : TBLA_0A09310(TBLA0A09310)
TDL : TDEL_0B02540(TDEL0B02540)
KAF : KAFR_0D02440(KAFR0D02440)
KNG : KNAG_0L01920(KNAG0L01920)
LBG : 92208554(LODBEIA_P33580)
CAL : CAALFM_C301880WA(ALG8)
YLI : 2905848(YALI2_A00429g)
NTE : NEUTE1DRAFT63156(NEUTE1DRAFT_63156)
SMP : 10808097(SMAC4_02262)
FPOA : FPOAC1_002650(ALG8)
TATV : 25785153(TrAtP1_009642)
PLJ : 28886950(PLICBS_002263)
PTRR : 6347681(PtrM4_126070)
LBC : LACBIDRAFT_311682(ALG8)
ABP : AGABI1DRAFT73545(AGABI1DRAFT_73545)
ABV : AGABI2DRAFT202561(AGABI2DRAFT_202561)
SCM : SCHCO_02622631(SCHCODRAFT_02622631)
» show all
Taxonomy
Reference
Authors
Stagljar I, te Heesen S, Aebi M
Title
New phenotype of mutations deficient in glucosylation of the lipid-linked oligosaccharide: cloning of the ALG8 locus.
Journal
Sequence
Reference
Authors
Runge KW, Robbins PW
Title
A new yeast mutation in the glucosylation steps of the asparagine-linked glycosylation pathway. Formation of a novel asparagine-linked oligosaccharide containing two glucose residues.
Journal
J Biol Chem 261:15582-90 (1986)
Sequence
Reference
Authors
Chantret I, Dancourt J, Dupre T, Delenda C, Bucher S, Vuillaumier-Barrot S, Ogier de Baulny H, Peletan C, Danos O, Seta N, Durand G, Oriol R, Codogno P, Moore SE
Title
A deficiency in dolichyl-P-glucose:Glc1Man9GlcNAc2-PP-dolichyl alpha3-glucosyltransferase defines a new subtype of congenital disorders of glycosylation.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 2.4.1.265