KEGG   ENZYME: 2.4.1.265Help
Entry
EC 2.4.1.265                Enzyme                                 

Name
dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase;
ALG8;
Dol-P-Glc:Glc1Man9GlcNAc2-PP-Dol alpha-1,3-glucosyltransferase;
dolichyl beta-D-glucosyl phosphate:D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,3-glucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
dolichyl beta-D-glucosyl phosphate:alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol 3-alpha-D-glucosyltransferase (configuration-inverting)
Reaction(IUBMB)
dolichyl beta-D-glucosyl phosphate + alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol = alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol + dolichyl phosphate [RN:R06263]
Reaction(KEGG)
R06263(G)
Show
Substrate
dolichyl beta-D-glucosyl phosphate [CPD:C01246];
alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
Product
alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol;
dolichyl phosphate [CPD:C00110]
Comment
The successive addition of three glucose residues by EC 2.4.1.267 (dolichyl-P-Glc:Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase), EC 2.4.1.265 and EC 2.4.1.256 (dolichyl-P-Glc:Glc2Man9GlcNAc2-PP-dolichol alpha-1,2-glucosyltransferase) represents the final stage of the lipid-linked oligosaccharide assembly.
History
EC 2.4.1.265 created 2011, modified 2012
Pathway
ec00510  N-Glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K03849  alpha-1,3-glucosyltransferase
Genes
HSA: 79053(ALG8)
PTR: 451446(ALG8)
PPS: 100976025(ALG8)
GGO: 101143793(ALG8)
PON: 100172957(ALG8)
NLE: 100583648(ALG8)
MCC: 699834(ALG8)
MCF: 102118129(ALG8)
CSAB: 103248107(ALG8)
RRO: 104664304(ALG8)
RBB: 108543192(ALG8)
CJC: 100410610(ALG8)
SBQ: 101046030(ALG8)
MMU: 381903(Alg8)
RNO: 293129(Alg8)
CGE: 100766150(Alg8)
NGI: 103742996(Alg8)
HGL: 101717763(Alg8)
CCAN: 109683794(Alg8)
OCU: 100350058(ALG8)
TUP: 102489591(ALG8)
CFA: 476793(ALG8)
AML: 100467813(ALG8)
UMR: 103678139(ALG8)
ORO: 101368757(ALG8)
FCA: 101080479(ALG8)
PTG: 102966535(ALG8)
AJU: 106980813(ALG8)
BTA: 538731(ALG8)
BOM: 102278012(ALG8)
BIU: 109554316(ALG8)
PHD: 102325804(ALG8)
CHX: 102177868(ALG8)
OAS: 101109656(ALG8)
SSC: 100511030(ALG8)
CFR: 102517495(ALG8)
CDK: 105097543(ALG8)
BACU: 103014128(ALG8)
LVE: 103079942(ALG8)
OOR: 101269252(ALG8)
ECB: 100051152(ALG8)
EPZ: 103541459(ALG8)
EAI: 106842888(ALG8)
MYB: 102252684(ALG8)
MYD: 102758490(ALG8)
HAI: 109371245(ALG8)
RSS: 109444765(ALG8)
PALE: 102886309(ALG8)
LAV: 100664865(ALG8)
TMU: 101345559
MDO: 100027315(ALG8)
SHR: 100925953(ALG8)
OAA: 100090090(ALG8)
GGA: 428097(ALG8)
MGP: 100542312(ALG8)
CJO: 107308508(ALG8)
APLA: 101792588(ALG8)
ACYG: 106039860(ALG8)
TGU: 100232627(ALG8)
GFR: 102036920(ALG8)
FAB: 101817964(ALG8)
PHI: 102099307(ALG8)
PMAJ: 107208102(ALG8)
CCW: 104690297(ALG8)
CLV: 102090337(ALG8)
EGZ: 104124754(ALG8)
AAM: 106493406(ALG8) 106495477
ASN: 102377483(ALG8)
AMJ: 102569717(ALG8)
PSS: 102458309(ALG8)
CMY: 102937209(ALG8)
CPIC: 101940096(ALG8)
ACS: 100567213(alg8)
PVT: 110088571(ALG8)
PBI: 103055334(ALG8)
GJA: 107115030(ALG8)
XLA: 431849(alg8.S)
XTR: 548388(alg8)
NPR: 108794639(ALG8)
DRE: 327601(alg8)
SANH: 107703477
IPU: 108278741(alg8)
AMEX: 103040022(alg8)
TRU: 101076514(alg8)
LCO: 104926121(alg8)
MZE: 101476826(alg8)
OLA: 101162467(alg8)
XMA: 102229183(alg8)
PRET: 103459846(alg8)
NFU: 107396661(alg8)
CSEM: 103395644(alg8)
LCF: 108891322(alg8)
HCQ: 109512436(alg8)
BPEC: 110159840(alg8)
SASA: 106602843(alg8)
ELS: 105006051(alg8)
SFM: 108934242(alg8)
LCM: 102363012(ALG8)
CMK: 103177229(alg8)
CIN: 101242594
SPU: 581546
APLC: 110973227
SKO: 102807371
DME: Dmel_CG4542(xit)
DSI: Dsimw501_GD16816(Dsim_GD16816)
MDE: 101896220
AAG: 5580305
AME: 551841
BIM: 100740504
BTER: 100644277
SOC: 105196202
AEC: 105153200
ACEP: 105619256
PBAR: 105427323
HST: 105186694
CFO: 105257482
LHU: 105677029
PGC: 109855095
NVI: 100121119
TCA: 664351
DPA: 109538141
NVL: 108560432
BMOR: 101742022
PMAC: 106708744
PRAP: 110997988
PXY: 105385645
API: 103309064
DNX: 107163044
ZNE: 110830530
FCD: 110863056
TUT: 107361038
CEL: CELE_C08H9.3(C08H9.3)
CBR: CBG00995
BMY: Bm1_02530
TSP: Tsp_09152
CRG: 105346207
MYI: 110448445
OBI: 106869268
SHX: MS3_02179
EPA: 110249495
ADF: 107339921
HMG: 100199195
ATH: AT2G44660
CRB: 17888972
BRP: 103866821
BOE: 106341204
THJ: 104817180
CPAP: 110815024
CIT: 102612225
TCC: 18589510
GRA: 105788025
DZI: 111285204
EGR: 104444500
GMX: 100802149
VRA: 106755478
CCAJ: 109819271
ADU: 107482396
AIP: 107638251
LJA: Lj0g3v0181409.1(Lj0g3v0181409.1) Lj0g3v0280879.1(Lj0g3v0280879.1) Lj4g3v2479510.1(Lj4g3v2479510.1)
LANG: 109363047
FVE: 101293644
PMUM: 103331498
PAVI: 110764171
PXB: 103960233
CSV: 101219615
CMO: 103498979
MCHA: 111022825
CMAX: 111499081
CMOS: 111460108
CPEP: 111807545
RCU: 8279137
JCU: 105643253
HBR: 110635796
POP: 18105112
JRE: 109002243
VVI: 100253120
SLY: 101247658
SPEN: 107011146
SOT: 102587839
CANN: 107844374
NSY: 104214391
NTO: 104098538
INI: 109182532
SIND: 105173449
OEU: 111386997
HAN: 110921289
LSV: 111914502
DCR: 108193387
BVG: 104905225
SOE: 110804467
NNU: 104609859
OSA: 4330363
DOSA: Os02t0688500-01(Os02g0688500)
OBR: 102718622
BDI: 100828196
ATS: 109779710(LOC109779710)
SBI: 8078384
ZMA: 103627568
SITA: 101774641
PDA: 103710756
EGU: 105043810
DCT: 110104760
AOF: 109838493
ATR: 18427193
SMO: SELMODRAFT_129281(GT57A2)
PPP: 112291898
CRE: CHLREDRAFT_104640(GTR20)
MNG: MNEG_1654
APRO: F751_1885
SCE: YOR067C(ALG8)
ERC: Ecym_4051
KMX: KLMA_30269(ALG8)
NCS: NCAS_0G00450(NCAS0G00450)
NDI: NDAI_0F00510(NDAI0F00510)
TPF: TPHA_0J01380(TPHA0J01380)
TBL: TBLA_0A09310(TBLA0A09310)
TDL: TDEL_0B02540(TDEL0B02540)
KAF: KAFR_0D02440(KAFR0D02440)
CAL: CAALFM_C301880WA(ALG8)
CAUR: QG37_02854
NCR: NCU00163
NTE: NEUTE1DRAFT63156(NEUTE1DRAFT_63156)
MGR: MGG_05686
MAW: MAC_04090
MAJ: MAA_08949
CMT: CCM_01588
MBE: MBM_03351
ANI: AN7301.2
ANG: ANI_1_1310184(An04g08820)
ABE: ARB_03271
TVE: TRV_02500
PTE: PTT_14370
SPO: SPAC17C9.07(alg8)
LBC: LACBIDRAFT_311682(ALG8)
ABP: AGABI1DRAFT73545(AGABI1DRAFT_73545)
ABV: AGABI2DRAFT202561(AGABI2DRAFT_202561)
MGL: MGL_0397
DDI: DDB_G0275261(alg8)
SMIN: v1.2.020099.t1(symbB.v1.2.020099.t1)
SPAR: SPRG_07320
 » show all
Taxonomy
Reference
1  [PMID:8016100]
  Authors
Stagljar I, te Heesen S, Aebi M
  Title
New phenotype of mutations deficient in glucosylation of the lipid-linked oligosaccharide: cloning of the ALG8 locus.
  Journal
Proc Natl Acad Sci U S A 91:5977-81 (1994)
DOI:10.1073/pnas.91.13.5977
  Sequence
[sce:YOR067C]
Reference
2  [PMID:3536907]
  Authors
Runge KW, Robbins PW
  Title
A new yeast mutation in the glucosylation steps of the asparagine-linked glycosylation pathway. Formation of a novel asparagine-linked oligosaccharide containing two glucose residues.
  Journal
J Biol Chem 261:15582-90 (1986)
  Sequence
[sce:YOR067C]
Reference
3  [PMID:12480927]
  Authors
Chantret I, Dancourt J, Dupre T, Delenda C, Bucher S, Vuillaumier-Barrot S, Ogier de Baulny H, Peletan C, Danos O, Seta N, Durand G, Oriol R, Codogno P, Moore SE
  Title
A deficiency in dolichyl-P-glucose:Glc1Man9GlcNAc2-PP-dolichyl alpha3-glucosyltransferase defines a new subtype of congenital disorders of glycosylation.
  Journal
J Biol Chem 278:9962-71 (2003)
DOI:10.1074/jbc.M211950200
  Sequence
[hsa:79053]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.265
IUBMB Enzyme Nomenclature: 2.4.1.265
ExPASy - ENZYME nomenclature database: 2.4.1.265
BRENDA, the Enzyme Database: 2.4.1.265

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