KEGG ENZYME: 2.4.1.283

ENZYME: 2.4.1.283

Entry

EC 2.4.1.283                Enzyme

Name

2-deoxystreptamine N-acetyl-D-glucosaminyltransferase;
btrM (gene name);
neoD (gene name);
kanF (gene name)

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-N-acetyl-alpha-D-glucosamine:2-deoxystreptamine N-acetyl-D-glucosaminyltransferase

Reaction(IUBMB)

UDP-N-acetyl-alpha-D-glucosamine + 2-deoxystreptamine = UDP + 2'-N-acetylparomamine [RN:R08894]

Reaction(KEGG)

R08894

Substrate

UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
2-deoxystreptamine [CPD:C02627]

Product

UDP [CPD:C00015];
2'-N-acetylparomamine [CPD:C17582]

Comment

Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin. Unlike the enzyme from the bacterium Streptomyces kanamyceticus, which can also accept UDP-D-glucose [2] (cf. EC 2.4.1.284, 2-deoxystreptamine glucosyltransferase), the enzyme from Bacillus circulans can only accept UDP-N-acetyl-alpha-D-glucosamine [1].

History

EC 2.4.1.283 created 2012

Pathway

ec00524

Neomycin, kanamycin and gentamicin biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K13550

2-deoxystreptamine N-acetyl-D-glucosaminyltransferase / 2-deoxystreptamine glucosyltransferase

Genes

PALR:

HGI30_01160

SRN:	A4G23_05127(neoD)

SBRO:

GQF42_00970

SFB:	CP974_27360

MOM:	KUM42_19940

MSAG:

GCM10017556_22620

DVC:	Dvina_05405

Reference

1 [PMID:18311744]

Authors

Yokoyama K, Yamamoto Y, Kudo F, Eguchi T

Title

Involvement of two distinct N-acetylglucosaminyltransferases and a dual-function deacetylase in neomycin biosynthesis.

Journal

Chembiochem 9:865-9 (2008)
DOI:10.1002/cbic.200700717

Sequence

[ag:BAD95821]

Reference

2 [PMID:21983602]

Authors

Park JW, Park SR, Nepal KK, Han AR, Ban YH, Yoo YJ, Kim EJ, Kim EM, Kim D, Sohng JK, Yoon YJ

Title

Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation.

Journal

Nat Chem Biol 7:843-52 (2011)
DOI:10.1038/nchembio.671

Sequence

[ag:CAE46947]

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.283

IUBMB Enzyme Nomenclature:

2.4.1.283

ExPASy - ENZYME nomenclature database:

2.4.1.283

BRENDA, the Enzyme Database:

2.4.1.283

DBGET integrated database retrieval system