KEGG   ENZYME: 2.4.1.283
Entry
EC 2.4.1.283                Enzyme                                 

Name
2-deoxystreptamine N-acetyl-D-glucosaminyltransferase;
btrM (gene name);
neoD (gene name);
kanF (gene name)
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-N-acetyl-alpha-D-glucosamine:2-deoxystreptamine N-acetyl-D-glucosaminyltransferase
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + 2-deoxystreptamine = UDP + 2'-N-acetylparomamine [RN:R08894]
Reaction(KEGG)
R08894
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
2-deoxystreptamine [CPD:C02627]
Product
UDP [CPD:C00015];
2'-N-acetylparomamine [CPD:C17582]
Comment
Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin. Unlike the enzyme from the bacterium Streptomyces kanamyceticus, which can also accept UDP-D-glucose [2] (cf. EC 2.4.1.284, 2-deoxystreptamine glucosyltransferase), the enzyme from Bacillus circulans can only accept UDP-N-acetyl-alpha-D-glucosamine [1].
History
EC 2.4.1.283 created 2012
Pathway
ec00524  Neomycin, kanamycin and gentamicin biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13550  2-deoxystreptamine N-acetyl-D-glucosaminyltransferase / 2-deoxystreptamine glucosyltransferase
Genes
SRN: A4G23_05127(neoD)
SBRO: GQF42_00970
MSAG: GCM10017556_22620
DVC: Dvina_05405
Reference
1  [PMID:18311744]
  Authors
Yokoyama K, Yamamoto Y, Kudo F, Eguchi T
  Title
Involvement of two distinct N-acetylglucosaminyltransferases and a dual-function deacetylase in neomycin biosynthesis.
  Journal
Chembiochem 9:865-9 (2008)
DOI:10.1002/cbic.200700717
  Sequence
Reference
2  [PMID:21983602]
  Authors
Park JW, Park SR, Nepal KK, Han AR, Ban YH, Yoo YJ, Kim EJ, Kim EM, Kim D, Sohng JK, Yoon YJ
  Title
Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation.
  Journal
Nat Chem Biol 7:843-52 (2011)
DOI:10.1038/nchembio.671
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.283
IUBMB Enzyme Nomenclature: 2.4.1.283
ExPASy - ENZYME nomenclature database: 2.4.1.283
BRENDA, the Enzyme Database: 2.4.1.283

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