KEGG ENZYME: 2.4.1.284

ENZYME: 2.4.1.284

Entry

EC 2.4.1.284                Enzyme

Name

2-deoxystreptamine glucosyltransferase;
kanF (gene name)

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-alpha-D-glucose:2-deoxystreptamine 6-alpha-D-glucosyltransferase

Reaction(IUBMB)

UDP-alpha-D-glucose + 2-deoxystreptamine = UDP + 2'-deamino-2'-hydroxyparomamine [RN:R10096]

Reaction(KEGG)

R10096

Substrate

UDP-alpha-D-glucose [CPD:C00029];
2-deoxystreptamine [CPD:C02627]

Product

UDP [CPD:C00015];
2'-deamino-2'-hydroxyparomamine [CPD:C20353]

Comment

Involved in the biosynthesis of kanamycin B and kanamycin C. Also catalyses EC 2.4.1.283, 2-deoxystreptamine N-acetyl-D-glucosaminyltransferase, but activity is only one fifth of that with UDP-alpha-D-glucose.

History

EC 2.4.1.284 created 2012

Pathway

ec00524

Neomycin, kanamycin and gentamicin biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K13550

2-deoxystreptamine N-acetyl-D-glucosaminyltransferase / 2-deoxystreptamine glucosyltransferase

Genes

PALR:

HGI30_01160

SRN:	A4G23_05127(neoD)

SBRO:

GQF42_00970

SFB:	CP974_27360

MOM:	KUM42_19940

MSAG:

GCM10017556_22620

DVC:	Dvina_05405

Reference

1 [PMID:21983602]

Authors

Park JW, Park SR, Nepal KK, Han AR, Ban YH, Yoo YJ, Kim EJ, Kim EM, Kim D, Sohng JK, Yoon YJ

Title

Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation.

Journal

Nat Chem Biol 7:843-52 (2011)
DOI:10.1038/nchembio.671

Sequence

[ag:CAE46947]

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.284

IUBMB Enzyme Nomenclature:

2.4.1.284

ExPASy - ENZYME nomenclature database:

2.4.1.284

BRENDA, the Enzyme Database:

2.4.1.284

DBGET integrated database retrieval system