KEGG   ENZYME: 2.4.1.289
Entry
EC 2.4.1.289                Enzyme                                 

Name
N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase;
WbbL
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
dTDP-6-deoxy-beta-L-mannose:N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 3-alpha-L-rhamnosyltransferase
Reaction(IUBMB)
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol = dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol [RN:R10175 R12077]
Reaction(KEGG)
R10175 R12077(G)
Substrate
dTDP-6-deoxy-beta-L-mannose [CPD:C03319];
N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol [CPD:C20390]
Product
dTDP [CPD:C00363];
alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol [CPD:C20416]
Comment
Requires Mn2+ or Mg2+. Isolated from Mycobacterium smegmatis [1] and Mycobacterium tuberculosis [2]. The enzyme catalyses the addition of a rhamnosyl unit to N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol, completing the synthesis of the linkage unit that attaches the arabinogalactan moiety to the peptidoglycan moiety in Mycobacterial cell wall.
History
EC 2.4.1.289 created 2012
Pathway
ec00572  Arabinogalactan biosynthesis - Mycobacterium
ec01100  Metabolic pathways
Orthology
K16870  N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase
Genes
ECLO: ENC_41350
CSZ: CSSP291_05655
EAE: EAE_23495
CWE: CO701_07830
CEN: LH86_19190
ESC: Entcl_1663
KLE: AO703_13655
KSA: C813_00030
LAX: APT61_07785
LER: GNG29_14860
SMAF: D781_1501
SFJ: SAMEA4384070_1630(wbbL)
SMZ: SMD_0592
DKO: I596_626
PPF: Pput_2579
PPT: PPS_2792
PPI: YSA_10593
PPX: T1E_1088
PPUH: B479_13615
PPUT: L483_17790
PPUN: PP4_25070
PPUD: DW66_3050
PMON: X969_13315
PMOT: X970_12960
PSYA: AOT82_1808
MVS: MVIS_2692
MICC: AUP74_01015(wbbL_1) AUP74_01789(wbbL_2)
TIG: THII_3394
NMUS: H7A79_2358
RSE: F504_703
RSY: RSUY_09050(wbbL)
BVE: AK36_5111
BSEM: WJ12_18280
BGU: KS03_3657
BGO: BM43_6709
BUK: MYA_5168
LMIR: NCTC12852_01186(wbbL)
LIH: L63ED372_00866(wbbL_2)
HYB: Q5W_03940
HPSE: HPF_11020(wbbL1) HPF_17830(wbbL2) HPF_18175(wbbL3)
CBAA: SRAA_0329
CBAB: SMCB_0241
THI: THI_0363
BBAG: E1O_22640
AZA: AZKH_3759
DPI: BN4_10981
PPRF: DPRO_3001
DSF: UWK_02482
CCX: COCOR_04825(yefJ)
MHUA: MCHK_5868
AMIH: CO731_01225(wbbL_2)
PLA: Plav_3344
SMD: Smed_4788
ATF: Ach5_42970(wbbL)
RHT: NT26_3347
OAN: Oant_2736
NHA: Nham_2763
XAU: Xaut_4734
AZC: AZC_3113
MEX: Mext_0201
MCH: Mchl_0144
MNO: Mnod_5891
HDI: HDIA_1225(wbbL_2)
CSE: Cseg_1351
PZU: PHZ_c3342
TSV: DSM104635_03425(wbbL_2)
RUT: FIU92_20250(wbbL)
PGD: Gal_02971
LAQU: R2C4_03965
MALG: MALG_03345
RMM: ROSMUCSMR3_02656(wbbL)
ROH: FIU89_08990(wbbL)
RSU: NHU_04541
RHC: RGUI_4210
HNE: HNE_3194
NAR: Saro_3226
SWI: Swit_4473
SPHD: HY78_02950
SPHM: G432_03385
SPKC: KC8_08485
SSY: SLG_34240
ADO: A6F68_00900(wbbL)
ERF: FIU90_00315(wbbL)
SHUM: STHU_07840
SMN: SMA_0868(yefJ)
CCEL: CCDG5_0551
MTU: Rv3265c(wbbL1)
MTC: MT3365
MRA: MRA_3306(wbbL1)
MTUR: CFBS_3456(wbbL1)
MTO: MTCTRI2_3332(wbbL1)
MTD: UDA_3265c(wbbL1)
MTN: ERDMAN_3581(wbbL1)
MTUB: MT7199_3307(wbbL1)
MTUH: I917_22935
MTUL: TBHG_03201
MTUT: HKBT1_3443(wbbL1)
MTUU: HKBT2_3450(wbbL1)
MTQ: HKBS1_3453(wbbL1)
MBO: BQ2027_MB3293C(wbbL1)
MBB: BCG_3294c(wbbL1)
MBT: JTY_3290(wbbL1)
MBM: BCGMEX_3292c(wbbL1)
MBX: BCGT_3133
MAF: MAF_32760(wbbL1)
MMIC: RN08_3601
MCE: MCAN_32841(wbbL1)
MLE: ML0752(wbbL)
MLB: MLBr00752(wbbL)
MPA: MAP_3379c(wbbL)
MAO: MAP4_0403
MAVU: RE97_01935
MAV: MAV_4230
MIT: OCO_40880
MIA: OCU_40790
MID: MIP_06155
MYO: OEM_41140
MIR: OCQ_42150
MLP: MLM_3388
MMAN: MMAN_28930(wbbL)
MUL: MUL_2611(wbbL1)
MMC: Mmcs_1320
MKM: Mkms_1337
MJL: Mjls_1356
MMI: MMAR_1276(wbbL1)
MMAE: MMARE11_12420(wbbL1)
MMM: W7S_20410
MLI: MULP_01437(wbbL)
MHAD: B586_17520
MSHG: MSG_01176(wbbL)
MFJ: MFLOJ_22120(wbbL)
MSTO: MSTO_25840(wbbL)
MSIM: MSIM_13130(wbbL)
MSAK: MSAS_48280(wbbL)
MXE: MYXE_37130(wbbL)
MNV: MNVI_28140(wbbL)
MNM: MNVM_30530(wbbL)
MCOO: MCOO_44600(wbbL)
MBAI: MB901379_01167(wbbL)
MSEO: MSEO_51080(wbbL)
MPSE: MPSD_14540(wbbL)
MSHO: MSHO_60340(wbbL)
MHEK: JMUB5695_03658(wbbL)
MLJ: MLAC_04450(wbbL)
MBRD: MBRA_03650(wbbL)
MSHJ: MSHI_24960(wbbL)
MKR: MKOR_20250(wbbL)
MSG: MSMEI_1783(wbbL1) MSMEI_5801
MVA: Mvan_1728
MGI: Mflv_4735
MFT: XA26_18620(wbbL)
MPHL: MPHLCCUG_01623(wbbL)
MVQ: MYVA_1472
MTHN: 4412656_01183(wbbL)
MHAS: MHAS_00500(wbbL)
MCHT: MCHIJ_50240(wbbL)
MAUU: NCTC10437_01435(wbbL)
MMAG: MMAD_14790
MMOR: MMOR_51250
MAIC: MAIC_13000(wbbL)
MALV: MALV_19590
MTY: MTOK_35100(wbbL)
MARZ: MARA_24330
MGAD: MGAD_25290
MHEV: MHEL_36200(wbbL)
MSAR: MSAR_04280(wbbL)
MANY: MANY_52640(wbbL1)
MPOF: MPOR_20130
MPHU: MPHO_54200
MBOK: MBOE_56040
MAB: MAB_3612c
MABB: MASS_3619
MCHE: BB28_18475
MSTE: MSTE_03745
MSAL: DSM43276_03409(wbbL_2)
MJD: JDM601_3016(wbbL1)
MTER: 4434518_03002(wbbL1)
MMIN: MMIN_07460(wbbL1)
MHIB: MHIB_24590(wbbL)
ASD: AS9A_3814(wbbL)
CGL: Cgl0741(Cgl0741)
CGB: cg0848(wbbL)
CGU: WA5_0709(WbbL)
CGT: cgR_0850
CGM: cgp_0848(wbbL)
CGJ: AR0_04280
CEF: CE0755
CDI: DIP0681
CDP: CD241_0620(wbbL)
CDH: CDB402_0594(wbbL)
CDT: CDHC01_0619(wbbL)
CDE: CDHC02_0625(wbbL)
CDR: CDHC03_0604(wbbL)
CDA: CDHC04_0585(wbbL)
CDZ: CD31A_0684(wbbL)
CDB: CDBH8_0642(wbbL)
CDS: CDC7B_0635(wbbL)
CDD: CDCE8392_0629(wbbL)
CDW: CDPW8_0681(wbbL)
CDV: CDVA01_0567(wbbL)
CDIP: ERS451417_00617(wbbL)
CJK: jk1646
CUR: cu0470
CUA: CU7111_0463(wbbL)
CAR: cauri_0658(wbbL)
CKP: ckrop_1519(wbbL)
CPL: Cp3995_0509(wbbL)
CPP: CpP54B96_0508(wbbL)
CPZ: CpPAT10_0504(wbbL)
COR: Cp267_0523(wbbL)
COD: Cp106_0491(wbbL)
COS: Cp4202_0495(wbbL)
CPSE: CPTA_01050
CPSU: CPTB_00483
CPSF: CPTC_00143
CRD: CRES_1734(wbbL)
CUL: CULC22_00561(wbbL)
CUC: CULC809_00554(wbbL)
CUN: Cul210932_0583(wbbL)
CUS: CulFRC11_0556(wbbL)
CUQ: Cul210931_0560(wbbL)
CUZ: Cul05146_0597(wbbL)
CUJ: CUL131002_0560(wbbL)
CVA: CVAR_2183
CTER: A606_08780
CGY: CGLY_04515(wbbL)
COA: DR71_1586(wbbL)
CSX: CSING_03665(wbbL)
CMQ: B840_02670(wbbL)
CKU: UL82_02350(wbbL)
CCJ: UL81_03050(wbbL)
CMV: CMUST_03800(wbbL)
CEI: CEPID_03205(wbbL)
CTED: CTEST_03085(wbbL)
CDX: CDES_03740(wbbL)
CSP: WM42_0714(wbbL)
CPHO: CPHO_09630
CGV: CGLAU_02795(wbbL)
CAQU: CAQU_02835
CAMG: CAMM_10040
CMIN: NCTC10288_01877(wbbL)
CPEG: CPELA_08425(wbbL)
CEE: CENDO_02825(wbbL)
CGK: CGERO_02665(wbbL)
CRL: NCTC7448_01374(wbbL)
CCHO: CCHOA_02425(wbbL)
CPRE: Csp1_07760(wbbL)
CPSO: CPPEL_08660(wbbL)
CCYS: SAMEA4530656_1783(wbbL)
CUO: CUROG_08080(wbbL)
NFA: NFA_46370
NFR: ERS450000_01716(wbbL_1) ERS450000_05291(wbbL_3)
NSR: NS506_02058(wbbL)
NAD: NCTC11293_05945(wbbL)
RER: RER_21480(wbbL)
REY: O5Y_10260
ROP: ROP_63700(wbbL)
REQ: REQ_33680
RHB: NY08_1687
RFA: A3L23_00051(wbbL_1) A3L23_01234(wbbL_2) A3L23_01251(wbbL_3)
RHS: A3Q41_03362(wbbL)
RHU: A3Q40_02038(wbbL)
RRT: 4535765_03356(wbbL) 4535765_03902(hyaD_3)
RCR: NCTC10994_03901(wbbL)
GBR: Gbro_1854
GOR: KTR9_1791
GRU: GCWB2_09095(wbbL)
GOM: D7316_01397(wbbL)
TPR: Tpau_1063
SRT: Srot_2674
SMAL: SMALA_0583
SLAU: SLA_0636
SGE: DWG14_01338(wbbL)
SNF: JYK04_06575(wbbL_2)
TWH: TWT_033(wbbL)
TWS: TW037
LXX: Lxx04940(wbbL)
MOO: BWL13_00217(wbbL_1) BWL13_02457(wbbL_2)
MOY: CVS54_00662(wbbL)
AUW: AURUGA1_01208(wbbL_1)
AGX: AGREI_0817(wbbL)
KRH: KRH_14550
BCV: Bcav_0993
LMOI: VV02_08775
XCE: Xcel_2693
CFL: Cfla_2344
SERJ: SGUI_2428
ACIJ: JS278_01520(wbbL_1) JS278_02539(wbbL_2)
NDK: I601_1631
SRO: Sros_0657
ACE: Acel_1912
KRA: Krad_0566
SEN: SACE_6472(wbbL)
SACC: EYD13_03445(wbbL2)
AMD: AMED_1014
AMN: RAM_05160
AMM: AMES_1011
AMZ: B737_1012
AOI: AORI_0999
AJA: AJAP_34580(wbbL)
AMQ: AMETH_0925(wbbL)
AMYY: YIM_42600(wbbL3) YIM_44945(wbbL6)
AORI: SD37_36190
PDX: Psed_5212
PSEA: WY02_11535
PSEH: XF36_02080
PAUT: Pdca_56480
AMI: Amir_6339
SESP: BN6_76290(gtuS2-20)
AHG: AHOG_24550(wbbL2) AHOG_26560(wbbL4)
ALO: CRK59738
ASE: ACPL_3102
ACTN: L083_3056
ACTS: ACWT_2975
CAI: Caci_8508
AHE: Arch_0059
TPYO: X956_02545
TBW: NCTC13354_00633(wbbL)
AHW: NCTC11636_02341(wbbL_2)
AIR: NCTC12972_00180(wbbL_1) NCTC12972_00196(wbbL_2)
ASLA: NCTC11923_00261(wbbL_1) NCTC11923_02582(wbbL_4)
AVC: NCTC10951_01509(wbbL_1) NCTC10951_01539(wbbL_2) NCTC10951_02640(wbbL_3)
CWO: Cwoe_5307
AFO: Afer_0736
SYZ: MYO_2440
LET: O77CONTIG1_00521(wbbL)
MAR: MAE_41900
MPK: VL20_3649
CYT: cce_4724
NSH: GXM_02809
HAU: Haur_4741
TRO: trd_1593
TRA: Trad_1109
PNL: PNK_0186
PUV: PUV_27670
WCH: wcw_1805
OTE: Oter_1773
OBG: Verru16b_01617(wbbL_2)
PND: Pla175_27360(wbbL)
PLH: VT85_20605(wbbL_1) VT85_21315(wbbL_3) VT85_25680(wbbL_4)
SDYN: Mal52_62140(wbbL_2)
PLON: Pla110_36700(wbbL)
GES: VT84_15320(wbbL_2) VT84_36600(wbbL_5)
FTJ: FTUN_2978
ULI: ETAA1_43160(wbbL_3)
PBOR: BSF38_00474(wbbL_1) BSF38_04482
AGV: OJF2_27170(wbbL_1) OJF2_62640(wbbL_3) OJF2_64570(wbbL_5)
SUS: Acid_5285
ABAC: LuPra_03147(wbbL_1)
FSC: FSU_2573
DDO: I597_2729(wbbL_2)
FBA: FIC_01554
CGLE: NCTC11432_03668(wbbL_2) NCTC11432_04428(wbbL_3)
CTAI: NCTC12078_02655(wbbL_2) NCTC12078_03334(wbbL_3)
CTAK: 4412677_02443(wbbL)
CJG: NCTC13459_01810(wbbL_2)
PROC: Ptc2401_00332(wbbL_1)
TNE: Tneu_0484
 » show all
Reference
1  [PMID:15294902]
  Authors
Mills JA, Motichka K, Jucker M, Wu HP, Uhlik BC, Stern RJ, Scherman MS, Vissa VD, Pan F, Kundu M, Ma YF, McNeil M
  Title
Inactivation of the mycobacterial rhamnosyltransferase, which is needed for the formation of the arabinogalactan-peptidoglycan linker, leads to irreversible loss of viability.
  Journal
J Biol Chem 279:43540-6 (2004)
DOI:10.1074/jbc.M407782200
  Sequence
[mtu:Rv3265c]
Reference
2  [PMID:19047740]
  Authors
Grzegorzewicz AE, Ma Y, Jones V, Crick D, Liav A, McNeil MR
  Title
Development of a microtitre plate-based assay for lipid-linked glycosyltransferase products using the mycobacterial cell wall rhamnosyltransferase WbbL.
  Journal
Microbiology 154:3724-30 (2008)
DOI:10.1099/mic.0.2008/023366-0
  Sequence
[mtu:Rv3265c]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.289
IUBMB Enzyme Nomenclature: 2.4.1.289
ExPASy - ENZYME nomenclature database: 2.4.1.289
BRENDA, the Enzyme Database: 2.4.1.289

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