KEGG   ENZYME: 2.4.1.301Help
Entry
EC 2.4.1.301                Enzyme                                 

Name
2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase;
kanE (gene name);
kanM2 (gene name)
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-alpha-D-kanosamine:2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase
Reaction(IUBMB)
(1) UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyneamine = UDP + kanamycin A [RN:R10424];
(2) UDP-alpha-D-kanosamine + neamine = UDP + kanamycin B [RN:R10425];
(3) UDP-alpha-D-kanosamine + paromamine = UDP + kanamycin C [RN:R10426];
(4) UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyparomamine = UDP + kanamycin X [RN:R10427]
Reaction(KEGG)
Substrate
UDP-alpha-D-kanosamine [CPD:C12211];
2'-deamino-2'-hydroxyneamine [CPD:C20350];
neamine [CPD:C01441];
paromamine [CPD:C01743];
2'-deamino-2'-hydroxyparomamine [CPD:C20353]
Product
UDP [CPD:C00015];
kanamycin A [CPD:C01822];
kanamycin B [CPD:C00825];
kanamycin C [CPD:C01823];
kanamycin X [CPD:C20614]
Comment
Involved in the biosynthetic pathway of kanamycins. The enzyme characterized from the bacterium Streptomyces kanamyceticus can also accept UDP-alpha-D-glucose with lower efficiency [2].
History
EC 2.4.1.301 created 2013
Pathway
ec00524  Neomycin, kanamycin and gentamicin biosynthesis
ec01110  Biosynthesis of secondary metabolites
ec01130  Biosynthesis of antibiotics
Orthology
K20573  2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase
Reference
1  [PMID:19911031]
  Authors
Kudo F, Sucipto H, Eguchi T
  Title
Enzymatic activity of a glycosyltransferase KanM2 encoded in the kanamycin biosynthetic gene cluster.
  Journal
J Antibiot (Tokyo) 62:707-10 (2009)
DOI:10.1038/ja.2009.107
Reference
2  [PMID:21983602]
  Authors
Park JW, Park SR, Nepal KK, Han AR, Ban YH, Yoo YJ, Kim EJ, Kim EM, Kim D, Sohng JK, Yoon YJ
  Title
Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation.
  Journal
Nat Chem Biol 7:843-52 (2011)
DOI:10.1038/nchembio.671
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.301
IUBMB Enzyme Nomenclature: 2.4.1.301
ExPASy - ENZYME nomenclature database: 2.4.1.301
BRENDA, the Enzyme Database: 2.4.1.301

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