KEGG   ENZYME: 2.4.1.315Help
Entry
EC 2.4.1.315                Enzyme                                 

Name
diglucosyl diacylglycerol synthase (1,6-linking);
monoglucosyl diacylglycerol (1->6) glucosyltransferase;
MGlcDAG (1->6) glucosyltransferase;
DGlcDAG synthase (ambiguous);
UGT106B1;
ypfP (gene name)
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-alpha-D-glucose:1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol 6-glucosyltransferase
Reaction(IUBMB)
(1) UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-[beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl]-sn-glycerol + UDP [RN:R04377];
(2) UDP-alpha-D-glucose + 1,2-diacyl-3-O-[beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl]-sn-glycerol = 1,2-diacyl-3-O-[beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl]-sn-glycerol + UDP [RN:R10807]
Reaction(KEGG)
R04377 R10807;
(other) R02689
Show
Substrate
UDP-alpha-D-glucose [CPD:C00029];
1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol [CPD:C04046];
1,2-diacyl-3-O-[beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl]-sn-glycerol [CPD:C06040]
Product
1,2-diacyl-3-O-[beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl]-sn-glycerol [CPD:C06040];
UDP [CPD:C00015];
1,2-diacyl-3-O-[beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl]-sn-glycerol [CPD:C20860]
Comment
The enzyme is found in several bacterial species. The enzyme from Bacillus subtilis is specific for glucose [1]. The enzyme from Mycoplasma genitalium can incoporate galactose with similar efficiency, but forms mainly 1,2-diacyl-diglucopyranosyl-sn-glycerol in vivo [3]. The enzyme from Staphylococcus aureus can also form glucosyl-glycero-3-phospho-(1'-sn-glycerol) [2].
History
EC 2.4.1.315 created 2014
Pathway
ec00561  Glycerolipid metabolism
ec01100  Metabolic pathways
Orthology
K03429  processive 1,2-diacylglycerol beta-glucosyltransferase
K19004  processive 1,2-diacylglycerol beta-glycosyltransferase
Genes
DKO: I596_2961
LRZ: BJI69_00355
PRR: AT705_22950
CELL: CBR65_15955
SDF: ACG33_01070
RIN: ACS15_0529
CBW: RR42_s2098
CUP: BKK80_10135
CUU: BKK79_02985
BUR: Bcep18194_B0550
BCEN: DM39_5517
BCON: NL30_00785
BUB: BW23_3502
BPSL: WS57_02480
BMEC: WJ16_30065
CFU: CFU_4282(ugtP)
CARE: LT85_4882
DPB: BABL1_gene_141(ugtP_2) BABL1_gene_142(ugtP_1)
BSU: BSU21920(ugtP)
BSR: I33_2251
BSL: A7A1_2878
BSH: BSU6051_21920(ugtP)
BSUT: BSUB_02357(ugtP)
BSUL: BSUA_02357(ugtP)
BSUS: Q433_11940
BSS: BSUW23_10730(ugtP)
BST: GYO_2415
BSO: BSNT_08581(ugtP)
BSQ: B657_21920(ugtP)
BSX: C663_2064(ugtP)
BLI: BL01366(ugtP)
BLD: BLi02330(ugtP)
BLH: BaLi_c24180(ugtP)
BAY: RBAM_020060(ugtP)
BAQ: BACAU_2012(ugtP)
BYA: BANAU_2112(ugtP)
BAMP: B938_10370
BAML: BAM5036_1934(ugtP)
BAMA: RBAU_1970(ugtP)
BAMN: BASU_1937(ugtP)
BAMB: BAPNAU_1723(ugtP)
BAMT: AJ82_11385
BAMY: V529_22110(ugtP)
BMP: NG74_02099(ugtP)
BAO: BAMF_2092(ugtP)
BAZ: BAMTA208_07035(ugtP)
BQL: LL3_02197(ugtP)
BXH: BAXH7_01431(ugtP)
BQY: MUS_2368(ugtP)
BAMI: KSO_009405
BAMC: U471_20680
BAMF: U722_10970
BATR: TD68_07965
BHA: BH1957
BAN: BA_0511
BAR: GBAA_0511
BAT: BAS0483
BAI: BAA_0572
BANT: A16_05500
BANR: A16R_05560
BANS: BAPAT_0487
BANV: DJ46_4949(ugtP)
BCE: BC0493
BCA: BCE_0565
BCZ: BCE33L0422(ugtP)
BCQ: BCQ_0539(ugtP)
BCX: BCA_0530
BAL: BACI_c05050(ugtP)
BNC: BCN_0488
BCF: bcf_02500
BCER: BCK_05575
BTK: BT9727_0426(ugtP)
BTL: BALH_0448(ugtP)
BTT: HD73_0580
BTHI: BTK_02950
BTM: MC28_5237(ugtP)
BTG: BTB_c05070(ugtP)
BTI: BTG_18615
BTW: BF38_1741(ugtP)
BWW: bwei_5274(ugtP)
BMYC: DJ92_3086(ugtP)
BMYO: BG05_19(ugtP)
BPU: BPUM_1928
BPUM: BW16_10550
BPUS: UP12_09840
BPF: BpOF4_01985(ugtP)
BMQ: BMQ_1671(ugtP)
BMD: BMD_1644(ugtP)
BMEG: BG04_3955
BAG: Bcoa_2253
BCOA: BF29_1323
BJS: MY9_2195
BACW: QR42_09795
BACP: SB24_18670
BACB: OY17_13065
BACY: QF06_09165
BACL: BS34A_24160(ugtP)
BALM: BsLM_2134
BGY: BGLY_2585(ugtP)
AAMY: GFC30_2324
SAU: SA0875
SAV: SAV1017
SAW: SAHV_1011
SAM: MW0898
SAS: SAS0886
SAR: SAR0987
SAC: SACOL1022(ypfP)
SAE: NWMN_0887(murG)
SAD: SAAV_0979(ypfP)
SUJ: SAA6159_00873(ypfP)
SUK: SAA6008_00972(ypfP)
SUC: ECTR2_872
SUZ: MS7_0973(ugtP)
SUW: SATW20_10140(ugtP)
SUG: SAPIG1013
SUF: SARLGA251_09320(ugtP)
SAUA: SAAG_02126
SAUE: RSAU_000902(ugtP)
SAUS: SA40_0887(ugtP)
SAUU: SA957_0902(ugtP)
SAUG: SA268_0904(ugtP)
SAUZ: SAZ172_0958(ugtP)
SAUT: SAI1T1_2007190(ugtP)
SAUJ: SAI2T2_1007210(ugtP)
SAUK: SAI3T3_1007200(ugtP)
SAUQ: SAI4T8_1007190(ugtP)
SAUV: SAI7S6_1007200(ugtP)
SAUW: SAI5S5_1007160(ugtP)
SAUX: SAI6T6_1007170(ugtP)
SAUY: SAI8T7_1007200(ugtP)
SAUF: X998_1015
SAB: SAB0883c
SAUB: C248_1041
SAUM: BN843_9220
SAUC: CA347_934
SAUR: SABB_00985(ugtP)
SAUI: AZ30_04835
SAUD: CH52_00800
SAMS: NI36_04840
SEP: SE0717
SER: SERP0606(ypfP)
SEPP: SEB_00696
SEPS: DP17_2002
SHA: SH1942
SHH: ShL2_01804(ypfP)
SSP: SSP1768
SCA: SCA_0619
SDT: SPSE_1820
SPAS: STP1_2086
SXO: SXYL_01915(ugtP)
SHU: SHYC_09255(ugtP)
SCAP: AYP1020_0274(ugtP)
SSCH: LH95_08710
SSCZ: RN70_09190
SAGQ: EP23_00735
MCL: MCCL_0630(murG)
MCAK: MCCS_08550(ugtP)
PPM: PPSC2_03160(ugtP1) PPSC2_07010(ypfP) PPSC2_12770(ugtP3) PPSC2_12780(ugtP5)
PPO: PPM_0574(ugtP1) PPM_1330(ypfP) PPM_2448(ugtP3) PPM_2450(ugtP5)
ASOC: CB4_01251(ugtP)
AAC: Aaci_1913
AAD: TC41_2011(ugtP)
CRN: CAR_c06310(ugtP)
CARC: NY10_2328
CAC: CA_C2897
CAE: SMB_G2933(ugtP)
CAY: CEA_G2904
CPR: CPR_2180
CTC: CTC_00289
CBO: CBO0127
CBA: CLB_0163
CBH: CLC_0175
CBY: CLM_0170
CBL: CLK_3302
CBK: CLL_A0478
CBB: CLD_0659
CBI: CLJ_B0165
CBT: CLH_0470
CBF: CLI_0182
CBM: CBF_0155
CBE: Cbei_0400
CBZ: Cbs_0400
CBEI: LF65_00458
CKL: CKL_3719(murG2)
CKR: CKR_3284
CSR: Cspa_c06070(ugtP)
CPAT: CLPA_c02520(ugtP1) CLPA_c32970(ugtP2)
CPAE: CPAST_c02520(ugtP1) CPAST_c32970(ugtP2)
CSB: CLSA_c04970(ugtP)
CBV: U729_1610
CLD: CLSPO_c01520(ugtP)
AOE: Clos_0260
ASF: SFBM_1287
CTH: Cthe_2414
RBR: RBR_07710
HSC: HVS_00215(ugtP)
RIX: RO1_38260
RIM: ROI_16830
CPY: Cphy_1892
PDC: CDIF630_00034(ugtP1) CDIF630_01500(ugtP2)
EAC: EAL2_c00420(ugtP1) EAL2_c20400(ugtP2)
STH: STH752
SLP: Slip_0547
DRM: Dred_1966
PTH: PTH_1264(MurG)
SGY: Sgly_2492
HMO: HM1_2271
AWO: Awo_c16820(ugtP) Awo_c19680(murG2)
TMR: Tmar_1675
SAY: TPY_3607(ugtP)
CTHM: CFE_2352
ADG: Adeg_0646
TPZ: Tph_c22650(ugtP)
CSC: Csac_2337
ATE: Athe_1794
TOC: Toce_1869
TTM: Tthe_0231
TSH: Tsac_0789
PMIC: NW74_06445
VPR: Vpar_1358
VRM: 44547418_01438(ugtP)
MED: MELS_1791
SRI: SELR_21780(ugtP)
LPIL: LIP_2710
MGE: MG_517
MGU: CM5_01980
MGC: CM9_01990
MGQ: CM3_02110
MGX: CM1_02020
MPN: MPN483(yibD)
MPM: MPNA4830
MPJ: MPNE_0563
MPB: C985_0491
AAU: AAur_1901
BLIN: BLSMQ_1922
MPH: MLP_49230
ELE: Elen_1241
AEQ: AEQU_0868
HHG: XM38_029720(ugtP)
GVI: glr3229
GLJ: GKIL_1939(ugtP)
RCA: Rcas_2476
CAU: Caur_0652
CAG: Cagg_3363
DRA: DR_1076
DGE: Dgeo_1184
TRA: Trad_2896
OBG: Verru16b_01989(ugtP_1) Verru16b_02298(ugtP_2)
AGL: PYTT_0628
XII: AMD24_00201(ugtP)
MIN: Minf_0951(murG)
SACI: Sinac_0207
TPED: TPE_1929
BPO: BP951000_0939(murG2)
BPW: WESB_2265
BIP: Bint_1023
TAI: Taci_1764
TLI: Tlie_1885
LFC: LFE_1921
 » show all
Taxonomy
Reference
1  [PMID:9720862]
  Authors
Jorasch P, Wolter FP, Zahringer U, Heinz E
  Title
A UDP glucosyltransferase from Bacillus subtilis successively transfers up to four glucose residues to 1,2-diacylglycerol: expression of ypfP in Escherichia coli and structural analysis of its reaction products.
  Journal
Mol Microbiol 29:419-30 (1998)
DOI:10.1046/j.1365-2958.1998.00930.x
  Sequence
[bsu:BSU21920]
Reference
2  [PMID:10848996]
  Authors
Jorasch P, Warnecke DC, Lindner B, Zahringer U, Heinz E
  Title
Novel processive and nonprocessive glycosyltransferases from Staphylococcus aureus and Arabidopsis thaliana synthesize glycoglycerolipids, glycophospholipids, glycosphingolipids and glycosylsterols.
  Journal
Eur J Biochem 267:3770-83 (2000)
DOI:10.1046/j.1432-1327.2000.01414.x
  Sequence
[sac:SACOL1022]
Reference
3  [PMID:21835921]
  Authors
Andres E, Martinez N, Planas A
  Title
Expression and characterization of a Mycoplasma genitalium glycosyltransferase in membrane glycolipid biosynthesis: potential target against mycoplasma infections.
  Journal
J Biol Chem 286:35367-79 (2011)
DOI:10.1074/jbc.M110.214148
  Sequence
[mge:MG_517]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.315
IUBMB Enzyme Nomenclature: 2.4.1.315
ExPASy - ENZYME nomenclature database: 2.4.1.315
BRENDA, the Enzyme Database: 2.4.1.315

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